Article
Biochemistry & Molecular Biology
Stephanie Maia Acuna, Jonathan Miguel Zanatta, Camilla de Almeida Bento, Lucile Maria Floeter-Winter, Sandra Marcia Muxel
Summary: This study identified miR-294 and miR-410 as biomarkers for regulating the inflammatory profiles of macrophages during L. amazonensis infection. The inhibition of miR-294 and miR-410 showed potential therapeutic strategies for modulating inflammation and reducing parasitism.
Article
Microbiology
Yun-Fu Chen, Si-Fei Yu, Chang-You Wu, Na Wu, Jia Shen, Juan Shen, Jiang-Mei Gao, Yan-Zi Wen, Geoff Hide, De-Hua Lai, Zhao-Rong Lun
Summary: Leishmania infection in humans can lead to varied clinical symptoms, with outcomes influenced by both host and parasite factors. While inbred mouse strains show resistance to Leishmania major but susceptibility to Leishmania amazonensis, rats are highly resistant to L. amazonensis. The Nos2 gene appears to play a crucial role in mediating this resistance, highlighting the importance of distinct macrophage properties in innate immunity to Leishmania infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gustavo Silva-Oliveira, Leandra Linhares-Lacerda, Thayana R. F. Mattos, Camila Sanches, Tatiana Coelho-Sampaio, Ingo Riederer, Elvira M. Saraiva
Summary: Neutrophils release neutrophil extracellular traps (NETs) when they interact with different laminin (LM) isoforms. The induction of NETs by laminins is partially inhibited by anti-alpha 6 integrin antibody and requires elastase and peptidylarginine deiminase (PAD)-4. Laminins also enhance the release of NETs triggered by a protozoan parasite and exhibit leishmanicidal activity.
Article
Cell Biology
Patricia da Silva Marques, Alessandra M. da Fonseca-Martins, Monique Pacheco Duarte Carneiro, Natalia R. T. Amorim, Camila R. Rodrigues de Pao, Claudio Canetti, Bruno L. Diaz, Herbert L. de Matos Guedes, Christianne Bandeira-Melo
Summary: The study found that eosinophils regulate intracellular L. amazonensis infection in macrophages through eosinophil-derived PGD2, preventing an increase in amastigote numbers. This may serve as a complementary therapeutic tool for managing cutaneous leishmaniasis caused by L. amazonensis.
CELLULAR IMMUNOLOGY
(2021)
Article
Immunology
Olivier Seguin, Linh Thuy Mai, Hamlet Acevedo Ospina, Marie-Michele Guay-Vincent, Sidney W. Whiteheart, Simona Stager, Albert Descoteaux
Summary: This study investigates the contribution of host cell soluble N-ethylmaleimide-sensitive-factor attachment protein receptor proteins to the expansion and functionality of communal vacuoles as well as the replication of Leishmania parasites. The results show that VAMP8 contributes to the optimal expansion of communal vacuoles, while VAMP3 negatively regulates L. amazonensis replication, vacuole size, and antigen cross-presentation.
INFECTION AND IMMUNITY
(2022)
Article
Chemistry, Medicinal
Joao Victor Silva-Silva, Carla J. Moragas-Tellis, Maria S. S. Chagas, Paulo Victor R. Souza, Davyson L. Moreira, Daiana J. Hardoim, Noemi N. Taniwaki, Vanessa F. A. Costa, Alvaro L. Bertho, Daniela Brondani, Eduardo Zapp, Aldo Sena de Oliveira, Katia S. Calabrese, Maria D. Behrens, Fernando Almeida-Souza
Summary: Carajurin, the main constituent of Arrabidaea chica, shows anti-Leishmania activity by affecting mitochondrial function and inducing reactive oxygen species production. The drug-likeness assessment of Carajurin also meets certain criteria of drug chemistry.
Article
Parasitology
Mauricio S. Pena, Fenny Hui Fen Tang, Fernando Alves de Lima Franco, Alessandro Taunay Rodrigues, Guilherme Moreira Paiva Carrara, Thais Larissa Silva Araujo, Ricardo Jose Giordano, Giuseppe Palmisano, Maristela Martins de Camargo, Silvia Reni Bortolin Uliana, Beatriz Simonsen Stolf
Summary: Leishmaniases, caused by protozoan parasites, affect 12 million people worldwide. In this study, the LRR17 gene and protein were identified in Leishmania species. The protein LaLRR17 was found to increase infectivity when overexpressed in Leishmania (L.) amazonensis promastigotes. Further investigation revealed that macrophage GRP78 is a ligand for LaLRR17 and blocking this interaction prevents the increase in infection conferred by LaLRR17. These findings provide insights into the mechanism of phagocytosis in Leishmania infections.
Article
Immunology
Taylon Felipe Silva, Manoela Daiele Goncalves, Virginia Marcia Concato, Bruna Taciane da Silva Bortoleti, Fernanda Tomiotto-Pellissier, Raquel Arruda Sanfelice, Ana Carolina Jacob Rodrigues, Mariana Barbosa Detoni, Andrea Name Colado Simao, Luiz Antonio Custodio, Tania Longo Mazzuco, Idessania Nazareth da Costa, Milena Menegazzo Miranda-Sapla, Wander Rogerio Pavanelli, Ivete Conchon-Costa
Summary: The study found that peripheral blood mononuclear cells (PBMC) from diabetic patients are more susceptible to L. amazonensis infection and fail to control the infection over time due to an inability to generate effector microbicidal molecules, which results in oxidative stress and dysregulated inflammatory response.
IMMUNOLOGY LETTERS
(2021)
Article
Cell Biology
Heba Nafea, Rana A. Youness, Khaled Abou-Aisha, Mohamed Z. Gad
Summary: This study identified miR-939-5p as a tumor suppressor miRNA and HEIH as an oncogenic lncRNA in TNBC, showing their regulatory roles in the NO machinery system. Knocking down HEIH improved the immunogenic profile of TNBC cells by inducing MICA/B and suppressing PDL1, and also alleviated the immune-suppressive tumor microenvironment by repressing interleukin-10 and tumor necrosis factor-alpha levels.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Immunology
Leilane Oliveira Goncalves, Andres F. Vallejo Pulido, Fernando Augusto Siqueira Mathias, Alexandre Estevao Silverio Enes, Maria Gabriela Reis Carvalho, Daniela de Melo Resende, Marta E. Polak, Jeronimo C. Ruiz
Summary: In this study, the role of inducing factors of the Th17 pathway in Leishmania-macrophage infection was investigated through computational modeling. The results showed an increase in Th17 induction in the early stages of infection, followed by a decrease in the later stages. The generated gene regulatory network model provided an integrative and dynamic view of the Leishmania-macrophage interaction.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Thais Tenorio Soares Fujii, Pollyanna Stephanie Gomes, Rubens Lima do Monte-Neto, Daniel Claudio de Oliveira Gomes, Marc Ouellette, Eduardo Caio Torres-Santos, Valter Viana Andrade-Neto, Herbert Leonel de Matos Guedes
Summary: The study investigates the compensatory mechanisms of the sterol biosynthesis pathway in Leishmania parasites, revealing the important role of programmed regulation and gene expression in drug resistance. These findings have implications for the development of drugs and protease inhibitors targeting parasites.
Article
Medicine, Research & Experimental
Ahmed Rakib, Mousumi Mandal, Anaum Showkat, Sonia Kiran, Soumi Mazumdar, Bhupesh Singla, Aman Bajwa, Santosh Kumar, Frank Park, Udai P. Singh
Summary: The polyphenolic compound piceatannol (PC) has been found to have beneficial effects on metabolic diseases, antiproliferative, antioxidant, and anti-cancer properties. This study investigated the effects of PC on inflammatory responses and adipogenesis. The results showed that PC induced apoptosis in activated T cells, reduced immune cell activation and inflammatory response, and hindered adipogenesis.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Applied
Fernandes Nilma de Souza, Vania Cristina Desoti, Alcilene Dias, Yasmin Cunha da Silva, Ana Paula de Azevedo dos Santos, Guilherme Matos Passarini, Celso Vataru Nakamura, Valdir Florencio da Veiga Junior
Summary: This study found that the isolated compound SP from the Amazonian tree species has activity against Leishmania amazonensis, causing morphological and ultrastructural changes in parasites, cell cycle impairment, increased production of reactive oxygen species, accumulation of lipid bodies, and formation of autophagic vacuoles. In silico studies showed a high drug-score for SP, suggesting its potential for further investigation as a promising drug candidate for chemotherapy against parasitic infections.
NATURAL PRODUCT RESEARCH
(2021)
Article
Microbiology
Manuel Soto, Laura Ramirez, Jose Carlos Solana, Emma C. L. Cook, Elena Hernandez-Garcia, Jose Maria Requena, Salvador Iborra
Summary: The genetically-modified attenuated L. infantum line, Li Delta HSP70-II, was shown to prevent cutaneous leishmaniasis in BALB/c mice challenged with virulent L. amazonensis parasites. Vaccinated mice exhibited decreased disease evolution, reduced lesions, and parasite burdens, along with induction of anti-Leishmania antibodies and specific IFN-gamma responses. This live vaccine may have potential as a candidate for human vaccine development against different forms of leishmaniasis.
Article
Immunology
Yen-Ju Lin, Annette Jamin, Sonja Wolfheimer, Anna Fiedler, Ann-Christine Junker, Alexandra Goretzki, Stephan Scheurer, Stefan Schuelke
Summary: The fusion protein rFlaA:Betv1 can activate macrophages and induce higher IL-1 beta secretion. This activation involves NLRC4- and NLRP3-inflammsomes, as well as NF kappa B- and SAP/JNK MAP kinase signaling. Understanding the mechanisms of immune cell activation by rFlaA:Betv1 can help improve treatment strategies using flagellin as an adjuvant.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
G. S. Kinker, L. H. Ostrowski, P. A. C. Ribeiro, R. Chanoch, S. M. Muxel, I. Tirosh, G. Spadoni, S. Rivara, V. R. Martins, T. G. Santos, R. P. Markus, P. A. C. M. Fernandes
Summary: Melatonin has been found to have an anticancer effect on brain tumors with a negative correlation between its synthesis in the microenvironment and tumor malignancy. MT1 activation inhibits the proliferation of glioma and medulloblastoma cell lines, while MT2 activation promotes it. Tumors with a higher MT1/MT2 expression ratio show better survival rates.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Fisheries
Vinicius Queiroz, Sandra M. Muxel, Luigi Inguglia, Marco Chiaramonte, Marcio R. Custodio
Summary: The study utilized Imaging Flow Cytometry to characterize coelomocytes of sea urchins, isolate different cell populations, and observe physiological responses during a bacterial immune challenge. The results revealed distinct roles of various cell populations in the immune response, shedding light on how echinoids modulate their physiological responses.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maricruz Mamani-Huanca, Sandra Marcia Muxel, Stephanie Maia Acuna, Lucile Maria Floeter-Winter, Coral Barbas, Angeles Lopez-Gonzalvez
Summary: The research analyzed the metabolic networks of macrophages infected by Leishmania parasites and found that the absence of parasite arginase affects host and parasite metabolism pathways, increases nitric oxide (NO) production, and enhances infectivity rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Eduardo Milton Ramos-Sanchez, Luiza Campos Reis, Marina de Assis Souza, Sandra Marcia Muxel, Kamila Reis Santos, Dimitris Lagos, Valeria Rego Alves Pereira, Maria Edileuza Felinto de Brito, Paul Martin Kaye, Lucile Maria Floeter-Winter, Hiro Goto
Summary: This study investigated the changes in miRNA associated with leishmaniasis and identified different target pathways in immune and inflammatory pathways. miR-548d-3p may play a role in suppressing parasite growth, but further research is needed to understand its in vivo effects and its potential as a biomarker for progressive leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Cell Biology
Giovana Parreira de Aquino, Marco Antonio Mendes Gomes, Roberto Kopke Salinas, Maria Fernanda Laranjeira-Silva
Summary: Trypanosomiases and leishmaniases are neglected tropical diseases that have been spreading to new areas, with limited treatment options and high toxicity. Parasites rely on lipids and fatty acids for survival, providing potential targets for novel antiparasitic drug development.
Article
Biochemistry & Molecular Biology
Stefanny C. M. Titon, Braz Titon Jr, Sandra M. Muxel, Aymam C. de Figueiredo, Felipe R. Floreste, Alan S. Lima, Fernando R. Gomes, Vania R. Assis
Summary: The immune response in R. icterica toads following LPS injection was characterized by increased PP, NLR, and IL-1 beta mRNA expression, along with activation of the hypothalamic-pituitary-interrenal axis. The timing of the injection affected the endocrine and immune mediators, with higher CORT levels and lower inflammatory response observed during the night.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Zoology
Felipe R. Floreste, Braz Titon Jr, Stefanny C. M. Titon, Sandra M. Muxel, Fernando R. Gomes, Vania R. Assis
Summary: The study investigates the hormone levels and cytokine expression in amphibians during the inflammatory response. The results show that corticosterone and melatonin levels are affected by LPS in the early stages of immune response. IL-1 beta and IL-6 are up-regulated after 1 hour, while IL-1 beta decreases after 3 hours and IL-6 remains up-regulated throughout. IL-10 is detected after 6 hours of LPS stimulation, accompanied by an increase in corticosterone levels. After 18 hours, corticosterone levels remain high, IL-1 beta is up-regulated again, and IL-6 is up-regulated with a decrease in IL-10. Positive correlations are observed between IL-1 beta and IL-6 in the LPS group.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patricio G. Garcia Neto, Stefanny C. M. Titon, Vania R. Assis, Sandra M. Muxel, Braz Titon Jr, Leticia F. Ferreira, Regina P. Markus, Fernando R. Gomes, Pedro A. C. M. Fernandes
Summary: Glucocorticoids and melatonin have immunomodulatory effects in both stimulating and suppressing the immune system, depending on the context. This study investigated the immune response of male toads in their natural habitat after lipopolysaccharide (LPS) injection. The results showed that LPS injection increased inflammation and corticosterone levels, similar to what was observed in toads in captivity. Additionally, a positive correlation between IL-1 beta and IL-6 was found, and corticosterone was negatively associated with testosterone.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carolina M. Polonio, Patrick da Silva, Fabiele B. Russo, Brendo R. N. Hyppolito, Nagela G. Zanluqui, Cecilia Benazzato, Patricia C. B. Beltrao-Braga, Sandra M. Muxel, Jean Pierre S. Peron
Summary: Viral infections, particularly Zika virus, have a significant impact on public health, causing severe fetal neuropathogenesis and microcephaly. MiRNAs play a role in infected neuronal precursor cells and directly contribute to the pathogenesis of fetal microcephaly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Kassiano S. Sousa, Caroline L. Quiles, Sandra M. Muxel, Isabela L. Trevisan, Zulma S. Ferreira, Regina P. Markus
Summary: The pineal gland plays a key role in surveillance and defense responses. Melatonin secreted by the pineal gland regulates the rolling and adhesion of leukocytes. Pathogens and pro-inflammatory cytokines inhibit nocturnal melatonin synthesis, leading to leukocyte migration to injured areas. ATP acts as a cotransmitter and enhances melatonin synthesis. The pineal gland responds differently to different doses of ATP and shows structure-dependent effects in the brain.
Article
Biochemistry & Molecular Biology
Felipe R. Floreste, Braz Titon Jr, Stefanny C. M. Titon, Sandra M. Muxel, Aymam C. de Figueiredo, Fernando R. Gomes, Vania R. Assis
Summary: This study highlights the crucial role of the liver in the inflammatory response in amphibians. The expression of immune proteins in the liver was up-regulated in the early phase, while the spleen exhibited a slower but more consistent response.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jonathan Miguel Zanatta, Stephanie Maia Acuna, Yan de Souza Angelo, Camilla de Almeida Bento, Jean Pierre Schatzman Peron, Beatriz Simonsen Stolf, Sandra Marcia Muxel
Summary: This study investigated the effects of L-arginine and polyamines on L. amazonensis infection and the transcriptional regulation of genes related to arginine metabolism and proinflammatory response in BALB/c macrophages. The results showed that putrescine supplementation reduced the percentage of infected macrophages compared to other supplements. Additionally, the availability of polyamines was found to interfere with cytokine/chemokine production.
Article
Biotechnology & Applied Microbiology
Wanessa Moreira Goes, Carlos Rodolpho Ferreira Brasil, Joao Luis Reis-Cunha, Anderson Coqueiro-dos-Santos, Viviane Grazielle-Silva, Julia de Souza Reis, Tatiane Cristina Souto, Maria Fernanda Laranjeira-Silva, Daniella Castanheira Bartholomeu, Ana Paula Fernandes, Santuza Maria Ribeiro Teixeira
Summary: We sequenced and assembled the PH8 strain of Leishmania amazonensis, an etiological agent of leishmaniasis, and obtained the sequence of 34 chromosomes with 8317 annotated genes. We identified multigene families encoding virulence factors, as well as genes involved in parasite iron and heme metabolism. We also tested CRISPR-Cas9 protocols to generate knockout cell lines for further study of virulence factors.
Article
Microbiology
Romario Lopes Boy, Ahyun Hong, Juliana Ide Aoki, Lucile Maria Floeter-Winter, Maria Fernanda Laranjeira-Silva
Summary: Protozoan parasites of the genus Leishmania are a major cause of leishmaniases, and the lack of effective vaccines and specific treatment options highlights the importance of studying the parasite's genome and proteins. Reporter gene systems can aid in understanding gene expression and function in the parasite, facilitating the discovery of new therapeutic targets for leishmaniases.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Maia Acuna, Jonathan Miguel Zanatta, Camilla de Almeida Bento, Lucile Maria Floeter-Winter, Sandra Marcia Muxel
Summary: This study identified miR-294 and miR-410 as biomarkers for regulating the inflammatory profiles of macrophages during L. amazonensis infection. The inhibition of miR-294 and miR-410 showed potential therapeutic strategies for modulating inflammation and reducing parasitism.