Article
Immunology
Deborah M. Stiffler, Janet Oyieko, Carolyne M. Kifude, David M. Rockabrand, Shirley Luckhart, V. Ann Stewart
Summary: This study in Kombewa, Kenya found that individuals infected with HIV-1 were more likely to carry gametocytes, with significantly higher gametocyte copy numbers. In addition, HIV-1 positivity was associated with higher gametocyte prevalence in men and increased gametocyte carriage with age.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sourav Ghosh, Rajib Kundu, Manjunatha Chandana, Rahul Das, Aditya Anand, Subhashree Beura, Ruchir Chandrakant Bobde, Vishal Jain, Sowmya Ramakant Prabhu, Prativa Kumari Behera, Akshaya Kumar Mohanty, Mahabala Chakrapani, Kapaettu Satyamoorthy, Amol Ratnakar Suryawanshi, Anshuman Dixit, Govindarajan Padmanaban, Viswanathan Arun Nagaraj
Summary: The malaria parasite lacks a traditional pathway for synthesizing amino acids and relies on hemoglobin degradation and external resources for amino acids. Surprisingly, it retains a putative gene for glutamine synthetase (GS), despite glutamine being abundant in the human and mosquito hosts. This study reveals that Plasmodium GS has evolved as a unique type I enzyme with specific structural and regulatory properties suited for the asexual niche.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Janette Reader, Mariette E. van der Watt, Lyn-Marie Birkholtz
Summary: This study optimized a simple and cost-effective strategy for describing the stage-specific action of antimalarial compounds against different developmental stages of the malaria parasite.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Lisette Meerstein-Kessel, Jeron Venhuizen, Daniel Garza, Nicholas I. Proellochs, Emma J. Vos, Joshua M. Obiero, Philip L. Felgner, Robert W. Sauerwein, Marynthe Peters, Annie S. P. Yang, Martijn A. Huynen
Summary: Plasmodium species, the causative agent of malaria, have a complex life cycle involving two hosts. The sporozoite life stage is characterized by specific proteins such as type II fatty acid synthesis and GPI anchor synthesis. Sporozoites in the salivary glands of mosquitos appear metabolically relatively inactive, and sporozoite-specific proteins elicit antibodies in the human host.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Immunology
Lena Roling, Ansgar Flammersfeld, Gabriele Pradel, Sandra Bennink
Summary: The WD40-repeat protein PfWLP1 plays a crucial role in interacting with adhesion protein complexes in the malaria parasite, essential for the stability and maturation of gametocytes, affecting exflagellation ability.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lela Jackson, Madison Rennie, Alison Poussaint, Suzanne Scarlata
Summary: The G alpha q/PLC beta 1 signaling system plays a crucial role in the formation and transcription of stress granules, with different types of stress leading to unique protein compositions in the stress granules.
SCIENTIFIC REPORTS
(2022)
Review
Biotechnology & Applied Microbiology
Cathrin Grawe, Suzan Stelloo, Femke A. H. van Hout, Michiel Vermeulen
Summary: The study of RNA-protein interactions has revealed many unknown RNA-binding proteins, but the specific protein-RNA transcript interactions remain unclear. Challenges include identifying which proteins bind to specific RNA transcripts, and future directions may involve using CRISPR-RNA targeting systems to study endogenous RNA-protein interactions.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lakshmi Prabha Venugopalan, Vishukumar Aimanianda, Venkatesh Prajna Namperumalsamy, Lalitha Prajna, Dharmalingam Kuppamuthu, Jeya Maheshwari Jayapal
Summary: In this study, the importance of Aspergillus flavus and Aspergillus fumigatus as human pathogens was highlighted. The surface-associated proteins and early exoproteome of these two pathogens were analyzed and compared, revealing potential species-specific signature proteins. Transcript analysis showed that the correlation between transcript and protein levels varied among different proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Pengge Qian, Xu Wang, Cuirong Guan, Xin Fang, Mengya Cai, Chuan-qi Zhong, Yong Cui, Yanbin Li, Luming Yao, Huiting Cui, Kai Jiang, Jing Yuan
Summary: This study reveals the structure and function of the apical polar ring (APR) in the Plasmodium ookinete, as well as the mechanism by which APR mediates the apical positioning of subpellicular microtubules (SPMTs). This has important implications for understanding the morphogenesis and transmission of malaria parasites.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Sumit Mukherjee, Suong Nguyen, Eashan Sharma, Daniel E. Goldberg
Summary: Egress and invasion of the malaria parasite Plasmodium are controlled by effector proteins regulated by the aspartic protease plasmepsin X (PM X). This study maps the self-cleavage sites of PM X and shows that the N-terminal part of its prodomain is crucial for proper intracellular trafficking.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Seth P. Miller, George Maio, Xiaoyu Zhang, Felix S. Badillo Soto, Julia Zhu, Stephen Z. Ramirez, Hening Lin
Summary: Active mutations in the RAS genes are common in human cancers. This study used proteomics approach to identify isoform-specific interactors of RAS and discovered potential effector proteins. These interacting proteins are important for cancer cells, and inhibiting them can effectively inhibit cancer growth.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Reka Babai, Richard Izrael, Beata G. Vertessy
Summary: Understanding the molecular background of genomic integrity maintenance is crucial for understanding the rapid adaptation of the malaria parasite, Plasmodium falciparum, to antimalarials. This study focused on the nucleotide pools in different stages of the parasite and found significant differences in the ratio of nucleotides between stages. The findings also revealed the impact of a drug on nucleotide levels that were previously unknown.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Claire L. Wishart, Alanna G. Spiteri, Giuseppe Locatelli, Nicholas J. C. King
Summary: Microglia and bone marrow-derived monocytes play important roles in CNS inflammation, but the focus on specific cell types or disease models has limited our understanding of common and disease-specific responses. This meta-analysis integrates transcriptomic data from disease models to provide a comprehensive view of myeloid responses across CNS disease. It reveals that the microglial and monocyte program is influenced by the disease environment, and different disease settings lead to expansion of unique myeloid subpopulations. These subpopulations have specific functional states and are conserved across CNS pathology. The resource also identifies a new neuroinflammatory-stable gene, Cd81, that accurately distinguishes microglia from monocyte-derived cells.
Article
Microbiology
Jan Stephan Wichers, Paolo Mesen-Ramirez, Gwendolin Fuchs, Jing Yu-Strzelczyk, Jan Staecker, Heidrun von Thien, Arne Alder, Isabelle Henshall, Benjamin Liffner, Georg Nagel, Christian Loew, Danny Wilson, Tobias Spielmann, Shiqiang Gao, Tim-Wolf Gilberger, Anna Bachmann, Jan Strauss
Summary: This study investigated the subcellular localization and essentiality of several membrane transport proteins in Plasmodium falciparum parasites. Four of the membrane transport proteins were found to be essential for asexual development, and the plasma membrane resident transporter 1 was shown to be essential for gametocytogenesis.
Article
Multidisciplinary Sciences
Thomas Hollin, Steven Abel, Alejandra Falla, Charisse Flerida A. Pasaje, Anil Bhatia, Manhoi Hur, Jay S. Kirkwood, Anita Saraf, Jacques Prudhomme, Amancio De Souza, Laurence Florens, Jacquin C. Niles, Karine G. Le Roch
Summary: Using high-throughput functional genomics, including metabolomics, Hollin et al. identified two essential RAP proteins in Plasmodium falciparum and revealed their role in controlling mitochondrial rRNAs.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Su Wang, Jan M. Friedman, Per Suppa, Ralph Buchert, Victor-Felix Mautner
Summary: The study found that total brain volume and white matter volume were increased in adults with NF1, while grey matter volume decreased faster with age compared to controls. Greater brain volumes were associated with lower attention deficits and higher intelligence quotients in adults with NF1.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Cell Biology
Kathryn Westendorf, Stefanie Zentelis, Lingshu Wang, Denisa Foster, Peter Vaillancourt, Matthew Wiggin, Erica Lovett, Robin van der Lee, Joerg Hendle, Anna Pustilnik, J. Michael Sauder, Lucas Kraft, Yuri Hwang, Robert W. Siegel, Jinbiao Chen, Beverly A. Heinz, Richard E. Higgs, Nicole L. Kallewaard, Kevin Jepson, Rodrigo Goya, Maia A. Smith, David W. Collins, Davide Pellacani, Ping Xiang, Valentine de Puyraimond, Marketa Ricicova, Lindsay Devorkin, Caitlin Pritchard, Aoise O'Neill, Kush Dalal, Pankaj Panwar, Harveer Dhupar, Fabian A. Garces, Courtney A. Cohen, John M. Dye, Kathleen E. Huie, Catherine V. Badger, Darwyn Kobasa, Jonathan Audet, Joshua J. Freitas, Saleema Hassanali, Ina Hughes, Luis Munoz, Holly C. Palma, Bharathi Ramamurthy, Robert W. Cross, Thomas W. Geisbert, Vineet Menachery, Kumari Lokugamage, Viktoriya Borisevich, Iliana Lanz, Lisa Anderson, Payal Sipahimalani, Kizzmekia S. Corbett, Eun Sung Yang, Yi Zhang, Wei Shi, Tongqing Zhou, Misook Choe, John Misasi, Peter D. Kwong, Nancy J. Sullivan, Barney S. Graham, Tara L. Fernandez, Carl L. Hansen, Ester Falconer, John R. Mascola, Bryan E. Jones, Bryan C. Barnhart
Summary: A monoclonal antibody named LY-CoV1404 has been successfully isolated, which demonstrates highly potent neutralization against multiple variants of the SARS-CoV-2 virus. The binding site of LY-CoV1404 remains relatively conserved, allowing it to maintain its activity. This finding suggests that LY-CoV1404 has the potential to be an effective therapeutic agent against all known variants.
Review
Biochemistry & Molecular Biology
G. A. C. Franken, M. A. Huynen, L. A. Martinez-Cruz, R. J. M. Bindels, J. H. F. de Baaij
Summary: Magnesium plays a vital role in cellular processes and disturbances in its levels can result in cell growth delay and metabolic defects. This review compares the structure and function of magnesium transporters in prokaryotes and eukaryotes, highlighting the conserved motifs and selectivity pore. It also discusses the unique features of TRP channels in eukaryotes that contribute to magnesium transport capacity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Michele Brischigliaro, Alfredo Cabrera-Orefice, Mattia Sturlese, Dei M. Elurbe, Elena Frigo, Erika Fernandez-Vizarra, Stefano Moro, Martijn A. Huynen, Susanne Arnold, Carlo Viscomi, Massimo Zeviani
Summary: The composition of cytochrome c oxidase (COX) is functionally conserved between vertebrate and invertebrate species, despite potential differences in individual structures.
Article
Immunology
Roos M. de Jong, Manon Alkema, Tate Oulton, Elin Dumont, Karina Teelen, Rie Nakajima, Rafael Ramiro de Assis, Kathleen W. Dantzler Press, Priscilla Ngotho, Kevin K. A. Tetteh, Phil Felgner, Matthias Marti, Katharine A. Collins, Chris Drakeley, Teun Bousema, Will J. R. Stone
Summary: This study investigates the antibody responses to sexual stage antigens in individuals infected with P. falciparum. The results show that even after exposure to relatively low gametocyte densities, a significant anti-sexual stage humoral response is induced in malaria-naive individuals. The study identifies novel antigens associated with gametocyte exposure, which can be used for surveillance of the malaria infectious reservoir and support vaccine development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Roos M. de Jong, Susheel K. Singh, Karina Teelen, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Will J. R. Stone, Emily Locke, Jordan Plieskatt, Michael Theisen, Teun Bousema, Matthijs M. Jore
Summary: Researchers analyzed sera from naturally exposed individuals and found that the presence and/or intensity of antibodies against 12 novel putative surface expressed gametocyte antigens was associated with transmission reducing activity. Protein fragments of these antigens were successfully designed and expressed in different cells. All tested antigens were recognized by antibodies from individuals living in malaria-endemic areas and induced antigen-specific antibody responses in mice. However, none of these antigens induced an antibody response that reduced parasite development in the mosquito midgut.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Microbiology
Mariano A. Molina, Britt A. Coenen, William P. J. Leenders, Karolina M. Andralojc, Martijn A. Huynen, Willem J. G. Melchers
Summary: Cervical cancer is the third leading cause of female cancers globally, primarily caused by persistent infections with high-risk human papillomaviruses. The composition of the cervicovaginal microenvironment plays a role in hrHPV infections, disease progression, and regression.
Article
Public, Environmental & Occupational Health
Wouter Graumans, Michelle Schinkel, Geert-Jan van Gemert, Jeroen Spitzen, Teun Bousema, Pascal Miesen
Summary: This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system for malaria research. The results showed that there was a loss in transmission when using the Hemotek feeder with a conventional collagen membrane, but there was no difference in transmission between both feeders when Parafilm was used as the membrane. Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Immunology
Surafel K. Tebeje, Wakweya Chali, Elifaged Hailemeskel, Jordache Ramjith, Abrham Gashaw, Temesgen Ashine, Desalegn Nebret, Endashaw Esayas, Tadele Emiru, Tizita Tsegaye, Karina Teelen, Kjerstin Lanke, Eizo Takashima, Takafumi Tsuboi, Nichole D. Salinas, Niraj H. Tolia, David Narum, Chris Drakeley, Benoit Witkowski, Amelie Vantaux, Matthijs M. Jore, William J. R. Stone, Ivo S. Hansen, Fitsum G. Tadesse, Teun Bousema
Summary: This study investigated the association between antibody prevalence and the infectivity of Plasmodium vivax to mosquitoes. The results showed that antibodies against Pvs47, Pvs230, and Pvs25 were significantly associated with a reduction in mosquito infection rates. Further research can help establish causality and contribute to our understanding of P. vivax transmission and vaccine development.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Lindsay B. Stewart, Aline Freville, Till S. Voss, David A. Baker, Gordon A. Awandare, David J. Conway
Summary: Asexual blood-stage malaria parasites must produce sexual progeny for mosquito infection. Two different methods of commitment rate measurement were compared, with higher sensitivity and precision observed in the detection of an early differentiation marker. Clinical isolates and laboratory-adapted lines showed significant variation in commitment rates, and the effects of choline on commitment varied quantitatively.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Lucien Platon, David A. Baker, Didier Menard
Summary: The ring-stage survival assay is the reference assay to measure in vitro Plasmodium falciparum artemisinin partial resistance. The main challenge of the standard protocol is to generate 0-to-3-h postinvasion ring stages (the stage least susceptible to artemisinin) from schizonts obtained by sorbitol treatment and Percoll gradient.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Mehul Sharma, Daniel Leung, Mana Momenilandi, Lauren C. W. Jones, Lucia Pacillo, Alyssa E. James, Jill R. Murrell, Selket Delafontaine, Jesmeen Maimaris, Maryam Vaseghi-Shanjani, Kate L. Del Bel, Henry Y. Lu, Gilbert T. Chua, Silvia Di Cesare, Oriol Fornes, Zhongyi Liu, Gigliola Di Matteo, Maggie P. Fu, Donato Amodio, Issan Yee San Tam, Gavin Shueng Wai Chan, Ashish A. Sharma, Joshua Dalmann, Robin van der Lee, Geraldine Blanchard-Rohner, Susan Lin, Quentin Philippot, Phillip A. Richmond, Jessica J. Lee, Allison Matthews, Michael Seear, Alexandra K. Turvey, Rachael L. Philips, Terri F. Brown-Whitehorn, Christopher J. Gray, Kosuke Izumi, James R. Treat, Kathleen H. Wood, Justin Lack, Asya Khleborodova, Julie E. Niemela, Xingtian Yang, Rui Liang, Lin Kui, Christina Sze Man Wong, Grace Wing Kit Poon, Alexander Hoischen, Caspar I. van der Made, Jing Yang, Koon Wing Chan, Jaime Sou Da Rosa Duque, Pamela Pui Wah Lee, Marco Hok Kung Ho, Brian Hon Yin Chung, Huong Thi Minh Le, Wanling Yang, Pejman Rohani, Ali Fouladvand, Hassan Rokni-Zadeh, Majid Changi-Ashtiani, Mohammad Miryounesi, Anne Puel, Mohammad Shahrooei, Andrea Finocchi, Paolo Rossi, Beatrice Rivalta, Cristina Cifaldi, Antonio Novelli, Chiara Passarelli, Stefania Arasi, Dominique Bullens, Kate Sauer, Tania Claeys, Catherine M. Biggs, Emma C. Morris, Sergio D. Rosenzweig, John J. O'Shea, Wyeth W. Wasserman, H. Melanie Bedford, Clara D. M. van Karnebeek, Paolo Palma, Siobhan O. Burns, Isabelle Meyts, Jean-Laurent Casanova, Jonathan J. Lyons, Nima Parvaneh, Anh Thi Van Nguyen, Caterina Cancrini, Jennifer Heimall, Hanan Ahmed, Margaret L. McKinnon, Yu Lung Lau, Vivien Beziat, Stuart E. Turvey
Summary: Sharma et al. define a new primary atopic disorder caused by heterozygous gain-of-function variants in STAT6. This leads to severe early-onset allergies in 16 patients from 10 families. Treatment with anti-IL-4Rα antibody and JAK inhibitor is highly effective.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Leen N. N. Vanheer, Almahamoudou Mahamar, Emilia Manko, Sidi M. M. Niambele, Koualy Sanogo, Ahamadou Youssouf, Adama Dembele, Makonon Diallo, Seydina O. O. Maguiraga, Jody Phelan, Ashley Osborne, Anton Spadar, Merel J. J. Smit, Teun Bousema, Chris Drakeley, Taane G. G. Clark, William Stone, Alassane Dicko, Susana Campino
Summary: Sequence analysis of Plasmodium falciparum parasites in asymptomatic individuals in South-Western Mali reveals an increase in genetic variation and drug resistance frequencies. This is important for monitoring the emergence and spread of drug-resistant parasites, informing malaria control efforts in Mali and worldwide.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Samantha Donsante, Giulia Siciliano, Mariagrazia Ciardo, Biagio Palmisano, Valeria Messina, Valeria de Turris, Giorgia Farinacci, Marta Serafini, Francesco Silvestrini, Alessandro Corsi, Mara Riminucci, Pietro Alano
Summary: Recent evidence suggests that the bone marrow (BM) plays a key role in the diffusion of P. falciparum malaria by providing a niche for the maturation of the parasite gametocytes, responsible for human-to-mosquito transmission. Suitable humanized in vivo models to study the mechanisms of the interplay between the parasite and the human BM components are still missing. We report a novel experimental system based on the infusion of immature P. falciparum gametocytes into immunocompromised mice carrying chimeric ectopic ossicles whose stromal and bone compartments derive from human osteoprogenitor cells. Our model represents a powerful tool to study BM function and the interplay essential for parasite transmission in P. falciparum malaria and can be extended to study other infections in which the human BM plays a role.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Joeri van Strien, Felix J. Evers, Madhurya Lutikurti, Stijn Berendsen, Alejandro A. Garanto, Geert-Jan van Gemert, Alfredo Cabrera-Orefice, Richard Rodenburg, Ulrich Brandt, Taco W. A. Kooij, Martijn Huynen
Summary: Complexome profiling is a method for large-scale, untargeted, and comprehensive characterization of protein complexes in a biological sample. The Comparative Clustering (CompaCt) approach allows automated integrative analysis of complexome profiling data from multiple species, enabling systematic characterization and comparison of complexomes. This method has been applied to analyze a collection of complexome profiles from different species, leading to the identification of novel candidate interactors and complexes, and shedding light on the evolution of metazoan protein complexes.
PLOS COMPUTATIONAL BIOLOGY
(2023)