Article
Microbiology
Saumashish Mukherjee, Tushar Dilipchand Lodha, Jogi Madhuprakash
Summary: This study describes the xylan degradation potential and enzyme machinery of the Paenibacillus sp. LS1 strain, which is able to efficiently degrade xylan polymers from hardwood and graminaceous plants. The strain possesses an extensive repertoire of xylanolytic enzymes and is capable of utilizing xylan as the sole carbon source for growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Riteshri Soni, Sandhya Nanjani, Hareshkumar Keharia
Summary: Probiotics have emerged as an effective antibiotic substitute in animal farming, with characteristics of tolerance to gastrointestinal stress and antimicrobial properties. The genome analysis and in vitro experiments confirmed the survival and antimicrobial potential of Paenibacillus polymyxa HK4 in the gastrointestinal tract.
Article
Microbiology
Ericka Arregue de Lemos, Luciano Procopio, Fabio Faria da Mota, Diogo Jurelevicius, Alexandre Soares Rosado, Lucy Seldin
Summary: Paenibacillus antarcticus IPAC21, isolated from King George Island in Antarctica, is capable of forming endospores and producing bioemulsifiers. The genome of IPAC21 was sequenced, revealing the presence of genes related to bioemulsifier production, exopolysaccharide biosynthesis, and other metabolic pathways. The bioemulsifier produced by IPAC21 exhibited stability at different NaCl concentrations, low temperatures, and pH values, suggesting its potential application in the petroleum industry.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Bojan Papic, Margo Diricks, Darja Kusar
Summary: The study utilized whole-genome multilocus sequence typing to study the epidemiology of P. larvae, successfully identifying outbreak-related strains clusters. Whole-genome sequencing showed higher backward compatibility and discriminatory power compared to traditional methods.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Bin Chen, Hailiang Han, Junfeng Hou, Fei Bao, Heping Tan, Xiaocheng Lou, Guiyue Wang, Fucheng Zhao
Summary: In this study, an antagonist strain, named Paenibacillus polymyxa strain SF05, was isolated and found to exhibit antagonism against pathogenic fungi of maize and effectively control maize banded leaf sheath blight in the field. This strain showed high cellulase and chitinase activity, which could contribute to the degradation of fungi cell walls. The study also revealed the induction of different resistant genes in maize leaves and stems by the strain. The genome sequencing predicted genes related to the biocontrol mechanism of P. polymyxa strain SF05, including biofilm formation pathways genes, cell wall degradation enzymes, secondary metabolite biosynthesis gene clusters, and volatile organic compounds biosynthesis genes.
Article
Chemistry, Medicinal
Huiqin Huang, Zhiguo Zheng, Xiaoxiao Zou, Zixu Wang, Rong Gao, Jun Zhu, Yonghua Hu, Shixiang Bao
Summary: Carbohydrate-active enzymes (CAZymes) are an important characteristic of bacteria in marine systems. This study describes the CAZymes of a novel type species, Paenibacillus algicola HB172198(T), isolated from brown algae in Qishui Bay, Hainan, China. The genome of the strain encodes 191 CAZymes, with several putative enzymes involved in polysaccharide degradation. The alginate lyases in strain HB172198(T) show high activity at 50 degrees C and pH 8.0.
Article
Microbiology
Pedro Farias, Romeu Francisco, Lorrie Maccario, Jakob Herschend, Ana Paula Piedade, Soren Sorensen, Paula Morais
Summary: Tellurium is a metalloid that has increased interest due to its uses in emerging technologies, but mining activities and improper disposal of high-tech devices can lead to environmental issues with tellurium enrichment. Paenibacillus pabuli strain ALJ109b shows high resistance to tellurium and the ability to reduce tellurite ion efficiently, potentially serving as a tool for tellurium nanoparticle production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Riteshri Soni, Khushboo Rawal, Hareshkumar Keharia
Summary: The bacterium Paenibacillus polymyxa HK4 shows potential as a biocontrol agent and plant growth promoter, with its ability to inhibit fungal phytopathogens and enhance seed germination in cumin and groundnut. The genome analysis of HK4 reveals genetic attributes for various beneficial traits, and the presence of fusaricidin as a major antifungal metabolite is confirmed by LC-ESI-MS/MS analysis.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Aiming Jiang, Chengwu Zou, Xiang Xu, Zunwei Ke, Jiangan Hou, Guihe Jiang, Chunli Fan, Jianhua Gong, Jiguang Wei
Summary: This study reports the biocontrol efficiency of Paenibacillus peoriae HJ-2 against stem rot on Paris polyphylla and provides its complete genome sequence. HJ-2 could serve as a potential biocontrol agent against stem rot in both greenhouse and field conditions. The genome of HJ-2 contains numerous genes and gene clusters involved in plant colonization, biofilm formation, plant growth promotion, antibiotic and resistance inducer synthesis, and potential metabolic pathways that contribute to biocontrol mechanisms.
Article
Microbiology
Barbara M. Binney, Hayley Pragert, Jonathan Foxwell, Edna Gias, Meredith L. Birrell, Bernard J. Phiri, Oliver Quinn, Michael Taylor, Hye Jeong Ha, Richard J. Hall
Summary: New Zealand, a remote country in the South Pacific Ocean, introduced honey bees in 1839, which subsequently led to the discovery of American foulbrood in the 1870s. The genome sequencing of Paenibacillus larvae obtained from 164 New Zealand apiaries revealed that ST18 was the dominant sequence type, accounting for 90.2% of isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Dan Wang, Verena Poinsot, Wangxi Li, Yusheng Lu, Chong Liu, Yaying Li, Kaizhi Xie, Lili Sun, Chaohong Shi, Huanlong Peng, Wanling Li, Changmin Zhou, Wenjie Gu
Summary: This study sequenced the genome of P. mucilaginosus G78 and characterized its features. The results showed that this strain has the ability to inhibit the growth of plant pathogens, form biofilms, solubilize phosphate, and produce IAA. The analysis of genetic features suggested that it may produce exopolysaccharides consisting of glucose, mannose, galactose, and fucose. This study contributes to understanding the plant growth-promoting traits of P. mucilaginosus and its potential application in agriculture as a PGPR.
Article
Biotechnology & Applied Microbiology
Ke Ma, Wei Chen, Shi-Qing Yan, Xiao-Qi Lin, Zhen-Zhen Liu, Jia-Bao Zhang, Yu Gao, Yong-Jun Yang
Summary: This study reports a novel strain Paenibacillus jilinensis with the capability to antagonize multiple pathogens and encode abundant carbohydrate-degrading enzymes (CAZymes) and biosynthetic gene clusters (BGCs). These findings confirm the beneficial function of gut microbiota and provide a promising candidate for the development of new antibacterial agents and carbohydrate-degrading enzymes.
Article
Microbiology
Shahina Akter, Xiaoqing Wang, Sun-Young Lee, M. Mizanur Rahman, Jong-Hyun Park, Muhammad Zubair Siddiqi, Sri Renukadevi Balusamy, Kihong Nam, Md Shahedur Rahman, Md Amdadul Huq
Summary: Strain MAHUQ-46(T) is a novel bacterium capable of rapidly synthesizing ginsenoside Rd from major ginsenoside Rb1, with its genome containing carbohydrate genes related to synthesis.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Alicia C. Bertolotti, Eva Forsgren, Marc O. Schaefer, Fabrice Sircoulomb, Nicolas Gaiani, Magali Ribiere-Chabert, Laurianne Paris, Pierrick Lucas, Claire de Boisseson, Joakim Skarin, Marie-Pierre Riviere
Summary: This study introduces a standardized and robust cgMLST scheme for typing P. larvae, which allows for high-resolution differentiation and successful outbreak tracking. Compared to traditional MLST methods, cgMLST can provide finer differentiation of samples and maintains high stability and standardization for sharing results and methods across different laboratories.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Hae-In Jung, Sungkwon Park, Kai-Min Niu, Sang-Won Lee, Damini Kothari, Kwon Jung Yi, Soo-Ki Kim
Summary: SK3146, a novel strain isolated from pig feed, possesses an immense probiotic potential with a diverse range of hydrolases and bacteriocin encoding gene in its genome sequence.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2021)
Article
Virology
Philippe Colson, Philippe Gautret, Jeremy Delerce, Herve Chaudet, Pierre Pontarotti, Patrick Forterre, Raphael Tola, Marielle Bedotto, Lea Delorme, Wahiba Bader, Anthony Levasseur, Jean-Christophe Lagier, Matthieu Million, Nouara Yahi, Jacques Fantini, Bernard La Scola, Pierre-Edouard Fournier, Didier Raoult
Summary: This study investigated the dynamics of the French variant Marseille-4 of SARS-CoV-2 over a 10-month period, identifying two subvariants and analyzing their mutations and lifetimes. The findings suggest that beneficial mutations can initiate new outbreaks, while the accumulation of other mutations can result in the disappearance of lineages.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Public, Environmental & Occupational Health
Elise Klement-Frutos, Oleg Mediannikov, Pierre-Edouard Fournier, Julien Haroche, Veronique Leblond, Eric Caumes
Summary: Candidatus mycoplasma haemohominis (cMh) is a highly adapted and lethal haemoplasma infection in humans, commonly found in bats worldwide. It should be suspected in individuals with fever of unknown origin, splenomegaly, and haemolytic anaemia or haemophagocytic lymphohistiocytosis, especially among travellers in Asia Pacific. Doxycycline should be initiated as a preventive measure, with retrospective confirmation of diagnosis.
JOURNAL OF TRAVEL MEDICINE
(2023)
Review
Infectious Diseases
Myrto Koutantou, Konstantinos Kambas, Sofia Makka, Pierre-Edouard Fournier, Didier Raoult, Emmanouil Angelakis
Summary: Cat scratch disease (CSD) is difficult to diagnose, and serological assays have limitations in terms of sensitivity and specificity. Molecular assays should be considered as the gold standard for confirming CSD, and lymph node biopsy may be necessary in suspicious cases.
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2023)
Article
Microbiology
Sory Ibrahima Traore, Cheikh Ibrahima Lo, Maaloum Mossaab, Guillaume Durand, Jean Christophe Lagier, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Summary: Bacterial strain Marseille-P3954 was isolated from a stool sample of a 35-year-old male patient living in France. It belongs to a new bacterial genus, classified in a new family, based on its genomic, phenotypic, and phylogenetic evidences. The proposed classification is Maliibacterium massiliense gen. nov., sp. nov. with the type strain Marseille-P3954 (CSUR P3954 = CECT 9568).
CURRENT MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Afaf Hamame, Reham Magdy Wasfy, Cheikh Ibrahima Lo, Florence Fenollar, Didier Raoult, Pierre-Edouard Fournier, Linda Houhamdi
Summary: Two new bacterial strains, Marseille-P2698(T) and Marseille-P2260(T), were isolated from human stools using the culturomic method. These two strains were fully described using the taxonogenomic approach. Marseille-P2698(T) was identified as a Gram-negative, motile, non-spore-forming, rod-shaped bacterium, while Marseille-P2260(T) was identified as a Gram-positive, motile, spore-forming rod-shaped bacterium.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Nasserdine Papa Mze, Idir Kacel, Mamadou Beye, Raphael Tola, Mariema Sarr, Leonardo Basco, Herve Bogreau, Philippe Colson, Pierre-Edouard Fournier
Summary: A rapid and cost-effective sequencing protocol is proposed to validate its reliability by comparing the SARS-CoV-2 genome sequences obtained using the modified Illumina COVIDSeq protocol with the standard protocol. The modified protocol saves time and costs, providing high-quality results comparable to the standard protocol.
Correction
Genetics & Heredity
Sahare Kokcha, Ajay Kumar Mishra, Jean-Christophe Lagier, Matthieu Million, Quentin Leroy, Didier Raoult, Pierre-Edouard Fournier
ENVIRONMENTAL MICROBIOME
(2023)
Article
Infectious Diseases
Anna Barbiero, Tommaso Manciulli, Michele Spinicci, Iacopo Vellere, Maria Grazia Colao, Gian Maria Rossolini, Alessandro Bartoloni, Didier Raoult, Lorenzo Zammarchi
Summary: This study reports ten cases of SENLAT in Tuscany, Italy, with symptoms including scalp eschar and cervical lymphadenopathy. The difficulty of microbiological diagnosis and previous antibiotic treatment administration hindered the identification of the causative agent.
Article
Immunology
Sabrina Naud, Camille Valles, Abdourahim Abdillah, Linda Abou Chacra, Fatima Zouina Mekhalif, Ahmad Ibrahim, Aurelia Caputo, Jean-Pierre Baudoin, Frederique Gouriet, Fadi Bittar, Jean-Christophe Lagier, Stephane Ranque, Florence Fenollar, Maryam Tidjani Alou, Didier Raoult
Summary: In this study, TM7 were found to be ubiquitous members of the human oral microbiota and were also detected in other human samples, including fecal, breast milk, vaginal, and urine samples. TM7 were also detected in infectious samples, such as blood cultures and cardiac valves, at a low prevalence. The findings suggest that TM7 are human commensals and may have a role in a pathological setting, warranting further investigation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Cell Biology
Seydina M. Diene, Pierre Pontarotti, Said Azza, Nicholas Armstrong, Lucile Pinault, Eric Chabriere, Philippe Colson, Jean-Marc Rolain, Didier Raoult
Summary: Beta-lactamase enzymes are capable of conferring resistance to widely used antibiotics in human medicine, making them a topic of significant interest. Among these enzymes, the class B beta-lactamases are a type of metallo-beta-lactamase fold protein found not only in bacteria but also in other organisms. Previously unexplored in other organisms due to various barriers, modern search approaches have now uncovered their presence in different organisms including Bacteria, Archaea, Nanoarchaeota, Asgard, Humans, Giant viruses, and Candidate Phyla Radiation (CPR). These multifunctional enzymes exhibit diverse enzymatic or non-enzymatic activities, with at least thirteen reported activities such as beta-lactamase, ribonuclease, nuclease, glyoxalase, lactonase, phytase, ascorbic acid degradation, anti-cancer drug degradation, or membrane transport. This review discusses the existence of M beta L fold enzymes in different domains of life, presents suitable approaches for studying their homologous sequences in unexpected sources, and presents described M beta L fold enzymes with demonstrated enzymatic or non-enzymatic activities.
Article
Immunology
Marie Jumpertz, Jacques Sevestre, Lea Luciani, Linda Houhamdi, Pierre-Edouard Fournier, Philippe Parola
Summary: Monitoring and analyzing ticks removed from patients in Marseille, France, revealed the presence of various tick species and bacterial pathogens. Identification of the ticks and detection of bacterial DNA in the ticks can aid in clinical diagnosis, treatment, and surveillance of tickborne diseases. The most common bacterial pathogens found were Rickettsia raoultii, R. slovaca, Borrelia spp., and R. massiliae.
EMERGING INFECTIOUS DISEASES
(2023)
Article
History
Pierre Fournier
Summary: On Easter Friday in 1466, two Franciscan preachers in Cambrai preached a controversial thesis, which caused outrage. The dean of the cathedral chapter, Gilles Charlier, reacted intensely by writing and compiling a series of polemical treatises on the subject. These writings were important as they showcased a skillfully constructed polemic and provided insight into the dynamics of the Parisian university during the second half of the 15th century.
REVUE DE L HISTOIRE DES RELIGIONS
(2023)
Correction
Microbiology
Cheikh Ibrahima Lo, Niokhor Dione, Aminata Mbaye, Patricia Fernandez-Mellado Gomez, Issa Isaac Ngom, Camille Valles, Stephane Alibar, Jean-Christophe Lagier, Florence Fenollar, Pierre-Edouard Fournier, Didier Raoult, Seydina M. Diene
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Nour El Houda Mathlouthi, Hamadou Oumarou Hama, Imen Belguith, Slim Charfi, Tahya Boudawara, Jean-Christophe Lagier, Leila Ammar Keskes, Ghiles Grine, Radhouane Gdoura
Summary: In this study, the researchers performed a metagenomic analysis of colorectal cancer (CRC) patients in Tunisia to investigate the link between archaea and CRC. They found a significant association between Halobacteria, particularly Natrialba magadii, and tumor tissues in CRC patients. This study demonstrates the feasibility of archaeome analysis from formalin-fixed paraffin-embedded tissues using simple protocols and provides insight into the potential role of archaea in CRC.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
