4.7 Article

Polyelectrolyte Multilayer Coating of 3D Scaffolds Enhances Tissue Growth and Gene Delivery: Non-Invasive and Label-Free Assessment

期刊

ADVANCED HEALTHCARE MATERIALS
卷 3, 期 4, 页码 572-580

出版社

WILEY-BLACKWELL
DOI: 10.1002/adhm.201300301

关键词

tissue engineering; gene delivery; layer-by-layer deposition; dielectric impedance spectroscopy; phase microscopy

资金

  1. Le Fonds Quebecois de la Recherche sur la Nature et les Technologies
  2. National Science and Engineering Research Council of Canada
  3. Canadian Institutes of Health Research
  4. Research Councils UK
  5. EPSRC

向作者/读者索取更多资源

Layer-by-layer (LbL) deposition is a versatile technique which is beginning to be be explored for inductive tissue engineering applications. Here, it is demonstrated that a polyelectrolyte multilayer film system composed of glycol-chitosan (Glyc-CHI) and hyaluronic acid (HA) can be used to coat 3D micro-fabricated polymeric tissue engineering scaffolds. In order to overcome many of the limitations associated with conventional techniques for assessing cell growth and viability within 3D scaffolds, two novel, real-time, label-free techniques are introduced: impedance monitoring and optical coherence phase microscopy. Using these methods, it is shown that LbL-coated scaffolds support in vitro cell growth and viability for a period of at least two weeks at levels higher than uncoated controls. These polyelectrolyte multilayer coatings are then further adapted for non-viral gene delivery applications via incorporation of DNA carrier lipoplexes. Scaffold-based delivery of the enhanced green fluorescent protein (EGFP) marker gene from these coatings is successfully demonstrated in vitro, achieving a two-fold increase in transfection efficiency compared with control scaffolds. These results show the great potential of Glyc-CHI/HA polyelectrolyte multilayer films for a variety of gene delivery and inductive tissue engineering applications.

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