Article
Biochemistry & Molecular Biology
Mattia Albiero, Marianna D'Anna, Benedetta Maria Bonora, Gaia Zuccolotto, Antonio Rosato, Marco Giorgio, Elisabetta Iori, Angelo Avogaro, Gian Paolo Fadini
Summary: For the first time, this study explores the hematopoietic and nonhematopoietic roles of p66Shc in regulating HSPC traffic and ischemic responses. Hematopoietic deletion of p66Shc is sufficient to rescue HSPC mobilization and homing in diabetes after ischemia and improves blood flow recovery. Inhibiting p66Shc in blood cells may be a novel strategy to counter PAD in diabetes.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Chemistry, Multidisciplinary
Ya Guan, Ning Gao, Hong Niu, Yu Dang, Jianjun Guan
Summary: The developed oxygen-release microspheres have the potential to enhance cell survival in ischemic tissues by improving oxygen supply, promoting angiogenesis, and tissue regeneration in a safer and more efficient manner. The microspheres are responsive to environmental oxygen levels, controlling oxygen release and significantly improving the therapeutic effects of cell transplantation under hypoxic conditions.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Hyeok Kim, Soon-Jung Park, Jae-Hyun Park, Sunghun Lee, Bong-Woo Park, Soon Min Lee, Ji-Won Hwang, Jin-Ju Kim, Byeongmin Kang, Woo-Sup Sim, Hyo-Jin Kim, Seung Hwan Jeon, Dong-Bin Kim, Jinah Jang, Dong-Woo Cho, Sung-Hwan Moon, Hun-Jun Park, Kiwon Ban
Summary: Researchers have successfully used a combination of two different types of stem cells to repair failed hearts by creating new blood vessels. This dual stem cell treatment improves cardiac function and results in a higher number of larger and stronger new blood vessels.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Cell & Tissue Engineering
Qi Liu, Su Pan, Shijie Liu, Sui Zhang, James T. Willerson, James F. Martin, Richard A. F. Dixon
Summary: Targeting the Hippo pathway in muscles can promote angiogenesis and myogenesis, leading to improved functional recovery and increased exercise endurance in ischemic muscles. Knockdown of SAV1 enhances proliferation of muscle satellite cells and endothelial cells, playing a crucial role in skeletal muscle regeneration.
Article
Cell & Tissue Engineering
Franka Messner, Marco Thurner, Jule Mueller, Michael Blumer, Julia Hofmann, Rainer Marksteiner, Sebastien Couillard-Despres, Jakob Troppmair, Dietmar Oefner, Stefan Schneeberger, Theresa Hautz
Summary: The study established a model of local application of myogenic progenitor cells (MPC) in murine hindlimb ischemia/reperfusion to study cell engraftment and differentiation for muscle regeneration. The results showed that injected MPC had the ability to engraft and differentiate into muscle fibers in IRI-affected muscle, indicating a potential therapeutic strategy for antagonizing muscle damage caused by IRI. Further research is needed to evaluate the regenerative capacity and therapeutic benefit of MPC in ischemic limb injury.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Ashok Kumar, Vihang A. Narkar
Summary: This review proposes nuclear receptors as potential candidates for ischemic muscle repair in peripheral arterial disease (PAD), focusing on their roles in muscle angiogenesis, oxidative metabolism, mitochondrial biogenesis, and muscle regeneration. The article also identifies gaps in our understanding of nuclear receptor signaling in skeletal muscle and suggests future research directions.
Article
Cardiac & Cardiovascular Systems
Hasan Abbas, Lindsey A. Olivere, Michael E. Padgett, Cameron A. Schmidt, Brian F. Gilmore, Timothy J. McCord, Kevin W. Southerland, Joseph M. McClung, Christopher D. Kontos
Summary: Skeletal muscle injury in peripheral artery disease (PAD) is not only attributed to vascular insufficiency, but also to muscle cell responses. Genetic ablation of Pax7(+) muscle progenitor cells (MPCs) in a hindlimb ischemia model led to inhibited muscle regeneration. Adipogenesis and fibrosis were observed in the ischemic limb, and inhibition of fibro/adipogenic progenitors (FAPs) decreased adipose but increased fibrosis. Patients with critical limb threatening ischemia also showed increased adipose and decreased satellite cells in more ischemic regions. These findings suggest that muscle regeneration could be a potential therapeutic target in PAD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Cell Biology
Marion Mesnieres, Anna-Marei Bohm, Nicolas Peredo, Dana Trompet, Roger Valle-Tenney, Manmohan Bajaj, Nikky Corthout, Elena Nefyodova, Ruben Cardoen, Pieter Baatsen, Sebastian Munck, Andras Nagy, Jody J. Haigh, Satish Khurana, Catherine M. Verfaillie, Christa Maes
Summary: The balanced local crosstalk within the skeletal niche is crucial for the successful settlement of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow, with overproduction of VEGF by osteoprogenitor cells impacting stromal-vessel interface and vascular architecture, resulting in impaired HSPC homing and survival. These findings highlight potential targets for improving stem cell therapies, as similar osteo-angiogenic niche changes are induced by irradiation in adult mice.
Article
Chemistry, Multidisciplinary
Lai-liu Luo, Jing-xuan Han, Shou-rong Wu, Vivi Kasim
Summary: This study suggests that SGLT2 inhibitor sotagliflozin may serve as a potential therapeutic angiogenesis agent for diabetic hindlimb ischemia (HLI). The results demonstrate that sotagliflozin can reverse the detrimental effects of high glucose and hypoxia on skeletal muscle cells, increase the expression and secretion of angiogenic factors, enhance the migration and proliferation capabilities of vascular endothelial and smooth muscle cells, and promote the formation of functional blood vessels. In vivo experiments show that intramuscular injection of sotagliflozin effectively restores blood perfusion in diabetic HLI mice.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Multidisciplinary Sciences
Yoshiya Asano, Daisuke Okano, Michiya Matsusaki, Tetsuro Watabe, Yasuhiro Yoshimatsu, Mitsuru Akashi, Hiroshi Shimoda
Summary: In this study, an artificial vascular tissue constructed by human adipose tissue-derived stromal cells (hASCs) and HUVECs (ASCVT) showed higher vascular network density and expression of angiogenesis-related factors. Subcutaneous transplantation of ASCVT into nude mice resulted in the formation of functional vessel networks within 4 weeks.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Cheng Peng, Li-jun Yang, Chuan Zhang, Yu Jiang, Liu-wen-xin Shang, Jia-bei He, Zhen-wei Zhou, Xia Tao, Lu Tie, Alex F. Chen, He-hui Xie
Summary: Low-dose nifedipine has been found to enhance the angiogenic potential of endothelial progenitor cells (EPCs) and protect against cerebral ischemic injury in diabetic mice. The treatment with nifedipine significantly increased the number of circulating EPCs, improved EPC function, and promoted angiogenesis. It also reduced the levels of inhibitory factors in EPCs. This study suggests that long-term treatment with low-dose nifedipine can be a safe and cost-effective strategy to prevent ischemic diseases in diabetes.
ACTA PHARMACOLOGICA SINICA
(2023)
Review
Pharmacology & Pharmacy
Yasaman Aghazadeh, Safwat T. Khan, Blessing Nkennor, Sara S. Nunes
Summary: Cell-based vascularization strategies show clinical potential but face challenges and obstacles.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Nevin Witman, Chikai Zhou, Timm Haeneke, Yao Xiao, Xiaoting Huang, Eduarde Rohner, Jesper Sohlmer, Niels Grote Beverborg, Kenneth R. Chien, Makoto Sahara
Summary: Cardiogenic growth factors, such as PLGF, play crucial roles in heart development. PLGF exhibits both autocrine and paracrine functions during cardiomyogenesis and vasculogenesis, and it may have potential therapeutic applications for heart disease. Further studies have revealed the previously unrecognized transcriptional networks related to PLGF, driven by EOMES and SOX17.
NATURE COMMUNICATIONS
(2023)
Review
Pharmacology & Pharmacy
Shigang Lin, Ruoran Lin, Hongkun Zhang, Qingbo Xu, Yangyan He
Summary: This review focuses on the functional changes of peripheral arterial cells and the mechanisms of PAD pathogenesis. It summarizes the progress of current clinical treatment and potential therapeutic methods for PAD, and provides insights for future perspectives.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Sabrina Summer, Eva Rossmanith, Markus Pasztorek, Constantin Fiedler, Marion Groeger, Sabine Rauscher, Viktoria Weber, Michael B. Fischer
Summary: Mesenchymal stem cells play a crucial role in supporting endothelial cells during tissue regeneration and new vessel formation. By co-culturing these cells in a specific microenvironment, complex vascular networks can be formed. Under dynamic flow conditions, mesenchymal stem cells can bind to damaged endothelial cells, potentially aiding in the healing process.
Article
Pathology
Cameron A. Schmidt, Adam J. Amorese, Terence E. Ryan, Emma J. Goldberg, Michael D. Tarpey, Thomas D. Green, Reema R. Karnekar, Dean J. Yamaguchi, Espen E. Spangenburg, Joseph M. McClung
AMERICAN JOURNAL OF PATHOLOGY
(2018)
Article
Cell & Tissue Engineering
Aleksandra Tata, Yoshihiko Kobayashi, Ryan D. Chow, Jasmine Tran, Avani Desai, Abdull J. Massri, Timothy J. McCord, Michael Dee Gunn, Purushothama Rao Tata
Article
Oncology
Claire J. Detweiler, Sarah B. Mueller, Anthony D. Sung, Jennifer L. Saullo, Vinod K. Prasad, Diana M. Cardona
JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY
(2018)
Article
Cell Biology
Michael D. Tarpey, Adam J. Amorese, Nicholas P. Balestrieri, Terence E. Ryan, Cameron A. Schmidt, Joseph M. McClung, Espen E. Spangenburg
Article
Neurosciences
Brenton T. Laing, Peixin Li, Cameron A. Schmidt, Wyatt Bunner, Yuan Yuan, Taylor Landry, Amber Prete, Joseph M. McClung, Hu Huang
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Cameron A. Schmidt, Emma J. Goldberg, Tom D. Green, Reema R. Karnekar, Jeffrey J. Brault, Spencer G. Miller, Adam J. Amorese, Dean J. Yamaguchi, Espen E. Spangenburg, Joseph M. McClung
Article
Peripheral Vascular Disease
Zoe S. Terwilliger, Terence E. Ryan, Emma J. Goldberg, Cameron A. Schmidt, Dean J. Yamaguchi, Reema Karnekar, Patricia Brophy, Thomas D. Green, Tonya N. Zeczycki, Feilim Mac Gabhann, Brian H. Annex, Joseph M. McClung
Summary: CLI is the most severe manifestation of PAD with higher prevalence in Black patients. Differences in bioenergetic paradigm and alterations in specific pathways were observed in the limb muscles of Black individuals with CLI.
Article
Hematology
Sarah B. Mueller, Paola Dal Cin, Long P. Le, Dora Dias-Santagata, Jochen K. Lennerz, A. John Iafrate, Hetal Desai Marble, Andrew M. Brunner, Matthew J. Weinstock, Marlise R. Luskin, Daniel J. De Angelo, Richard M. Stone, Valentina Nardi
Summary: This study identified non-PDGFRA gene fusions, such as ETV6-SCFD2, using sequencing technology in AML patients, contradicting the previous findings based on FISH analysis. These results highlight the importance of using sequencing-based assays to confirm the presence of targetable gene fusions in genomic regions.
Review
Biochemistry & Molecular Biology
Cameron A. Schmidt
Summary: Mitochondria play a central role in the physiology and survival of eukaryotic cells. Small molecules that modulate mitochondrial metabolism can have complex systemic effects. This article reviews the effects of prescription drugs on mitochondrial metabolism, discusses qualitative models of drug-mitochondria interactions, and highlights specific drug classes for further discussion.
BIOSCIENCE REPORTS
(2022)
Article
Developmental Biology
Paige DeBenedittis, Anish Karpurapu, Albert Henry, Michael C. Thomas, Timothy J. McCord, Kyla Brezitski, Anil Prasad, Caroline E. Baker, Yoshihiko Kobayashi, Svati H. Shah, Christopher D. Kontos, Purushothama Rao Tata, R. Thomas Lumbers, Ravi Karra
Summary: Heart regeneration requires interaction between multiple cell types for cardiomyocyte proliferation. Our study reveals the spatiotemporal coupling of cardiac endothelial cells (CECs) and cardiomyocytes (CMs) in the proliferation process, and identifies the essential role of VEGF-VEGFR2 signaling in this coupling. Additionally, we find that boosting CEC density with a virus enhances heart regeneration.
Article
Cardiac & Cardiovascular Systems
Hasan Abbas, Lindsey A. Olivere, Michael E. Padgett, Cameron A. Schmidt, Brian F. Gilmore, Timothy J. McCord, Kevin W. Southerland, Joseph M. McClung, Christopher D. Kontos
Summary: Skeletal muscle injury in peripheral artery disease (PAD) is not only attributed to vascular insufficiency, but also to muscle cell responses. Genetic ablation of Pax7(+) muscle progenitor cells (MPCs) in a hindlimb ischemia model led to inhibited muscle regeneration. Adipogenesis and fibrosis were observed in the ischemic limb, and inhibition of fibro/adipogenic progenitors (FAPs) decreased adipose but increased fibrosis. Patients with critical limb threatening ischemia also showed increased adipose and decreased satellite cells in more ischemic regions. These findings suggest that muscle regeneration could be a potential therapeutic target in PAD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Clinical Neurology
Cameron Schmidt, Arada Chaiyamoon, Juan J. Cardona, Mansour Mathkour, Tyler Scullen, Joe Iwanaga, James Kalyvas, Ana Carrera, Francisco Reina, Aaron S. Dumont, R. Shane Tubbs
Summary: This study examines the discrepancies in terminology regarding the pedicle, pars interarticularis, and isthmus of C2 vertebra and proposes new terminology based on anatomical study. The results show that C2 has no isthmus and a very short pars interarticularis. Therefore, it is suggested to use the more accurate term "pedicle" to describe the unique structure of C2 vertebra.
ACTA NEUROCHIRURGICA
(2023)
Article
Immunology
Lindsay T. Fourman, Sarah B. Mueller, Autumn Boutin, Isabel Zheng, Chelsea S. Pan, Marisa E. Gerard, Takara L. Stanley, Drucilla J. Roberts
Summary: The study found that placentas from HIV-exposed uninfected pregnancies demonstrated increased villous vascularity compared with the unexposed controls, in proportion to the severity of maternal immune dysfunction. Within the HIV-exposed uninfected group, lower CD4(+) T-cell count during pregnancy was associated with greater placental vascularity. However, there were no significant relationships observed between placental blood vessel count per villus and body mass index z-score or reactive airway disease among HIV-exposed uninfected individuals later in life.
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
(2021)
Article
Medicine, Research & Experimental
Terence E. Ryan, Dean J. Yamaguchi, Cameron A. Schmidt, Tonya N. Zeczycki, Saame Raza Shaikh, Patricia Brophy, Thomas D. Green, Michael D. Tarpey, Reema Karnekar, Emma J. Goldberg, Genevieve C. Sparagna, Maria J. Torres, Brian H. Annex, P. Darrell Neuter, Espen E. Spangenburg, Joseph M. McClung
Article
Cardiac & Cardiovascular Systems
Joseph M. McClung, Timothy J. McCord, Terence E. Ryan, Cameron A. Schmidt, Tom D. Green, Kevin W. Southerland, Jessica L. Reinardy, Sarah B. Mueller, Talaignair N. Venkatraman, Christopher D. Lascola, Sehoon Keum, Douglas A. Marchuk, Espen E. Spangenburg, Ayotunde Dokun, Brian H. Annex, Christopher D. Kontos