Article
Immunology
Weili Zhang, Siying Wu, Jin Deng, Quanfeng Liao, Ya Liu, Li Xiong, Ling Shu, Yu Yuan, Yuling Xiao, Ying Ma, Mei Kang, Dongdong Li, Yi Xie
Summary: The research investigated the changes in TAT before and after the adoption of TLA in the laboratory, revealing a significant reduction in TAT for CSF samples and improvements in TAT for other types of samples. Additionally, there was a significant decrease in the incidence of broth growth for pre-TLA compared to post-TLA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
James W. Snyder, Gina K. Thomson, Stacy Heckman, Kira Jamros, Sameh AbdelGhani, Kenneth S. Thomson
Summary: The study compared the performance of the current BD Kiestra (TM) TLA system and a fully automated research-use-only prototype, BD Kiestra (TM) IdentifA/SusceptA, in a clinical setting. Results showed high agreement between the two systems, with the automated prototype demonstrating potential advantages for clinical microbiology laboratories.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Laerke Boye Astrup, Karl Pedersen, Michael Farre
Summary: This study compares the accuracy of diagnoses on bovine mastitis made by veterinary clinics using conventional methods with diagnoses made by a reference laboratory using MALDI-TOF MS as a diagnostic tool. The findings show that a significant number of diagnoses made by veterinary clinics are incorrect, resulting in the potential misuse of antibiotics.
Article
Medicine, General & Internal
Jian Zhang, Zhizhong Liu, Ribing Chen, Qingwei Ma, Qian Lyu, Shuhui Fu, Yufei He, Zijie Xiao, Zhi Luo, Jianming Luo, Xingyu Wang, Xiangyi Liu, Peng An, Wei Sun
Summary: This study constructed a machine learning model based on MALDI-TOF mass spectrometry to screen for thalassaemia, achieving good classification performance and showing great potential for rapid screening in large populations.
ANNALS OF MEDICINE
(2022)
Article
Immunology
Aline Cuenod, Frederic Foucault, Valentin Pfluger, Adrian Egli
Summary: Accurate and timely identification of bacterial species is crucial in clinical diagnostics, and MALDI-TOF MS is a widely used method for this purpose. However, challenges in species identification persist due to incomplete databases, closely related species, and vaguely defined spectral quality. This study identified quality measures for MALDI-TOF MS, optimized spectral quality, and found that factors such as using formic acid, measuring young bacterial colonies, and frequent calibration can improve mass spectral quality. Additionally, differences in spectral quality were observed between different bacterial taxa, with optimal measurement conditions varying for each taxon.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Psychiatry
Kristina Krajcikova, Erika Semancikova, Katarina Zakutanska, Daria Kondrakhova, Jana Maslankova, Marek Stupak, Ivan Talian, Natalia Tomasovicova, Tatiana Kimakova, Vladimir Komanicky, Katarina Dubayova, Dagmar Breznoscakova, Eva Palova, Juraj Semancik, Vladimira Tomeckova
Summary: Spectroscopic methods have shown promising potential in detecting subtle neurobiological abnormalities in major depressive disorder. By analyzing tear fluid samples using various spectroscopic techniques, researchers were able to differentiate between subjects with MDD and healthy controls, suggesting these methods could be useful in clinical psychiatry for differential diagnosis and monitoring treatment effects.
JOURNAL OF PSYCHIATRIC RESEARCH
(2021)
Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Review
Cell Biology
Maureen Feucherolles, Gilles Frache
Summary: MALDI-TOF MS is widely used in microbiology for identification, but requires further upgrades. MSI is popular in life sciences and can detect biomolecules while showing their spatial distribution.
Article
Biochemistry & Molecular Biology
Chia-Ru Chung, Hsin-Yao Wang, Po-Han Chou, Li-Ching Wu, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study used an ensemble of multiple preprocessing methods to extract critical information from complicated mass spectrometry spectral data for identifying microorganisms and predicting antibiotic resistance. The ensemble method outperformed individual methods, achieving the highest accuracy on independent testing datasets. Important peaks related to antibiotic resistance could be detected, providing valuable information for further investigation of the resistance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Aurelie Morand, Mamadou Lamine Tall, Edmond Kuete Yimagou, Issa Isaac Ngom, Cheikh Ibrahima Lo, Florent Cornu, Michel Tsimaratos, Jean-Christophe Lagier, Anthony Levasseur, Didier Raoult, Pierre-Edouard Fournier
Summary: This study describes a new species Anaerococcus urinomassiliensis, isolated from the urine of a 17-year-old boy with autoimmune hepatitis and membranoproliferative glomerulonephritis. The strain exhibits specific genetic characteristics, is primarily of human origin, and belongs to the genus of anaerobic bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Meritxell Deulofeu, Eladia M. Pena-Mendez, Petr Vanhara, Josef Havel, Lukas Moran, Lukas Pecinka, Anna Bago-Mas, Enrique Verdu, Victoria Salvado, Pere Boadas-Vaello
Summary: Pathological pain can be classified into neuropathic pain and nociplastic pain. Currently, there is no standard method for diagnosing pathological pain subtypes, leading to a challenge for clinicians. By using multimolecular techniques and artificial intelligence, specific fingerprints for pathological pain subtypes can be obtained, which can aid in diagnosis.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Infectious Diseases
Eun-Jeong Yoon, Seok Hoon Jeong
Summary: MALDI-TOF MS has become a routine diagnostic process for infectious diseases, offering a fast, low-cost, and simple methodology in clinical microbiology laboratories. It can identify antimicrobial resistance through various approaches, such as comparing bacterial spectra and determining peak shifts in the presence of antimicrobial drugs. This technique shows promise for accurate and rapid identification of AMR in bacteria.
Article
Infectious Diseases
Ivana Regecova, Jana Vyrostkova, Frantisek Zigo, Gabriela Gregova, Mariana Kovacova
Summary: This study aims to determine the prevalence of methicillin-resistant Staphylococcus chromogenes in samples of sheep's milk and cheeses, with findings showing high levels of antimicrobial resistance in the identified isolates, including resistance to commonly used antibiotics such as penicillin and tetracycline. The study also revealed a significant proportion of multidrug-resistant isolates, indicating a pressing issue of antibiotic resistance in nonaureus staphylococci.
Review
Agriculture, Dairy & Animal Science
Daryna Kurban, Jean-Philippe Roy, Fidele Kabera, Annie Frechette, Maryse Michele Um, Ahmad Albaaj, Sam Rowe, Sandra Godden, Pamela R. F. Adkins, John R. Middleton, Marie-Lou Gauthier, Greg P. Keefe, Trevor J. DeVries, David F. Kelton, Paolo Moroni, Marcos Veiga Dos Santos, Herman W. Barkema, Simon Dufour
Summary: Innovations in veterinary medicine diagnostic methods, such as MALDI-TOF mass spectrometry, can help identify microorganisms causing bovine mastitis. However, not all microorganisms cultured from bovine milk samples are necessarily pathogenic. This study aimed to determine the diversity and proportion of microorganisms in healthy and mastitis-infected cow milk samples and identify the knowledge gaps regarding their impact on udder health.
Article
Multidisciplinary Sciences
Kristine Korzow Richter, Maria C. Codlin, Melina Seabrook, Christina Warinner
Summary: Collagen peptide mass fingerprinting by MALDI-TOF-MS, also known as ZooMS, is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. It enables rapid taxonomic identification of large bone assemblages and cultural heritage objects. However, there are limitations that need to be addressed for future advancements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Computer Science, Software Engineering
T. Baumgartl, M. Petzold, M. Wunderlich, M. Hohn, D. Archambault, M. Lieser, A. Dalpke, S. Scheithauer, M. Marschollek, V. M. Eichel, N. T. Mutters, T. Von Landesberger
Summary: The study introduces a novel visual analytics approach to support the analysis of transmission pathways during hospital pathogen outbreaks, demonstrating significant benefits in terms of efficiency, extended analysis intervals, and broader coverage of hospital wards. Feedback from twenty-five experts from seven German hospitals confirms that the solution brings substantial advantages in analyzing outbreaks.
IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS
(2021)
Article
Public, Environmental & Occupational Health
O. Assadian, M. Golling, C. M. Krueger, D. Leaper, N. T. Mutters, B. Roth, A. Kramer
Summary: The study examines the impact of the COVID-19 pandemic on surgical workflows and the development of protocols to protect patients and healthcare workers from infection. It highlights the importance of adapting and continually evaluating recommendations to continue surgical services during the pandemic.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Infectious Diseases
Diane Ashiru-Oredope, Susan Hopkins, Sagar Vasandani, Eno Umoh, Olaolu Oloyede, Andrea Nilsson, John Kinsman, Linda Elsert, Dominique L. Monnet
Summary: The survey found that healthcare workers generally have a higher knowledge of antibiotics and their use compared to antibiotic resistance. Most healthcare workers reported receiving information on avoiding unnecessary antibiotic use, but only a small percentage provided resources to patients. Fear of patient deterioration or complications was the most common reason for prescribing unnecessary antibiotics.
Article
Infectious Diseases
Fabian von Dehn, Nico T. Mutters, Vanessa M. Eichel, Uta Merle, Thorsten Brenner, Michael Nafe, Anja Sander, Martin Wolkewitz, Uwe Frank
Summary: This study investigated the effect of daily whole-body bathing using disposable washcloth wipes/caps impregnated with the antiseptic DDAC. The results showed a reduced incidence of central-venous-line-associated infections caused by Gram-positive bacteria in ICU patients undergoing DDAC bathing. There was also a trend towards reduction in catheter-related bloodstream infections and bloodstream infections. DDAC may be a promising agent for whole-body bathing of ICU patients.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Public, Environmental & Occupational Health
S. Gemein, R. Andrich, B. Christiansen, M. Decius, M. Exner, B. Hunsinger, E. Imenova, G. Kampf, T. Koburger-Janssen, K. Konrat, H. Martiny, M. Meckel, N. T. Mutters, F-A Pitten, S. Schulz, I Schwebke, J. Gebel
Summary: The study compared the efficacy of five 'sporicidal' surface disinfectants against C. difficile spores, with products A, C, and D showing good sporicidal effects in suspension and on surfaces, while products B and E demonstrated less effectiveness.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Microbiology
Dennis Nurjadi, Martin Scherrer, Uwe Frank, Nico T. Mutters, Alexandra Heininger, Isabel Spaeth, Vanessa M. Eichel, Jonas Jabs, Katja Probst, Carsten Mueller-Tidow, Juliane Brandt, Klaus Heeg, Sebastien Boutin
Summary: The hospital water environment can serve as a reservoir for multiward outbreaks, leading to acquisitions or transmissions of multidrug-resistant organisms in a hospital setting. Most Gram-negative bacteria have the ability to form biofilms and persist in hospital plumbing systems for extended periods. Eliminating these reservoirs is crucial to preventing further transmission, but proposed decontamination regimens, such as using acetic acid, can only suppress rather than fully eliminate the environmental reservoir. This study demonstrated that colonization with multidrug-resistant organisms can be acquired through showering in showers with contaminated water traps and drains.
MICROBIOLOGY SPECTRUM
(2021)
Article
Public, Environmental & Occupational Health
V. M. Eichel, S. Boutin, U. Frank, M. A. Weigand, A. Heininger, N. T. Mutters, M. W. Buechler, K. Heeg, D. Nurjadi
Summary: This study suggests that discontinuing contact precautions while enforcing basic hygiene measures does not increase the transmission of VREfm, indicating that it may be feasible in controlling VREfm infection.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Public, Environmental & Occupational Health
A. A. Mardiko, S. Heinemann, A. Bludau, H. E. J. Kaba, A. Leha, N. von Maltzahn, N. T. Mutters, R. Leistner, F. Mattner, S. Scheithauer
Summary: The vaccination strategy for healthcare workers in Germany varied across hospitals and deviated from the recommendations of the German standing committee on vaccination (STIKO). Further studies are needed to analyze the reasons for the low vaccination rate among healthcare workers and to adopt effective strategies.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Virology
Manuel Doehla, Bianca Schulte, Gero Wilbring, Beate Mareike Kuemmerer, Christin Doehla, Esther Sib, Enrico Richter, Patrick Frank Ottensmeyer, Alexandra Haag, Steffen Engelhart, Anna Maria Eis-Huebinger, Martin Exner, Nico Tom Mutters, Ricarda Maria Schmithausen, Hendrik Streeck
Summary: This study aimed to investigate the role of environmental transmission of SARS-CoV-2 in quarantined households. The results showed that out of the 43 tested adults, 26 tested positive using qRT-PCR. Air samples were negative, while 10 out of 66 wastewater samples and 4 out of 119 surface samples were positive for SARS-CoV-2. However, there was no significant correlation between qRT-PCR-positive environmental samples and the extent of infection spread among household members. No infectious virus could be propagated under cell culture conditions. Therefore, this study demonstrates a low likelihood of transmission via surfaces.
Article
Infectious Diseases
Mykhailo Savin, Gabriele Bierbaum, Nico T. Mutters, Ricarda Maria Schmithausen, Judith Kreyenschmidt, Isidro Garcia-Menino, Silvia Schmoger, Annemarie Kaesbohrer, Jens Andre Hammerl
Summary: This study characterizes carbapenem-resistant Klebsiella spp. in wastewater and surface water in Germany. The isolates showed resistance to multiple antibiotics and a high diversity of antibiotic-resistance genes. Virulence factors were also present in most isolates. The findings suggest the possible dissemination of resistant bacteria in the environment and the risk of colonization and infection in humans, livestock, and wildlife.
Article
Public, Environmental & Occupational Health
A. Bludau, S. Heinemann, A. A. Mardiko, H. E. J. Kaba, A. Leha, N. von Maltzahn, N. T. Mutters, R. Leistner, F. Mattner, S. Scheithauer
Summary: This study aimed to investigate the understanding of infection control practitioners in German hospitals regarding COVID-19 infection control strategies. The results showed that the decision to separate areas and teams seemed to depend on the hospital's structural conditions. Additionally, there were differences in accompanying regulations between hospitals of different sizes, which may be related to patient numbers, case type/severity, and patients' requirements.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Biotechnology & Applied Microbiology
Lia Freier, Nicole Zacharias, Stefanie Gemein, Juergen Gebel, Steffen Engelhart, Martin Exner, Nico T. Mutters
Summary: Wastewater pipes serve as microbial ecosystems, which can pose a potential infection risk. This study investigates the impact of C. difficile-infected patients on environmental contamination and identifies potential reservoirs. The findings highlight the importance of addressing the risk of transmission and infection from these reservoirs to ensure patient safety.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Amelia A. Mardiko, Anna Bludau, Stephanie Heinemann, Hani E. J. Kaba, Diana Fenz, Andreas Leha, Nicole von Maltzahn, Nico T. Mutters, Rasmus Leistner, Frauke Mattner, Simone Scheithauer
Summary: In order to prevent healthcare workers from getting infected with COVID-19 at work and ensure a safe environment in hospitals, comprehensive infection control strategies, including the use of personal protective equipment (PPE), regulations during breaks for healthcare workers, and the dissemination of pandemic-related information, are necessary.
Article
Environmental Sciences
Mykhailo Savin, Jens Andre Hammerl, Julia Hassa, Norman Hembach, Joern Kalinowski, Thomas Schwartz, Felix Droop, Nico T. Mutters
Summary: Wastewater treatment plants are major reservoirs for the spread of antibiotic-resistant bacteria and antibiotic resistance genes. This study highlights the importance of extracellular DNA in wastewater, which is often overlooked. The results suggest that depending on the origin and treatment methods of wastewater, extracellular DNA can serve as an important reservoir for antibiotic resistance genes.
ENVIRONMENTAL POLLUTION
(2023)
Article
Medicine, General & Internal
Timo Falkenberg, Felix Wasser, Nicole Zacharias, Nico Mutters, Thomas Kistemann
Summary: The aim of this study was to evaluate the effect of high-efficiency particulate air (HEPA) filters on COVID-19 prevalence in kindergartens. The results showed that the use of HEPA filters in kindergartens in Germany did not reduce the prevalence of COVID-19, as contagion mainly occurs through direct face-to-face contact and the contaminated air does not necessarily pass through the filter before being exchanged between children.
