Article
Biochemical Research Methods
Pavneet Kaur, Apeksha Anand, Adil Bhat, Jaswinder Singh Maras, Neena Goyal
Summary: The study identified LdMAPK1 target proteins in Leishmania through proteomics, revealing its role in regulating diverse biological processes such as metabolism, signal transduction, replication, transcription, translation, transporters and cytoskeleton/motor proteins. This implies the potential of LdMAPK1 as a therapeutic drug target for treating visceral leishmaniasis.
JOURNAL OF PROTEOMICS
(2021)
Review
Biochemistry & Molecular Biology
Anna Lubkowska, Waldemar Pluta, Aleksandra Stronska, Alicja Lalko
Summary: Heat shock proteins (HSPs) are important molecular chaperones found in most eukaryotes and bacteria, responsible for maintaining cell homeostasis and assisting viral replication. HSP70 and HSP90 families are crucial for folding and replicating viral proteins, highlighting their potential in antiviral therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hyeryon Lee, Kyung-Hwa Baek, Trong-Nhat Phan, I. Seul Park, Sangchul Lee, Jiho Kim, Joo Hwan No
Summary: This study identified a potential drug target for visceral leishmaniasis and identified small molecule inhibitors with anti-leishmanial activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Kwang-Hoon Chun, Seung-Ju Cho, Ji-Won Lee, Ji Hae Seo, Kyu-Won Kim, Seung-Ki Lee
Summary: The study identified the interaction between PKCδ and mARD1, with PKCδ phosphorylating Ser(80) and Ser(108) of mARD1 as major phosphorylation sites. This suggests that PKCδ may transduce signals by regulating the phosphorylation of ARD1.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Zahra Alimardan, Maryam Abbasi, Farshid Hasanzadeh, Mahmud Aghaei, Ghadamali Khodarahmi, Khosrow Kashfi
Summary: Heat shock proteins (Hsps), including Hsp70, Hsp90, and small Hsps, and the transcription factor FoxM1, have important roles in carcinogenesis. Hsp70 functions in cancer initiation and protein folding, and is overexpressed in human cancers. It also interacts with cochaperones and regulates the FoxM1 signaling pathway. FoxM1 is overexpressed in most human cancers and is involved in various cancer-related processes. This review covers the structure, function, and inhibitors of Hsp70, Hsp90, and FoxM1.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Infectious Diseases
Dongmei Yuan, Hanxiao Qin, Dali Chen, Jianping Chen
Summary: The study utilized RAPD technology to identify Chinese Leishmania isolates at the genomic level and developed specific markers for the L. donovani complex. The results revealed that the unknown Leishmania species causing visceral leishmaniasis in China is a unique clade distinct from L. donovani, and genetic differentiation was observed among Chinese L. donovani. The identification of specific markers for L. donovani may facilitate the development of new diagnostic tools and further exploration of gene functions related to VL.
BMC INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Sujie Lin, Jie Yang, Weiqing Wang, Pengrong Huang, Muhammad Asad, Guang Yang
Summary: This study identified the role of PxHsc70-4 and PxHsp83 in the RNA interference pathway of Plutella xylostella. Knockdown or inhibition of PxHsc70-4 and PxHsp83 led to increased gene expression of PxmRPS18 and decreased RNAi efficiency. Overexpression of PxHsc70-4 and PxHsp83 also decreased RNAi efficiency. These findings highlight the importance of Hsc70-4 and Hsp83 in the siRNA pathway in P. xylostella.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marina Serna, Ana Gonzalez-Corpas, Sofia Cabezudo, Andres Lopez-Perrote, Gianluca Degliesposti, Eduardo Zarzuela, J. Mark Skehel, Javier Munoz, Oscar Llorca
Summary: This study reveals the role of ZNHIT2 in the regulation of R2TP chaperone during the biogenesis of PRPF8. ZNHIT2 forms a complex with R2TP and alters the conformation and nucleotide state of RUVBL1-RUVBL2, affecting its ATPase activity. PRPF8 interacts directly with R2TP and this complex can incorporate ZNHIT2 and other proteins involved in the biogenesis of PRPF8.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shiyuan Wang, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou
Summary: The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) plays a crucial role in acute kidney injury (AKI) development, through its interaction with Fis1 and phosphorylation of Fis1 at Thr(34), resulting in mitochondrial fragmentation, tubular cell death, and renal dysfunction.
Article
Biochemistry & Molecular Biology
Huafeng Xu
Summary: Recent experimental studies have shown that ATP-driven molecular chaperones can stabilize protein substrates in their native structures out of thermal equilibrium. This article proposes a unifying principle based on available evidence, which explains the conversion of chemical energy from ATP hydrolysis to the conformational free energy associated with protein folding and activation.
Article
Biochemistry & Molecular Biology
Siddhi Omkar, Tasaduq H. Wani, Bo Zheng, Megan M. Mitchem, Andrew W. Truman
Summary: This study shows that APE2 and Apn2 proteins depend on the chaperone system in yeast and mammalian cells, and suggests that inhibiting chaperones may be a potential anticancer therapy targeting APE2-mediated processes.
Article
Biochemistry & Molecular Biology
Roberta Gonnella, Andrea Arena, Roberta Zarrella, Maria Saveria Gilardini Montani, Roberta Santarelli, Mara Cirone
Summary: This study suggests that targeting HSPs could be an effective strategy to reduce cell survival in primary effusion lymphoma (PEL), as it induces strong DNA damage and impairs DDR. Inhibition of HSPs also results in de-phosphorylation of STAT3, which may downregulate HSPs as well. These findings highlight the importance of targeting HSPs in cancer therapy, as it can reduce cytokine release by PEL cells and affect anti-cancer immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Parasitology
Kanok Preativatanyou, Komson Chinwirunsirisup, Atchara Phumee, Pathamet Khositharattanakool, Sakone Sunantaraporn, Padet Siriyasatien, Jerome Depaquit
Summary: Autochthonous leishmaniasis in Thailand has become a public health concern, with an increasing number of new clinical cases. A study in southern Thailand found Asian new leishmaniasis and visceral leishmaniasis transmitted by sand flies, as well as a suspected novel trypanosoma species. These findings contribute to a better understanding of the transmission of leishmaniasis and the establishment of effective prevention and control measures.
Article
Parasitology
Juliana Hoyos, Mariana Rosales-Chilama, Cielo Leon, Camila Gonzalez, Maria Adelaida Gomez
Summary: This study used hsp70 gene sequencing to identify the sources of infection of two complex species, Leishmania (Viannia) panamensis and L. (V) guyanensis, in the Leishmania genus. The results showed that hsp70 gene sequencing is an effective method for accurately identifying different species in the Leishmania genus and for studying genetic diversity within species.
PARASITES & VECTORS
(2022)
Article
Fisheries
Kifat Jahan, Hongtao Nie, Xiwu Yan
Summary: In this study, we conducted a genome-wide identification, evolutionary relationship analysis and gene expression analysis of the HSP70, HSP90, and HSF gene families in Ruditapes philippinarum. We identified 83 RpHSP70, 6 RpHSP90, and 3 RpHSF genes in R. philippinarum. The structural characteristics, chromosomal localization, and the gene structure map were constructed to reveal the characteristics of protein structures. Furthermore, the expression profiling of transcriptome data showed the expression pattern of HSP70, HSP90 and HSF genes in Manila clam from different populations, and under high and low temperature stress. In addition, we performed protein-protein interaction network analysis between HSP70, HSP90, and HSF gene family which enabled us to recognize the regulatory relationship between the two HSP gene families and the HSF gene family. Furthermore, the predicted sub-cellular location revealed a diversified subcellular distribution of HSP70, HSP90, and HSF proteins, which may be directly or indirectly associated with functional diversification under heat stress condition.
FISH & SHELLFISH IMMUNOLOGY
(2023)