Article
Biochemical Research Methods
Shaobo Yang, Hong Yang, Yanlei Huang, Gong Chen, Chun Shen, Shan Zheng
Summary: Hirschsprung's disease (HSCR) is a congenital digestive tract malformation characterized by the absence of intramural ganglion cells in the myenteric and submucosal plexuses along variable lengths of the gastrointestinal tract. This study used metabolomic profiling to analyze HSCR serum samples and identified 21 biomarkers related to HSCR. It revealed that tryptophan metabolism was a crucial disordered pathway in HSCR. This is the first serum metabolomics study focusing on HSCR and provides a new perspective for explaining its mechanism.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Karollyny Roger Pereira Lima, Francisco Lucas Pacheco Cavalcante, Stelamaris de Oliveira Paula-Marinho, Isabelle Mary Costa Pereira, Lineker de Sousa Lopes, Joao Victor Serra Nunes, Italo Antonio Cotta Coutinho, Eneas Gomes-Filho, Humberto Henrique de Carvalho
Summary: Environmental stresses affect protein folding in the endoplasmic reticulum (ER), leading to disruptions in primary metabolism and morphophysiological changes in sorghum seedlings. The roots are more affected than the shoots, and distinct metabolomic profiles were observed.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yoji Saeki, Akane Takenouchi, Etsuyo Otani, Minji Kim, Yumi Aizawa, Yasuko Aita, Atsumi Tomita, Masahiro Sugimoto, Takashi Matsukubo
Summary: This study evaluated the effect of long-term mastication on salivary metabolomic profiles and found that most metabolites showed lower concentrations after chewing. The pathways related to glycogenic amino acids also underwent significant changes.
Article
Oncology
Alicja Pakiet, Agata Jedrzejewska, Katarzyna Duzowska, Alina Waclawska, Patrycja Jablonska, Jacek Zielinski, Adriana Mika, Tomasz Sledzinski, Ewa Slominska
Summary: The aim of this study was to examine the serum fatty acids (FAs) profiles in breast cancer survivors to assess if the FA levels normalize. After treatment for breast cancer, the patients' serum FA profile differs from the profile before treatment and from controls, especially 12 months after treatment. Some changes may reflect lifestyle changes in breast cancer survivors and have an impact on the risk of recurrence.
Article
Physiology
Lianying Guo, Jun Kuang, Yu Zhuang, Jialin Jiang, Yan Shi, Cheng Huang, Changming Zhou, Puzhi Xu, Ping Liu, Cong Wu, Guoliang Hu, Xiaoquan Guo
Summary: This study established a model of FLHS in laying hens induced by feeding a high-energy, low-protein diet, and identified 40 differential metabolites closely related to the occurrence and development of FLHS through GC-TOF-MS analysis of serum. 10 potential biomarkers with high diagnostic values were discovered, providing insights into early risk evaluation and further understanding of FLHS development.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tiago A. H. Fonseca, Cristiana P. Von Rekowski, Ruiben Araujo, M. Conceicao Oliveira, Goncalo C. Justino, Luis Bento, Ceciïlia R. C. Calado
Summary: Biofluid metabolomics is a powerful tool for studying disease mechanisms and biomarkers. This study evaluated the impact of two extraction protocols on serum metabolome analysis. The results showed that different protocols can affect the number and type of features identified, as well as the reproducibility of the analysis.
Article
Multidisciplinary Sciences
Tsz Hei Fong, Wangpan Shi, Guohui Ruan, Siyi Li, Guanghui Liu, Leyun Yang, Kaibin Wu, Jingxian Fan, Chung Lam Ng, Yafang Hu, Haishan Jiang
Summary: This study retrospectively reviewed medical records and constructed a tuberculostearic acid (TBSA)-combined scoring system based on multiple factors, which showed promising results for predicting and diagnosing tuberculous meningitis (TBM).
Article
Nutrition & Dietetics
Dadi Xie, Yanbo Zhang, Yujin Guo, Xianzhong Xue, Shiyuan Zhao, Chunmei Geng, Yuanyuan Li, Rui Yang, Yizhang Gan, Hanbing Li, Zhongfa Ren, Pei Jiang
Summary: The objective of this research was to observe and evaluate the impact of dietary components on metabolomic profiles in primary tissues of mice. The levels of metabolites in mice were studied and analyzed using gas chromatography-mass spectrometry. The results showed significant differences in metabolites related to energy, amino acids, and lipid metabolism.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Analytical
Dezhi Shan, Peng Qi, Daming Wang
Summary: Cerebrovascular stenosis (CVS) is the main cause of ischemic stroke and early screenings for CVS are important. This study aimed to elucidate the correlation between CVS and metabolomics to aid in early CVS screening. Differential metabolites were identified in patients with CVS, providing insights into the pathogenesis and early diagnosis of CVS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Zoology
Ying Xiong, Yan Hao, Yalin Cheng, Liqing Fan, Gang Song, Dongming Li, Yanhua Qu, Fumin Lei
Summary: This study investigates the pectoralis phenotypic variation in highland birds and explores its regulatory and metabolic mechanisms using phenomic, transcriptomic, and metabolomic approaches. The findings show that highland birds have increased muscle oxidative capacity, capillarity, and mitochondrial abundance. Transcriptomic analyses reveal differentially expressed genes related to blood vessels, muscle structure development, and mitochondrial organization. Importantly, all highland birds share improved insulin sensitivity and glucose utilization through activating the insulin signaling pathway, which is vital for increasing muscle oxidative capacity and maintaining metabolic homeostasis. Furthermore, there are differences in fatty acid biosynthesis and oxidation among highland bird species. The study contributes to understanding the regulatory and metabolic basis of pectoralis variation in altitudinal songbirds.
INTEGRATIVE ZOOLOGY
(2022)
Article
Biochemical Research Methods
Juan J. Aristizabal-Henao, Dominick J. Lemas, Fmily K. Griffin, Kaylie Anne Costa, Camden Camacho, John A. Bowden
Summary: The number of metabolomics studies has increased significantly in recent years, with advancements in analyte separation techniques and databases playing a major role in this growth. Establishing baseline metabolomics data using complementary data acquisition strategies can help address challenges in comparing data across studies. This study provides valuable nontargeted metabolomics data for various human reference materials, highlighting significant differences between sample types and supporting the use of nontargeted strategies in global metabolomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Isabel Meister, Pei Zhang, Anirban Sinha, C. Magnus Skold, Asa M. Wheelock, Takashi Izumi, Romanas Chaleckis, Craig E. Wheelock
Summary: This study developed a method for measuring specific gravity in urine using a refractive index detector (RID) in a 96-well-plate format, providing a new solution for metabolomic research on this noninvasive biofluid. By developing an automated LC-MS workflow and utilizing multiple technical internal standards to monitor data quality, over 540 urinary metabolites were successfully identified.
ANALYTICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Yinan Chen, Lei Hu, Hexin Lin, Huangdao Yu, Jun You
Summary: This study identified the serum metabolic signature of AEG for the first time. The distinguishing metabolites have promising applications in clinical diagnosis and outcome prediction, and help us understand the underlying metabolic mechanisms in AEG.
Article
Multidisciplinary Sciences
Mateusz Maciejewski, Caroline Sands, Nisha Nair, Stephanie Ling, Suzanne Verstappen, Kimme Hyrich, Anne Barton, Daniel Ziemek, Matthew Lewis, Darren Plant
Summary: Methotrexate is a common first-line treatment for rheumatoid arthritis, but it is ineffective for a significant portion of patients, with no reliable way to predict individual responses. Statistical models based on serum lipid levels were explored for predicting methotrexate response in patients, but clinical covariates alone outperformed lipid-based models in predicting response. Serum lipid profiles at pre-treatment or early-treatment time points are unlikely to be predictive of methotrexate response by 6 months in a way that would be useful for clinical management of rheumatoid arthritis.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Chenxi Wang, Yue Zhang, Hui Ding, Meifang Song, Jiaxin Yin, Heshui Yu, Zheng Li, Lifeng Han, Zhonglian Zhang
Summary: This study compared Zingiber corallinum and Zingiber montanum through chloroplast genome sequencing and gas chromatography-mass spectrometry analysis, finding that the chloroplast genome may not accurately identify the species. An untargeted metabolomics strategy was proposed to distinguish between the two Zingiber species using metabolic markers, and the performance of the approach was verified with unknown samples. The study suggests that the GC-MS based metabolic fingerprint is more promising for species identification and safe application of Z. corallinum and Z. montanum.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Energy & Fuels
Fahad Khan, Sana Malik, Ayesha Shahid, Amna Jabbar Siddiqui, Syed Ghulam Musharraf, Hui Zhu, Nuha Abdulhamid Alkhattabi, Munazza Gull, Muhammad Aamer Mehmood
Summary: This study evaluated the potential of a newly isolated cyanobacterium for its applications in multiproduct biorefinery. By optimizing growth conditions and using wastewater as a low-cost growth medium, the strain showed enhanced biomass production, increased protein and lipid content, and efficient biosynthesis of valuable pigments.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Pharmacology & Pharmacy
Faisal Khan, Hamad Ali, Syed Ghulam Musharraf
Summary: Sickle-cell disease and beta-thalassemia are global public health issues. This study demonstrates the effectiveness of Tenofovir disoproxil fumarate in promoting erythroid proliferation, differentiation, and HbF expression, providing a potential therapy for beta-hemoglobinopathies.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Immunology
Nurmeen Adil, Amna Jabbar Siddiqui, Syed Ghulam Musharraf
Summary: Metabolomics is a promising approach for studying metabolites in the biological system. It offers new opportunities for assessing disease progression and biomarker selection, and provides a better understanding of the pathophysiology of diseases. Autoimmune liver disease, including autoimmune hepatitis, primary biliary cholangitis, and primary sclerosing cholangitis, is a major global public health concern, with poorly understood pathogenesis. This review provides a comprehensive overview of the role of metabolomics in these diseases and discusses the disturbed metabolic pathways.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Muhammad Usman, Arslan Ali, Amna Jabbar Siddiqui, Fizza Iftikhar, Sindhia Kumari, Syed Sibt-E-Hassan, Raja Shad, Tahir Rafique, Syed Kashif Raza, Hesham R. El-Seedi, Jalal Uddin, Syed Ghulam Musharraf
Summary: Drinking water is the main source of fluoride intake for the human body. Excessive fluoride consumption can lead to fluorosis disease. This study evaluated the impact of fluoride on human serum metabolites using metabolomics, and identified significant differences in metabolites between disease and healthy samples, mainly related to energy metabolism, fatty acid oxidation, and purine degradation pathways.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Review
Medicine, Research & Experimental
Nermeen Yosri, Sultan M. Alsharif, Jianbo Xiao, Syed G. Musharraf, Chao Zhao, Aamer Saeed, Ruichang Gao, Noha S. Said, Alessandro Di Minno, Maria Daglia, Zhiming Guo, Shaden A. M. Khalifa, Hesham R. El-Seedi
Summary: Arctium lappa, also known as burdock, is a medicinal edible plant that has been used in traditional Chinese medicine for centuries. It has demonstrated various pharmacological effects and has potential applications in nanomedicine.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Engineering, Chemical
Naseer Ahmad, Sufian Rasheed, Khalid Ahmed, Syed Ghulam Musharraf, Muhammad Najam-ul-Haq, Dilshad Hussain
Summary: A two-step method is proposed to prepare multifunctional superhydrophobic coating on cotton fabric, which involves anchoring TiO2 nanoparticles on the fabric surface and treating it with myristic acid to achieve hydrophobicity. The modified fabric shows excellent stability and various desirable characteristics, making it suitable for commercial applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Tajwali Khan, Fizza Iftikhar, Rafia Akhlaq, Syed Ghulam Musharraf, Arslan Ali
Summary: Hypoxia and acidosis are common characteristics of the tumor microenvironment and affect the metabolism of cancer cells. These stresses change the post-translational modifications of histones, leading to tumorigenesis and drug resistance. However, the impact of these changes on oral squamous cell carcinoma (OSCC) is not well understood. This study investigated the effects of hypoxic and acidotic microenvironment on histone acetylation and methylation in OSCC using LC-MS-based proteomics. It identified specific histone marks associated with gene regulation and revealed the position-dependent changes in histone modifications. The findings provide insights into the adaptation of tumor cells to hypoxic and acidotic stresses and their connections with histone crosstalk.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Medicinal
Rafia Akhlaq, Tajwali Khan, Tehmina Ahmed, Syed G. G. Musharraf, Arslan Ali
Summary: Hypoxia is a characteristic feature of solid tumors, and the hypoxia-inducible factor 1-alpha (HIF-1 alpha) is a key regulator of the tumor microenvironment. Vorinostat and PX-12 are inhibitors of HIF-1 alpha with different mechanisms of action, and their combination shows synergistic effects against oral squamous cell carcinoma (OSCC) under hypoxic conditions.
DRUG DEVELOPMENT RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Ali Raza, Moatter Zehra, Muhammad Ramzan, Amna Jabbar Siddiqui, Azra Akbar, Ayaz Ahmed, Syed Ghulam Musharraf
Summary: To understand the development of antibiotic resistance in Klebsiella pneumoniae, this study conducted metabolomics analysis on sensitive and gentamicin-resistant strains. The results showed that increasing gentamicin concentration led to morphological changes in K. pneumoniae colonies, and strains grown at the highest concentration were considered resistant. Metabolomic analysis identified several regulated metabolites associated with resistance development, which could potentially be used for rapid detection of gentamicin-resistant clinical strains.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Noman Khan, Junaid Ullah, Satwat Hashmi, Arslan Ali, Amna Jabbar Siddiqui, Shahid Ahmed Sami, Syeda Saira Bokhari, Hasanat Sharif, Jalal Uddin, Hesham R. El-Seedi, Syed Ghulam Musharraf
Summary: This study aims to investigate the role of metalloproteins in the pericardial fluid of ischemic heart disease (IHD) patients. By analyzing the samples from patients with different left ventricular ejection fraction (LVEF) levels, a total of 154 metalloproteins were identified, among which the copper binding protein ceruloplasmin and the iron binding protein serotransferrin were found to be associated with the ferroptosis pathway in IHD patients.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Aphalaine Pinchemell Tiombou Donkia, Billy Toussie Tchegnitegni, Borice Tapondjou Tsafack, Roland Tchuenteu Tchuenguem, Beaudelaire Kemvoufo Ponou, Remy Bertrand Teponno, Jean Paul Dzoyem, Syed Ghulam Musharraf, Leon Azefack Tapondjou
Summary: A new 3-arylcoumarin and a previously unreported triterpenoid, along with five known flavonoids and one carbohydrate, were isolated from the roots of Desmodium salicifolium. Their structures were determined through extensive spectroscopic and mass spectrometric analyses. The methanolic extract, EtOAc and n-BuOH fractions, as well as some isolated compounds, were evaluated for their antibacterial and antioxidant activities. The EtOAc fraction exhibited moderate activity against Enterococcus faecalis, and the methanolic extract and EtOAc fraction showed DPPH scavenging activity. Compound 1 showed a moderate antibacterial activity against Enterococcus faecalis and moderate DPPH scavenging activity.
NATURAL PRODUCT RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Muhammad Ramzan, Ali Raza, Zaib Un Nisa, Syed Ghulam Musharraf
Summary: The continuous development of resistance to antibiotic drugs by microorganisms causes high mortality and morbidity. Pathogens with distinct features and biochemical abilities make them destructive to human health. Therefore, early identification of the pathogen is of substantial importance for quick ailments and healthcare outcomes. Several advanced spectroscopic techniques have been utilized for the identification of microorganisms, particularly bacteria, in research studies conducted in the last five years. Among these techniques, mass spectrometry (MS), infrared (IR) spectroscopy, Raman spectroscopy (RS), and nuclear magnetic resonance (NMR) spectroscopy have been widely used. MALDI-TOF/MS, a type of MS technique, has been particularly favored for microbial identification. The review discusses the identification of 44 bacteria using these techniques, with the most studied organisms being E. coli, S. aureus, and other microorganisms.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Adeeba Khadim, Syed Usama Yaseen Jeelani, Muhammad Noman Khan, Sindhia Kumari, Ali Raza, Arslan Ali, Bibi Zareena, Syed Muhammad Zaki Shah, Syed Ghulam Musharraf
Summary: This study developed a high-resolution mass spectral library for the rapid identification of 158 veterinary drugs in food samples. The utility of the developed database was confirmed by identifying 17 veterinary drugs in the analyzed samples. This study will assist in the high-throughput screening of multiclass veterinary drugs in foodstuffs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sabiha Farooq, Sobia Rana, Amna Jabbar Siddiqui, Ayesha Iqbal, Adil Anwar Bhatti, Syed Ghulam Musharraf
Summary: Globally, obesity is a severe health issue and a more precise approach is needed to enhance clinical care and drug development. This study investigates the association between metabolites and obesity-related anthropometric traits based on the FTO rs1421085 gene variant. The study identifies two metabolites, glycerol and 2,3-dihydroxypropyl stearate, that are significantly associated with the FTO rs1421085 gene variant and obesity-related anthropometric traits.
Review
Chemistry, Multidisciplinary
Muhammad Ikram Nabeel, Dilshad Hussain, Naseer Ahmad, Muhammad Najam-ul-Haq, Syed Ghulam Musharraf
Summary: This review focuses on the widely used graphitic carbon nitride (g-C3N4)-tungsten oxide (WO3) nanocomposite in photocatalytic applications. The article discusses the properties, synthesis methods, characterization, and photocatalytic applications of these materials. The sustainable properties, easy preparation, and high stability make them attractive for photocatalysis.
NANOSCALE ADVANCES
(2023)