Article
Biochemistry & Molecular Biology
Vanna Denti, Allia Mahajneh, Giulia Capitoli, Francesca Clerici, Isabella Piga, Lisa Pagani, Clizia Chinello, Maddalena Maria Bolognesi, Giuseppe Paglia, Stefania Galimberti, Fulvio Magni, Andrew Smith
Summary: Predicting the prognosis of colorectal cancer patients is challenging, but characterizing the tumor immune environment is crucial. This study explored using spatial lipidomics by MALDI-MSI to distinguish CRC tissue based on TIL content, successfully identifying three lipid signals with the greatest impact on differentiation. Overall, the study suggests that lipidomics MALDI-MSI could be a promising tool for subtyping the diverse immune environments in CRC.
Article
Chemistry, Analytical
Xing Li, Tuo Li, Zhenpeng Wang, Jinchao Wei, Jianan Liu, Yangyang Zhang, Zhenwen Zhao
Summary: The study revealed that PFOS mainly accumulates in the liver of animals and can cross the blood-brain barrier to cause neurotoxicity. The impact of PFOS is mainly concentrated on glycerophospholipid and sphingolipid metabolism. Additionally, the synthesis of phosphatidylcholine may serve as a self-protection mechanism in response to PFOS-induced inflammation.
Article
Biology
Kerlijne Moorkens, Jo L. M. R. Leroy, Jusal Quanico, Geert Baggerman, Waleed F. A. Marei
Summary: This study investigated the changes in lipid metabolism in the oviduct of mice fed an obesogenic diet. The results showed that the obesogenic diet led to an increase in the overall lipid levels and differential regulation of specific lipids in the oviductal epithelium. These lipidomic changes may impact reproductive events in the oviduct, such as fertilization and early embryo development.
Article
Radiology, Nuclear Medicine & Medical Imaging
Na Sun, Marija Trajkovic-Arsic, Fengxia Li, Yin Wu, Corinna Muench, Thomas Kunzke, Annette Feuchtinger, Katja Steiger, Anna Melissa Schlitter, Wilko Weichert, Irene Esposito, Jens T. Siveke, Axel Walch
Summary: The study demonstrates differences in glycan expression patterns between stromal and cancer cell compartments in PDAC patients, which are significantly associated with patient outcomes. Specific glycans expressed in cancer cell and stromal regions serve as independent prognostic indicators for patient survival.
Article
Microbiology
Wenhao Tang, Joanne Osborne, Laurent Dortet, Gerald Larrouy-Maumus
Summary: In this study, a MALDI-TOF mass spectrometry-based whole cell lipidomic assay was used to identify compounds targeting polymyxin-resistant bacteria. 8 compounds were found to decrease a specific lipid modification associated with resistance in the bacteria.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Oncology
Ahmad Ameri, Pooya Ameri, Nazanin Rahnama, Majid Mokhtari, Meghdad Sedaghat, Fahimeh Hadavand, Rama Bozorgmehr, Mehrdad Haghighi, Farzad Taghizadeh-Hesary
Summary: Low-dose whole-lung irradiation (LD-WLI) with a single fraction of 0.5 Gy or 1 Gy showed feasibility in treating COVID-19 pneumonia. The study demonstrated a response rate of 63.6% and clinical recovery rate of 55.5% among patients who received the treatment. Additional randomized trials without radiation are needed to assess the efficacy of LD-WLI for COVID-19.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Review
Radiology, Nuclear Medicine & Medical Imaging
J. Gomes Carvalho, J. Sousa, C. Fernandes, M. Franca
Summary: This article reviews the different types of calcified lesions found in the chest, focusing on lesions outside the lung parenchyma. A location-based approach to the differential diagnosis is used, while discussing the clinical importance of the different types of calcifications.
Article
Oncology
Massimo E. Abate, Silvia Cammelli, Letizia Ronchi, Barbara Diletto, Lorenza Gandola, Anna Paioli, Alessandra Longhi, Emanuela Palmerini, Nadia Puma, Angela Tamburini, Maurizio Mascarin, Elisa Coassin, Arcangelo Prete, Sebastian D. Asaftei, Carla Manzitti, Gianni Bisogno, Marta Pierobon, Luca Coccoli, Mariella Capasso, Giovanni Grignani, Giuseppe M. Milano, Valentina Kiren, Franca Fagioli, Stefano Ferrari, Piero Picci, Elisa Carretta, Roberto Luksch
Summary: This study analyzed the toxicity and outcome predictors in Ewing sarcoma patients with lung metastases treated with high-dose chemotherapy followed by whole-lung irradiation. The results indicated that this combined treatment strategy is feasible and may help in controlling the disease. Further research is needed to explore treatment stratification based on the histological response of the primary tumor.
Review
Oncology
Kasra Kolahdouzan, Mohammadreza Chavoshi, Reyhaneh Bayani, Nima Mousavi Darzikolaee
Summary: For COVID-19 patients, whole lung irradiation (WLI) has shown a mild improvement in reducing intubation days but has no significant effect on overall survival. Routine use of WLI for moderate-to-severe COVID-19 patients cannot be recommended based on the current evidence.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2022)
Article
Oncology
Cindy S. Ortiz, Diana Hernandez, Cesar Trujillo, David Calderon, Placido Esqueda, Flavio Calva, Arcadio Betancourt, Miriam Ramirez, Graciela Cervantes, Miguel A. Souto, Juan G. Celis, Luis R. Nolazco, Alejandro Olmos
Summary: This is a paired prospective comparative cohort study involving 58 patients, aiming to analyze the clinical LD-WLI in patients with moderate or severe COVID19 pneumonia. The results suggest that radiotherapy may be an option to improve the clinical response in patients with COVID-19.
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Biology
Claude J. Rogers, Espoir M. Kyubwa, Agnes Lukaszewicz, Jason Yamada-Hanff, Mark A. Starbird, Thomas A. Miller, Asa A. Phelps, Seth Wallack, Saikanth Mahendra, Karla Thrall, Naresh Menon
Summary: Thoracic exposure to ionizing radiation can result in delayed injuries to the heart and lungs, which are difficult to predict. This study found that changes in the circulating miRNA profile within the first 30 days post-irradiation could predict survival outcomes. The research suggests that plasma miRNA profiles may be used as biomarkers to identify individuals at high risk of organ-specific late radiation injury.
RADIATION RESEARCH
(2021)
Article
Oncology
Govindaraj Ganesan, Sasipriya Ponniah, Vivek Sundaram, Praveen Kumar Marimuthu, Venkatraman Pitchaikannu, Manikandan Chandrasekaran, Janakiraman Thangarasu, Gunasekaran Kannupaiyan, Prabhu Ramamoorthy, Brindha Thangaraj, Harshavardhanan Sasipriya Govindaraj, Shree Vaishnavi Raguram
Summary: The combination of low dose radiotherapy (LDRT) and standard pharmacologic therapy in COVID-19 patients has been shown to improve oxygenation, expedite clinical recovery, shorten hospital stay, and enhance radiological resolution. LDRT's anti-inflammatory effect may provide additional clinical benefit to COVID-19 patients.
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Oncology
Xue Sha, Jinghao Duan, Xiutong Lin, Jian Zhu, Ruohui Zhang, Tao Sun, Hui Wang, Xiangjuan Meng, Yong Yin
Summary: This study aimed to compare the cardiac sparing advantages between photon and proton therapy during whole lung irradiation in pediatric patients with lung metastases. The results showed that the proposed new proton solution, IMPT-2, demonstrated significant superiority in cardiac sparing compared to other techniques, providing maximum dose sparing for the heart and its substructures.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Takamasa Hotta, Takae Okuno, Mika Nakao, Yoshihiro Amano, Takeshi Isobe, Yukari Tsubata
Summary: The emergence of immune checkpoint inhibitors has brought more attention to the abscopal effect. This case report highlights an extracranial abscopal effect after whole-brain irradiation therapy for brain metastasis, with a confirmed reproducibility of the effect. However, the abscopal effect may not be observed in metastatic lesions with low immunogenicity, even within the same patient.
Article
Radiology, Nuclear Medicine & Medical Imaging
Loig Duverge, Charlotte Demoor-Goldschmidt, Anne Laprie, Mathilde Cervellera, Marie Pierre Castex, Nadege Corradini, Perrine Marec-Berard, Line Claude
Summary: This study evaluated the efficacy and safety of Bu-Mel-based high-dose chemotherapy and whole lung irradiation in Ewing sarcoma lung relapses. The results showed favorable long-term survival outcomes in patients with lung metastatic relapses, but the risk of lung toxicity should be considered.
BRITISH JOURNAL OF RADIOLOGY
(2022)
Article
Multidisciplinary Sciences
Courtney E. Chandler, Erin M. Harberts, Mark R. Pelletier, Iyarit Thaipisuttikul, Jace W. Jones, Adeline M. Hajjar, Jason W. Sahl, David R. Goodlett, Aaron C. Pride, David A. Rasko, M. Stephen Trent, Russell E. Bishop, Robert K. Ernst
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell & Tissue Engineering
Manasa P. Srikanth, Jace W. Jones, Maureen Kane, Ola Awad, Tea Soon Park, Elias T. Zambidis, Ricardo A. Feldman
Summary: Gaucher disease is caused by mutations in the GBA1 gene, leading to the accumulation of GlcSph which activates an mTORC1-dependent pathogenic mechanism in neurons. Inhibition of acid ceramidase can prevent mTOR hyperactivity and lysosomal dysfunction, suggesting a potential therapeutic target for GBA1-associated neurodegeneration. Incubation with exogenous GlcSph in wild-type neurons can recapitulate the abnormalities observed in mutant neurons, highlighting the role of GlcSph in the pathogenesis of Gaucher disease.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemical Research Methods
Anh Tran, I. Abrrey Monreal, Eugene Moskovets, Hector C. Aguilar, Jace W. Jones
Summary: An unmet need exists for analytical strategies that can characterize the lipid composition of viral envelopes and do so on a high-throughput scale. The use of atmospheric pressure MALDI with lithium adduct consolidation allows for rapid and confident profiling of total lipid extracts from enveloped viruses, specifically geared towards sphingolipid detection. This approach provides a robust and high-throughput lipid detection platform for structural characterization of lipid extracts from viruses.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Medicine, Research & Experimental
Ni Yang, Lauren E. Parker, Jianshi Yu, Jace W. Jones, Ting Liu, Kyriakos N. Papanicolaou, C. Conover Talbot, Kenneth B. Margulies, Brian O'Rourke, Maureen A. Kane, D. Brian Foster
Summary: The study showed that there is a decline in cardiac ATRA in heart failure, despite sufficient reserves of cardiac vitamin A. Strategies to normalize ATRA metabolism, such as CYP26 inhibition, may have therapeutic potential in preventing cardiac remodeling and functional decline. This suggests that low cardiac ATRA attenuates critical gene expression programs in heart failure.
Article
Chemistry, Analytical
Yulemni Morel, Nivedita Hegdekar, Chinmoy Sarkar, Marta M. Lipinski, Maureen A. Kane, Jace W. Jones
Summary: The study developed a targeted, quantitative method using HILIC separation and selective reaction monitoring mass spectrometry for specific and sensitive quantitation of 100 PE-P structures. Application of this assay to a TBI mouse model demonstrated distinct temporal profiles for plasma PE-P up to 28 days post injury, showing a significant increase 24 hours after induced TBI followed by a gradual reduction to sham concentrations by day 28.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Carballal, Victor Vitvitsky, Roshan Kumar, David A. Hanna, Marouane Libiad, Aditi Gupta, Jace W. Jones, Ruma Banerjee
Summary: This study demonstrates that H2S increases lipid synthesis in cells by affecting mitochondrial NAD(P)H pools and enabling reductive carboxylation of alpha-ketoglutarate. H2S also leads to time-dependent changes in various lipid classes, upregulating triglycerides while downregulating phosphatidylcholine.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Ludovic Muller, Weiliang Huang, Jace W. Jones, Ann M. Farese, Thomas J. MacVittie, Maureen A. Kane
Summary: Radiation exposure leads to dysregulation of lipid and protein composition in the mesenteric lymph node (MLN), affecting pathways regulating lipids and acute phase response. The study demonstrates molecular alterations in MLN after acute radiation exposure, potentially informing on injury mechanism in a non-human primate model of acute radiation syndrome of the gastrointestinal tract.
Article
Biochemistry & Molecular Biology
Owen B. Becette, Anh Tran, Jace W. Jones, John P. Marino, Robert G. Brinson
Summary: Nucleic acids are a popular platform for developing biotherapeutics. There are currently 14 short oligonucleotide therapeutics and 2 mRNA vaccines approved by the FDA. However, there is a lack of high-resolution structural techniques for characterizing these molecules. In this study, nuclear magnetic resonance (NMR) methods were used to structurally fingerprint short oligonucleotide therapeutics, demonstrating their utility and broad applicability.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Cell Biology
Jianshi Yu, Mariarita Perri, Jace W. Jones, Keely Pierzchalski, Natalia Ceaicovscaia, Erika Cione, Maureen A. Kane
Summary: The study investigated the relationship between RBP1 expression and atRA, finding that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Increasing atRA levels could activate direct atRA targets, inhibit proliferation, and collagen deposition. Conditions in tumor microenvironments reduced RBP1 expression and atRA, while treatments with specific agonists or agents could restore RBP1 expression and atRA levels. This work highlights the potential therapeutic benefit of restoring RBP1 expression and endogenous atRA in diseases with reduced RBP1.
Article
Substance Abuse
Bryan Mackowiak, Mingjiang Xu, Yuhong Lin, Yukun Guan, Wonhyo Seo, Ruixue Ren, Dechun Feng, Jace W. Jones, Hongbing Wang, Bin Gao
Summary: The study found that mice chronically fed with ethanol responded less severely to multiple binge ethanol consumption compared to a single binge. The upregulation of the Cyp2b10 gene after binge ethanol intake can indirectly modify ethanol metabolism and reduce acute-on-chronic ethanol-induced liver injury.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
Owen B. Becette, Anh Tran, John P. Marino, Jace W. Jones, Robert G. Brinson
Summary: In this study, a simplified and quick method for identification and assessment of a known impurity in nucleic acid drugs was developed using lithium adduct consolidation combined with mass spectrometry and nuclear magnetic resonance spectroscopy. The method is believed to have broad applicability in academic and industrial laboratories.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Genetics & Heredity
Clorissa L. Washington-Hughes, Shubhrajit Roy, Herana Kamal Seneviratne, Senthilkumar S. Karuppagounder, Yulemni Morel, Jace W. Jones, Alex Zak, Tong Xiao, Tatiana N. Boronina, Robert N. Cole, Namandje N. Bumpus, Christopher J. Chang, Ted M. Dawson, Svetlana Lutsenko
Summary: This study reveals the importance of Cu transporter Atp7b in maintaining Cu homeostasis in the brain during postnatal development. Loss of Atp7b function leads to morphological changes in choroid plexus and affects Cu transporters Atp7a and Slc31a, resulting in Cu deficit and associated imbalance of catecholamines and lipids. These findings contribute to our understanding of the neuropathology of Wilson disease.
Article
Biochemical Research Methods
Yulemni Morel, Jace W. Jones
Summary: Lipid peroxidation is crucial in the development of various diseases, as it causes oxidative damage to lipids, leading to dysfunction and death of cells. This study presents an analytical approach for characterizing the structure of oxidized products of specific types of phospholipids, providing insights into the initial impact of lipid peroxidation on cellular membranes and their role in redox biology.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Stephanie Zalesak-Kravec, Weiliang Huang, Jace W. Jones, Jianshi Yu, Jenna Alloush, Amy E. Defnet, Alexander R. Moise, Maureen A. Kane
Summary: This study investigated the impact of CRBP1 loss on the adult mouse heart using a multi-omics approach. The results showed that RA levels were decreased in Rbp1(-/-) mouse heart tissue, and metabolomics and proteomics analyses revealed disrupted pathways related to cellular metabolism and cardiac metabolism. These findings characterize the effect of CRBP1 loss and reduced RA in the adult heart.
Article
Biochemical Research Methods
Anh Tran, Liting Wan, Zhenbo Xu, Janette M. Haro, Bing Li, Jace W. Jones
Summary: The research team utilized lithium adduct consolidation to enhance mass-spectrometry-based sphingolipid analysis and implemented an optimized MALDI matrix system for high-throughput detection. They successfully applied the technology to detect differentially expressed sphingolipids in liver, brain, and plasma matrices, validating the method's efficacy.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)