Article
Genetics & Heredity
Yuntao Lu, Bing Liu, Junjie Ma, Shuo Yang, Ju Huang
Summary: Short-term sleep deprivation causes changes in the circadian gene expression of approximately 3,000 genes in the mouse lung, particularly in genes associated with metabolism and signaling pathways regulating protein phosphorylation. The positive correlation between mean expression and amplitude of cycling genes is maintained under sleep deprivation conditions, supporting the energetic cost optimization model of circadian gene expression.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Xudong Fang, Fuli Wen, Xiangnong Ding, Hanbang Liu, Zhiyang Chen, Zhaopeng Liu, Hongchao Liu, Wenliang Zhu, Zhongmin Liu
Summary: This study proposes a strategy for efficiently converting methane to acetic acid using CH3Cl as an intermediate. The pyridine-pretreated MOR catalyst achieves high selectivity of acetic acid and methyl acetate under moderate conditions. Water, conventionally detrimental to carbonylation reaction, was found to be conducive in this reaction system. This strategy provides a new approach for the highly efficient utilization of methane to oxygenates under mild conditions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Yaxue He, Piet N. L. Lens, Maria C. Veiga, Christian Kennes
Summary: This study investigates the use of exogenous and endogenous butyric acid to enhance butanol production during CO fermentation. Exogenous butyric acid addition resulted in the highest butanol concentration of 1.9 g/L, while CO addition triggered butanol production from endogenous butyric acid with a conversion efficiency of 58.6%. This study provides insights into strategies for increasing butanol production by CO-fed anaerobic sludge.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qinrui Liu, Hu Wang, Hualin Wang, Na Li, Ruyi He, Zhiguo Liu
Summary: This study found that disruption of the Per1/Per2 genes can decrease the reproductive capacity of elderly male mice. The mechanism involves the reduction of testosterone synthesis and sperm motility through the PKA-StAR pathway, leading to decreased fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sabrina Klemz, Thomas Wallach, Sandra Korge, Mechthild Rosing, Roman Klemz, Bert Maier, Nicholas C. Fiorenza, Irem Kaymak, Anna K. Fritzsche, Erik D. Herzog, Ralf Stanewsky, Achim Kramer
Summary: In organisms with circadian clocks, the post-translational modifications of clock proteins, particularly phosphorylation, play a crucial role in controlling circadian rhythms. Protein phosphatase 4 (PPP4) and its regulatory subunit PPP4R2 have been identified as critical components in mammals and Drosophila, affecting the circadian system by regulating phosphorylation and transactivation activity of CLOCK/BMAL1.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Xiaomeng Liu, Niuben Cao, Xinchan Liu, Yu Deng, Yu Xin, Ruobing Fu, Xirui Xin, Yubo Hou, Weixian Yu
Summary: Circadian rhythms play a crucial role in regulating the body's homeostasis, and circadian rhythm disorders can have detrimental effects on metabolic and immune functions. However, the relationship between circadian rhythm disorders and periodontitis has not been well studied. This research aimed to investigate the role of the circadian rhythm control gene BMAL1 in periodontitis. The results showed that downregulation of BMAL1 in circadian rhythm disorder-induced periodontitis led to significant alveolar bone resorption, increased osteoclast differentiation, upregulation of the inflammatory signaling molecule NF-kappa B, elevated apoptosis and oxidative stress levels in periodontal tissues. This study suggests that BMAL1 is a key regulator in periodontitis exacerbated by circadian rhythm disorders, highlighting a potential avenue for periodontitis treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Celine Jouffe, Benjamin D. Weger, Eva Martin, Florian Atger, Meltem Weger, Cedric Gobet, Divya Ramnath, Aline Charpagne, Delphine Morin-Rivron, Elizabeth E. Powell, Matthew J. Sweet, Mojgan Masoodi, N. Henriette Uhlenhaut, Frederic Gachon
Summary: Obesity and liver diseases disrupt the circadian clock regulated by BMAL1, which affects nutrient metabolism and storage. In liver fibrosis, the activity of BMAL1 is perturbed. Knockout mice lacking BMAL1 are prone to obesity but protected against insulin resistance, hepatic steatosis, inflammation, and fibrosis. The disruption of the growth hormone and sex hormone pathways plays a critical role in this protection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Yajie Guan, Manlin Xu, Zhe Zhang, Chuanming Liu, Jidong Zhou, Fei Lin, Junshun Fang, Yang Zhang, Qiuling Yue, Xin Zhen, Guijun Yan, Haixiang Sun, Wenwen Liu
Summary: Circadian disturbance, caused by factors like shift employment and nighttime light pollution, is common in modern culture. This study investigated the effects of maternal circadian disruption before pregnancy on the reproductive health of offspring in mice. It was found that the female offspring exposed to constant light showed obesity, abnormal lipid metabolism, earlier puberty onset, impaired ovarian function, and downregulated expression of various genes involved in circadian rhythm and ovarian function. This study also identified altered immune responses as a potential mechanism for the damage to ovarian function.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Harshavardhan Kenche, Meharvan Singh, Jacquez Smith, Kai Shen
Summary: Disruption of circadian rhythm leads to mitochondrial dysfunction, while the sigma-1 receptor agonist donepezil can restore mitochondrial function and rhythmicity of core clock genes, suggesting a novel treatment approach for related neurodegenerative diseases and mental disorders.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Environmental Sciences
Yaxue He, Piet N. L. Lens, Maria C. Veiga, Christian Kennes
Summary: An anaerobic mixed culture was enriched to grow on CO and syngas, producing high concentrations of butanol. Under stress conditions of CO exposure and low pH, the enrichment accumulated ethanol and high amounts of butanol, with a high butanol/butyric acid molar ratio, highlighting the efficient acid to alcohol conversion. Interestingly, butanol oxidation with simultaneous butyric acid production occurred, which has not been reported before.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Editorial Material
Cell Biology
Susmita Kaushik, Yves R. Juste, Ana Maria Cuervo
Summary: The circadian clock and chaperone-mediated autophagy (CMA) have an interdependent relationship. CMA selectively degrades core clock proteins, modulating circadian rhythm. Loss of CMA disrupts physiological circadian cycling, similar to defects observed in aging. The circadian clock temporally regulates CMA activity in different tissues, contributing to remodeling of a distinct subproteome at different times.
Article
Multidisciplinary Sciences
Shirley L. Zhang, Nicholas F. Lahens, Zhifeng Yue, Denice M. Arnold, Peter P. Pakstis, Jessica E. Schwarz, Amita Sehgal
Summary: The blood-brain barrier is critical for neural function, and this study shows that efflux of xenobiotics through the BBB follows a circadian rhythm in mice and human cells. Interestingly, limited circadian regulation of transcription was detected in brain endothelial cells, with no evident oscillations in efflux transporters. The molecular clock drives cycling of intracellular magnesium through transcriptional regulation, contributing to the rhythm in efflux.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jieyu Wu, Xu Jing, Qiqiao Du, Xiaoting Sun, Kristian Holgersson, Juan Gao, Xingkang He, Kayoko Hosaka, Chen Zhao, Wei Tao, Garret A. FitzGerald, Yunlong Yang, Lasse D. Jensen, Yihai Cao
Summary: This study demonstrates that disruption of the circadian rhythm by genetic deletion of the Bmal1 gene in mice enhances fibrosis in tumors, leading to increased tumor growth and metastatic potential. The deletion of Bmal1 results in decreased expression of its target gene PAI-1, which leads to activation of plasmin and subsequent fibrosis and transition of cancer-associated fibroblasts. Inhibiting the TGF-beta signaling pathway effectively reduces the metastatic potential of several types of cancer. These findings provide novel insights into the role of the circadian clock in tumor growth and metastasis, suggesting that normalization of the circadian rhythm could be a potential therapeutic strategy for cancer.
Article
Biology
Suil Kim, Douglas G. McMahon
Summary: This study reveals that light input to the SCN can change the waveform of molecular clockworks, marking clock advances and delays at simulated dawn and dusk. The changes in waveform seem to be greatest in the ventrolateral SCN, while the effects on period are greatest in the dorsomedial SCN, suggesting spatiotemporally distinct responses within the SCN neural network.
Article
Gastroenterology & Hepatology
Min Chen, Yanke Lin, Yongkang Dang, Yifei Xiao, Fugui Zhang, Guanghui Sun, Xuejun Jiang, Li Zhang, Jianhao Du, Shuyi Duan, Xiaojian Zhang, Zifei Qin, Jing Yang, Kaisheng Liu, Baojian Wu
Summary: This study reveals that the intestinal clock plays a crucial role in regulating liver rhythmicity and metabolism. The loss of the intestinal clock leads to a large-scale reprogramming of the liver transcriptome and alters hepatic metabolism. The intestinal clock controls the rhythmicity of the hepatic transcription factor SREBP-1c, which further affects hepatic lipogenesis and gluconeogenesis. These findings suggest that targeting intestinal rhythms may be a new approach for improving metabolic health.
JOURNAL OF HEPATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hiroaki Kitagishi, Kazuki Ohara, Daiki Shimoji, Maxime Vonesch, Jean Weiss, Jennifer A. Wytko
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
(2019)
Article
Chemistry, Medicinal
Anthony Ollivier, Roberta Foresti, Zeina El Ali, Thierry Martens, Hiroaki Kitagishi, Roberto Motterlini, Michael Rivard
Article
Chemistry, Multidisciplinary
Hiroaki Kitagishi, Mai Saito, Qiyue Mao, Akiko Kiriyama, Shigeru Negi, Koji Kano
CHEMISTRY-AN ASIAN JOURNAL
(2019)
Article
Biology
Qiyue Mao, Akira T. Kawaguchi, Shun Mizobata, Roberto Motterlini, Roberta Foresti, Hiroaki Kitagishi
Summary: Mao et al. reported a highly sensitive quantification of carbon monoxide using a simple colorimetric assay with the synthetic supramolecular compound hemoCD1. This method can detect CO distribution in organs, including the brain, and can also function as a CO scavenger to eliminate residual CO accumulated in organs. The authors also demonstrated the protective role of circulating hemoglobin in CO intoxication.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Qiyue Mao, Pradip K. Das, Stephane Le Gac, Bernard Boitrel, Vincent Dorcet, Koji Oohora, Takashi Hayashi, Hiroaki Kitagishi
Summary: Fe(III)Por-1 was synthesized to mimic the function of myoglobin in aqueous solution, forming a stable inclusion complex with Py3OCD. Fe(II)PorCD-1 and Fe(II)PorCD-2 showed differences in O2 and CO binding, with the strapped structure hindering the entrance of gaseous ligands into the iron(II) center.
INORGANIC CHEMISTRY
(2021)
Review
Polymer Science
Hiroaki Kitagishi, Qiyue Mao
Summary: hemoCD1, a supramolecular complex constructed as a synthetic Hb/Mb model in aqueous solution, shows high affinity to bind oxygen and carbon monoxide, making it valuable for selectively depleting CO in vivo and quantifying CO accumulation in organs, as well as serving as an antidote for CO poisoning in animals.
Article
Chemistry, Multidisciplinary
Rino Tachikawa, Hiroki Saito, Hajime Moteki, Mitsutoshi Kimura, Hiroaki Kitagishi, Florencio Arce Jr, Gerard Lee See, Takashi Tanikawa, Yutaka Inoue
Summary: The study prepared inclusion complexes of S-allylcysteine and cyclodextrin by freeze-drying method and investigated their effect on liver tumor cells. Results showed different stability and cell proliferation inhibition among different types of SAC and cyclodextrin complexes.
Article
Chemistry, Multidisciplinary
Qiyue Mao, Hiroaki Kitagishi
Summary: This study describes the unexpected host-guest inclusion complex formation between tetra-PEGylated tetraphenylporphyrin and a per-O-methylated cyclodextrin (CD) dimer through molecular threading. Despite the size difference between the PEGylated porphyrin and the CD dimer, a 1:1 inclusion complex was spontaneously formed in water. The inclusion complex showed long circulation in blood and displayed unique host-guest exchange reaction from a 1/2 inclusion complex to a 1/1 complex.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Multidisciplinary Sciences
Qiyue Mao, Xuansu Zhao, Akiko Kiriyama, Shigeru Negi, Yasutaka Fukuda, Hideki Yoshioka, Akira T. Kawaguchi, Roberto Motterlini, Roberta Foresti, Hiroaki Kitagishi
Summary: In this study, an injectable antidote against simultaneous poisoning by carbon monoxide (CO) and hydrogen cyanide (CN-) was invented. The antidote showed remarkable protective effects in mouse and rat models, improving survival rates and reducing CO and CN- levels in the blood. Pharmacokinetic data demonstrated fast urinary excretion and short elimination half-life of the antidote. Furthermore, the antidote was effective in improving survival and promoting rapid recovery from physical incapacitation in a simulated fire accident scenario.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Masataka Noguchi, Qiyue Mao, Atsuki Nakagami, Hiroaki Kitagishi
Summary: Iron(ii/iii) porphyrin/cyclodextrin inclusion complexes are used as hemoprotein models in vivo. Our study found that the iron(iii) porphyrin complex is spontaneously reduced to the iron(ii) state in mouse circulation. The reduced complex binds endogenous CO from carboxyhemoglobin and is excreted in urine. The natural reduction system is effective for synthetic heme-model compounds.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Mian Tang, Yao-Hua Liu, Hua Liu, Qiyue Mao, Qilin Yu, Hiroaki Kitagishi, Ying-Ming Zhang, Lehui Xiao, Yu Liu
Summary: The advent of macrocycle-based supramolecular chemistry offers powerful strategies for regulating bioactivities in living systems. A suprabiomacromolecular nanosystem involving microtubules, cell-permeable porphyrins, and antimitotic peptide-decorated permethyl-beta-cyclodextrins is constructed to promote cell apoptosis. This innovative approach combines complexation-promoted photodynamic efficacy with controlled aggregation of natural biomacromolecules.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Hiroaki Kitagishi, Koji Kano
Summary: Myoglobin (Mb) is an optimal system for capturing molecular oxygen in aqueous solution, but stable O-2 adducts are challenging to obtain due to immediate autoxidation by water molecules. HemoCD1, a supramolecule, satisfies the challenging requirements for an artificial O-2 receptor and is capable of function in aqueous solution. This research has expanded to include synthesizing analogues, modeling enzymatic reactions, developing artificial oxygen carriers, and selective binding/removal of toxic small molecules in living organisms.
CHEMICAL COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Hiroaki Kitagishi, Misa Jiromaru, Naomi Hasegawa
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Ryuhei Nishiyabu, Yuki Takahashi, Taro Yabuki, Shoji Gommori, Yuki Yamamoto, Hiroaki Kitagishi, Yuji Kubo
Article
Chemistry, Multidisciplinary
Qiyue Mao, Hiroaki Kitagishi
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
(2019)