Article
Materials Science, Biomaterials
Zhongguo Li, Yue Zhang, Youhong Jiang, Huiwen Li, Chunyang Chen, Weisheng Liu
Summary: This study proposes a new fluorescent probe TIFC for the rapid and specific detection of intracellular Cys, which exhibits good selectivity and sensitivity. The probe can quickly respond to Cys in vivo, demonstrating low cytotoxicity and enabling high biocompatible fluorescence imaging in osteoblasts. Therefore, TIFC has the potential for preliminary diagnosis of related diseases.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Engineering, Chemical
Huan Li, Chengcheng Wang, Lei Cai, Xiang Yu, Luling Wu, Nannan Yuan, Yiming Zhu, Nengqin Jia, Tony D. James, Chusen Huang
Summary: A ratiometric fluorescent probe (CHT-P) based on the two-isophorone fluorophore was developed for the accurate quantitative detection of HNO. The probe exhibited high selectivity and sensitivity, low cytotoxicity, and could be used for ratiometric imaging of HNO in cells. Portable test strips coated with CHT-P were used for solid-state fluorescence signal readout of HNO.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Chemistry, Analytical
Wei Shu, Jin Yu, Zhuohang Li, Xiaoqian Sun, Yunpeng Wang, Yulong Wu, Jie Qin, Yu Zhang, Haibin Xiao, Xiaoling Zhang
Summary: In this study, a novel mitochondria-targeted fluorescent probe SNRF was developed for monitoring mitochondrial pH changes. SNRF showed excellent molecular charge transfer performance and could accurately track pH variations during rapamycin-induced mitophagy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Tianruo Shen, Xia Wu, Davin Tan, Zhaochao Xu, Xiaogang Liu
Summary: This study successfully developed stable and reliable ratiometric thermometers using commercially available dyes with conformational isomers, overcoming the challenges and complexity of single-fluorophore ratiometric thermometers.
Article
Spectroscopy
Ning Li, Jiabao Zhang, Mengjun Wang, Kaishuo Wang, Jinying Liu, Huilin Sun, Xingguang Su
Summary: In this study, a ratiometric fluorescent nanoprobe was developed for urea activity determination and pH sensing. The probe consisted of a pH-sensitive indicator and a pH-insensitive reference signal. The results showed that the probe had a linear response to pH values and urea concentrations, and it demonstrated high feasibility and accuracy in real water samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Yuyang Bei, Kamile Arkin, Yuxin Zheng, Xuesong Ma, Jie Zhao, Huimin Jin, Qingkun Shang
Summary: pH-sensitive blue fluorescent carbon dots (CDs) were synthesized using Toona sinensis leaves and ethylenediamine (EDA) as raw materials, with a high fluorescence quantum yield of 17.24%. The CDs can detect urea with a limit of detection (LOD) of 6.700 mmol L-1. A ratiometric fluorescent probe (CDs@5-FAM) was constructed using CDs and 5-carboxyfluorescein (5-FAM) for more sensitive urea detection, with a low LOD of 0.014 mmol L-1. Visual intelligent detection of urea was achieved using a CDs@5-FAM probe with the help of a smartphone and RGB analysis software. The proposed method is accurate and reliable, providing a new way for rapid and visual detection of urea.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Subrata Munan, Rashmi Yadav, Niharika Pareek, Animesh Samanta
Summary: The pH levels in organelles differ from the cytoplasm and accurately mapping these pH levels is crucial for cellular homeostasis. Ratiometric fluorescent probes have become the forefront of research in targeting specific organelles and monitoring their dynamics. This review summarizes the recent advances in ratiometric pH probes and discusses the challenges in developing these probes for organelle-specific pH mapping.
Article
Chemistry, Analytical
Mariagrazia Lettieri, Simona Scarano, Laura Caponi, Andrea Bertolini, Alessandro Saba, Pasquale Palladino, Maria Minunni
Summary: We developed a simple and low-cost assay for copper in urine using a serotonin-derived fluorophore. The assay showed good reproducibility and low detection limits in buffer and artificial urine, and demonstrated excellent analytical performances in human urine samples. This is the first example of copper ion detection exploiting the fluorescence quenching of a biopolymer, offering a potential diagnostic tool for copper-dependent diseases.
Article
Chemistry, Analytical
Kun Zhang, Guowei Ding, Fengqing Gai, Yafang Zhang, Xiaoni Wang, Zhiming Gou, Yujing Zuo
Summary: Autophagy is crucial for cellular health, and its dysfunction is associated with various conditions. The development of a polysiloxane-based probe that targets lysosomes and responds to pH changes offers a valuable tool for visualizing and studying autophagic processes. This innovative probe provides insights into the mechanisms of autophagy and potential therapeutic strategies for related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Xiao-Lei Xue, Yang Wang, Shaojin Chen, Kun-Peng Wang, Shu-Yan Niu, Qian-Shou Zong, Yi Jiang, Zhi-Qiang Hu
Summary: Monitoring intracellular pH using ratiometric fluorescent probes is important for studying biological processes including diseases. This study presents a new hemicyanine-based ratio-metric fluorescent probe with a hydroxyl group, which exhibits dual emission and can selectively respond to micro-environmental pH conditions in a range of 6.0 to 8.0. The probe can enter and accumulate in mitochondria and monitor pH microenvironmental conditions through fluorescence imaging in HepG2 cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Jisoo Kang, Jong Min An, Eun Woo Seo, Jaehui Lee, Youngbuhm Huh, Chan Park, Jaehoon Kim, Dokyoung Kim
Summary: In this study, a new fluorescent probe for hydrazine detection was developed. The probe showed high stability and could detect hydrazine quickly and accurately.
Article
Chemistry, Multidisciplinary
Sarah Helen Needs, Sultan Ilayda Donmez, Alexander Daniel Edwards
Summary: The composition of human urine can significantly affect antibiotic minimum inhibitory concentration measurements when using microfluidic devices, highlighting the importance of assessing matrix interference with individual samples. Dilution and inclusion of buffer can reduce matrix interference, but may impact analytical sensitivity, especially for miniaturized devices testing small sample volumes. It is essential to fully assess and optimize novel microbiology tools using multiple individual urine samples to ensure clinical performance of rapid diagnostics to combat antimicrobial resistance.
Article
Chemistry, Analytical
Zhonglong Wang, Yan Zhang, Yurong Zhu, Yubin Fu, Xinru Zhang, Zheyu Shen, Shuai Gong, Zhiyuan Meng, Shifa Wang
Summary: A lysosome-targeted fluorescent probe Lyso-CMCP was developed for real-time monitoring pH changes in living cells. This probe showed sensitive and selective response to acidic pH and had favorable features including good photostability, reversibility, anti-interference ability, and low cytotoxicity. The lysosomal pH fluctuations were successfully monitored and quantified by ratiometric fluorescence imaging using this probe.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Xiao Liu, Xiangyang Gong, Jie Yuan, Xiaopeng Fan, Xingxing Zhang, Tianbing Ren, Sheng Yang, Ronghua Yang, Lin Yuan, Xiao-Bing Zhang
Summary: A new dual-stimulus responsive near-infrared reversible ATP-pH probe has been developed for fluorescence and photoacoustic ratiometric imaging of tumors, effectively reducing cumulative response after intravenous injection and suitable for in vivo imaging.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
