期刊
ACS SYNTHETIC BIOLOGY
卷 4, 期 1, 页码 32-38出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb500165g
关键词
continuous culture; turbidostat; characterization; fluorescence detection
资金
- National Science Foundation (NSF) [1317653]
- Paul G. Allen Family Foundation
- NSF [1120425]
- National Institute of General Medical Sciences from the National Institutes of Health [P41 GM103533]
- [T32 HG00035]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [T32HG000035] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103533] Funding Source: NIH RePORTER
- Direct For Biological Sciences [1120425] Funding Source: National Science Foundation
Engineered biological circuits are often disturbed by a variety of environmental factors. In batch culture, where the majority of synthetic circuit characterization occurs, environmental conditions vary as the culture matures. Turbidostats are powerful characterization tools that provide static culture environments; however, they are often expensive, especially when purchased in custom configurations, and are difficult to design and construct in a lab. Here, we present a low cost, open source multiplexed turbidostat that can be manufactured and used with minimal experience in electrical or software engineering. We demonstrate the utility of this system to profile synthetic circuit behavior in S. cerevisiae. We also demonstrate the flexibility of the design by showing that a fluorometer can be easily integrated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据