Article
Clinical Neurology
Jun-Ru Lin, Ju-Fang Cheng, Yo-Tsen Liu, Ting-Rong Hsu, Kao-Min Lin, Chien Chen, Chia-Ling Lin, Meng-Han Tsai, Jin-Wu Tsai
Summary: This study aims to investigate how DCX variants affect neuronal migration defects during brain development. Through experiments, it was found that these variants decreased the microtubule binding ability and dynamics of DCX, potentially leading to neuronal migration defects.
Article
Biochemistry & Molecular Biology
Weiming Ouyang, David M. Frucht
Summary: This study reveals that the Erk1/2 inactivation-triggered and COP1-mediated c-Jun degradation is regulated by protein phosphatases, UBE2 enzymes, and an intrinsic motif of c-Jun. The C-terminus of c-Jun protein plays a critical role in facilitating its degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maorong Wen, Yunlei Cao, Bin Wu, Taoran Xiao, Ruiyu Cao, Qian Wang, Xiwei Liu, Hongjuan Xue, Yang Yu, Jialing Lin, Chenqi Xu, Jie Xu, Bo OuYang
Summary: The cytoplasmic domain of PD-L1 (PD-L1-CD) is involved in regulating PD-L1 stability and degradation. Here the authors show that membrane binding of PD-L1-CD mediates the cellular levels of PD-L1, while metformin can disrupt the interaction between PD-L1-CD and the membrane to reduce PD-L1 levels.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Rongde Qiu, Jun Zhang, Jeremy D. Rotty, Xin Xiang
Summary: Cytoplasmic dynein is activated by the dynactin complex, cargo adapters, and LIS1, with ATP hydrolysis at AAA3 regulating its activation and localization on microtubules. Dynein activation and localization in fungal hyphae depend on the interaction of different regulators, including the Hook protein, dynactin, and LIS1. Blocking ATP hydrolysis at AAA3 can lead to alternative activation pathways for dynein, impacting its interaction with LIS1 and cargo binding.
Article
Biochemistry & Molecular Biology
Laura Marruecos, Joan Bertran, Daniel Alvarez-Villanueva, Maria Carmen Mulero, Yolanda Guillen, Luis G. Palma, Martin Floor, Anna Vert, Sara Arce-Gallego, Irene Pecharroman, Laura Batlle, Jordi Villa-Freixa, Gourisankar Ghosh, Anna Bigas, Lluis Espinosa
Summary: I kappa Bs act as cytoplasmic inhibitors of NF-kB transcription factors. Phosphorylated and SUMOylated I kappa B alpha binds to histones H2A and H4, influencing chromatin association and transcriptional regulation in skin and intestine stem cells and progenitor cells. Dynamic binding of I kappa B alpha to chromatin is necessary for intestinal cell differentiation, providing insight into the restricted nuclear distribution of p-I kappa B alpha in specific stem cell compartments.
Article
Chemistry, Multidisciplinary
Li Jiang, Xiao-bing Chen, Qian Wu, Hai-ying Zhu, Cheng-yong Du, Mei-dan Ying, Qiao-jun He, Hong Zhu, Bo Yang, Ji Cao
Summary: Experimental findings demonstrate that the C terminus of DJ-1 is crucial for its homodimerization, deglycation activity, and suppression of ferroptosis.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemical Research Methods
Cheng Cheng, Tian He, Kai Chen, Yuanxia Cai, Yaoyao Gu, Lijia Pan, Peiwen Duan, Yeming Wu, Zhixiang Wu
Summary: This study aimed to explore novel ways to indirectly target N-Myc by regulating its post-translational modifications (PTMs) and therefore protein stability. The researchers discovered that p300 can modulate the protein stability of N-Myc and is correlated with poor prognosis in NB patients.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Medicine, Research & Experimental
Xiaoqian Shang, Liang Wang, Yumei Liu, Xuemei Liu, Jie Lv, Xuan Zhou, Hao Wang, Shaxika Nazierhan, Jing Wang, Xiumin Ma
Summary: The study investigated the role of CXCR3 and its ligands in diagnosing spinal tuberculosis (ST), finding that IFN-gamma, CXCR3, and CXCL10 were significantly elevated in ST patients, serving as more reliable diagnostic markers.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell Biology
Shibo Huang, Bo Cao, Jieqiong Wang, Yiwei Zhang, Elisa Ledet, Oliver Sartor, Yuqin Xiong, Shelya X. Zeng, Hua Lu
Summary: The study identifies a novel form of p53 mutation, p53 long C-terminus (p53LC), present in various human cancers, which exhibits loss-of-function and dominant-negative effects. These mutants interact with wild-type p53, retaining it in the cytoplasm and preventing it from binding to target gene promoters, thereby inhibiting its transcriptional activity. Additionally, the mutants render p53-containing cancer cells insensitive to p53-activating agents.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Chemistry, Physical
Viktoriia A. Saveleva, Kavita Kumar, Pascal Theis, Nicole Segura Salas, Ulrike I. Kramm, Frederic Jaouen, Frederic Maillard, Pieter Glatzel
Summary: This study investigates the structural composition of Fe-N-C materials for the oxygen reduction reaction in proton-exchange membrane fuel cells, before and after catalyst layer preparation, using X-ray absorption and emission spectroscopies. The results reveal the significant impact of the preparation step on the electronic structure and local coordination of Fe, emphasizing the importance of determining the Fe-N-C catalyst structure in the prepared electrode for further studies of the structure-activity-stability correlations.
ACS APPLIED ENERGY MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Asaf Shemesh, Avi Ginsburg, Raviv Dharan, Yael Levi-Kalisman, Israel Ringel, Uri Raviv
Summary: The study investigated the self-assembly of tubulin proteins, revealing that both GTP and GDP tubulin solutions rapidly reached steady state under cold and sub-critical concentration conditions for microtubule assembly. Analysis indicated a thermodynamic model of longitudinal tubulin self-association into 1D oligomers, terminated by the formation of tubulin single rings.
ACS CHEMICAL BIOLOGY
(2021)
Article
Oncology
Guoping Dong, Fangying Fan, Yao He, Yanchun Luo, Jie Yu, Ping Liang
Summary: The study investigates the value and mechanisms of peripheral lymphocyte subsets and cytokines in predicting extrahepatic spread of hepatocellular carcinoma (HCC). Data from 380 HCC patients were analyzed, and it was found that both CD4+ and CD8+ T lymphocyte subsets were independent risk factors for extrahepatic metastasis in HCC. Serum CCL5 levels were correlated with the infiltration level of intra-tumoral CD8+ T cells and the risk of extrahepatic metastasis in HCC patients.
JOURNAL OF HEPATOCELLULAR CARCINOMA
(2023)
Article
Biochemistry & Molecular Biology
Ishtiaque Rashid, Michal Hammel, Aleksandr Sverzhinsky, Miaw-Sheue Tsai, John M. Pascal, John A. Tainer, Alan E. Tomkinson
Summary: TDP1 and LigIII alpha function as key enzymes in single-strand break repair, forming a stable complex for efficient correction of repair defects. Their direct interaction is regulated by phosphorylation and alterations in amino acid sequence, providing insights into a crucial repair pathway in nonmalignant and cancer cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Medicine, General & Internal
He-Yun Sun, Zhi-Chao Min, Lei Gao, Zi-Yi Zhang, Ting-Le Pang, Ying-Jun Gao, Hong Pan, Jun Ou-Yang
Summary: IL8RB C1208T mutation may be associated with the risk of various cancers, especially prostate adenocarcinoma.
Article
Oncology
Zizi Zhou, Wenxiang Chai, Yi Liu, Yao Liu, Huiyu Pan, Qiang Wu, Xiaoming Zhang
Summary: This study reveals the important role of Cx43 in lung cancer angiogenesis and identifies its signaling pathways. Cx43 overexpression can induce an increase in pulmonary angiogenesis by promoting cell proliferation and migration and activating related proteins.
Review
Neurosciences
Belal Shohayeb, Nicholas Rui Lim, Uda Ho, Zhiheng Xu, Mirella Dottori, Leonie Quinn, Dominic Chi Hiung Ng
MOLECULAR NEUROBIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Owen L. Woodman, Kai Yee Chin, Colleen J. Thomas, Dominic C. H. Ng, Clive N. May
CURRENT MEDICINAL CHEMISTRY
(2018)
Article
Cell Biology
Ashfaqul Hoque, Nicholas A. Williamson, S. Sadia Ameen, Giuseppe D. Ciccotosto, M. Iqbal Hossain, Jonathan S. Oakhill, Dominic C. H. Ng, Ching-Seng Ang, Heung-Chin Cheng
CELL DEATH & DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Maryam Moslehi, Dominic C. H. Ng, Marie A. Bogoyevitch
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Maryam Moslehi, Dominic C. H. Ng, Marie A. Bogoyevitch
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Belal Shohayeb, Uda Ho, Yvonne Y. Yeap, Robert G. Parton, S. Sean Millard, Zhiheng Xu, Michael Piper, Dominic C. H. Ng
HUMAN MOLECULAR GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Belal Shohayeb, Naomi Mitchell, S. Sean Millard, Leonie M. Quinn, Dominic C. H. Ng
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Cell & Tissue Engineering
Dominic C. H. Ng, Dominic K. Richards, Richard J. Mills, Uda Y. Ho, Hannah L. Perks, Callum R. Tucker, Holly K. Voges, Julia K. Pagan, James E. Hudson
Article
Cell Biology
Belal Shohayeb, Uda Y. Ho, Halah Hassan, Michael Piper, Dominic C. H. Ng
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Cell Biology
Yiqiang Zhi, Xiaokun Zhou, Jurui Yu, Ling Yuan, Hongsheng Zhang, Dominic C. H. Ng, Zhiheng Xu, Dan Xu
Summary: WDR62 acts as a scaffold protein in recruiting components of the JNK signaling pathway to regulate various human diseases. Recent studies have shown that WDR62 plays a role in neural stem cell mitosis and germ cell meiosis through JNK signaling. JNK-dependent signaling may provide a promising therapeutic strategy for related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Dominic C. H. Ng, Uda Y. Ho, Miranda D. Grounds
Summary: Primary cilia are non-motile, cell cycle-associated organelles that play important roles in cellular development and disease formation. While their role in muscle remains incompletely understood, recent studies show significant contributions in skeletal muscle, particularly in regulating cell cycle progression, differentiation, and cellular self-repair. The reciprocal interactions between cilia and the extracellular matrix in skeletal muscle, as well as the influence of cilia on cell fate in fibroblasts and myofibroblasts, have critical consequences for muscle ageing, repair, and disease response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Qiao Ding, Justin Chaplin, Matthew J. Morris, Massimo A. Hilliard, Ernst Wolvetang, Dominic C. H. Ng, Peter G. Noakes
Summary: ALS is characterized by motor neuron degeneration with frequent TDP-43 cytoplasmic inclusions. Mutant TDP-43 accumulates in stress granules under stress conditions, potentially contributing to the formation of insoluble cytoplasmic inclusions in ALS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Uda Y. Ho, Chun-Wei Allen Feng, Yvonne Y. Yeap, Amanda L. Bain, Zhe Wei, Belal Shohayeb, Melissa E. Reichelt, Hayden Homer, Kum Kum Khanna, Josephine Bowles, Dominic C. H. Ng
Summary: The loss of centriolar scaffold protein WDR62 in mouse testis leads to defects in spermatogenesis, including centriole underduplication in spermatocytes and delayed manchette removal in spermatids due to delayed Katanin p80 accumulation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Genetics & Heredity
Behnam Rashidieh, Belal Shohayeb, Amanda Louise Bain, Patrick R. J. Fortuna, Debottam Sinha, Andrew Burgess, Richard Mills, Rachael C. Adams, J. Alejandro Lopez, Peter Blumbergs, John Finnie, Murugan Kalimutho, Michael Piper, James Edward Hudson, Dominic C. H. Ng, Kum Kum Khanna
Summary: CEP55 plays a critical role in embryonic development and neural development, with its defects leading to severe clinical manifestations and perinatal lethality. It regulates neural development through the Akt pathway and downstream effector Gsk3 beta, and plays a crucial role in cilia formation.
Article
Neurosciences
Qiao Ding, Kaamini Kesavan, Kah Meng Lee, Elyse Wimberger, Thomas Robertson, Melinder Gill, Dominique Power, Jeryn Chang, Atefeh T. Fard, Jessica C. Mar, Robert D. Henderson, Susan Heggie, Pamela A. McCombe, Rosalind L. Jeffree, Michael J. Colditz, Massimo A. Hilliard, Dominic C. H. Ng, Frederik J. Steyn, William D. Phillips, Ernst J. Wolvetang, Shyuan T. Ngo, Peter G. Noakes
Summary: A defect in the n-agrin-LRP4-MuSK signaling pathway has been identified in muscle from MND patients for the first time. This signaling pathway plays a crucial role in maintaining the stability of the neuromuscular junction. These findings provide a potential therapeutic target for prolonging muscle function in MND patients.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)