Article
Chemistry, Analytical
Ivan Vuckovic, Aleksandar Denic, M. Cristine Charlesworth, Milovan Suvakov, Shane Bobart, John C. Lieske, Fernando C. Fervenza, Slobodan Macura
Summary: Several postprocessing methods for measuring protein concentrations in human urine using existing H-1 nuclear magnetic resonance (NMR) metabolomic spectra were described, including direct spectral integration, integration of different spectra, and matching protein patterns. The study found excellent correlation between NMR integrals and protein content measured by the BCA assay, and indicated that TSP spectral parameters can be good indicators of protein concentration.
ANALYTICAL CHEMISTRY
(2021)
Article
Clinical Neurology
Alireza M. Salehi, Ida A. K. Nilsson, Joao Figueirae, Laura M. Thornton, Israa Abdulkarim, Erik Palsson, Cynthia M. Bulik, Mikael Landen
Summary: The study found that lower serum concentrations of methanol in patients with anorexia nervosa and those who have recovered from the disorder may serve as either a trait marker or a scar effect of anorexia nervosa.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Clinical Neurology
Zachary R. Wanner, Cormac G. Southam, Prachi Sanghavi, Naveenjyote S. Boora, Eric J. Paxman, Sean P. Dukelow, Brian W. Benson, Tony Montina, Gerlinde A. S. Metz, Chantel T. Debert
Summary: This study examined the metabolic changes post-sport-related concussion (SRC) through urine analysis, identifying specific metabolites associated with SRC and suggesting a more objective method for characterizing the condition. The correlations found between specific metabolites and factors like symptom burden and length of return an potential applications as biomarkers for SRC diagnosis and treatment.
FRONTIERS IN NEUROLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Matthew Barden, Marie M. Phelan, Robert Hyde, Alkiviadis Anagnostopoulos, Bethany E. Griffiths, Cherry Bedford, Martin Green, Androniki Psifidi, Georgios Banos, Georgios Oikonomou
Summary: The strongest evidence of being associated with sole lesions corresponded to phenylalanine and 4 unlabeled metabolites. The serum metabolome, as characterized by proton nuclear magnetic resonance spectroscopy, cannot predict the presence or future development of sole lesions. A small number of metabolites may be associated with sole lesions, but they can only explain a small proportion of the differences between affected and unaffected animals due to poor prediction accuracies. Future metabolomic studies should effectively control for interanimal and extraneous sources of spectral variation to reveal underlying metabolic mechanisms of sole lesion etiopathogenesis in dairy cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Chengkeng Tan, Jinap Selamat, Nuzul Noorahya Jambari, Rashidah Sukor, Suganya Murugesu, Azira Muhamad, Alfi Khatib
Summary: This study characterized the metabolic composition of four commercially available chicken breeds using H-1 NMR coupling and multivariate analysis. The results showed that local village chicken could be distinguished from other breeds based on the metabolites present in their serum and pectoralis major muscle. However, the colored broiler (Hubbard) could not be distinguished from broiler (Cobb) and spent layers (Dekalb) in both serum and pectoralis major.
FOOD SCIENCE & NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Panagiota D. Georgiopoulou, Styliani A. Chasapi, Irene Christopoulou, Anastasia Varvarigou, Georgios A. Spyroulias
Summary: Neonatal sepsis is a critical medical condition that occurs after preterm birth, and it is challenging to accurately diagnose or predict the disease. This study aimed to investigate the urine metabolomic profile of septic neonates through nuclear magnetic resonance (NMR) and identify potential biomarkers for distinguishing between healthy and diseased newborns.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Analytical
Ivica Kopriva, Ivanka Jeric, Marijana Popovic Hadzija, Mirko Hadzija, Marijana Vucic Lovrencic
Summary: The proposed method introduces a library-based approach to quantify proportions of overlapping metabolites from H-1 NMR mixture spectra, addressing the challenge of metabolite concentration analysis. By utilizing the linear non-negative least squares (NNLS) problem, the method successfully made progress in differentiating metabolites that discriminate the T2DM group from the control group.
ANALYTICAL CHEMISTRY
(2021)
Article
Obstetrics & Gynecology
Mathilde Bourdon, Pietro Santulli, Fatiha Kateb, Khaled Pocate-Cheriet, Frederic Batteux, Chloe Maignien, Sandrine Chouzenoux, Corinne Bordonne, Louis Marcellin, Gildas Bertho, Charles Chapron
Summary: The study found differences in serum metabolic profiles between patients with adenomyosis and the control group, with specific metabolic profiles observed in different adenomyosis phenotypes. These metabolic changes are associated with processes such as immune activation, cell proliferation, and cell migration.
FERTILITY AND STERILITY
(2021)
Article
Psychiatry
Caigui Lin, Qing Hu, Jiyang Dong, Zhiliang Wei, Jie Li, Zhong Chen
Summary: This study revealed the specific metabolic alterations in patients with both schizophrenia and hepatitis B virus infection, highlighting the synergistic action between the two pathologies. These findings provide important perspectives for designing precise therapies for schizophrenia patients with hepatitis B virus infection.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Endocrinology & Metabolism
James Gerard Wolfsberger, Emily C. Hunt, Sai Sumedha Bobba, Sharifa Love-Rutledge, Bernhard Vogler
Summary: This study describes a pre-acquisition method of urine normalization, which adjusts for differences in the hydration state of each organism and results in a narrower concentration range in a targeted analysis.
Article
Biochemical Research Methods
Banny S. B. Correia, Vinicius G. Ferreira, Priscila M. F. D. Piagge, Mariana B. Almeida, Nilson A. Assuncao, Joyce R. S. Raimundo, Fernando L. A. Fonseca, Emanuel Carrilho, Daniel R. Cardoso
Summary: This study used metabolomics to compare Covid-19 patients with a control group, revealing alterations in metabolite levels and identifying six metabolites that could potentially serve as prognostic markers for the disease. The metabolic changes observed suggest that SARS-CoV-2 infection disrupts the energetic system in the human body, supporting viral replication and correlating with the severity of clinical conditions in patients.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Applied
Shan Huang, Si Ying Lim, Hazel Lau, Wuzhong Ni, Sam Fong Yau Li
Summary: The exogenous application of glycinebetaine (GB) at an appropriate concentration effectively stabilizes the quality-related metabolites of cold-stored strawberries, including sugars and amino acids, while reducing the acid content. Moreover, the GB content in strawberries also increases. These findings suggest that GB is a natural and safe treatment that can maintain the quality of cold-stored strawberries and potentially provide methyl-donor supplementation in our daily diet.
Article
Biophysics
Wei Ye, Yan Lin, Tedros Bezabeh, Changchun Ma, Jiahao Liang, Jiayun Zhao, Ting Ouyang, Wan Tang, Renhua Wu
Summary: The study compared the metabolites of esophageal cancer patients and healthy controls in serum and tissues, showing distinct differences in metabolic profiles. Metabolic alterations in tissue and serum levels were observed in EC patients, with overlapping discriminatory metabolites including valine, alanine, and glucose. Perturbation of pyruvate metabolism was strongly associated with EC progression, and acetate and pyruvate were identified as potential serum biomarkers for EC.
NMR IN BIOMEDICINE
(2021)
Article
Oncology
Junfei Zhang, Yuanxin Du, Yongcai Zhang, Yanan Xu, Yanying Fan, Yan Li
Summary: In this study, the metabonomic profiling of serum samples of lung metastasis mice with colorectal cancer was analyzed using 1H-nuclear magnetic resonance (1H-NMR). The results showed distinct differences between the metastatic mice and control mice based on their metabolic profiles at the serum level. The study also identified several metabolites that were closely associated with the lung metastasis process. Pathway enrichment analysis revealed deregulation of metabolic pathways involved in the lung metastasis process. This study provides potential diagnostic biomarkers for the early diagnosis of lung metastasis in colorectal cancer.
CANCER MANAGEMENT AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Domitilla Vanni, Nicola Viceconte, Greta Petrella, Flavio Giuseppe Biccire, Francesco Pelliccia, Gaetano Tanzilli, Daniel Oscar Cicero
Summary: Takotsubo syndrome (TTS) is characterized by acute coronary syndrome with severe left ventricular dysfunction and non-obstructive coronary artery disease, mainly caused by beta-adrenergic overstimulation and acute endogenous catecholamine surge. Studies have shown evidence of inflammation and oxidative stress overload in myocardial tissue of TTS models, but systemic biochemical evidence of augmented oxidant activity in patients with TTS is lacking. The systemic metabolic profile of TTS patients showed significant elevation of ketone bodies, 2-hydroxybutyrate, acetyl-L-carnitine, and glutamate levels, reflecting a systemic response to oxidative stress caused by the stressor that triggered the syndrome's onset.
Editorial Material
Peripheral Vascular Disease
Miroslaw Banasik, Tomasz Dawiskiba, Krzysztof Letachowicz, Slawomir Zmonarski, Oktawia Mazanowska, Edyta Lipiak, Marcin Mis, Dariusz Janczak, Magdalena Krajewska
Summary: In this case, a 52-year-old woman developed unilateral pleural effusion and massive edema of the upper limb, breast, and face after renal transplantation. Extensive vein occlusion and high venous flow from arteriovenous fistula were identified as the causes of impaired venous drainage, with fistula ligation and blood flow reduction ultimately resolving the issues. This case highlights the importance of careful consideration and multiple treatment attempts in managing complications after renal transplantation.
JOURNAL OF VASCULAR ACCESS
(2021)
Article
Cardiac & Cardiovascular Systems
Piotr Barc, Maciej Antkiewicz, Barbara sliwa, Katarzyna Fraczkowska, Maciej Guzinski, Tomasz Dawiskiba, Malgorzata Malodobra-Mazur, Wojciech Witkiewicz, Diana Kupczynska, Bartlomiej Strzelec, Dariusz Janczak, Jan Pawel Skora
Summary: Gene therapy with VEGF/HGF shows significant efficacy in patients with CLI complicated by diabetes, leading to increased serum levels of VEGF, improved ankle-brachial index, reduced rest pain, and enhanced vascularization.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2021)
Article
Endocrinology & Metabolism
Aneta Cierzniak, Dorota Pawelka, Krzysztof Kaliszewski, Jerzy Rudnicki, Tadeusz Dobosz, Malgorzata Malodobra-Mazur
Summary: By analyzing gene expression levels and DNA methylation in patients with obesity and insulin resistance, it was found that obese patients with insulin resistance have hypermethylation in gene promoters, with methylation rates negatively correlated with gene expression, suggesting excessive methylation as an epigenetic pathway linking obesity to subsequent insulin resistance.
INTERNATIONAL JOURNAL OF OBESITY
(2021)
Article
Medicine, General & Internal
Katarzyna Nowanska, Miroslaw Banasik, Piotr Donizy, Katarzyna Koscielska-Kasprzak, Slawomir Zmonarski, Krzysztof Letachowicz, Dorota Kaminska, Oktawia Mazanowska, Hanna Augustyniak-Bartosik, Andrzej Tukiendorf, Anna Chudiak, Tomasz Dawiskiba, Agnieszka Halon, Magdalena Krajewska
Summary: The study evaluated the expression of ETA receptors in glomeruli of renal transplant patients, finding positive staining in 8.7% of patients. Patients with positive ETA receptor expression in glomeruli were more likely to develop antibody-mediated rejection (AMR), with a higher graft loss rate in the first year after biopsy compared to patients without ETA receptor expression in glomeruli.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Maciej Antkiewicz, Wiktor Kuliczkowski, Marcin Protasiewicz, Magdalena Kobielarz, Piotr Barc, Maciej Malinowski, Katarzyna Fraczkowska, Katarzyna Kulikowska, Marcin Merenda, Krzysztof Jacyna, Tomasz Dawiskiba, Dariusz Janczak
Summary: This study evaluated a new method for measuring invasive pressure inside abdominal aortic aneurysms during EVAR, finding that it is safe and feasible. The results showed significant differences in pressure values between the aneurysm sac and aorta, suggesting potential implications for predicting early endoleak occurrences.
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Bogunia-Kubik, Wojciech Wojtowicz, Jerzy Swierkot, Karolina Anna Mielko, Badr Qasem, Joanna Wielinska, Renata Sokolik, Lukasz Pruss, Piotr Mlynarz
Summary: RA, AS, and PsA are comprehensive immunological disorders with treatment focused on symptom relief. Metabolite changes were most significant in AS, with the shortest time to remission, potentially influenced by ethanol as a common metabolite negatively correlated with healing processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Tomasz Dawiskiba, Wojciech Wojtowicz, Badr Qasem, Marceli Lukaszewski, Karolina Anna Mielko, Agnieszka Dawiskiba, Miroslaw Banasik, Jan Pawel Skora, Dariusz Janczak, Piotr Mlynarz
Summary: The study demonstrated the potential of serum metabolomic analysis as a tool for distinguishing between coma patients and individuals with brain death, providing valuable insights into the pathogenic mechanisms underlying these conditions.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Alicja Warowicka, Badr Qasem, Anna Dera-Szymanowska, Maria Wolun-Cholewa, Patryk Florczak, Nikodem Horst, Marta Napierala, Krzysztof Szymanowski, Lukasz Popenda, Grazyna Bartkowiak, Ewa Florek, Anna Gozdzicka-Jozefiak, Piotr Mlynarz
Summary: The study found that protoberberine compounds from Chelidonium majus effectively prevent the recurrence of endometriosis in rats. Metabolomics analysis revealed significant changes in metabolite concentrations related to energy homeostasis after treatment. This suggests that natural protoberberine plant compounds have pharmaceutical activity and further research should focus on understanding their mechanisms of action.
Article
Chemistry, Multidisciplinary
Karolina Anna Mielko, Natalia Pudelko-Malik, Aneta Tarczewska, Piotr Mlynarz
Summary: Nuclear magnetic resonance (NMR) is a key method in metabolomics and structural analysis, with high demand for solvents that can be simplified for biological studies with D2O and H2O, but more complex metabolomics studies require MeOH or MeCN. The use of NMR as an eco-friendly method aligns with solvent sustainability guidelines.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemistry & Molecular Biology
Karolina Anna Mielko, Slawomir Jan Jablonski, Lukasz Pruss, Justyna Milczewska, Dorota Sands, Marcin Lukaszewicz, Piotr Mlynarz
Summary: The research compared the metabolisms of two P. aeruginosa strains, showing that the differences between drug-resistant and drug-susceptible strains mainly lie in the turnover of amino acids. This study provides valuable information to complement research on drug resistance in P. aeruginosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Karolina Anna Mielko, Slawomir Jan Jablonski, Marcin Lukaszewicz, Piotr Mlynarz
Summary: Metabolic analyses of six bacterial strains were conducted with three cell disintegration methods, showing significant influence on metabolite concentrations and differences among strains. NMR could be useful for bacterial strain identification, and regardless of the disintegration method, strain groups could be identified through multivariate data analysis.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Ewelina Wanarska, Karolina Anna Mielko, Irena Maliszewska, Piotr Mlynarz
Summary: This article describes the use of antimicrobial photodynamic inactivation as a non-antibiotic alternative method to inactivate Acinetobacter baumannii. The study found that phototherapy effectively reduced the number of viable cells and had no significant impact on biofilm formation and antibiotic resistance.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mateusz Glod, Dominik Marciniak, Krzysztof Kaliszewski, Krzysztof Sutkowski, Jerzy Rudnicki, Marek Bolanowski, Beata Wojtczak
Summary: Risk factors for phonation disorders after thyroidectomy are influenced by various factors, with retrosternal goiter and volume above 100mL being the most important risk factors. Other factors such as having thyroid cancer, being a male over 65 years old with obesity, undergoing secondary operations, and only having visual RLN identification also increase the risk of phonation disorders.
Article
Nutrition & Dietetics
Ewa Stachowska, Dominika Maciejewska-Markiewicz, Joanna Palma, Karolina Anna Mielko, Badr Qasem, Katarzyna Kozlowska-Petriczko, Marcin Ufnal, Katarzyna Ewa Sokolowska, Victoria Hawrylkowicz, Patrycja Zaleska, Karolina Jakubczyk, Ewa Wunsch, Karina Ryterska, Karolina Skonieczna-Zydecka, Piotr Mlynarz
Summary: Replacing bread with high-fiber buns can beneficially change the metabolome of NAFLD patients, reducing liver steatosis.
