Article
Chemistry, Analytical
Guo Jia, Wei Li-Ping, Chen Zhi-Ying, He Cheng-Yan
Summary: The study identified 92 palmitoylated proteins in clear cell renal cell carcinoma, suggesting abnormal expression of protein acyltransferase and metabolic disorder or activation of carcinogenic signaling pathway. This improved method provides primary data for molecular targets in early diagnosis, treatment, and prognosis of clear cell renal carcinoma, contributing significantly to further research on the pathogenesis of the disease.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Valentina Buffa, Giorgia Adamo, Sabrina Picciotto, Antonella Bongiovanni, Daniele P. Romancino
Summary: Protein S-palmitoylation is a reversible lipid modification involving the addition of palmitic acid to cysteine residues. It plays various roles in cellular processes and is associated with diseases. This study presents an optimized method using Acyl Biotin Exchange (ABE) chemistry to detect protein palmitoylation. The resulting protocol is more sensitive and reproducible than the conventional approach.
Article
Chemistry, Applied
Cserne Angeli, Tamas Milan Nagy, Levente Horvath, Monika Varga, Andras Szekeres, Gabor K. Toth, Tamas Janaky, Janos Szolomajer, Melinda Kovacs, Katalin E. Kover, Tibor Bartok
Summary: This study extracted esterified fumonisin B-1 derivatives from rice cultures inoculated with Fusarium verticillioides. Three different derivatives were identified and characterized using various techniques. The experiments also revealed that F. verticillioides produces certain derivatives but not others. The identification and characterization of these derivatives are important for understanding their occurrence in different matrices and for testing their toxicity.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Polymer Science
Junlin Yuan, Conghui Li, Shanshan Wang, Hui Zhang, Zengming Wang, Aiping Zheng, Xiuli Gao
Summary: Ion-exchange resins are widely used in various fields, including pharmacy. However, completely extracting the drug from the drug-resin complex is challenging due to their specific combination. This study focused on the drug extraction from methylphenidate hydrochloride extended-release chewable tablets, using counterions for dissociation. The study found that the efficiency of drug extraction by dissociation was higher compared to other physical extraction methods, and factors affecting the dissociation process were investigated. The thermodynamic and kinetic study revealed the characteristics of the dissociation process, and the rate-limiting steps were identified. Overall, this study aims to provide support for quality assessment and control of ion-exchange resin-mediated preparations and promote their applications in drug preparation.
Article
Chemistry, Analytical
Yang Jing-Bo, He Cheng Yan, Li Ri Feng, Liu Ning
Summary: Protein palmitoylation plays important roles in the occurrence, development, and metastasis of colon cancer. In this study, an improved acyl biotinyl exchange (ABE) method was used to enrich and identify palmitoylated proteins in colorectal cancer tissues. A total of 213 highly reliable palmitoylated proteins were identified, including enolase 1, peroxide oxidoreductase, vimentin, mitochondrial malate dehydrogenase, nuclear heterogeneous ribonucleoprotein K, and chaperone complex protein 1. The results provide new insights into the molecular mechanism of palmitoylation in specific tumor-related proteins and the discovery of tumor markers or molecular targets in colon cancer.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Nuclear Science & Technology
Chun Ping Huang, Hsin Yu Chen, Wei Hsiang Lin
Summary: Two methods, fluidization with a column and immersion into a solution, were investigated for the separation of anion and cation exchange resins. These methods demonstrated effective separation, reducing the costs of radioactive waste disposal.
PROGRESS IN NUCLEAR ENERGY
(2022)
Article
Nanoscience & Nanotechnology
Chenhui Wang, Junhu Cai, Yuanyuan Ye, Xinpei Hu, Lijuan Zhong, Hongxing Xie, Enguo Chen, Yun Ye, Sheng Xu, Jie Sun, Qun Yan, Tailiang Guo
Summary: In this study, full-visible-spectrum all-inorganic perovskite quantum dots (IPQDs) were successfully synthesized using an environmentally friendly ion-exchange approach with a renewable and low-cost anion exchange resin. The introduction of the anion exchange resin improved the optical performance and surface morphology of the IPQDs. The emission wavelength of the IPQDs could be controlled without changing their crystal phase, and IPQDs with poor morphology and unstable structure could be selectively removed. The photoluminescence quantum yield and fluorescence lifetime of the IPQDs were dramatically improved. This research provides new ideas for the environmentally friendly and sustainable synthesis of IPQDs, and shows great potential in the display field.
Article
Chemistry, Analytical
Qi Ma, Yueyue Zu, Yanan Guan, Pengyi Ma, Shutao Li, Jinping Song, Yong Guo
Summary: A novel synthesis strategy of sulfur quantum dots (SQDs) using L-cysteine as a capping reagent was reported. Cys-SQDs showed high sensitivity and selectivity towards the drug molecule nimlsulide (Nim) and exhibited low biological toxicity to cells.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Kira Hallerbach, Khadijeh Khederlou, Lael Wentland, Lana Senten, Steven Brentano, Brian Keefe, Elain Fu
Summary: The use of lateral flow assays for nucleic acid detection has various applications and requires high-density capture surfaces for effective capture. This study characterized several protein-based anchors and demonstrated increased signal gains in target nucleic acid hybridization by increasing the density of capture nucleic acid. It achieved high-sensitivity nucleic acid detection in a lateral flow assay.
Article
Engineering, Chemical
Xueru Wang, Yan Chen, Wenqi Xu, Arne Lindbrathen, Xinyue Cheng, Xi Chen, Liangliang Zhu, Liyuan Deng
Summary: Direct air capture (DAC) of CO2 is a supplementary technology to achieve zero-or negative-carbon emissions. Moisture-swing adsorption, particularly using amine-based anion-exchange resins (AER), is a promising DAC technology with low costs and ease of operation. However, the limited CO2 sorption capacity and desorption efficiency of current AERs hinder the large-scale implementation of DAC. This study developed a novel diamine-based double quaternary ammonium anion-exchange resin (Diamine-DQ-AER) with significantly improved DAC capacity and stable performance.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Lidong Wang, Zhenjiang Li, Ying Liu
Summary: This study aimed to extract functional xylo-oligosaccharides (XOS) from wheat bran using ultrasonic-assisted extraction, enzymatic hydrolysis, and purification methods. The optimal conditions for extraction and hydrolysis were determined, resulting in high extraction rate and XOS content. The hydrolysate was decolorized and the purified XOS showed increased concentration.
JOURNAL OF FOOD PROCESS ENGINEERING
(2022)
Article
Biology
Sree I. Motipally, Boyden Myers, Emily R. Sechrest, David Sokolov, Joseph Murphy, Saravanan Kolandaivelu
Summary: Palmitoylation is an essential posttranslational lipid modification that plays a critical role in cellular events and the retina. It can act as a signaling PTM and its role in visual function can be better understood through the efficient isolation of retinal palmitoyl proteome. The modified acyl resin-assisted capture method offers a cost-effective and easy way to purify palmitoylated proteins from the retina for further analysis by LC-MS/MS.
Article
Engineering, Chemical
Xinlei Pan, Xiang Zou, Junguo He, Heliang Pang, Pengfei Zhang, Yijie Zhong, Jie Ding
Summary: Using cation exchange resin as a novel extracellular polymer substance disruptor can enhance lysozyme-induced enzymolysis and solubilization of dissolved organic matters, resulting in increased production of short-chain fatty acids (SCFAs) from waste activated sludge. This coupled pretreatment showed higher SCFAs yield compared to sole lysozyme pretreatment.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
R. Sharifian, R. M. Wagterveld, I. A. Digdaya, C. Xiang, D. A. Vermaas
Summary: Electrochemical CO2 capture technologies are gaining attention for their flexibility and potential to address decentralized emissions. These methods rely on pH-swing to capture and recover CO2, and are being analyzed for their energy efficiency and adaptability in a circular carbon economy. Although costly, they could become more viable with widespread availability of affordable renewable electricity and materials.
ENERGY & ENVIRONMENTAL SCIENCE
(2021)
Article
Chemistry, Physical
Sang-Woo Han, Ye-Ri Choi, Jong-Shik Shin
Summary: A novel biocatalytic process using AADC was developed for the preparative-scale production of biogenic amines, with broad substrate specificity and high conversion rates achieved under an ISPR strategy. The implementation of an open reactor system and cation exchange resin enabled efficient removal of reaction products, leading to high conversions within short reaction times.
Article
Biochemical Research Methods
Kathryn A. McGurk, Arianna Dagliati, Davide Chiasserini, Dave Lee, Darren Plant, Ivona Baricevic-Jones, Janet Kelsall, Rachael Eineman, Rachel Reed, Bethany Geary, Richard Unwin, Anna Nicolaou, Bernard D. Keavney, Anne Barton, Anthony D. Whetton, Nophar Geifman
Article
Clinical Neurology
Bangfu Zhu, Jennifer C. H. Mak, Andrew P. Morris, Anthony G. Marson, Jeff W. Barclay, Graeme J. Sills, Alan Morgan
Article
Multidisciplinary Sciences
Bernat Morro, Mary K. Doherty, Pablo Balseiro, Sigurd O. Handeland, Simon MacKenzie, Harald Sveier, Amaya Albalat
Article
Multidisciplinary Sciences
Vincenzo Alessandro Laudicella, Christine Beveridge, Stefano Carboni, Sofia C. Franco, Mary K. Doherty, Nina Long, Elaine Mitchell, Michele S. Stanley, Phillip D. Whitfield, Adam D. Hughes
Article
Behavioral Sciences
Andrew Shaw, Luke D. Arnold, Lucia Privitera, Phillip D. Whitfield, Mary K. Doherty, Lorenzo More
BEHAVIOURAL BRAIN RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Sarah F. Martin, Mary K. Doherty, Eliane Salvo-Chirnside, Seshu R. Tammireddy, Jiaxiuyu Liu, Thierry Le Bihan, Phillip D. Whitfield
Article
Cell & Tissue Engineering
Edvinas Cerniauskas, Marzena Kurzawa-Akanbi, Long Xie, Dean Hallam, Marina Moya-Molina, Kathryn White, David Steer, Mary Doherty, Phil Whitfield, Jumana Al-Aama, Lyle Armstrong, David Kavanagh, John D. Lambris, Viktor Korolchuk, Claire Harris, Majlinda Lako
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Oncology
Kelechi Njoku, Amy E. Campbell, Bethany Geary, Michelle L. MacKintosh, Abigail E. Derbyshire, Sarah J. Kitson, Vanitha N. Sivalingam, Andrew Pierce, Anthony D. Whetton, Emma J. Crosbie
Summary: Endometrial cancer is the most common cancer of the female genital tract, and this study shows that plasma-based metabolites have the potential to detect the cancer early in women with a BMI >= 30 kg/m(2). Specific lipid metabolites, including phospholipids and sphingolipids, demonstrate good accuracy for detection, especially when combined in a diagnostic model.
Article
Multidisciplinary Sciences
Angelica Arioli, Arianna Dagliati, Bethany Geary, Niels Peek, Philip A. Kalra, Anthony D. Whetton, Nophar Geifman
Summary: OptiMissP is a dashboard designed to visually and qualitatively evaluate missing values in proteomic data and guide decision-making in handling missing values. It provides visual tools and exploration of different imputation methods to help researchers and clinicians determine study-specific strategies for addressing missing values. OptiMissP also considers biases in protein distributions related to imputation methods and helps balance information loss and noise introduced by different missingness thresholds.
Article
Oncology
Bethany Geary, Erin Peat, Sarah Dransfield, Natalie Cook, Fiona Thistlethwaite, Donna Graham, Louise Carter, Andrew Hughes, Matthew G. Krebs, Anthony D. Whetton
Summary: TARGET (tumour characterisation to guide experimental targeted therapy) aims to match cancer patients entering early phase clinical trials to the best treatment based on their genetics, reducing the need for recruiting more patients. The study developed a Wellness Score using blood samples to predict patient survival more accurately than traditional performance status measurements. This score showed promising results in predicting overall survival and warrants further evaluation in larger patient populations.
Article
Medicine, General & Internal
Zohaib Iqbal, Helene A. Fachim, J. Martin Gibson, Ivona Baricevic-Jones, Amy E. Campbell, Bethany Geary, Rachelle P. Donn, Dashne Hamarashid, Akheel Syed, Anthony D. Whetton, Handrean Soran, Adrian H. Heald
Summary: Bariatric surgery induces metabolic pathway recalibration, potentially contributing to the remission of type 2 diabetes mellitus. Analysis of plasma samples identified several proteins that may serve as biomarkers for T2DM remission after surgery.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Kelechi Njoku, Davide Chiasserini, Bethany Geary, Andrew Pierce, Eleanor R. Jones, Anthony D. Whetton, Emma J. Crosbie
Summary: Endometrial cancer is the most common gynaecological malignancy in high-income countries with a rising incidence. Early detection with sensitive and specific biomarkers can improve outcomes, and SWATH-MS offers a technological advancement for biomarker discovery due to its reproducibility, sensitivity, and potential for data re-interrogation. This study presents a unique spectral library for identifying protein biomarkers in cervico-vaginal fluid for endometrial cancer detection, available via ProteomeXchange.
Article
Biochemistry & Molecular Biology
Richard Campion, Leanne Bloxam, Kimberley Burrow, Philip J. Brownridge, Daniel R. Pentland, Patricia Thomas, Campbell W. Gourlay, Claire E. Eyers, Jeff W. Barclay, Alan Morgan
Summary: Dietary restriction (DR) can increase replicative lifespan in yeast by enhancing mitochondrial respiration and affecting the expression of certain proteins, while also inhibiting mating in yeast. These changes may be related to proteins associated with the heat shock protein (HSP) family.
BIOCHEMICAL JOURNAL
(2021)
Article
Cell Biology
Helene A. Fachim, Zohaib Iqbal, J. Martin Gibson, Ivona Baricevic-Jones, Amy E. Campbell, Bethany Geary, Akheel A. Syed, Antony Whetton, Handrean Soran, Rachelle P. Donn, Adrian H. Heald
Summary: After metabolic bariatric surgery, changes in inflammatory markers and circulating proteins were observed, with some proteins correlating with alterations in IL6, TNF alpha, and CRP. Further studies are required to elucidate the mechanisms by which MBS reduces low-grade inflammation.
Article
Biochemistry & Molecular Biology
Eleanor Barker, Alan Morgan, Jeff W. Barclay
Summary: This study successfully established a Caenorhabditis elegans model for autosomal dominant adult-onset neuronal ceroid lipofuscinosis (ANCL), a rare neurodegenerative disorder. The disease-causing mutations were introduced into the worm's genome using CRISPR/Cas9 gene editing, accurately mimicking the human genetic disorder. The model exhibited similar phenotypes to ANCL patients and the impaired chemotaxis and reduced lifespan could be rescued by the anti-epileptic drug ethosuximide, suggesting its potential therapeutic use for ANCL.
HUMAN MOLECULAR GENETICS
(2023)