Article
Virology
Michelle E. Wong, Chad J. Johnson, Anna C. Hearps, Anthony Jaworowski
Summary: In this study, a robust experimental model was established to quantify and investigate HIV reactivation in latently infected macrophages, showing that different macrophage phenotypes, cellular and tissue environments influence HIV reactivation. Additionally, the study demonstrated that certain latency-reversing agents may have different effects on latently infected macrophages compared to T cells, suggesting the need for dedicated strategies to target these populations in vivo.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Prapakorn Wisitpongpun, Pachuen Potup, Kanchana Usuwanthim
Summary: This study investigated the effects of oleamide on macrophage polarization and NLRP3 inflammasome activation. The results showed that oleamide promoted M1 macrophage polarization and increased IL-1 beta production. Additionally, oleamide appeared to activate the NLRP3 inflammasome as a second signal. These findings reveal a new function for oleamide and potential targets for treating NLRP3-related inflammatory disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Paul Chaintreuil, Emeline Kerreneur, Maxence Bourgoin, Coline Savy, Cecile Favreau, Guillaume Robert, Arnaud Jacquel, Patrick Auberger
Summary: Macrophages can adopt various phenotypes and play important roles in immune response, inflammation, and tissue repair. In human tissues, there are three main subpopulations of macrophages: naive macrophages, pro-inflammatory macrophages, and anti-inflammatory macrophages. Naive macrophages display phagocytic functions and can polarize into pro or anti-inflammatory macrophages. Pro-inflammatory macrophages are involved in the inflammatory response, while anti-inflammatory macrophages aid in the resolution of inflammation and tissue repair. Understanding the molecular mechanisms of macrophage generation, activation, and polarization is crucial for developing new therapeutic strategies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jake W. Rhodes, Rachel A. Botting, Kirstie M. Bertram, Erica E. Vine, Hafsa Rana, Heeva Baharlou, Peter Vegh, Thomas R. O'Neil, Anneliese S. Ashhurst, James Fletcher, Grant P. Parnell, J. Dinny Graham, Najla Nasr, Jake J. K. Lim, Laith Barnouti, Peter Haertsch, Martijn P. Gosselink, Angelina Di Re, Faizur Reza, Grahame Ctercteko, Gregory J. Jenkins, Andrew J. Brooks, Ellis Patrick, Scott N. Byrne, Eric Hunter, Muzlifah A. Haniffa, Anthony L. Cunningham, Andrew N. Harman
Summary: The study examines the interaction of HIV with sub-epithelial MNPs in anogenital tissues and identifies two subsets, CD14(+)CD1c(+) monocyte-derived dendritic cells and langerin-expressing conventional dendritic cells 2, that preferentially take up and transmit the virus to CD4 T cells.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Benxin Gong, Ying Zheng, Jiahua Li, Huafeng Lei, Kexin Liu, Jingyun Tang, Yanrong Peng
Summary: This study found that luteolin promotes M2 macrophage polarization and inhibits M1 macrophage polarization, while glycyrrhizic acid does not have these effects. Furthermore, it was discovered that luteolin achieves this regulation by upregulating the expression of hsa_circ_0001326.
Article
Cell Biology
Melissa Parny, Jose Bernad, Melissa Prat, Marie Salon, Agnes Aubouy, Elsa Bonnafe, Agnes Coste, Bernard Pipy, Michel Treilhou
Summary: Ziram affects macrophage functions and polarization, increasing phagocytosis and inducing oxidative stress which leads to the expression of antioxidant genes. Ziram modulates macrophages to M2-like anti-inflammatory phenotype through zinc, potentially associated with various diseases.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Microbiology
Jennillee Wallace, Hemil Gonzalez, Reshma Rajan, Srinivas D. Narasipura, Amber K. Virdi, Arnold Z. Olali, Ankur Naqib, Zarema Arbieva, Mark Maienschein-Cline, Lena Al-Harthi
Summary: Combination antiretroviral therapy (cART) has significantly improved the treatment of HIV/AIDS, but it may also affect cell dysregulation and increase susceptibility to comorbidities in persons living with HIV (PLWH).
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Oncology
Paul Chaintreuil, Lucie Laplane, Florian Esnault, Victoria Ghesquier, Coline Savy, Nathan Furstoss, Marie-Laure Arcangeli, Thomas Cluzeau, Guillaume Robert, Nathalie Droin, Eric Solary, Patrick Auberger, Arnaud Jacquel
Summary: Macrophages are innate immune cells that play crucial roles in various physiological and pathological processes. Caspases, previously known for their involvement in programmed cell death, are found to have non-apoptotic functions in macrophage differentiation and inflammation. Emricasan, a pan-caspase inhibitor, has shown potential in reprogramming monocyte-derived macrophages and could be an alternative therapy for diseases driven by these macrophages.
Article
Virology
Laurent Hany, Marc-Olivier Turmel, Corinne Barat, Michel Ouellet, Michel J. Tremblay
Summary: Although LRAs can efficiently reactivate latent CD4(+) T cells, their impact on HIV-1 infection and production in macrophages remains unclear. This study found that certain LRAs exhibit antireplicative features in human macrophages and identified a new viral production inhibition mechanism by bryostatin-1 specific to myeloid cells. Further research is needed to fully assess the efficacy of HIV-1 eradicating strategies.
JOURNAL OF VIROLOGY
(2022)
Article
Medicine, General & Internal
Mohamed Bassiouni, Philipp Arens, Samira Ira Zabaneh, Heidi Olze, David Horst, Florian Rossner
Summary: This study found that the polarization phenotype of macrophages in cholesteatoma is associated with the severity of the disease, particularly the number of M1 cells and the M1/M2 ratio are significantly correlated with the extent of ossicular erosion.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yen-Chieh Chuang, Shu-Yu Wu, Yu-Chuan Huang, Chung-Kan Peng, Shih-En Tang, Kun-Lun Huang
Summary: In this study, the role of aquaporins (AQPs) in macrophage polarization was investigated. The AQP inhibitor mercury chloride (HgCl2) was found to restrict cell volume change, suppress the p38 MAPK/NF kappa B pathway, promote autophagy, and influence macrophage M1 polarization.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Infectious Diseases
Serena Di Vincenzo, Maria Ferraro, Simona Taverna, Velia Malizia, Marco Buscetta, Chiara Cipollina, Valentina Lazzara, Paola Pinto, Marco Bassano, Stefania La Grutta, Elisabetta Pace
Summary: Macrophage polarization is a process where specific features are acquired by macrophages, represented by M1 and M2 polarization. M1 macrophages include IL-6, IL-1 beta, IL-12, and IL-8, while M2 cytokines include TGF-beta. This study explores the effects of tyndallized bacteria (TB) on macrophage polarization.
Article
Medicine, Research & Experimental
P. Vishnyakova, A. Poltavets, E. Karpulevich, A. Maznina, V. Vtorushina, L. Mikhaleva, E. Kananykhina, A. Lokhonina, S. Kovalchuk, A. Makarov, A. Elchaninov, G. Sukhikh, T. Fatkhudinov
Summary: Macrophages play a crucial role in innate immunity and are activated by various factors. This study found that macrophages derived from CD14+ monocytes exhibit higher sensitivity to pro-inflammatory induction, showing increased levels of pro-inflammatory and anti-inflammatory cytokines compared to CD16+-derived macrophages. This data may be valuable for macrophage-based cell therapy applications, providing insights for manipulating donor monocytes for transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Immunology
Yingqian Mi, Mengyan Tang, Qiong Wu, Yinan Wang, Qihui Liu, Pei Zhu, Xiaoyang Xue, Yuntong Liu, Xinyu Chai, Yuyang Hou, Dongmei Yan
Summary: BCG therapy can induce macrophage polarization to the M1 type, and NMAAP1 plays a crucial role in this process by regulating glycolysis and HIF-1α expression. This promotes the antitumor effect of macrophages.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Immunology
Carmen W. E. Embregts, Annelieke S. Wentzel, Alexander T. den Dekker, Wilfred F. J. van IJcken, Ralph Stadhouders, Corine H. GeurtsvanKessel
Summary: Macrophages play a crucial role in the immune response to rabies virus (RABV). This study revealed that RABV induces activation and polarization of macrophages, leading to an antiviral response. The RABV-stimulated macrophages express multiple signature genes of M1, M2a, and M2c phenotypes, and activate pathways related to interferon and antiviral response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Sport Sciences
Matteo Bonato, Filippo Turrini, Valentina De Zan, Andrea Meloni, Marta Plebani, Elena Brambilla, Alessandra Giordani, Chiara Vitobello, Roberta Caccia, Maria Francesca Piacentini, Antonio La Torre, Adriano Lazzarin, Giampiero Merati, Laura Galli, Paola Cinque
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
(2020)
Article
Biochemistry & Molecular Biology
Marco Pio La Manna, Valentina Orlando, Teresa Prezzemolo, Paola Di Carlo, Antonio Cascio, Giovanni Delogu, Guido Poli, Lucy C. Sullivan, Andrew G. Brooks, Francesco Dieli, Nadia Caccamo
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2020)
Review
Environmental Sciences
Matteo Bonato, Filippo Turrini, Laura Galli, Giuseppe Banfi, Paola Cinque
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Biochemical Research Methods
Greta Forlani, Justine Michaux, HuiSong Pak, Florian Huber, Elodie Lauret Marie Joseph, Elise Ramia, Brian J. Stevenson, Michael Linnebacher, Roberto S. Accolla, Michal Bassani-Sternberg
Summary: The study revealed the diversity of HLA ligandome in GBM cell lines and the significant impact of CIITA expression on HLA-II presentation machinery compared to IFN gamma treatment. It contributes to the identification of novel HLA-I and HLA-II ligands derived from known GBM-associated tumor antigens and suggests that CIITA-expressing GBM cells acquire an antigen presenting cell-like phenotype.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Infectious Diseases
Francesca Ferretti, Valentina De Zan, Simonetta Gerevini, Filippo Turrini, Enzo Boeri, Nicola Gianotti, Hamid Hasson, Adriano Lazzarin, Paola Cinque
CURRENT HIV/AIDS REPORTS
(2020)
Letter
Dentistry, Oral Surgery & Medicine
Lorenzo Azzi, Marco Toia, Nicole Stevanello, Fabrizio Maggi, Greta Forlani
Review
Biochemistry & Molecular Biology
Greta Forlani, Mariam Shallak, Roberto Sergio Accolla, Maria Grazia Romanelli
Summary: Cellular and animal models play important roles in studying HTLV-1-associated diseases; cell models can be used to study the mechanisms of HTLV proteins, while animal models can be used to recapitulate pathological processes and validate the effectiveness of treatment methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Isabel Pagani, Guido Poli, Elisa Vicenzi
Summary: Upon viral invasion, host cells activate defense mechanisms to prevent further viral propagation through the production of type I interferons and activation of IFN-stimulated genes. TRIM22, as a member of the TRIM family, plays a crucial role in antiviral defense by targeting various DNA and RNA viruses, such as HIV-1 and IAV, through direct or indirect interactions with viral components at both protein and chromatin levels.
Article
Virology
Filippo Fronza, Nelli Groff, Angela Martinelli, Beatrice Zita Passerini, Nicolo Rensi, Irene Cortelletti, Nicolo Vivori, Valentina Adami, Anna Helander, Simone Bridi, Michael Pancher, Valentina Greco, Sonia Iolanda Garritano, Elena Piffer, Lara Stefani, Veronica De Sanctis, Roberto Bertorelli, Serena Pancheri, Lucia Collini, Erik Dassi, Alessandro Quattrone, Maria Rosaria Capobianchi, Giancarlo Icardi, Guido Poli, Patrizio Caciagli, Antonio Ferro, Massimo Pizzato
Summary: Efficient and wide-scale testing for SARS-CoV-2 is crucial for monitoring infection rates in the community. This study used a novel saliva collection device and found that saliva testing performed well in symptomatic individuals but showed a discordance in samples from convalescent subjects. It was equally effective as nasopharyngeal swabs for contact tracing in asymptomatic individuals.
Article
Biochemistry & Molecular Biology
Isabel Pagani, Pietro Demela, Silvia Ghezzi, Elisa Vicenzi, Massimo Pizzato, Guido Poli
Summary: In addition to CD4(+) T lymphocytes, myeloid cells and differentiated macrophages are targets of HIV-1 infection, and there are significant differences in virus replication between the two cell types. Understanding these differences is crucial for evaluating the role of cell-associated restriction factors in preventing virus replication in macrophages and T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Pilotti, Attilio Cannata, Giacomo Magnani, Fabio Bignami, Andrea Corsi, Maria Teresa Valenti, Mariam Shallak, Greta Forlani, Maria Grazia Romanelli
Summary: This study identified a signature of eight miRNAs associated with HTLV-2 infection and found that altered miRNA expression patterns were correlated with impaired Th cell differentiation and cytokine signaling pathways. Additionally, the study showed that the RUNX2 protein was significantly more expressed in the presence of HTLV-2 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Isabel Pagani, Linda Ottoboni, Paola Podini, Silvia Ghezzi, Elena Brambilla, Svetlana Bezukladova, Davide Corti, Marco Emilio Bianchi, Maria Rosaria Capobianchi, Guido Poli, Paola Panina-Bordignon, Edwin A. Yates, Gianvito Martino, Elisa Vicenzi
Summary: Heparin exhibits neuroprotective effects against Zika virus infection by inhibiting cell death, necrosis and apoptosis, and preserving cell differentiation, while also inhibiting virus replication.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valentina Granata, Isabel Pagani, Emanuela Morenghi, Maria Lucia Schiavone, Alessandra Lezzi, Silvia Ghezzi, Elisa Vicenzi, Guido Poli, Cristina Sobacchi
Summary: Preliminary evidence suggests an interaction between NBAS and NBAS-related functions and SARS-CoV-2 in infected cells, as observed in the early time points after infection of human lung epithelial cell line Calu3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Isabel Pagani, Linda Ottoboni, Paola Panina-Bordignon, Gianvito Martino, Guido Poli, Sarah Taylor, Jeremy E. Turnbull, Edwin Yates, Elisa Vicenzi
Summary: This study investigates the effects of chemically modified heparin derivatives with reduced anticoagulant activities on ZIKV infection, and finds that they can prevent cell death and inhibit ZIKV replication in infected neural progenitor cells. The combination of heparin with Sofosbuvir shows a synergistic effect.
Article
Immunology
Christina K. Psomas, Karl Salzwedel, Mario Stevenson, Guido Poli, Jean-Pierre Routy, David Margolis, Nicolas Chomont, Alain Lafeuillade
JOURNAL OF VIRUS ERADICATION
(2020)