Review
Biochemistry & Molecular Biology
Parviz Vahedi, Rana Moghaddamshahabi, Thomas J. Webster, Ayse Ceren Calikoglu Koyuncu, Elham Ahmadian, Wasim S. Khan, Ali Jimale Mohamed, Aziz Eftekhari
Summary: Cartilage damage is often difficult to repair, and current treatment strategies are inadequate. Autologous mesenchymal stem cells are considered a promising therapeutic approach for cartilage regeneration, as they possess robust cartilaginous production capacity and are easy to isolate and expand without causing morbidity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Xiao Xu, Limei Xu, Jiang Xia, Caining Wen, Yujie Liang, Yuanmin Zhang
Summary: Osteoarthritis (OA) is a common disease characterized by cartilage degeneration in middle-aged and elderly people. Currently, there is no effective treatment for OA apart from total joint replacement in advanced stages. Mesenchymal stem cells (MSCs) have shown great potential in regulating the cartilage microenvironment, promoting cartilage regeneration, and alleviating OA symptoms, making them a promising source of cells for OA therapy.
ACTA BIOMATERIALIA
(2023)
Article
Medicine, General & Internal
Marco Caforio, Carmelo Nobile
Summary: The study found that the intra-articular administration of autologous purified adipose tissue following arthroscopy significantly reduces pain and improves functional recovery in patients with knee osteoarthritis. No adverse effects were observed during the 1-year follow-up.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jakub Kaszynski, Pawel Bakowski, Bartosz Kiedrowski, Lukasz Stolowski, Anna Wasilewska-Burczyk, Kamilla Grzywacz, Tomasz Piontek
Summary: This study evaluated the efficacy of PRP and AAT injections as a treatment for knee osteoarthritis. The results showed that both PRP and AAT injections significantly improved pain, symptoms, quality of life, and functional capacity in patients with mild to moderate knee osteoarthritis.
Review
Cell Biology
Yi Fan, Chen Cui, Peiran Li, Ruiye Bi, Ping Lyu, Yanxi Li, Songsong Zhu
Summary: Research has identified a novel stem cell niche in condylar cartilage, containing fibrocartilage stem cells (FCSCs) that play a crucial role in the development and regeneration of fibrocartilage. Understanding the properties of FCSCs may inform the development of promising biological strategies for the treatment of TMD in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Xiaowei Zhang, Shili Wu, Yong Zhu, Cong-Qiu Chu
Summary: The lack of effective treatment options for osteoarthritis is mainly due to the limited regenerative capacity of articular cartilage. Mesenchymal stem cells (MSCs) have drawbacks in current in vitro expanded approaches, while chondrocyte progenitors and MSCs in OA joints lack the activity of chondrogenic transcription factor SOX9. Supplementing with exogenous SOX9 may help activate the potential of these stem cells for cartilage regeneration.
FRONTIERS IN MEDICINE
(2021)
Article
Health Care Sciences & Services
Yang Yu, Qunshan Lu, Songlin Li, Mingxing Liu, Houyi Sun, Lei Li, Kaifei Han, Peilai Liu
Summary: This study examines the efficacy and safety of autologous micro-fragmented adipose tissue (MF-AT) in improving joint function and cartilage repair in knee osteoarthritis patients. The results show improvements in WOMAC, VAS, KSS, and HSS scores at 3, 6, 9, 12, and 18 months after injection compared to before injection. There were no adverse events reported. However, the improved knee function was not sustained, with the best results occurring at 9-12 months post-injection.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell Biology
Isabele Camargo Brindo da Cruz, Ana Paula Pereira Velosa, Solange Carrasco, Antonio dos Santos Filho, Jurandir Tomaz de Miranda, Eduardo Pompeu, Tiago Lazzaretti Fernandes, Daniela Franco Bueno, Camila Fanelli, Claudia Goldenstein-Schainberg, Alexandre Todorovic Fabro, Ricardo Fuller, Pedro Leme Silva, Vera Luiza Capelozzi, Walcy Rosolia Teodoro
Summary: The study found that ADSC stimulated by Col V can significantly increase Col II in the degenerative area of OA rabbits, regulating the expression of cartilage-related genes, making it a promising target for OA treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Lang Bai, Qibin Han, Zeyu Han, Xiaoyu Zhang, Jingwen Zhao, Huitong Ruan, Junliang Wang, Feng Lin, Wenguo Cui, Xing Yang, Yuefeng Hao
Summary: By utilizing chitosan-based cell adhesion, a bioadhesive porous microsphere is developed as a cell carrier for accelerating articular cartilage regeneration. After injection into the joint cavity, the microspheres expedite the release of mesenchymal stem cells (MSCs) in the microenvironment of arthritis inflammation, while using kartogenin to promote the differentiation of MSCs into chondrocytes, thereby accelerating articular cartilage regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Dawid Szwedowski, Joanna Szczepanek, Lukasz Paczesny, Jan Zabrzynski, Maciej Gagat, Ali Mobasheri, Slawomir Jeka
Summary: Knee osteoarthritis (KOA) treatment options include medication, physical therapy, braces, activity modification, and surgery, with intra-articular injections commonly used when non-operative treatment fails. PRP may improve the metabolic function of damaged structures, but its effects on chondrogenesis and stem cell proliferation are still controversial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Nesrine Ebrahim, Arigue A. Dessouky, Ola Mostafa, Amira Hassouna, Mohamed M. Yousef, Yasmin Seleem, Eman Abd El Aziz M. El Gebaly, Mona M. Allam, Ayman Samir Farid, Bayan A. Saffaf, Dina Sabry, Ahmed Nawar, Ahmed A. Shoulah, Ahmed H. Khalil, Sami F. Abdalla, Mohamed El-Sherbiny, Nehal M. Elsherbiny, Rabab F. Salim
Summary: The combined therapy of PRP and ADSCs significantly accelerated wound healing in experimentally induced diabetic rats by modulating the Notch signaling pathway, promoting angiogenesis, and enhancing EPSC proliferation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Orthopedics
Sara Sadat Nabavizadeh, Tahereh Talaei-Khozani, Moein Zarei, Shahrokh Zare, Omid Koohi Hosseinabadi, Nader Tanideh, Sajad Daneshi
Summary: The study evaluated the effects of SMMSCs, PRP, and secretome on collagenase II-induced rat knee osteoarthritis. Results showed that the combination of SMMSCs/secretome/PRP had a significant effect on glycosaminoglycans and collagen II contents, indicating better therapeutic efficacy.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Orthopedics
Andrea Pintore, Donato Notarfrancesco, Arnaldo Zara, Antonio Oliviero, Filippo Migliorini, Francesco Oliva, Nicola Maffulli
Summary: The study found that autologous mesenchymal stem cell (MSC) injections can improve clinical and functional outcomes in knee osteoarthritis (KOA) patients, with similar results between autologous bone marrow cells (BMAC) and adipose-derived stromal cells (ADSCs). Measurements of Knee Injury and Osteoarthritis Outcome Score (KOOS), Oxford Knee Score (OKS), and visual analog scale (VAS) were collected from 102 patients with KOA before the procedures, at one month, and at six months post-injection.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jai-Hong Cheng, Chieh-Cheng Hsu, Shan-Ling Hsu, Wen-Yi Chou, Yi-No Wu, Chun-En Aurea Kuo, Tsai-Chin Hsu, Li-Yen Shiu, Shun-Wun Jhan
Summary: The study showed that ADSC-CM and SW therapy can effectively reduce inflammatory cytokines, decrease inflammation-induced BMPs, and protect the articular cartilage of the OA joint.
Article
Cell & Tissue Engineering
R. Ossendorff, S. G. Walter, F. A. Schildberg, M. Khoury, G. M. Salzmann
Summary: Knee joint osteoarthritis is a complex immunological and degenerative disease, and current treatment strategies are ineffective in altering its progression. Mesenchymal stromal cell (MSC) therapy, which aims to regenerate the affected knee joint structures, has been researched for over 30 years. The secretome of MSCs has been found to play a central role in their regenerative capacity. Animal models have demonstrated the symptom- and disease-modifying effects of MSC therapy. Clinical studies have shown significant improvement in symptoms and functional outcomes with autologous and allogeneic MSC therapy, although contradictory results exist in the literature. The effectiveness of MSC therapy is still unclear and further research is needed.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Chemistry, Applied
Mai Thanh Thi Nguyen, Phu Hoang Dang, Hai Xuan Nguyen, Tho Huu Le, Truong Nhat Van Do, Phuc Van Pham, Sinh Truong Nguyen, Nhan Trung Nguyen
Summary: A new acridone alkaloid named paratrimerin I was isolated from the roots of Paramignya trimerawas through bioactivity-guided fractionation, and showed significant cytotoxicity against HepG2 and MCF-7 cell lines. The N-methyl, C-4 methoxy, and C-5 hydroxy groups in the acridone skeleton were suggested to contribute to its cytotoxic activity.
NATURAL PRODUCT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Thanh Minh Dang, Trinh Van Le, Huy Quang Do, Van Thuan Nguyen, Ai Xuan Le Holterman, Loan Tung Thi Dang, Nhan Chinh Lu Phan, Phuc Van Pham, Son Nghia Hoang, Long Thanh Le, Gabriele Grassi, Nhung Hai Truong
Summary: This study investigated the optimal conditions for HSC isolation and culture to maintain the undifferentiated phenotype. Nycodenz at 9.6% was found to yield the best purity and quantity of HSCs. Serum-free DMEM supplemented with glucose, GLN, vitA, and insulin allowed for the perpetuation of HSCs in culture while maintaining their undifferentiated state.
BIOSCIENCE REPORTS
(2021)
Article
Medicine, Research & Experimental
Thuan Minh Le, Giang Thi Dang, Huynh Duc Phat, Vu Bich Ngoc
Summary: The transplantation of Fi-MSC sheets positively impacted acute limb ischemia mouse models by improving SpO2 levels and enhancing blood reperfusion. Compared to the control group, the treatment group displayed better blood reperfusion, neovascularization, and muscle tissue repair, suggesting the potential efficacy of Fi-MSC sheet transplantation in treating acute limb ischemia.
BIOMEDICAL RESEARCH AND THERAPY
(2021)
Article
Gastroenterology & Hepatology
Trinh Van Le, Ngoc Bao Thi Dinh, Minh Thanh Dang, Nhan Chinh Lu Phan, Loan Tung Thi Dang, Gabriele Grassi, Ai Xuan Le Holterman, Huy Minh Le, Nhung Hai Truong
Summary: Chloroquine attenuates acute liver damage induced by CCl4 in mice by reducing liver enlargement and necrosis, as well as inhibiting HSC activation and autophagy. This protective effect may provide a potential novel therapeutic approach for hepatic fibrosis.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Review
Medicine, Research & Experimental
Hieu Trong Ngo, Phuc Van Pham
Summary: Breast cancer is the most common cancer in women worldwide, and conventional therapies often provide limited results, leading to the consideration of immunotherapy as a fourth-line treatment. The combination of CIKs and DCs in current clinical trials has shown significant efficacy, suggesting that CIK-DC therapy may soon emerge as a new option for breast cancer treatment.
BIOMEDICAL RESEARCH AND THERAPY
(2021)
Article
Cell Biology
Hieu Trong Ngo, Vy Thanh Dang, Nguyen Ho-Thao Nguyen, Anh Nguyen-Tu Bui, Phuc Van Pham
Summary: The study evaluates the anti-tumor potency of freshly thawed cytokine-induced killer cells (CIKs) compared to freshly cultured CIKs for cancer treatment. The results demonstrate that freshly thawed CIKs preserve their anti-tumor potency both in vitro and in vivo conditions, revealing the potential of off-the-shelf CIK products for cancer treatment.
CELL AND TISSUE BANKING
(2023)
Review
Medicine, Research & Experimental
Nhu Ngoc-Quynh Dinh, Ngoc Bich Vu, Phuc Van Pham
Summary: MSC transplantation shows potential in treating and improving post-COVID-19 pulmonary fibrosis, due to its immune modulation, anti-inflammatory, and anti-fibrotic properties.
BIOMEDICAL RESEARCH AND THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Nhat-Chau Truong, Nhi-Thao Huynh, Khuong-Duy Pham, Phuc-Van Pham
Summary: The interaction between cancer cells and the immune system has been extensively studied for over 130 years, but how to strengthen the immune system and suppress cancer cells remains unclear. Cancer stem cells, a subpopulation of cancer cells, have been found to play important roles in the initiation, progression, metastasis, and recurrence of various types of cancer. Despite the discovery of mechanisms by which cancer cells can escape immune surveillance, the specific roles of cancer stem cells in these mechanisms are still unknown. This review summarizes the mechanisms involving immune-related molecules in cancer cells and the roles of cancer stem cells in immune surveillance.
MINERVA BIOTECHNOLOGY AND BIOMOLECULAR RESEARCH
(2023)
Review
Endocrinology & Metabolism
Van Thi-Tuong Nguyen, Vuong Van Vu, Phuc Van Pham
Summary: Brown adipocytes have the potential for therapeutic applications in reversing metabolic conditions such as obesity and diabetes. They can produce heat through thermogenesis, which promotes balanced energy expenditure and improves metabolic disorders. The regulation of thermogenesis involves not only thermogenin UCPs within the mitochondria, but also signaling molecules that affect nearby and distant tissues. This review highlights the importance of understanding the crosstalk between different organelles within brown adipocytes and the interorgan axes in regulating whole body metabolism, and suggests considering the individual characteristics of brown adipocytes in future clinical applications.
Article
Medicine, Research & Experimental
Trinh Hoang-Nhat Nguyen, Phuc Van Pham, Ngoc Bich Vu
Summary: The efficacy of adipose stem cell-derived exosomes injection in treating acute mouse hindlimb ischemia was evaluated. The results showed that adipose stem cell-derived exosomes can promote angiogenesis and significantly reduce the rate of limb necrosis.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Thuan M. Le, Ngoc B. Vu, Phat T. Nguyen, Phat D. Huynh, Phuc Van Pham
Summary: This study evaluated the safety and efficacy of exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSC-exosomes) in treating acute hindlimb ischemia (HLI) in mouse models. The results showed that hUCMSC-exosomes had positive effects in treating HLI, including reducing limb injury, improving oxygen saturation level, promoting angiogenesis, and accelerating muscle tissue structure recovery.
MINERVA BIOTECHNOLOGY AND BIOMOLECULAR RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Viet Q. Pham, Nhan N. Tran, Binh T. Vu, Hanh T. Le, Ngoc B. Vu, Phuc Pham
Summary: The study found that preconditioning with hypoxia significantly increased the secretion of bFGF, VEGF, and HGF by both AT-MSCs and UC-MSCs, along with a significant up-regulation of related gene expression. In a quail embryo model, normoxic UC-MSCs showed better angiogenic promotion than normoxic AT-MSCs, but there was no significant difference in the angiogenic potency of hypoxic AT-MSCs and UC-MSCs.
MINERVA BIOTECHNOLOGY AND BIOMOLECULAR RESEARCH
(2021)
Review
Medicine, General & Internal
Ngoc B. Vu, Hoa T. Nguyen, Rosanna Palumbo, Rinaldo Pellicano, Sharmila Fagoonee, Phuc Pham
Summary: Stem cell therapy plays a significant role in the healing of chronic wounds, however, it is complex, expensive, and time-consuming. Recent studies suggest that stem cell-derived exosomes can be an effective treatment for wounds, inducing positive effects in various stages of wound healing.
Article
Biochemistry & Molecular Biology
Ha Thi-Ngan Le, Ngoc Bich Vu, Phuc Dang-Ngoc Nguyen, Thuy Thi-Thanh Dao, Xuan Hoang-Viet To, Phuc Van Pham
Summary: This study proposed a new method to produce cartilage tissues in vitro using adipose-derived stem cells in spheroids on porous scaffolds, showing promising results in rat models by demonstrating successful chondrogenic differentiation and maturation into cartilage tissues.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
