Article
Immunology
Mudra Khare, Dhiraj Sinha, Rita Zgheib, Sory Ibrahima Traore, Jean-Christophe Lagier, Didier Raoult, Pierre-Edouard Fournier
Summary: Strain Marseille-P1302, isolated from a 2-year-old Nigerian boy suffering from Kwash-iorkor, exhibits similarity with Brevilactibacter species, but is phylogenetically closest to Brevilactibacter sinopodophylli. Based on genomic data, a new genus Nigeribacterium is proposed, with strain Marseille-P1302 as the type strain of the new species Nigeribacterium massiliense gen. nov., sp. nov.
MICROBES AND INFECTION
(2021)
Article
Microbiology
Camille Valles, Morgane Mailhe, Davide Ricaboni, Nicholas Armstrong, Stephane Alibar, Veronique Vitton, Jean-Christophe Lagier, Didier Raoult, Maryam Tidjani Alou
Summary: This study describes the isolation of a new genus, Negativibacillus massiliensis, from the left colon of a healthy woman, with details on its characteristics and genomic information. The proposed creation of Negativibacillus massiliensis as the 43rd genus within the Ruminococcaceae family is based on the strain's genetic and phenotypic characteristics.
MICROBIOLOGY RESEARCH
(2021)
Article
Microbiology
Mossaab Maaloum, Cheikh Ibrahima Lo, Sokhna Ndongo, Marine Makoa Meng, Rachid Saile, Stephane Alibar, Didier Raoult, Pierre-Edouard Fournier
Summary: The culturomics method was used to isolate a new member of the Ottowia genus from a stool sample of a healthy volunteer. The isolated strain Marseille-P4747(T) showed a 96.18% 16S rRNA sequence identity with Ottowia beijingensis strain GCS-AN-3 (NR_133803.1), the closest named species. Based on various analysis, we concluded that Ottowia massiliensis sp. nov. is a new species represented by Marseille-P4747(T) ( = CSUR P4747 = CECT 30348), with a taxonogenomic description provided.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Microbiology
Sara Bellali, Gabriel Haddad, Thi-Phuong-Thao Pham, Rim Iwaza, Ahmad Ibrahim, Nicholas Armstrong, Amael Fadlane, Carine Couderc, Aldiouma Diallo, Cheikh Sokhna, Matthieu Million, Didier Raoult, Maryam Tidjani Alou
Summary: Two new bacterial strains were isolated from a 14-week-old Senegalese girl, classified into different families and orders, with genomes containing a high percentage of genes encoding enzymes related to genetic information processing, indicating a high growth rate and adaptability. These new taxa were extensively described and characterized using taxono-genomic description concept in this paper.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Maxime Descartes Mbogning Fonkou, Cheikh Ibrahima Lo, Zouina Mekhalif, Melhem Bilen, Enora Tomei, Edmond Kuete Yimagou, Gregory Dubourg, Didier Raoult, Florence Fenollar, Pierre-Edouard Fournier
Summary: Culturomics has enhanced research on human microbiota by isolating previously uncultured bacterial species. A new bacterial species, Gemella massiliensis, was successfully isolated from a sputum sample of a healthy French man. This strain exhibits unique characteristics and genetic differences compared to its closest known species, Gemella bergeri.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Mariem Ben Khedher, Fatou Samba Diouf, Cheikh Ibrahima Lo, Stephane Alibar, Guillaume Durand, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Summary: Strain GD8 is a new species isolated from fresh stool of a French volunteer, belonging to the order Coriobacteriales. It is an anaerobic Gram-positive bacterium isolated from human gut microbiota, with a genome size of 2,836,446 bp and a G + C content of 64.1%. The analysis of the 16S rRNA gene showed that GD8 has 96.2% similarity with Collinsella massiliensis strain An5, which is the phylogenetically related species.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Reham Magdy Wasfy, Malak Zoaiter, Melhem Bilen, Maryam Tidjani Alou, Cheikh Ibrahima Lo, Sara Bellali, Aurelia Caputo, Stephane Alibar, Claudia Andrieu, Didier Raoult, Pierre Edouard Fournier, Matthieu Million
Summary: This article reports a novel bacterial strain Marseille-P4005(T) isolated from a stool sample of a healthy donor. It is a Gram-stain negative, non-motile, non-spore-forming rod that grows optimally at 37 degrees C and at pH 7.0. Genomic analysis revealed it as a new species within the genus Agathobaculum, named Agathobaculum massiliense sp. nov. The strain showed resistance to several antibiotics and exhibited specific metabolic capabilities.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
He Qi, Defeng Liu, Yang Zou, Nan Wang, Han Tian, Chunling Xiao
Summary: C17(T) is a novel species of Streptococcus, isolated from a healthy child in Shenyang, China, with specific phenotypic and genomic characteristics, showing high susceptibility to several antibiotics.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Hoang Thong Kieu, Thi Phuong Thao Pham, Cheikh Ibrahima Lo, Stephane Alibar, Ludivine Brechard, Nicholas Armstrong, Philippe Decloquement, Aldiouma Diallo, Cheikh Sokhna, Matthieu Million, Jean-Christophe Lagier, Didier Raoult, Maryam Tidjani Alou
Summary: Using the culturomics approach, a previously unknown strain named Weizmannia faecalis was isolated and identified within the Weizmannia genus. The strain was isolated from a healthy subject's feces and showed similar characteristics to Weizmannia coagulans. However, there were some genomic differences between the two strains.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Mariema Sarr, Mamadou L. Tall, Mariem Ben Khedher, Thi-Phuong-Thao Pham, Babacar Mbaye, Aminata Camara, Nicholas Armstrong, Celine Chartier, Amael Fadlane, Cheikh Sokhna, Didier Raoult, Maryam Tidjani Alou, Matthieu Million
Summary: Two bacterial strains were isolated and identified, and a new genus and species, Konateibacter massiliensis gen. nov., sp. nov., and a new species, Paenibacillus faecalis sp. nov., were proposed based on their characteristics and genomic comparison.
CURRENT MICROBIOLOGY
(2022)
Article
Microbiology
Mossaab Maaloum, Pamela Afouda, Cheikh Ibrahima Lo, Gregory Dubourg, Thi Tien Nguyen, Anthony Levasseur, Rachid Saile, Didier Raoult, Pierre-Edouard Fournier
Summary: In this study, a new Prevotella species, Prevotella merdaesp. nov., isolated from humans was described. The bacterium was isolated from a fecal sample of a healthy fecal transplant donor. It displayed distinct phenotypic characteristics and had a unique genome composition.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Microbiology
Luis Johnson Kangale, Didier Raoult, Fournier Pierre-Edouard
Summary: A new bacterial strain, Marseille-Q2390, from the planarian S. mediterranea was isolated and characterized, leading to the proposal of a new species, Pedobacter ghigonii sp. nov. This white-pigmented, Gram-negative, rod-shaped bacterium grows in aerobic conditions and belongs to the family Sphingobacteriaceae. Genomic analysis confirmed its distinctiveness from closely related strains and its classification as a new species.
MICROBIOLOGY RESEARCH
(2021)
Article
Microbiology
Sory Ibrahima Traore, Cheikh Ibrahima Lo, Melhem Bilen, Didier Raoult, Florence Fenollar, Pierre-Edouard Fournier
Summary: Strain Marseille-P2012(T) has been identified as a new bacterial genus within the phylum Firmicutes, isolated from stool samples of a healthy 2-year-old Senegalese boy. This Gram-positive, anaerobic, non-motile coccus-shaped bacterium shows genetic similarity to Finegoldia magna strain ATCC 29328. Its genome is characterized by a length of 1,832,315 bp and a G + C content of 32.46 mol%.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Angeline Antezack, Manon Boxberger, Bernard La Scola, Virginie Monnet-Corti
Summary: This study described a novel species of Catonella, named Catonella massiliensis sp. nov., isolated from the saliva of a clinically healthy individual following successful treatment of periodontitis. The bacterium exhibited specific growth conditions and fatty acid profiles, showing genetic similarity to C. morbi but distinct enough to be classified as a new species within the genus.
Article
Microbiology
Wenbo Yang, Hui Yang, Xiaocun Bao, Mehboob Hussain, Qiang Bao, Zexuan Zeng, Chun Xiao, Lingyun Zhou, Xiaoping Qin
Summary: This study isolated a strain of Br. brevis HNCS-1 with antagonistic effect against tea disease pathogens, and identified edeine as the main antibacterial peptide of Br. brevis. Comparative genomics analysis revealed a shared core genome among Br. brevis strains, with only one NRPS gene cluster found.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Emilie Javelle, Aurelie Mayet, Rodrigue S. Allodji, Catherine Marimoutou, Chrystel Lavagna, Jerome Desplans, Matthieu Million, Didier Raoult, Gaetan Texier
Summary: This study evaluated the impact of doxycycline (DOXY) on body weight in military international travelers. The results showed that DOXY did not cause significant weight gain in soldiers. However, other factors such as fatty food, sedentary lifestyle, and strain-specific probiotics may contribute to weight gain.
Article
Microbiology
Malak Zoaiter, Reham Magdy Wasfy, Aurelia Caputo, Florence Fenollar, Zaher Zeaiter, Pierre-Edouard Fournier, Linda Houhamdi
Summary: Strain Marseille-Q6994 was isolated from a 72-year-old patient with pneumonia in France. Through bacterial morphology, gene sequence comparison, and genomic analysis, the study confirmed that the strain belongs to a new species, named Streptococcus bouchesdurhonensis.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Imene Ikram Hassani, Ines Quadri, Archana Yadav, Sonia Bouchard, Didier Raoult, Hocine Hacene, Christelle Desnues
Summary: This study analyzed and compared the diversity of halophilic archaea in Zehrez-Chergui (Saharian chott), Algeria, using both PCR amplification and cultivation approaches. A total of 45 distinct strains were characterized, indicating their membership in the class Halobacteria. Phylogenetic analysis revealed that 23 strains were related to 6 genera of halophilic archaea, including Halopiger which was dominant in this hypersaline environment. The molecular analysis identified a diverse range of archaeological species, highlighting the importance of combining both methods to obtain comprehensive information.
Article
Biochemistry & Molecular Biology
Malak Zoaiter, Zaher Zeaiter, Oleg Mediannikov, Cheikh Sokhna, Pierre-Edouard Fournier
Summary: Brucella intermedia, a non-fermentative bacterium, has intrinsic resistance to polymyxins, including colistin. Efflux pump inhibitors such as verapamil, reserpine, phe-arg beta-naphthylamide dihydrochloride, and carbonyl cyanide 3-chloro phenyl hydrazone were evaluated on colistin-resistant strains of B. intermedia, and it was found that the combination of CO and CCCP significantly reduced the colistin minimal inhibitory concentration, indicating the involvement of an efflux pump mechanism. Inhibitory effects on biofilm formation and a bactericidal effect were also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Rossella Maria Benvenga, Christophe Tribouilloy, Hector I. Michelena, Angelo Silverio, Florent Arregle, Helene Martel, Seyhan Denev, Yohann Bohbot, Sandrine Hubert, Sebastien Renard, Laurence Camoin, Anne Claire Casalta, Jean Paul Casalta, Frederic Gouyier, Alberto Riberi, Hubert Lepidi, Frederic Collart, Didier Raoult, Michel Drancourt, Gennaro Galasso, Daniel C. DeSimone, Rodolfo Citro, Gilbert Habb
Summary: This study aimed to investigate the clinical characteristics of different bicuspid aortic valve (BAV) morphology in infective endocarditis (IE). Patients were divided into two groups based on echocardiographic definition: right-left coronary (RL type) and right noncoronary or left noncoronary (non-RL type) cusp fusion. The study found that patients with BAV and IE may have different clinical and echocardiographic features according to valve morphology, and they tend to be referred late, even with pre-existing BAV disease.
JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY
(2023)
Article
Infectious Diseases
Estelle Sabourin, Isabelle Podglajen, Pierre-Edouard Fournier, Jean-Luc Mainardi
Summary: This study evaluated the effectiveness of the recommended treatment for endovascular infections caused by C. burnetii. The results showed that the treatment regimen with doxycycline and hydroxychloroquine achieved good clinical and biological outcomes in patients with endovascular infections.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Virology
Anna Julienne Selbe Ndiaye, Mamadou Beye, Gora Lo, Idir Kacel, Aissatou Sow, Nafissatou Leye, Abdou Padane, Aminata Mboup, Halimatou Diop-Ndiaye, Cheikh Sokhna, Coumba Toure Kane, Philippe Colson, Florence Fenollar, Souleymane Mboup, Pierre-Edouard Fournier
Summary: We used whole genome sequencing to analyze mutations in SARS-CoV-2 during the deadliest wave of the COVID-19 epidemic in Senegal. A total of 291 genotypable consensus genome sequences were obtained, revealing diversification and the presence of a Senegalese strain belonging to the P.1.14 sublineage of the Brazilian P.1 lineage. This study highlights the significant genomic variations of SARS-CoV-2 in Senegal.
Article
Infectious Diseases
Bekir Celebi, Derya Karatas Yeni, Yusuf Yilmaz, Ferhat Matur, Cahit Babur, Mehmet Ali Oktem, Mustafa Sozen, Ahmet Karatas, Didier Raoult, Oleg Mediannikov, Pierre Edouard Fournier
Summary: This study examined Borrelia species in rodents captured from rural areas of Turkey. Borrelia miyamotoi was discovered in 1.7% of the blood samples collected from wild rodents. This is the first report of B. miyamotoi presence in rodents in Turkey.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Multidisciplinary Sciences
Afaf Hamame, Reham Magdy Wasfy, Cheikh Ibrahima Lo, Florence Fenollar, Didier Raoult, Pierre-Edouard Fournier, Linda Houhamdi
Summary: Two new bacterial strains, Marseille-P2698(T) and Marseille-P2260(T), were isolated from human stools using the culturomic method. These two strains were fully described using the taxonogenomic approach. Marseille-P2698(T) was identified as a Gram-negative, motile, non-spore-forming, rod-shaped bacterium, while Marseille-P2260(T) was identified as a Gram-positive, motile, spore-forming rod-shaped bacterium.
SCIENTIFIC REPORTS
(2023)
Correction
Genetics & Heredity
Sahare Kokcha, Ajay Kumar Mishra, Jean-Christophe Lagier, Matthieu Million, Quentin Leroy, Didier Raoult, Pierre-Edouard Fournier
ENVIRONMENTAL MICROBIOME
(2023)
Letter
Infectious Diseases
Philippe Brouqui, Michel Drancourt, Didier Raoult
NEW MICROBES AND NEW INFECTIONS
(2023)
Article
Microbiology
Fatou Samba Diouf, Mamadou Beye, Mapenda Gaye, Babacar Mbaye, Stephane Alibar, Mariema Sarr, Gregory Dubourg, Jean-Christophe Lagier, Cheikh Sokhna, Florence Fenollar, Pierre-Edouard Fournier, Cheikh Ibrahima Lo
Summary: Blood is normally sterile, but bacteria in the bloodstream indicate an anomaly. By performing a blood culture on a patient with thalassemia major, a new strain of Streptococcus was isolated and identified through phenotypic, phylogenetic, and genomic characteristics analysis. Named Streptococcus thalassemiae sp. nov., this strain showed similarity to Streptococcus mitis but had significant differences based on DNA-DNA hybridization and average nucleotide identity analysis. This research demonstrates the importance of studying bacterial strains in blood cultures for accurate diagnosis.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Omar Zmerli, Sara Bellali, Gabriel Haddad, Akiko Hisada, Yusuke Ominami, Didier Raoult, Jacques Bou Khalil
Summary: Phosphotungstic acid (PTA) staining is a commonly used negative staining method in scanning electron microscopy (SEM) for assessing the viability of microorganisms. This study demonstrates that PTA staining can effectively differentiate between live and dead bacteria, providing a rapid, cost-effective, and efficient viability assay for microbiome investigations and antimicrobial susceptibility testing.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Medicine, General & Internal
Matthieu Million, Jean-Christophe Lagier, Jerome Hourdain, Frederic Franceschi, Jean-Claude Deharo, Philippe Parola, Philippe Brouqui
Summary: This study investigates the safety of using hydroxychloroquine and azithromycin as a treatment for COVID-19. The results show that with proper safety measures, no arrhythmic events occurred during the treatment period. The evaluation of electrocardiogram (ECG) and potassium levels could identify patients who should not receive this combination therapy, ensuring the safe treatment of COVID-19 patients.
MEDICINA-LITHUANIA
(2023)
Correction
Microbiology
Cheikh Ibrahima Lo, Niokhor Dione, Aminata Mbaye, Patricia Fernandez-Mellado Gomez, Issa Isaac Ngom, Camille Valles, Stephane Alibar, Jean-Christophe Lagier, Florence Fenollar, Pierre-Edouard Fournier, Didier Raoult, Seydina M. Diene
FEMS MICROBIOLOGY LETTERS
(2023)
