Letter
Gastroenterology & Hepatology
Sohyun Lee, Nanjoo Park, Sujung Yun, Eunseon Hur, Jiwon Song, Hanna Lee, Yongsug Kim, Sangryeol Ryu
Summary: The study identified the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea, with fluoroquinolone resistance mainly attributed to Salmonella 4,[5],12:i:- and Salmonella Typhimurium carrying the qnr gene.
Article
Infectious Diseases
Yan-Yan Zhou, Li-Yan Ma, Li Yu, Xin Lu, Wei-Li Liang, Biao Kan, Jian-Rong Su
Summary: In this study, the prevalence of quinolone resistance related genes among V. cholerae O139 strains isolated in China was investigated. It was found that eight strains carried the qnrVC gene, which encodes a pentapeptide repeat protein belonging to the Qnr subfamily. However, strains carrying certain qnrVC alleles showed sensitivity to ciprofloxacin, while the strain carrying qnrVC9 exhibited high resistance. It was also discovered that the qnrVC9 gene was carried on a small plasmid which could be horizontally transferred among Vibrio species.
Article
Public, Environmental & Occupational Health
Nambram Somendro Singh, Neelja Singhal, Manish Kumar, Jugsharan Singh Virdi
Summary: This study investigates the presence of antimicrobial resistance genes in Escherichia coli isolated from a major river in northern India. The results show that most strains are susceptible to fluoroquinolones and some aminoglycosides, but a significant portion exhibit resistance to streptomycin, kanamycin, and gentamicin. Plasmid-mediated quinolone resistance (PMQR) genes and aminoglycoside resistance genes are identified, implying their potential transferability through plasmids.
EPIDEMIOLOGY AND INFECTION
(2022)
Review
Infectious Diseases
Miaoling Meng, Yaying Li, Huaiying Yao
Summary: Due to the widespread use of antibiotics, antibiotic resistant genes (ARGs) are present in humans, plants, animals, and natural environments. ARGs exhibit migration and transmission in various environmental media, which can be even more harmful than antibiotics themselves. The transfer of ARGs mainly occurs among microorganisms through mobile genetic elements (MGEs) such as plasmids and phages. Soil, as the most microbially active environment, is closely associated with human activities and have become contaminated with antibiotics and ARGs. In this review, the plasmid-mediated migration and transmission of ARGs in natural environments and under different antibiotic selection pressures are discussed. The current methods of plasmid extraction and analysis are summarized, and the mechanism of plasmid conjugation using the F factor as an example is briefly introduced. However, further investigations are needed to understand the contribution of soil plasmids in ARG transmission, considering the global spread of drug-resistant bacteria and the advancements in MGE knowledge.
Article
Microbiology
Guoye Liu, Huimin Qian, Jingwen Lv, Benshun Tian, Changjun Bao, Hong Yan, Bing Gu
Summary: The study revealed an increasing trend of antimicrobial resistance in nontyphoidal Salmonella in Jiangsu, China, particularly towards colistin. Plasmid-mediated resistance genes play a significant role in these bacteria, with mcr-1-positive strains predominantly found in children under 7 years old.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Toshiyuki Murase, Patchara Phuektes, Hiroichi Ozaki, Sunpetch Angkititrakul
Summary: Plasmid-mediated quinolone resistance (PMQR) was found to be prevalent in Escherichia coli in chicken farms, as detected in chicken feces, carcass surface samples, and retail chicken meat samples. The isolates showed varying levels of resistance to different antimicrobials. It was observed that the qnrS gene is transferred horizontally through plasmids and can be co-selected with the use of TMP.
Article
Microbiology
Abimbola Olumide Adekanmbi, Olabisi Comfort Akinlabi, Adedolapo Victoria Olaposi
Summary: There is a rapid rise in the incidence of quinolone resistant bacteria in Nigeria. Most studies in Nigeria have focused on isolates from the clinical settings, with few focusing on isolates of environmental origin. This study aimed to investigate the antibiogram and carriage of plasmid-mediated quinolone resistance (PMQR) genes by quinolone-resistant isolates obtained from a pool of cefotaxime-resistant Escherichia coli (E. coli) recovered from sewage leaking out of some surface-leaking sanitary sewers in a University community in Nigeria. The findings showed a high level of resistance to antibiotics and carriage of PMQR genes by quinolone-resistant E. coli obtained from the leaking sanitary sewers, suggesting a potential environmental and public health concern.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Environmental Sciences
Xue Yu, Ying Zhang, Lu Tan, Chenglong Han, Haixiao Li, Lifang Zhai, Weiqi Ma, Chengtao Li, Xueqiang Lu
Summary: The study found that antibiotic resistance genes enriched on microplastics were more abundant than on inorganic substrates and natural water environments, but less abundant than on natural organic substrates. Microplastics in freshwater tended to have a higher degree of ARG enrichment than those in saline water and sewage.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Aerospace
V. K. Ilyin, O. Orlov, Yu A. Morozova, M. A. Skedina, S. K. Vladimirov, E. Plotnikov, A. A. Artamonov
Summary: This study presents a phenomenological model for the horizontal spread of bacterial drug resistance genes in spacecraft crews, based on experiments involving identifying microbial communities in the nasopharynx and determining gene transfer frequencies in space flight conditions. The model predicts a multiple decrease in antibiotic resistance in space conditions, while R-plasmids are expected to show increased resistance.
Article
Agriculture, Dairy & Animal Science
Dekang Zhu, Xinyi Wei, Hong Zhu, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Anchun Cheng
Summary: This study sequenced and transferred a plasmid from a multidrug-resistant strain of R. anatipestifer, and found that the plasmid contained several resistance genes which caused significant resistance to common antibiotics.
Article
Multidisciplinary Sciences
Mohammed Badrul Amin, Sumita Rani Saha, Md Rayhanul Islam, S. M. Arefeen Haider, Muhammed Iqbal Hossain, A. S. M. Homaun Kabir Chowdhury, Emily K. Rousham, Mohammad Aminul Islam
Summary: This study investigated the presence of PMQR genes in E. coli from various water sources in Bangladesh, finding that majority of isolates carried PMQR genes and were associated with multidrug resistance and resistance to extended spectrum beta-lactam antibiotics. The study highlights the widespread distribution of PMQR-positive MDR E. coli in aquatic environments of Bangladesh, indicating poor wastewater treatment and emphasizing the risk of transmission to humans and animals.
Article
Engineering, Environmental
Houpu Zhang, Jiajin Song, Zhiruo Zheng, Tongxin Li, Nan Shi, Yuling Han, Luqing Zhang, Yunlong Yu, Hua Fang
Summary: Co-pollution of soil with pesticide residues and antibiotic resistance genes (ARGs) is increasing due to the substantial usage of pesticides and organic fertilizers in greenhouse-based agricultural production. Non-antibiotic stresses, including those from agricultural fungicides, are potential co-selectors for the horizontal transfer of ARGs, but the underlying mechanism remains unclear.
Review
Infectious Diseases
Zineb Cherak, Lotfi Loucif, Abdelhamid Moussi, Jean-Marc Rolain
Summary: Colistin is a crucial last-line therapy for multidrug-resistant Gram-negative pathogens, yet resistance to it has become a major global public-health crisis. Plasmid-mediated mcr genes encoding enzymes are the main mechanism leading to colistin resistance, dispersed globally. Water environments play a crucial role in the spread of antibiotic resistance, acting as reservoirs and dissemination routes for mcr genes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Food Science & Technology
Hyeeun Park, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: PMQR genes are frequently detected in ESBL-producing E. coli isolates from retail raw chicken in South Korea, posing a public health concern.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Shu-Rui Li, Chun-Mei Zeng, Xin-Mei Peng, Jin-Ping Chen, Shuo Li, Cheng-He Zhou
Summary: A new type of benzopyrone-mediated quinolones (BMQs) was designed and synthesized as potential antibacterial molecules to combat drug resistance. Among the synthesized BMQs, ethylidene-derived BMQ 17a displayed superior antibacterial activity with low toxicity to human cells. Mechanistic investigation revealed that BMQ 17a induced bacterial metabolic disorders, increased reactive oxygen species, disrupted the bacterial antioxidant defense system, and caused cell death.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Xinhua Wang, Xianhui Jiao, Yao Fu, Wei Gao, Mingming Gao, Ranran Huang
Summary: This study investigated the influence of the antibiotic sulfadiazine (SDZ) on aerobic granular sludge (AGS) systems at decreasing temperature, revealing effects on granulation, granule structure, settleability, density, biomass, and nitrogen removal performance. The SDZ demonstrated hormesis effects on AGS, with faster granulation and stable granule structure at lower concentrations, and slower granule formation and severe disintegration at higher concentrations. Metagenomic analysis uncovered higher abundances of resistance genes and transposases in reactors with high SDZ concentrations, indicating a potential for ARGs exchange in AGS microbes.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Kaiqi Lu, Mingming Gao, Bo Sun, Mei Wang, Shuguang Wang, Xinhua Wang
Summary: This study established a coupling effect of Cr-Fenton-like reaction and Cr flocculation for simultaneous removal of Cr and organic pollutants. The formation of Cr flocs was crucial in providing heterogeneous catalytic regions for the coupling effect. Application of this coupling effect achieved high removal rates for Cr and organic carbon in simulated wastewater, presenting a sustainable and eco-friendly strategy for remediation.
Article
Microbiology
Sheng-Zhi Guo, Tong Wu, Hai-Zhen Zhu, Lei Yan, Zhi-Pei Liu, De-Feng Li, Cheng-Ying Jiang, Shuang-Jiang Liu, Xi-Hui Shen
Summary: Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains were isolated from the Dragon-shaped Wetland System in Beijing Olympic Park, PR China. They belong to the genera Niabella and Thermomonas, respectively.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Ruofan Yan, Yibing Wang, Jiahuan Li, Xinhua Wang, Yunkun Wang
Summary: The study found that different microorganisms have different contributions to the biodegradation pathways of sulfamethoxazole (SMX) and the removal of antibiotic resistant genes (ARGs). Heterotrophic bacteria play a significant role in SMX degradation, while ammonia-oxidizing bacteria (AOB) have a greater contribution to SMX removal through co-metabolism.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Gastroenterology & Hepatology
Rui Zhao, Zimai Li, Yuqing Sun, Wei Ge, Mingyu Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Hyperuricemia, the second most prevalent metabolic disease, can be effectively treated by introducing recombinant urate-degrading bacteria to enhance intestinal secretion, according to a study.
Article
Engineering, Environmental
Haoxuan Chen, Yunhao Zheng, Mingyu Wang, Yan Wu, Maosheng Yao
Summary: Breath-borne volatile organic compounds (VOCs) have been studied as biomarkers in medical diagnosis and environmental health research. In this study, the release of cellular VOCs from Saccharomyces cerevisiae after toxicant exposures and the underlying mechanism were investigated. The findings showed that different toxicant exposures caused distinctive VOC profiles in yeast cells. Ethyl acetate and ethyl n-propionate levels changed in response to all the toxicants tested, suggesting their potential for environmental toxicity monitoring. RNA-seq results revealed significant changes in metabolic and signaling pathways related to ribosome, carbohydrate, and amino acid metabolisms. These changes in metabolism may have contributed to the observed release of VOCs, such as ethyl acetate, from yeast cells. This study provides further evidence of the potential of VOCs released from living organisms for monitoring and protecting against toxic exposures, while also offering insights into the mechanistic changes in breath-borne VOCs observed in rats and humans exposed to air toxicants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Lei Yan, Ning Yan, Xi-Yan Gao, Ying Liu, Zhi-Pei Liu
Summary: A highly efficient AMX-degrading strain Ads-6, belonging to the genus Bosea, was isolated and characterized. Ads-6 exhibited strong AMX-degrading ability and showed great potential in the biodegradation of antibiotics and bioremediation of contaminated environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Lei Yan, Bin Liang, Meng-Yuan Qi, Ai-Jie Wang, Zhi-Pei Liu
Summary: In this study, a bacterial strain Nocardioides sp. N39 capable of degrading 3A5MI was isolated and its degradation ability was explored under different conditions. The strain showed the ability to degrade various analogs of 3A5MI as well. However, the degradation ability of N39 was not permanent and long-term storage resulted in the loss of this ability, possibly due to mobile genetic elements in the bacterium. Despite this, N39 provided valuable information about the degradation of 3A5MI and highlighted the importance of studying environmental effects and potential degradation mechanisms.
Article
Electrochemistry
Xue Zheng, Mingming Gao, Chen Liang, Shuguang Wang, Xinhua Wang
Summary: In this study, polyaniline (PAN) was used as an anchor to enhance the interaction between carbon materials and TiO2, leading to improved catalytic activity. The introduction of PAN resulted in a higher electrochemical activity and more active sites of TiO2, and also allowed for the preparation of a self-supporting electrode.
ELECTROCHIMICA ACTA
(2022)
Article
Engineering, Environmental
Weiwei Li, Zhongjun Yang, Jiamin Hu, Bianfang Wang, Hao Rong, Ziyun Li, Yuqing Sun, Yunkun Wang, Xuhua Zhang, Mingyu Wang, Hai Xu
Summary: This study investigated the extent of resistance to 'last-resort' antibiotics in hospital wastewater. The findings revealed high levels of resistance and prevalent multiple resistance to antibiotics, including 'last-resort' antibiotics. The study also discovered inconsistencies between resistance phenotypes and genotypes, as well as the first occurrence of tet(X4)-containing tigecycline-resistant Gram-positive pathogens. Furthermore, the wastewater treatment processes were effective in preventing the release of 'last -resort' antibiotic resistant pathogens.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Yuqing Zhang, Minchao Zha, Mingming Gao, Xinhua Wang
Summary: This study applied a static magnetic field to accelerate the granulation of microalgal-bacterial granular sludge (MBGS) in photo-sequencing batch reactors. The magnetic field significantly promoted microbial growth, sludge settleability, and granule density, leading to rapid granulation and improved performance in MBGS. The magnetic field also enhanced nitrogen removal and increased the specific oxygen production rate and specific oxygen uptake rate in MBGS. The accumulation of metal ions, production of extracellular polymeric substances, and enrichment of functional bacteria and phototrophs contributed to the successful granulation of MBGS. This study provides an effective and environmentally-friendly method for rapid cultivation of MBGS.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Electrochemistry
Wen Zhang, Manshu Zhao, Xinhua Wang, Shuguang Wang, Mingming Gao
Summary: This study investigates the behavior of graphite supported TiO2 in anodic oxidation. It is found that the TiO2/C anode generates H2O2 as an intermediate, which then indirectly decomposes to produce center dot OH. At higher potentials, the direct decomposition of H2O is the main source of OH generation, but it is inhibited by high concentrations of H2O2. The relationship between oxygen defects and center dot OH generation is experimentally established, and the mechanisms of OH generation are correlated with oxygen vacancies and Ti-OH structures.
ELECTROCHIMICA ACTA
(2022)
Article
Biology
Xin Wei, Jian Zhang, Bianfang Wang, Wenjia Wang, Yuqing Sun, Ling Li, Hai Xu, Mingyu Wang
Summary: The emergence of antibiotic resistance in bacteria poses a significant threat to human health. Antibiotic resistance genes (ARGs) are widely present in the environment and are consumed daily by humans. It has been suspected that uptake of ARGs may lead to increased antibiotic resistance in bacteria colonizing human bodies. This study confirmed that both antibiotics and ARGs can increase antibiotic resistance levels, but this response is limited in time and space. The findings highlight the importance of addressing antibiotic resistance gene pollution and antibiotic misuse.
Article
Engineering, Environmental
Huiting Chen, Xiaoxi Wu, Ling Li, Mingyu Wang, Chao Song, Shuguang Wang, Zhen Yan
Summary: This study reports the use of extracellular carbonic anhydrase as a biomarker for monitoring antibiotics. The enzyme was successfully overexpressed using a recombinant microbial factory, and its feasibility in monitoring antibiotics in vitro and in vivo was confirmed.
JOURNAL OF HAZARDOUS MATERIALS LETTERS
(2022)
Article
Microbiology
Weiwei Li, Jiamin Hu, Ling Li, Mengge Zhang, Qingyu Cui, Yanan Ma, Hainan Su, Xuhua Zhang, Hai Xu, Mingyu Wang
Summary: This study identified a multidrug-resistant Enterococcus faecium strain EF332 that is resistant to both daptomycin and vancomycin, and discovered new genetic mutations that confer daptomycin resistance.
FRONTIERS IN MICROBIOLOGY
(2022)