Article
Immunology
Sang Bin Hong, Yong-Won Shin, Ja Bin Hong, Sang Kun Lee, Buhm Han
Summary: This study investigates the commonalities and interrelationships between B cell receptor (BCR) and T cell receptor (TCR) repertoires, finding that they have covarying clonal architecture and diversity. The pattern of correlations appears to change in immune-mediated diseases. The hierarchical clustering of B and T cell clonotypes shows distinct clusters with increased sequence similarity, shared motifs, and distinct amino acid characteristics. These findings suggest shared principles governing memory formation, recombination, and clonal expansion in B and T cells. A significant proportion of the BCR and TCR repertoire can be clustered into nonoverlapping and correlated clusters, providing a novel way of grouping clonotypes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Wesley Huisman, Lois Hageman, Didier A. T. Leboux, Alexandra Khmelevskaya, Grigory A. Efimov, Marthe C. J. Roex, Derk Amsen, J. H. Frederik Falkenburg, Inge Jedema
Summary: The contribution of public identical (PUB-I) and highly-similar (PUB-HS) T-cell receptor (TCR) sequences to virus-specific T-cell responses is found to be high, with a prevalence of up to 50% among individuals. These PUB-I and PUB-HS TCRs show structural relatedness and may be potential candidates for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Evolutionary Biology
Agusto R. Luzuriaga-Neira, Andrew M. Ritchie, Bryan L. Payne, Oliver Carrillo-Parramon, David A. Liberles, David Alvarez-Ponce
Summary: Highly abundant proteins tend to evolve slowly in a phenomenon called E-R anticorrelation. One hypothesis, the misfolding avoidance hypothesis, explains this by the toxic effects of protein misfolding. This hypothesis predicts that highly abundant proteins should exhibit high thermostability, but previous analyses have produced contradictory results. In this study, computational methods were used to compare the folding free energy of human-mouse orthologous proteins with different expression levels, finding that highly expressed proteins are often more thermostable.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Immunology
Astrid Hendriks, Malgorzata Ewa Mnich, Bruna Clemente, Ana Rita Cruz, Simona Tavarini, Fabio Bagnoli, Elisabetta Soldaini
Summary: The skin, as an immunocompetent tissue, contains various immune cells and a diverse microbiome. CD4(+) T cells play a crucial role in protecting against Staphylococcus aureus infection, and skin-specific CD4(+) T cells have been identified in response to S. aureus.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Christoph Watermann, Malin Tordis Meyer, Steffen Wagner, Claus Wittekindt, Jens Peter Klussmann, Sueleyman Erguen, Eveline Baumgart-Vogt, Srikanth Karnati
Summary: The presence and composition of peroxisomes in different cell types of the human parotid gland were investigated in this study. Through various techniques, including biochemistry and microscopy, we found that peroxisomes are present in all cells of the parotid gland, with a higher abundance in striated duct cells compared to acinar cells. The study also revealed the presence of antioxidative enzymes, such as catalase, suggesting their role in protecting against oxidative stress in the parotid gland.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shubhangini Kataruka, Veronika Kinterova, Filip Horvat, Marcos Iuri Roos Kulmann, Jiri Kanka, Petr Svoboda
Summary: miRNAs are important negative regulators of gene expression in mammalian cells, but their repressive activity is weak during oocyte and maternal-to-zygotic transition. Certain miRNAs, such as porcine ssc-miR-205 and bovine bta-miR-10b, are exceptions and can efficiently suppress gene expression despite the diluting effect of oocyte growth. The study highlights the importance of establishing a favorable miRNA:mRNA stoichiometry for effective gene suppression.
Article
Immunology
Mohamad Hamieh, Jean-Francois Chatillon, Estelle Dupel, Florence Bayeux, Emilie Fauquembergue, Pauline Maby, Aurelie Drouet, Anne-Benedicte Duval-Modeste, Sahil Adriouch, Olivier Boyer, Jean-Baptiste Latouche
Summary: Adoptive immunotherapy using artificial antigen presenting cells (AAPCs) based on mouse fibroblasts successfully expanded highly purified MART-1 specific CTLs, showing potential for efficient cancer treatment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Jiahao Yang, Lu Liu, Yasuaki Oda, Keisuke Wada, Mako Ago, Shinichiro Matsuda, Miho Hattori, Tsukimi Goto, Yuki Kawashima, Yumi Matsuzaki, Takeshi Taketani
Summary: By examining the differences between RECs and MSCs, the efficacy of mitochondrial transfer was assessed. It was found that RECs were more effective in transferring mitochondria to rho(0) cells compared to MSCs and resulted in superior recovery of mitochondrial function. Therefore, RECs might serve as a potential therapeutic strategy for diseases linked to mitochondrial dysfunction.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Multidisciplinary Sciences
Nicholas N. Jarjour, Kelsey M. Wanhainen, Changwei Peng, Noah V. Gavil, Nicholas J. Maurice, Henrique Borges da Silva, Ryan J. Martinez, Talia S. Dalzell, Matthew A. Huggins, David Masopust, Sara E. Hamilton, Stephen C. Jameson
Summary: Interleukin-15 (IL-15) is a central regulator of memory CD8+ T cells and can stimulate their proliferation and expansion. IL-15 sensitivity is an important feature of memory CD8+ T cell populations, with therapeutic potential.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, Research & Experimental
Rachel M. Gittelman, Enrico Lavezzo, Thomas M. Snyder, H. Jabran Zahid, Cara L. Carty, Rebecca Elyanow, Sudeb Dalai, Ilan Kirsch, Lance Baldo, Laura Manuto, Elisa Franchin, Claudia Del Vecchio, Monia Pacenti, Caterina Boldrin, Margherita Cattai, Francesca Saluzzo, Andrea Padoan, Mario Plebani, Fabio Simeoni, Jessica Bordini, Nicola Lore, Dejan Lazarevic, Daniela M. Cirillo, Paolo Ghia, Stefano Toppo, Jonathan M. Carlson, Harlan S. Robins, Andrea Crisanti, Giovanni Tonon
Summary: This study investigates the role of T cells in the immune response to SARS-CoV-2 infection, demonstrating their importance in both clinical presentation and long-term immunity. The findings show that T cells contribute to the production of neutralizing antibodies and remain detectable up to 15 months after infection.
Article
Immunology
Yanran Zhao, Curtis Cai, Jerome Samir, Jean-Louis Palgen, Elizabeth Keoshkerian, Hui Li, Rowena A. Bull, Fabio Luciani, Hongyan An, Andrew R. Lloyd
Summary: The study identified CD8 T-SCM subsets in healthy individuals exposed to CMV or Influenza virus, with the T(SCM)CD122(hi) expressing subset showing greater proliferation, multipotency, and polyfunctionality. The findings suggest that the T(SCM)CD122(hi) phenotype may be preferred for immune induction and adoptive immunotherapy due to its activated memory-cell like transcriptional profile.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Egor Batorov, Tatiana A. Aristova, Natalia Pronkina, Vera V. Sergeevicheva, Svetlana A. Sizikova, Galina Y. Ushakova, Ekaterina Y. Shevela, Alexander A. Ostanin, Elena R. Chernykh
Summary: Homeostatic proliferation plays apparently a key role in the upregulation of PD-1 and TIM-3 on functional T cells after AHSCT and appears to be a normal physiological process, contrary to relapse-associated increase in PD-1+ and TIM-3+ T cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Behavioral Sciences
Jing Yuan, Qin Zhang, Lixia Cui
Summary: The study found that individuals with social anxiety exhibited attentional bias under conditions of no visual working memory load, highlighting the critical role of top-down attention in social anxiety-related attentional bias.
Article
Gastroenterology & Hepatology
Young-Seon Mederacke, Mikalai Nienen, Michael Jarek, Robert Geffers, Katharina Hupa-Breier, Nina Babel, Petra Reinke, Ingmar Mederacke, Florian Wolfgang Rudolf Vondran, Danny Jonigk, Heiner Wedemeyer, Elmar Jaeckel
Summary: This study compared TCR repertoires between liver grafts and blood in liver transplant recipients with different rejection statuses. The results revealed that donor-specific T cells clonally expanded and accumulated in the transplanted liver, with distinct TCR repertoires between the liver and peripheral blood. This highlights the importance of conducting protocol liver biopsies to investigate intragraft immune responses for allo-directed immune responses.
JOURNAL OF HEPATOLOGY
(2021)
Article
Immunology
Maria Agallou, Olga S. Koutsoni, Maria Michail, Paraskevi Zisimopoulou, Ourania E. Tsitsilonis, Evdokia Karagouni
Summary: A longitudinal study conducted in 2021 found that the long-term humoral responses to SARS-CoV-2 among individuals vaccinated with the BNT162b2 vaccine were correlated with their ability to produce persistent antigen-specific T cell memory populations. mRNA vaccines elicited faster and stronger humoral responses compared to the ChAdOx1-nCoV-19 vaccine. After 7 months of vaccination, there was a decreasing trend in humoral responses. High responders had a diverse immune profile with sizable populations of memory T cells and high neutralizing antibody production, while low responders had lower antibody titers and memory T cells.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Zhirong Mou, Aida F. Barazandeh, Hiroshi Hamana, Hiroyuki Kishi, Xiaoping Zhang, Ping Jia, Nnamdi Ikeogu, Chukwunonso Onyilagha, Gaurav Gupta, Jude E. Uzonna
JOURNAL OF IMMUNOLOGY
(2020)
Article
Rheumatology
Tatsuhiko Ozawa, Kazuhisa Ouhara, Reina Tsuda, Syuichi Munenaga, Hidemi Kurihara, Hiroki Kohno, Hiroshi Hamana, Eiji Kobayashi, Hirofumi Taki, Kazuyuki Tobe, Eiji Sugiyama, Atsushi Muraguchi, Hiroyuki Kishi
ARTHRITIS & RHEUMATOLOGY
(2020)
Article
Immunology
Keiko Morita, Sayaka Tsuda, Eiji Kobayashi, Hiroshi Hamana, Kei Tsuda, Tomoko Shima, Akitoshi Nakashima, Akemi Ushijima, Hiroyuki Kishi, Shigeru Saito
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Virology
Miyuki Kimura, Kazutaka Egawa, Tatsuhiko Ozawa, Hiroyuki Kishi, Masayuki Shimojima, Satoshi Taniguchi, Shuetsu Fukushi, Hikaru Fujii, Hiroshi Yamada, Long Tan, Kaori Sano, Harutaka Katano, Tadaki Suzuki, Shigeru Morikawa, Masayuki Saijo, Hideki Tani
Summary: The heartland virus (HRTV) is a novel phlebovirus closely related to SFTSV in Asia. Research indicates that the entry mechanism of HRTV mainly involves endocytosis and Nn-DNJ may be a potential therapeutic agent against HRTV and SFTSV.
Article
Immunology
Sayaka Tsuda, Akitoshi Nakashima, Keiko Morita, Tomoko Shima, Satoshi Yoneda, Hiroyuki Kishi, Shigeru Saito
Summary: Fetal antigen-specific tolerance is crucial for maintaining allogeneic pregnancies, with a key role played by Treg cells. Insufficient or dysfunctional Treg cells may contribute to the pathogenesis of complications such as miscarriage and preeclampsia in pregnancy.
Editorial Material
Immunology
Tatsuhiko Ozawa, Eiji Kobayashi, Hiroshi Hamana, Tomoko Nakamura, Fulian Lyu, Atsushi Hayashi, Atsushi Muraguchi, Hiroyuki Kishi
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Satoshi Yamaguchi, Hiroshi Hamana, Kiyomi Shitaoka, Kenta Sukegawa, Takuya Nagata, Abdul Hayee, Eiji Kobayashi, Tatsuhiko Ozawa, Tsutomu Fujii, Atsushi Muraguchi, Kazuyuki Tobe, Hiroyuki Kishi
Summary: Tumor-infiltrating lymphocytes (TILs) are a valuable source for obtaining tumor-reactive T cell receptors (TCRs). A new evaluation system called c-FIT was developed in this study, which allowed for efficient analysis of up to 90 TCRs for their reactivity to tumor cells within a month. The system revealed interesting findings, including the identification of both HLA I-restricted and non-HLA-restricted TCRs reactive to tumor cells in CD8(+) TILs from breast cancer patients.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Oncology
Noriaki Tsuji, Kohei Hosokawa, Ryota Urushihara, Mikoto Tanabe, Takamasa Katagiri, Tatsuhiko Ozawa, Hiroyuki Takamatsu, Ken Ishiyama, Hirohito Yamazaki, Hiroyuki Kishi, Seishi Ogawa, Shinji Nakao
Summary: This study demonstrates that antigens presented by HSPCs on HLA-DR15 may lead to abnormal activation of CD4(+) T cells and immune escape in the development of AA. Immune response-related gene expression is downregulated in DR(-) cells compared to HLA-I(-) HSPCs.
Article
Engineering, Biomedical
Eiji Kobayashi, Aishun Jin, Hiroshi Hamana, Kiyomi Shitaoka, Kazuto Tajiri, Seisuke Kusano, Shigeyuki Yokoyama, Tatsuhiko Ozawa, Tsutomu Obata, Atsushi Muraguchi, Hiroyuki Kishi
Summary: By analyzing T cells at the single-cell level on a microwell array, researchers discovered that T-cell activation can occur via both trans and cis interactions, and successfully cloned tumor-antigen-specific TCRs.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Oncology
Tomoko Nakamura, Eiji Kobayashi, Hiroshi Hamana, Yoshihiro Hayakawa, Atsushi Muraguchi, Atsushi Hayashi, Tatsuhiko Ozawa, Hiroyuki Kishi
Summary: This study reports a method to generate TCR-like antibodies using a rabbit system and convert them into CAR-T cells through humanization technology. The humanized rabbit-derived CAR-T cells showed specificity and antitumor effects, potentially improving next-generation cancer immunotherapy.
Article
Multidisciplinary Sciences
Chihiro Motozono, Mako Toyoda, Toong Seng Tan, Hiroshi Hamana, Yoshihiko Goto, Yoshiki Aritsu, Yusuke Miyashita, Hiroyuki Oshiumi, Kimitoshi Nakamura, Seiji Okada, Keiko Udaka, Mizuki Kitamatsu, Hiroyuki Kishi, Takamasa Ueno
Summary: Mutations in the spike protein of SARS-CoV-2 can lead to escape from the neutralizing antibody response but still retain susceptibility to cellular immune response. A specific mutation, G446S, in the spike protein of the Omicron BA.1 variant affects antigen processing/presentation and enhances antiviral activity by vaccine-induced T cells.
NATURE COMMUNICATIONS
(2022)
Article
Rheumatology
Masatoshi Kawataka, Kazuhisa Ouhara, Eiji Kobayashi, Koichiro Shinoda, Kazuyuki Tobe, Ryousuke Fujimori, Noriyoshi Mizuno, Eiji Sugiyama, Tatsuhiko Ozawa, Hiroyuki Kishi
Summary: This study analyzed the pathogenicity of N-glycan(V) of ACPA IgG molecules in the serum of RA patients. The results showed that N-glycan(V) plays an important role in promoting osteoclast differentiation and bone resorption activity in both in vitro and in vivo assays.
Article
Cell Biology
Eiji Kobayashi, Yusuke Kamihara, Miho Arai, Akinori Wada, Shohei Kikuchi, Ryo Hatano, Noriaki Iwao, Takeshi Susukida, Tatsuhiko Ozawa, Yuichi Adachi, Hiroyuki Kishi, Nam H. Dang, Taketo Yamada, Yoshihiro Hayakawa, Chikao Morimoto, Tsutomu Sato