Article
Multidisciplinary Sciences
Xiaolin Zhang, Qingping Lan, Mingyu Zhang, Fan Wang, Keyi Shi, Xiaojuan Li, Ersheng Kuang
Summary: Inflammasomes are important innate immune defenses against viral and bacterial infections. Various inflammasome sensors can detect invading pathogens and trigger inflammasome activation and/or pyroptosis in infected cells. Kaposi's sarcoma-associated herpesvirus (KSHV) employs a protein called SOX to suppress AIM2 inflammasome activation, promoting viral replication and inhibiting pyroptosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Bangzuo Ning, Jingjing Shen, Fanglin Liu, Hemin Zhang, Xin Jiang
Summary: Current strategies for treating drug-resistant tuberculosis are limited in effectiveness and have undesirable side effects. Therefore, research on new treatments, including innovative medications, is required. Host-directed therapy (HDT) has emerged as a viable strategy for enhancing protective immunity against infections. In this study, baicalein, derived from Scutellariae radix, was found to inhibit pyroptosis in Mycobacterium tuberculosis-infected macrophages and promote autophagy, suggesting its potential as a new adjunctive HDT drug.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Guoxing You, Linbo Zheng, Yuanyuan Zhang, Yuting Zhang, Yupeng Wang, Wenjie Guo, Hao Liu, Philipovich Tatiana, Kulchitsky Vladimir, Jie Zan
Summary: This study investigated the protective mechanism of TANG against cerebral ischemia/reperfusion (I/R) injury. The results showed that TANG could alleviate brain injury and inhibit neuronal pyroptosis induced by I/R both in vivo and in vitro. Furthermore, TANG inhibited the activation of neuronal AIM2 inflammasome via regulating NRF2. Therefore, TANG may be a potential therapeutic agent for cerebral I/R injury.
Review
Virology
Hannah L. Wallace, Rodney S. Russell
Summary: It is well-known that viruses can activate various inflammasomes, leading to programmed cell death and release of inflammatory cytokines. Traditionally, RNA viruses were thought to activate NLRP3 inflammasomes, while DNA viruses activate AIM2 inflammasomes. However, recent research suggests that viruses can activate multiple inflammasome sensors simultaneously.
Article
Immunology
Peng Xu, Wei Tong, Chen-Yen Kuo, Han-Hsiang Chen, Robert Y. L. Wang
Summary: This study investigates the potential roles of Upf1 in EV-A71-infected cells. The results suggest that Upf1 is a host protein unfavorable for EV-A71 replication. Knockdown of Upf1 protein increases viral RNA expression and production of progeny virus, while overexpression of Upf1 protein decreases viral RNA expression and production of progeny virus. Additionally, EV-A71 infection suppresses host NMD activity.
MICROBES AND INFECTION
(2023)
Article
Chemistry, Multidisciplinary
Xinyu Xu, Huanhuan Fan, Ying Yang, Shankun Yao, Wenhao Yu, Zijian Guo, Weihong Tan
Summary: By self-assembling virus-like particles with long DNA building blocks generated through rolling-circle amplification and covered with cationic liposomes, cGAS liquid phase condensation and STING signaling activation can be efficiently induced, leading to the production of inflammatory cytokines and boosting antitumor immunity. Additionally, these virus-like particles can also trigger the formation of AIM2 inflammasome and induce gasdermin D-mediated pyroptosis, further enhancing antitumor immunity. Thus, this study provides a simple and robust strategy for cancer immunotherapy for clinical application.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Medicinal
Yan Jiao, Jinshan Nan, Bo Mu, Yun Zhang, Nenghua Zhou, Shunhua Yang, Shanshan Zhang, Wanting Lin, Falu Wang, Anjie Xia, Zhixing Cao, Pei Chen, Zhiling Pan, Guifeng Lin, Shulei Pan, Huachao Bin, Linli Li, Shengyong Yang
Summary: By screening against NLRP3-dependent pyroptosis, a novel compound J114 was discovered, which displayed significant inhibitory activity against human cell pyroptosis and showed distinct differences from existing pyroptosis inhibitors. Further studies revealed that J114 inhibited inflammasome activation by disrupting the interaction between NLRP3 or AIM2 and the adaptor protein ASC.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Rajendra Karki, Balamurugan Sundaram, Bhesh Raj Sharma, SangJoon Lee, R. K. Subbarao Malireddi, Lam Nhat Nguyen, Shelbi Christgen, Min Zheng, Yaqiu Wang, Parimal Samir, Geoffrey Neale, Peter Vogel, Thirumala-Devi Kanneganti
Summary: The study uncovers the role of ADAR1 in suppressing ZBP1-mediated PANoptosis and promoting tumorigenesis. Additionally, the use of IFN-gamma and NEI KPT-330 regresses melanoma in a ZBP1-dependent manner, providing important insights for cancer therapeutic strategies.
Article
Neurosciences
Mengyao Zhao, Boya Zhang, Linlin Deng, Liming Zhao
Summary: In this study, the mechanisms of ACR-induced neuroinflammation and pyroptosis were investigated using a neuron cell model. The results showed that ACR treatment induced lytic cell death through two different pyroptotic pathways in SH-SY5Y cells. Additionally, the NLRP3 inflammasome cascade was found to be activated first.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Jing Tian, Dapeng Zhou, Liangbi Xiang, Bing Xie, Baichuan Wang, Yang Li, Xinwei Liu
Summary: Calycosin may inhibit AIM2 inflammasomes-mediated inflammation and pyroptosis through NF-kappa B and p62-Keap1 pathways, ultimately playing a protective role in gouty arthritis.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Gastroenterology & Hepatology
Kaitao Zhao, Fangteng Guo, Jingjing Wang, Youquan Zhong, Junzhu Yi, Yan Teng, Zaichao Xu, Li Zhao, Aixin Li, Zichen Wang, Xinwen Chen, Xiaoming Cheng, Yuchen Xia
Summary: We investigated the process of HBV infection in murine hepatic cells and found that HBV cannot infect murine hepatic cells expressing hNTCP effectively. Moreover, we discovered that the disassembly of cytoplasmic HBV nucleocapsids is restricted in murine hepatic cells. Overcoming this limitation may help to establish an HBV infection mouse model.
Review
Biochemistry & Molecular Biology
Lee M. Booty, Clare E. Bryant
Summary: The discovery of pyroptosis and its implications in infection and immunity has provided new insights into host defense against pathogens. However, the regulation of pyroptosis is complex and varies between cell types, stimulation conditions, pathogens, and animal species. Excessive inflammation caused by pyroptosis contributes to the development of sepsis. Understanding the regulation of pyroptotic pathways is crucial for identifying potential therapeutic targets for sepsis.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Xiaoyan Zhang, Jinfang Hao, Chenxi Sun, Jianping Du, Qian Han, Qingshan Li
Summary: Total astragalosides (ASTs) from Astragalus membranaceus have a protective effect against EV71 infection by inhibiting cell apoptosis and pyroptosis, as well as suppressing EV71 replication and release.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Oncology
Huimei Zou, Min Chen, Xiuhong Wang, Jie Yu, Xiaoying Li, Ying Xie, Jun Liu, Miao Liu, Lifen Xu, Qiong Zhang, Xiaoxue Tian, Fan Zhang, Bing Guo
Summary: Lupus nephritis (LN) is an important factor in the prognosis of systemic lupus erythematosus (SLE), as it can lead to chronic kidney disease or end-stage renal disease. This study investigated the role of C/EBP beta in LN and its impact on glomerular podocytes. The findings suggest that C/EBP beta may serve as a potential target for early diagnosis and treatment of LN.
JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaqiong Li, Wei Wang, Aixia Li, Wei Huang, Shiman Chen, Fei Han, Lingcheng Wang
Summary: The study showed that dihydroartemisinin (DHA) inhibited proliferation and induced pyroptosis in breast cancer cells by activating the AIM2/caspase-3/DFNA5 axis, providing a promising therapeutic strategy for breast cancer.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Pathology
Y. T. Hooi, K. C. Ong, S. H. Tan, D. Perera, K. T. Wong
JOURNAL OF COMPARATIVE PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Jialei Sun, Thinesshwary Yogarajah, Regina Ching Hua Lee, Parveen Kaur, Masafumi Inoue, Yong Wah Tan, Justin Jang Hann Chu
SCIENTIFIC REPORTS
(2020)
Article
Medicine, Research & Experimental
Yuan Teng Hooi, Kien Chai Ong, Soon Hao Tan, David Perera, Kum Thong Wong
LABORATORY INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Devi Nallappan, Kien Chai Ong, Uma Devi Palanisamy, Kek Heng Chua, Umah Rani Kuppusamy
Summary: This study found that a fraction of myricetin derivatives (MD) from Syzygium malaccense leaf extract has preventive and therapeutic effects on obesity, hyperglycemia, and oxidative stress induced by a high-fat diet (HFD).
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Optics
Waldo Udos, Cheong-Weng Ooi, Soon-Hao Tan, Kok-Sing Lim, Yen Jie Ee, Kien Chai Ong, Harith Ahmad
Summary: This work demonstrated a biosensor based on SPR-TFBG for detecting Enterovirus A71, improving accuracy and specificity through biofunctionalization, and reducing refractive index noise to minimize interference from unknown substances.
Article
Immunology
Michelle Hui Pheng Lee, Chee Wah Tan, Han Kang Tee, Kien Chai Ong, I-Ching Sam, Yoke Fun Chan
Summary: The study aimed to develop a live-attenuated vaccine for EV-A71 by codon deoptimization and codon-pair deoptimization. The CD X and CPD X vaccine candidates elicited good neutralizing antibodies, conferred immune protection, making them promising candidates for EV-A71.
Article
Immunology
Wei-Xin Chin, Regina Ching Hua Lee, Parveen Kaur, Tian Sheng Lew, Thinesshwary Yogarajah, Hao Yuin Kong, Zi-Yun Teo, Cyrill Kafi Salim, Rong-Rong Zhang, Xiao-Feng Li, Sylvie Alonso, Cheng-Feng Qin, Justin Jang Hann Chu
Summary: VacDZ, a chimeric dengue/Zika virus vaccine candidate, demonstrates key markers of attenuation and induces a protective immune response against Zika virus in animal models, showcasing its potential as a safe and effective vaccine against Zika virus.
Article
Biotechnology & Applied Microbiology
Hui Yeng Yeannie Yap, Boon Hong Kong, Chee Sum Alvin Yap, Kien Chai Ong, Rosnah Binti Zain, Soon Hao Tan, Zuraiza Mohamad Zaini, Szu Ting Ng, Chon Seng Tan, Shin Yee Fung
Summary: This study investigates the anti-oral cancer potential of Tiger milk mushroom sclerotia extract. The results show that the extract inhibits the growth of human oral cancer cell lines and regulates molecules associated with the tumor necrosis factor pathway. The extract induces apoptosis and cell cycle arrest, demonstrating its anti-cancer properties.
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Virology
Yoke Fun Chan, I-Ching Sam, Elena Nayan, Xiu Hui Tan, Thinesshwary Yogarajah
Summary: The seroepidemiology study of Enterovirus D68 in Kuala Lumpur, Malaysia reveals that older age groups have a higher seropositivity rate for EV-D68, especially post-September 2014. The low seropositivity rate observed in children aged 1-3 years suggests they are at risk for infection.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Clinical Neurology
Kien Chai Ong, Khong Ying Ng, Chiu Wan Ng, Soon Hao Tan, Woon Li Teo, Norain Karim, Shalini Kumar, Kum Thong Wong
Summary: Neuronal infection appears to play a more important role than vasculopathy-induced microinfarction in acute NiV encephalitis.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2022)
Article
Clinical Neurology
Shu Pin Yu, Kien Chai Ong, Soon Hao Tan, David Perera, Kum Thong Wong
Article
Multidisciplinary Sciences
Pinn Tsin Isabel Yee, Soon Hao Tan, Kien Chai Ong, Kuan Onn Tan, Kum Thong Wong, Sharifah Syed Hassan, Chit Laa Poh
SCIENTIFIC REPORTS
(2019)
Meeting Abstract
Clinical Neurology
Kum Thong Wong, Tzeh Long Fu, Kien Chai Ong, Soon Hao Tan
Meeting Abstract
Clinical Neurology
Shu Pin Yu, Kien Chai Ong, Soon Hao Tan, Tomohiro Ishikawa, Kum Thong Wong
Meeting Abstract
Clinical Neurology
Tzeh Long Fu, Kien Chai Ong, Soon Hao Tan, Kum Thong Wong