Article
Cell Biology
Lopez-Bailon Luis, Gonzalez-Telona Ana, Galan-Enriquez Carlos, Garcia-Gil Abraham, Estrada-Garcia Iris, Moreno-Lafont Martha, Ortiz-Navarrete Vianney
Summary: Following infection of B cells, Salmonella activates mTORC1 through SopB, inhibiting autophagy and promoting bacterial survival within the cells.
Article
Immunology
Bing-Xin Chu, Ya-Nan Li, Ning- Liu, Lan-Xin Yuan, Shi-Yan Chen, Yao-Hong Zhu, Jiu-Feng Wang
Summary: Salmonella Infantis inhibits apoptosis in infected cells by phosphorylating Akt, delaying pyroptosis and increasing severity of inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Lianxin Li, Jinge Xin, Hesong Wang, Yadong Wang, Weiqi Peng, Ning Sun, Haonan Huang, Yanxi Zhou, Xingmei Liu, Yu Lin, Jing Fang, Bo Jing, Kangcheng Pan, Yan Zeng, Dong Zeng, Xiang Qin, Yang Bai, Xueqin Ni
Summary: Fluoride is a common contaminant that poses challenges to animal and human health. This study investigates the role of the cytoskeleton in fluoride-induced barrier dysfunction and provides potential therapeutic targets for fluoride-induced intestinal injury.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Endocrinology & Metabolism
Xiaolin Chen, Jianhui Chen, Xianfan Li, Zengpu Yu
Summary: This study investigated the mechanisms of high glucose-induced glomerular filtration barrier dysfunction in human renal glomerular endothelial cells. The results showed that high glucose stimulation upregulated RhoA expression and activity through an mTOR-dependent pathway, leading to MLC-mediated endothelial cell cytoskeleton rearrangement and glomerular endothelial barrier dysfunction.
DIABETOLOGY & METABOLIC SYNDROME
(2021)
Review
Pharmacology & Pharmacy
Mahalaxmi Iyer, Mohana Devi Subramaniam, Dhivya Venkatesan, Ssang-Goo Cho, Matias Ryding, Morten Meyer, Balachandar Vellingiri
Summary: Parkinson's disease is a complex neurodegenerative disease characterized by depletion of midbrain dopaminergic neurons. Current challenges in PD research include finding therapies to stop or reverse disease progression, identifying reliable biomarkers, and improving understanding of the pathophysiological mechanisms. The RhoA-ROCK signaling pathway shows promise in potentially addressing PD symptoms and pathogenesis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Editorial Material
Cell Biology
Xiaofu Cao, Jeremy M. Baskin
Summary: This study reveals an unprecedented mechanism of synthesizing PtdIns(3,4)P-2 without the involvement of kinases and ATP through a phosphotransferase mechanism during bacterial infection.
NATURE CELL BIOLOGY
(2022)
Review
Behavioral Sciences
RuoLan Cai, YangYang Wang, ZhenTing Huang, Qian Zou, YinShuang Pu, Changyin Yu, Zhiyou Cai
Summary: ROCK activation can promote the occurrence of Alzheimer's disease and may involve a positive feedback loop between Aβ and ROCK. Additionally, ROCK can also promote the formation of neurofibrillary tangles and exacerbate neuroinflammatory responses.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shidong Shan, Min Su, Yan Li, Zhen Wang, Daoquan Liu, Yongying Zhou, Xun Fu, Shu Yang, Junchao Zhang, Jizhang Qiu, Huan Liu, Guang Zeng, Ping Chen, Xinghuan Wang, Michael E. Disanto, Yuming Guo, Xinhua Zhang
Summary: Our study demonstrated that RhoA regulates both static and dynamic factors of BPH. The RhoA-ROCK-beta-catenin signaling axis played a crucial role in the development of BPH and could provide new possibilities for subsequent clinical treatment strategies.
MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kinga Duszyc, Guillermo A. Gomez, Anne K. Lagendijk, Mei-Kwan Yau, Bageshri Naimish Nanavati, Briony L. Gliddon, Thomas E. Hall, Suzie Verma, Benjamin M. Hogan, Stuart M. Pitson, David P. Fairlie, Robert G. Parton, Alpha S. Yap
Summary: Extrusion is the primary response to apoptosis in epithelial cells, involving E-cadherin and RhoA signaling pathways. Mechanical tension-sensitive mechanotransduction activates RhoA specifically in neighboring cells of apoptotic cells, with S1P also playing a crucial role in the process.
Article
Medicine, Research & Experimental
Mohamed A. Saleh, Ahmed A. Shaaban, Iman M. Talaat, Atef Elmougy, Saryia F. Adra, Firdos Ahmad, Rizwan Qaisar, Adel B. Elmoselhi, Eman Abu-Gharbieh, Waseem El-Huneidi, Mohamed A. Eladl, George Shehatou, Hoda E. Kafl
Summary: This study investigated the potential role of ET-1 receptors in kidney diseases and found that ET-1 increases glomerular permeability and albuminuria by modulating the activity of the ETA-RhoA/ROCK-1/2 pathway. Selective ETA receptor blockade may represent a potential therapeutic strategy to limit glomerular injury and albuminuria.
Article
Oncology
Xiao Wei, Hua Lou, Dongchen Zhou, Yijuan Jia, Huayi Li, Quanfu Huang, Jingjing Ma, Zongyuan Yang, Chaoyang Sun, Yunchong Meng, Sen Xu, Xin Yang, Xiaoting Li, Teng Ji, Zhongzhen Guo, Qinglei Gao
Summary: This study confirmed that omental metastases in OC are stiffer and more abundant than primary tumors, and matrix stiffness can regulate OC progression. TAGLN was identified as a potent mechanosensitive gene that mediates stiffness-regulated OC progression through RhoA/ROCK pathway. Targeting ECM stiffness and TAGLN may provide promising therapeutic value for OC therapy.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Neurosciences
Abhishek Roy, Zarna Pathak, Hemant Kumar
Summary: The difficulties in regenerating after CNS injuries, including spinal cord injury, are attributed to factors such as hypertrophic scarring, inhibitory molecules, and the absence of mechanisms responsible for axonal regeneration in the adult CNS. Strategies to improve axon regeneration include neutralizing inhibitors/proteins and stabilizing microtubules. Furthermore, the focus on counteracting inhibitors of axonal growth and their downstream signaling through the RhoA/ROCK pathway is emphasized.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Cell Biology
Chen Qu, Wen Yang, Yating Kan, Hui Zuo, Mengqi Wu, Qing Zhang, Heng Wang, Dou Wang, Jiong Chen
Summary: Proper regulation of mitochondrial dynamics is critical for collective migration of Drosophila border cells during oogenesis. Misregulation of mitochondrial fission or fusion results in reduced ATP levels. RhoA/ROCK signaling may couple actomyosin dynamics with mitochondrial dynamics to achieve optimal actomyosin function during cell migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mahmoud Osman Khalifa, Takahito Moriwaki, Shouhua Zhang, Wei Zhou, Kosei Ito, Tao-Sheng Li
Summary: Biomechanical forces have the ability to regulate cell behaviors. This study investigated the role of negative pressure in regulating the dedifferentiation of hepatocytes. It was found that exposure to -50 mmHg pressure quickly induced stress fiber formation and changes in cell morphology. Furthermore, the activation of RhoA/ROCK pathway and the upregulation of stemness markers were observed. Inhibition of ROCK abrogated these changes induced by -50 mmHg pressure.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Weizhuo Lu, Zhiwu Chen, Jiyue Wen
Summary: Ischemic stroke is a leading cause of death and disability, and neuroinflammation plays a crucial role in the prognosis of stroke. The up-regulation of RhoA/ROCK pathway and the activation of NF-κB contribute to intense neuroinflammation and brain injury. Glial cells, including microglial cells and astrocytes, are deeply involved in the neuroinflammation cascade following ischemic stroke. Understanding the relationship among RhoA/ROCK pathway, NF-κB, and glial cells may offer new strategies for preventing intense neuroinflammation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Stephanie Stahnke, Hermann Doering, Charly Kusch, David J. J. de Gorter, Sebastian Duetting, Aleks Guledani, Irina Pleines, Michael Schnoor, Michael Sixt, Robert Geffers, Manfred Rohde, Mathias Muesken, Frieda Kage, Anika Steffen, Jan Faix, Bernhard Nieswandt, Klemens Rottner, Theresia E. B. Stradal
Summary: The genetic removal of Hem1 in macrophages leads to decreased phagocytosis and cell adhesion, as well as defects in cell migration. The study also found that the WRC impacts integrin-dependent processes in a FAK-dependent manner, controlling the formation and dismantling of adhesions during cell edge expansion.
Article
Cell Biology
Alexander Leithner, Lukas M. Altenburger, Robert Hauschild, Frank P. Assen, Klemens Rottner, Theresia E. B. Stradal, Alba Diz-Munoz, Jens Stein, Michael Sixt
Summary: Dendritic cells play a crucial role in the initiation of adaptive immunity by regulating the formation of immunological synapses with T cells through dynamic actin cytoskeletal rearrangements, demonstrating the importance of DC actin in immune synapse functionality.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Vanessa Dimchev, Ines Lahmann, Stefan A. Koestler, Frieda Kage, Georgi Dimchev, Anika Steffen, Theresia E. B. Stradal, Franz Vauti, Hans-Henning Arnold, Klemens Rottner
Summary: This study investigated the effects of acute Arp2/3 complex removal on the actin cytoskeleton regulation, showing both direct and indirect impacts on cell morphology, migration directionality, and chemotaxis. Depletion of Arp2/3 complex resulted in altered cell behavior and cytoskeletal dynamics, highlighting the complexities of actin network maintenance and cellular motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Klemens Rottner, Theresia E. B. Stradal
Summary: The study reveals that WASP plays a role in neutrophil migration by inducing localized actin assembly through the recruitment of Arp2/3 complex at substrate-induced membrane deformations near the cell front.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tak Shun Fung, Rajarshi Chakrabarti, Jana Kollasser, Klemens Rottner, Theresia E. B. Stradal, Frieda Kage, Henry N. Higgs
Summary: Mitochondrial damage triggers metabolic changes and mitophagy in cells. This study reveals the mechanism of a rapid peri-mitochondrial actin polymerization called acute damage-induced actin (ADA). The activation of ADA involves multiple signaling pathways and proteins, and it inhibits the rapid mitochondrial shape changes that occur upon mitochondrial depolarization.
Article
Cell Biology
Daniel Kummer, Tim Steinbacher, Sonja Tholmann, Mariel Flavia Schwietzer, Christian Hartmann, Simone Horenkamp, Sabrina Demuth, Swetha S. D. Peddibhotla, Frauke Brinkmann, Bjorn Kemper, Jurgen Schnekenburger, Matthias Brandt, Timo Betz, Ivan Liashkovich, Ivan U. Kouzel, Victor Shahin, Nathalie Corvaia, Klemens Rottner, Katsiaryna Tarbashevich, Erez Raz, Lilo Greune, M. Alexander Schmidt, Volker Gerke, Klaus Ebnet
Summary: This study identifies the cell adhesion molecule JAM-A as a central regulator of contact inhibition of locomotion (CIL) in tumor cells. JAM-A links to αvβ5 integrin through tetraspanins CD9 and CD81, and inhibits the activity of Src. Loss of JAM-A leads to increased motility, enhanced cell-matrix turnover, and failure to halt migration upon collision with other cells. Proper regulation of CIL also depends on αvβ5 integrin engagement.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Cell Biology
Jan Faix, Klemens Rottner
Summary: This review summarizes the molecular mechanisms of Ena/VASP-mediated actin filament assembly and discusses the cellular biological functions of Ena/VASP proteins in cell edge protrusion, migration, and adhesion.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Marco Kirchenwitz, Stephanie Stahnke, Silvia Prettin, Malgorzata Borowiak, Laura Menke, Christian Sieben, Carmen Birchmeier, Klemens Rottner, Theresia E. B. Stradal, Anika Steffen
Summary: SMER28 is a compound that induces autophagy through an unknown mechanism. It also has additional effects such as growth retardation and inhibition of receptor tyrosine kinase signaling, which leads to the inhibition of cell scattering and dorsal ruffle formation. Furthermore, SMER28 interferes with the entry of Listeria monocytogenes by inhibiting the signaling of the prominent receptor tyrosine kinase c-Met. In B cell lymphoma cells, which rely on PI3Kδ signaling, SMER28 induces apoptosis.
Article
Biochemistry & Molecular Biology
Nathaniel L. Lartey, Hilda Vargas-Robles, Idaira M. Guerrero-Fonseca, Alexander Garcia-Ponce, Citlaltepetl Salinas-Lara, Klemens Rottner, Michael Schnoor
Summary: Cortactin plays a crucial role in regulating neutrophil trafficking and severity of sepsis-induced lung injury.
Review
Cell Biology
Frieda Kage, Hermann Doering, Magdalena Mietkowska, Matthias Schaks, Franziska Gruner, Stephanie Stahnke, Anika Steffen, Mathias Muesken, Theresia E. B. Stradal, Klemens Rottner
Summary: A signaling pathway has been discovered that promotes Arp2/3 complex-dependent actin remodeling without the need for WRC and Ena/VASP, through Rac and Cdc42 GTPases.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Bojian Ding, Sheng Yang, Matthias Schaks, Yijun Liu, Abbigale J. Brown, Klemens Rottner, Saikat Chowdhury, Baoyu Chen
Summary: In this study, the structures of WRC, both alone and bound to Rac1, were investigated using cryogenic-electron microscopy. The findings reveal that Rac1 binds to WRC at two distinct sites, and binding to the A site activates the complex. Activation involves conformational changes that result in the release of sequestered WCA polypeptide, stimulating actin polymerization by the Arp2/3 complex. These structural insights, combined with biochemical and cellular analyses, provide a novel understanding of the regulation of the Rac1-WRC-Arp2/3-actin signaling axis in various biological processes and diseases.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Irshad Sharafutdinov, Jakob Knorr, Klemens Rottner, Steffen Backert, Nicole Tegtmeyer
Summary: Pathogenic bacteria pose a serious threat to human and animal health, and the dynamics of actin cytoskeleton play a crucial role in host-pathogen interactions. Cortactin, a major cellular factor, is involved in the regulation of these interactions, and various microbes have been reported to exploit cortactin signaling during infection.
MOLECULAR MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Thomas Pokrant, Jens Ingo Hein, Sarah Koerber, Andrea Disanza, Andreas Pich, Giorgio Scita, Klemens Rottner, Jan Faix
Summary: Lamellipodia, actin-based sheet-like membrane protrusions, drive 2D-cell migration, while microspikes are actin-filament bundles embedded within lamellipodia. Ena/VASP proteins and MyoX are found to be essential for microspike formation and their tight functional association in lamellipodia.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Florian Fassler, Manjunath G. Javoor, Julia Datler, Hermann Doring, Florian W. Hofer, Georgi Dimchev, Victor-Valentin Hodirnau, Jan Faix, Klemens Rottner, Florian K. M. Schur
Summary: Regulation of the Arp2/3 complex plays a crucial role in the nucleation of branched actin networks. Incorporation of subunit isoforms, such as ArpC5 and ArpC5L, fine-tunes nucleation activity and branch junction stability, affecting cell migration. Furthermore, ArpC5 isoforms impact the positioning of Ena/VASP proteins, which mediate isoform-specific effects on actin assembly and contribute to a signaling pathway enhancing cell migration.
Article
Cell Biology
Ramon Castellanos-Martinez, Iliana I. Leon-Vega, Idaira M. Guerrero-Fonseca, Hilda Vargas-Robles, Karina E. Jimenez-Camacho, Gabriela Hernandez-Galicia, Vianney F. Ortiz-Navarrete, Klemens Rottner, Oscar Medina-Contreras, Michael Schnoor
Summary: This article investigates the role of cortactin in T cell activation and migration in T cell acute lymphoblastic leukemia (T-ALL), and its significance in T-ALL development. The study demonstrates that cortactin participates in immune synapse formation and migration in normal T cells, while its overexpression in leukemic T cells leads to organ infiltration. Therefore, cortactin could be a potential therapeutic target for T-ALL and other pathologies involving aberrant T cell responses.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)