Article
Environmental Sciences
Bin Wang, Lei Song, Wenjia Li, Li'an Hou, Jiang Li, Xiaoyi Xu, Guishang Sheng
Summary: The prevalence and correlation of antibiotic resistance genes (ARGs) in pig farm wastewater treatment plants (WWTPs) and surrounding environment were investigated using metagenomics and q-PCR. The abundance of ARGs decreased significantly in the A/O process and disinfection tank of WWTPs. However, new ARGs emerged in wastewater that passed though the anaerobic reactor. The abundances of ARGs in the soils and water near pig farm were much higher than those in the control, and the phyla Proteobacteria, Choroflexi, and Actinobacteriota dominated the water and soil samples.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Dong Ding, Jingyuan Zhu, Yanling Gao, Fan Yang, Yan Ma, Xuemin Cheng, Jinlei Li, Peng Dong, Haiyan Yang, Shuaiyin Chen
Summary: Livestock farms are recognized as the main sources of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) with potential implications for human health. This study found that exposure to the farm environment may have remodeled farmworkers' oropharynx and gut microbiota, reduced microbial diversity, and enriched some opportunistic pathogenic bacteria. Compared with villagers, ARG abundance in the oropharynx of farmworkers increased significantly, suggesting that farm exposure may have affected the microbial composition and increased ARG abundance of farmworkers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Cuihong Tong, Danyu Xiao, Longfei Xie, Jintao Yang, Ruonan Zhao, Jie Hao, Zhipeng Huo, Zhenling Zeng, Wenguang Xiong
Summary: The study revealed a high richness and diversity of ARGs in swine manure, with inactivating tetracycline resistance genes being prevalent. Workers and the environment were identified as primary recipients of ARGs, with shared ARGs accounting for at least 90% of their ARG abundances. Network analysis showed Escherichia, Acinetobacter, and Erysipelothrix as the most dominant genera co-occurring with specific shared ARGs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Longkai Qiao, Xiaoxia Liu, Shuo Zhang, Luying Zhang, Xianhong Li, Xuesong Hu, Qiancheng Zhao, Qingyu Wang, Caihong Yu
Summary: The study found that arsenic, lead, and cadmium were the main pollutants in the farmland surrounding gold tailings. Proteobacteria and Actinobacteria were the dominant phyla of microbes in the area. A total of 75 different ARGs were detected in the soil samples, with macrolide-, lincosaminide-, and streptogramin B resistant genes being the majority.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Qiqi Fu, Zhiqiang Chen, Zhigang Yu, Yiqi Wu, Huanyu Bao, Jianhua Guo, Qinxue Wen
Summary: This study analyzed the variations of intracellular antibiotic resistance genes (iARGs) and extracellular antibiotic resistance genes (eARGs) in swine manure composting. The abundance of ARGs rebounded from the thermophilic phase to the mature phase due to the release of persistent eARGs and the increase of certain iARGs during cell proliferation. The conjugative transfer frequencies of ARGs in composting communities were comparable to pre-composting frequency, and ozone treatment was optimized to suppress the rebound and spread of ARGs, resulting in significant reduction of iARGs and decreased possibilities of conjugation and transformation among the composting products.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agriculture, Dairy & Animal Science
Barbara Salerno, Maddalena Furlan, Raffaella Sabatino, Andrea Di Cesare, Marta Leati, Marcello Volanti, Lisa Barco, Massimiliano Orsini, Carmen Losasso, Veronica Cibin
Summary: This study analyzed a broiler farm that has never used antibiotics and found that not using antibiotics does not fully reduce the load of antibiotic resistance genes (ARGs) in meat processing environments. In addition, other sources of ARGs, including the production phase and breeding stage, should be considered.
Article
Environmental Sciences
Xiangyue Xu, Wenjin Ma, Kaixiang Zhou, Boyu An, Meixia Huo, Xudong Lin, Lei Wang, Hanyu Wang, Zhenli Liu, Guyue Cheng, Lingli Huang
Summary: Aerobic composting effectively removes doxycycline and antibiotic resistance genes from animal manure, with a removal rate of over 97%. The microbial diversity and abundance of antibiotic resistance genes are higher in composted swine manure compared to composted broiler manure. The composting process leads to changes in microbial community structure and an increase in multidrug resistance genes and mobile genetic elements.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Huan He, Mingzhi Yang, Wentao Li, Zeqing Lu, Yizhen Wang, Mingliang Jin
Summary: This study investigated the dynamic changes of porcine fecal microbiota and metabolites at different stages from birth to market, providing a reference for understanding the intestinal health status of pigs at different growth stages.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Okugbe Ebiotubo Ohore, Yuwen Wang, Yunjie Wei, Edmond Sanganyado, Muhammad Shafiq, Xiaoyang Jiao, Wenhua Liu, Amechi S. Nwankwegu, Zhen Wang
Summary: This study investigated the molecular mechanisms of phylogenetic turnover in microbial communities and the impact of antibiotics and antibiotic resistance genes (ARGs) on microbial assemblages. The research found that increased concentrations of antibiotics in ecosystems can stimulate the proliferation of ARGs, potentially affecting the assembly of microbial communities. Additionally, the study revealed complex interrelationships between ARGs and microbial communities, with arthropods and nematodes showing the strongest potential for acquiring ARGs.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Infectious Diseases
Jenna Schueler, Kayla Naas, Jerod Hurst, Diana Aga, Stephanie Lansing
Summary: This study found that antibiotics were not completely degraded during farm-scale composting, potentially impacting nitrogen transformation in the compost. Total Kjeldahl nitrogen content increased during composting, likely due to the inhibition of N-mineralization by antibiotics.
Article
Environmental Sciences
Jose Gustavo Venancio da Silva Ramos, Camila Palacio Richter, Maria Alice Silva, Giordana Longo Singolano, Gabriel Hauagge, Eduarda Lorencon, Ismael Laurindo Costa Junior, Thiago Edwiges, Priscila Vaz de Arruda, Carlos Magno de Sousa Vidal
Summary: This study investigated the influence of adding ciprofloxacin on the anaerobic digestion of swine wastewater. The results showed that the optimal volumetric organic loading rate for the reactor was 0.60 +/- 0.11 gSV L-1 d(-1), resulting in a biogas productivity of 0.51 +/- 0.03 L-biogas L-1 d(-1). Adding 0.5 mgCIP L-1 substrate resulted in a significant drop in productivity from the 7th to 11th day, followed by recovery and stability.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Environmental Sciences
Qihe Tang, Qianwen Sui, Yuansong Wei, Peihong Shen, Junya Zhang
Summary: Composting is an effective strategy for reducing antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) in animal manure. This study examined the fate of ARB and ARGs after applying swine-manure composts (SMCs) to different soil types. The results showed that SMCs initially increased total ARG abundance in the soil but significantly reduced it after 82 days. The SMCs introduced specific ARGs into the soils, which declined over time. However, the presence of SMCs increased the number of ARB in the soils, especially cefotaxime-resistant bacteria. The microbiome also took longer to recover compared to the control. Statistical analysis suggested that changes in the microbial community were the main factor influencing the fate of ARGs during SMCs land application.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Environmental Sciences
Yu Zhang, Yunhao Zheng, Zhiping Zhu, Yongxing Chen, Hongmin Dong
Summary: This study found that most of the ARG subtypes detected in biogas digestate could be dispersed into the atmosphere through aerosol dispersion, with different relative abundance and diversity of ARGs in biogas digestate compared to aerosols, and no significant correlation between the relative abundance of ARGs in biogas digestate and aerosols. Storage of biogas digestate led to an increase in abundance of certain ARG subtypes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Wenjin Ma, Lei Wang, Xiangyue Xu, Meixia Huo, Kaixiang Zhou, Kun Mi, Xiaoyuan Tian, Guyue Cheng, Lingli Huang
Summary: Livestock manure is a significant source of antibiotic resistance genes (ARGs) that spread to the environment, potentially endangering human health. This study found that florfenicol (FF) and thiamphenicol (TAP) dissipate rapidly during composting, but cannot be completely removed, leading to negative impacts on bacterial communities, mobile genetic elements (MGEs), and ARGs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Chengyu Liang, Dong Wei, Siying Zhang, Qiuhui Ren, Jiping Shi, Li Liu
Summary: The study used membrane filtration to treat swine wastewater, reducing conventional pollutants and ARGs, thus decreasing the risk of ARGs entering natural water bodies. Results showed that removal of ARGs was correlated with total phosphorus, suspended solids, and electrical conductivity in the wastewater.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Microbiology
Liang-Xing Fang, Chong Chen, Dong-Ling Yu, Ruan-Yang Sun, Chao-Yue Cui, Liang Chen, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Letter
Infectious Diseases
Chong Chen, Liang Chen, Yan Zhang, Chao-Yue Cui, Xiao-Ting Wu, Qian He, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Letter
Microbiology
Chong Chen, Xiao-Ting Wu, Qian He, Liang Chen, Chao-Yue Cui, Yan Zhang, Shao-Hua Chen, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Microbiology
Chao-Yue Cui, Chong Chen, Bao-Tao Liu, Qian He, Xiao-Ting Wu, Ruan-Yang Sun, Yan Zhang, Ze-Hua Cui, Wen-Ying Guo, Qiu-lin Jia, Cang Li, Barry N. Kreiswirth, Xiao-Ping Liao, Liang Chen, Ya-Hong Liu, Jian Sun
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Infectious Diseases
Ze-Hua Cui, Wei-Na Ni, Tian Tang, Bing He, Zi-Xing Zhong, Liang-Xing Fang, Liang Chen, Chong Chen, Chao-Yue Cui, Ya-Hong Liu, Xiao-Ping Liao, Jian Sun
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Review
Biochemistry & Molecular Biology
Liang-Xing Fang, Chong Chen, Chao-Yue Cui, Xing-Ping Li, Yan Zhang, Xiao-Ping Liao, Jian Sun, Ya-Hong Liu
Article
Immunology
Ze-Hua Cui, Zi-Jian Zheng, Tian Tang, Zi-Xing Zhong, Chao-Yue Cui, Xin-Lei Lian, Liang-Xing Fang, Qian He, Xi-Ran Wang, Chong Chen, Bing He, Min-Ge Wang, Ya-Hong Liu, Xiao-Ping Liao, Jian Sun
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Genetics & Heredity
Chong Chen, Chao-Yue Cui, Jun-Jun Yu, Qian He, Xiao-Ting Wu, Yu-Zhang He, Ze-Hua Cui, Cang Li, Qiu-Lin Jia, Xiang-Guang Shen, Ruan-Yang Sun, Xi-Ran Wang, Min-Ge Wang, Tian Tang, Yan Zhang, Xiao-Ping Liao, Barry N. Kreiswirth, Shi-Dan Zhou, Bin Huang, Hong Du, Jian Sun, Liang Chen, Ya-Hong Liu
Article
Microbiology
Chong Chen, Chao-Yue Cui, Xiao-Ting Wu, Liang-Xing Fang, Qian He, Bing He, Teng-Fei Long, Xiao-Ping Liao, Liang Chen, Ya-Hong Liu, Jian Sun
Summary: The recent discovery of plasmid-mediated tigecycline-resistant gene tet(X) in sick ducks and chickens in Guangdong, China, challenges the clinical effectiveness of tigecycline. The isolates exhibited high degradation activities on tetracyclines by hydroxylation and showed multidrug resistance to several antibiotics. Additional efforts are needed to evaluate the clinical impact of these tigecycline resistance genes.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Chao-Yue Cui, Qian He, Qiu-Lin Jia, Cang Li, Chong Chen, Xiao-Ting Wu, Xiao-Jing Zhang, Zhuo-Yu Lin, Zi-Jian Zheng, Xiao-Ping Liao, Barry N. Kreiswirth, Ya-Hong Liu, Liang Chen, Jian Sun
Summary: This study identified key residue changes that enhance Tet(X) activity and highlighted their crucial role in the molecular evolution of Tet(X) orthologs. These mutations indirectly affect enzyme activity by altering the conformational dynamics and provide insights into the evolutionary relationship between Tet(X) orthologs.
Article
Microbiology
Yang Yu, Chao-Yue Cui, Xu Kuang, Chong Chen, Min-Ge Wang, Xiao-Ping Liao, Jian Sun, Ya-Hong Liu
Summary: This study identified and characterized the prevalence of tet(X4) resistance gene in E. coli isolates from duck and goose farms in Southeast China. The presence of tet(X4) raises concerns about the widespread tigecycline resistance in animal farming and highlights the need for surveillance of this primary resistance gene.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Chao-Yue Cui, Xiao-Jie Li, Chong Chen, Xiao-Ting Wu, Qian He, Qiu-Lin Jia, Xiao-Jing Zhang, Zhuo-Yu Lin, Cang Li, Liang-Xing Fang, Xiao-Ping Liao, Ya-Hong Liu, Bo Hu, Jian Sun
Summary: The emergence of novel plasmid-mediated high-level tigecycline resistance genes tet(X) in Enterobacteriaceae poses a public health risk. A comprehensive analysis of tet(X4)-positive Escherichia coli isolates from a hospital in Guangdong province, China revealed genetic diversity among these isolates, with certain strains showing clonal transmission. Most tet(X4) genes were located on conjugative plasmids and coexisted with other antibiotic resistance genes in patient isolates. The IncX1 plasmid type was a common vector for tet(X4) and demonstrated strong transmission ability and wide host range. Phylogenetic analysis indicated potential cross-sectorial clonal transmission between human and animal-derived tet(X4)-positive E. coli isolates.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Chong Chen, Ping-Yu Huang, Chao-Yue Cui, Qian He, Jian Sun, Ya-Hong Liu, Jin-Lin Huang
Summary: This study classified the plasmid-mediated tet(X) genes in Acinetobacter species and analyzed their structural characteristics, transferability, and in vivo effects. The results revealed the widespread distribution of these plasmids and their potential to transfer antibiotic resistance. They pose a significant threat to the clinical effectiveness of tigecycline.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Medicine, Research & Experimental
Chao-Yue Cui, Qiwei Chen, Qian He, Chong Chen, Rong-Min Zhang, Youjun Feng, Jian Sun
Summary: Tetracycline and its derivative tigecycline are clinical options against Gram-negative bacterial infections, but the emergence of mobile Tet(X) enzymes poses a challenge to antibacterial therapy and food/environmental security. This review presents various Tet(X) variants, discussing their structure, action, evolutional origin, and potential inhibitors to improve understanding of Tet(X)-mediated tigecycline resistance.
WIRES MECHANISMS OF DISEASE
(2022)
Article
Immunology
Chong Chen, Chao-Yue Cui, Yan Zhang, Qian He, Xiao-Ting Wu, Gong Li, Xiao-Ping Liao, Barry N. Kreiswirth, Ya-Hong Liu, Liang Chen, Jian Sun
EMERGING MICROBES & INFECTIONS
(2019)
