Article
Agriculture, Dairy & Animal Science
S. M. Rowe, S. M. Godden, E. Royster, J. Timmerman, M. Boyle
Summary: This prospective cohort study explored the associations between intramammary infection in late-lactation cows and postcalving udder health and productivity. Late lactation IMI was found to increase the risk of clinical and subclinical mastitis in the subsequent lactation, leading to decreased milk yield. Different pathogens causing IMI may have varied effects on postcalving health and productivity.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Ecology
Jun-Hong Ch'ng, Mugil Muthu, Kelvin K. L. Chong, Jun Jie Wong, Casandra A. Z. Tan, Zachary J. S. Koh, Daniel Lopez, Artur Matysik, Zeus J. Nair, Timothy Barkham, Yulan Wang, Kimberly A. Kline
Summary: This study reports the enhanced biofilm biomass resulting from the combination of Staphylococcus aureus and Enterococcus faecalis, which is dependent on the activation of E. faecalis aerobic respiration. S. aureus provides heme to activate E. faecalis respiration, and E. faecalis gelatinase activity facilitates heme extraction. This interspecies interaction and metabolic cross-feeding may explain the frequent co-occurrence of these microbes in biofilm-associated infections.
Article
Orthopedics
Aron Sulovari, Mark J. Ninomiya, Christopher A. Beck, Benjamin F. Ricciardi, Constantinos Ketonis, Addisu Mesfin, Nathan B. Kaplan, Sandeep P. Soin, Susan M. McDowell, Bilal Mahmood, John L. Daiss, Edward M. Schwarz, Irvin Oh
Summary: The development of a multiplex immunoassay and enriched medium for diagnosing S. aureus and GBS in MSKI showed high diagnostic potential. The study findings support the development of species-specific immunoassays for identifying causal pathogens in active MSKI, especially in conjunction with standard culture.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Infectious Diseases
Etyene Schnurr, Pune N. Paque, Thomas Attin, Paolo Nanni, Jonas Grossmann, Silva Holtfreter, Barbara M. Broeker, Christian Kohler, Binh An Diep, Apoena de Aguiar Ribeiro, Thomas Thurnheer
Summary: In this study, researchers investigated the interaction between transient Staphylococcus aureus and other oral bacterial species, and found that the presence of S. aureus affected the distribution of other species in the biofilm differently. Deletion of MSCRAMM genes disrupted the growth of S. aureus and altered the distribution of S. mutans and S. oralis. S. aureus also played a role in structuring the biofilm architecture and transitioning from a homeostatic to a dysbiotic biofilm, contributing to the development of oral diseases.
Article
Medicine, General & Internal
Gregor Paul, Laurin Ochs, Christopher Hohmann, Stephan Baldus, Guido Michels, Charlotte Meyer-Schwickerath, Gerd Faetkenheuer, Navid Mader, Thorsten Wahlers, Carolyn Weber, Norma Jung
Summary: Staphylococcus aureus (SA) and Streptococcus species (SS) display different clinical manifestations in infective endocarditis (IE), with SA-IE patients having more adverse factors while SS-IE more commonly affects valve function.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Microbiology
Gen Li, Mark J. Walker, David M. P. De Oliveira
Summary: This review discusses the epidemiology of vancomycin-resistant Enterococcus and S. aureus in healthcare, community, and agricultural settings, explores vancomycin resistance in the context of van and non-van mediated resistance development, and provides insights into alternative therapeutic approaches aimed at treating drug-resistant Enterococcus and S. aureus infections.
Article
Food Science & Technology
Xinran Xiang, Jiaran Lu, Xiaowei Xu, Xiaogai Hou, Enjie Diao, Shiquan Qian, Huwei Song, Liqin Liang, Yinglong He, Yuting Shang
Summary: In this study, molecular targets specific to four Enterococcus species were screened and a PCR method was established for their rapid detection. The PCR method not only verified the specificity of the molecular targets, but also served as an effective tool for molecular diagnosis. The exploitation of these specific molecular targets and the unambiguous identification of Enterococcus species through PCR are important for determining food contamination and clinical diseases.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Luca Nunziata, Milena Brasca, Stefano Morandi, Tiziana Silvetti
Summary: Antibiotic resistance is a growing concern for public health and the global economy. Lactic acid bacteria (LAB) commonly found in dairy products and agro-zootechnical environments can serve as reservoirs for antibiotic resistance genes, which can be acquired or transferred to other microorganisms. This review focuses on LAB and Bifidobacterium species used in the dairy industry, analyzing data from the past 25 years to identify atypical resistance, genetic traits related to antibiotic resistance, and their potential to be transmitted to other microorganisms. Comparisons of resistomes were also conducted. The presence of antibiotic-resistant LAB in commercial dairy products has decreased due to worldwide restrictions on antibiotic use in animal husbandry, but transmissible resistances still exist in industrial cultures.
Article
Immunology
Emmanuel Chaumond, Sandrine Peron, Nathalie Daniel, Yann Le Gouar, Eric Guedon, David L. Williams, Yves Le Loir, Gwenal Jan, Nadia Berkova
Summary: This study demonstrates the development of innate immune memory in non-immune cells during Staphylococcus aureus infection and uncovers the epigenetic reprogramming mechanisms involved. These findings provide insight into the potential for new therapeutic approaches to prevent S.aureus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Chunyan Tan, Qiuyue Li, Xuejiao Yang, Jiayu Chen, Qilin Zhang, Xianyu Deng
Summary: Lactococcus lactis KUST48 (LLK48) with antibacterial effect against Streptococcus agalactiae was isolated and used as a potential probiotic to treat zebrafish infected with S. agalactiae. The study demonstrated the positive therapeutic effects of LLK48 on the intestinal microbiota and physiological functions of zebrafish infected with S. agalactiae, providing a new approach to treat S. agalactiae infections in fish aquaculture.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
A. Goer, L. S. Blanchard, A. Van Belkum, K. J. Loftus, T. P. Armstrong, S. G. Gatermann, D. Shortridge, B. J. Olson, J. K. Meece, T. R. Fritsche, M. Pompilio, D. Halimi, C. Franceschi
Summary: This study redeveloped and evaluated the fosfomycin strip, which showed good clinical performance against various pathogens, making it a potential substitute for the traditional method.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hossein Meghdadi, Azar Dokht Khosravi, Mohammad Hashemzadeh, Mohammad Reza Tabandeh
Summary: This study aimed to differentiate NTM clinical isolates by evaluating multiple genes and using MLSA technique to concatenate these genes. It was found that MLSA technique could identify all isolates to the species level.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemical Research Methods
Xinghong Zhao, Oscar P. Kuipers
Summary: This study demonstrates the synthesis of novel macrocyclic lanthipeptides using thanatin and rip-thanatin as templates, showing selective antimicrobial activity against S. aureus, including an antibiotic-resistant MRSA strain. The hybrid peptides of nisin(1-20) and ripcin, ripcin B-G, which are inactive against Gram-negative pathogens, exhibited substantial antimicrobial activity against tested Gram-negative pathogens and were highly resistant against the nisin resistance protein NSR.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Christian Salgard Jensen, Katrine Hojholt Iversen, Rimtas Dargis, Patricia Shewmaker, Simon Rasmussen, Jens Jorgen Christensen, Xiaohui Chen Nielsen
Summary: Different methods were used to identify Streptococcus pseudopneumoniae, with core genome analysis and CSI phylogeny proving effective in clustering strains accurately. However, single gene or MLSA methods could lead to misidentification. KmerFinder showed high accuracy in identifying S. pseudopneumoniae.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eugenia Butucel, Igori Balta, Iulia Adelina Bundurus, Cosmin Alin Popescu, Tiberiu Iancu, Adelina Venig, Ioan Pet, Ducu Stef, David McCleery, Lavinia Stef, Nicolae Corcionivoschi
Summary: This study investigated the effect of a natural antimicrobial mixture (Auraguard-Ag) on the infection of primary canine oral epithelial cells by Staphylococcus aureus, Streptococcus pyogenes, and Enterococcus faecalis. The results showed that a concentration of 0.25% Ag can inhibit the growth of these pathogens, while a concentration of 0.5% is bactericidal. A concentration of 0.125% Ag can significantly reduce biofilm formation and exopolysaccharide production, as well as the ability to infect oral epithelial cells.