期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep43564
关键词
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资金
- French Infrastructure for Integrated Structural Biology Initiative FRISBI [ANR-10-INSB-05-02]
- Grenoble Alliance for Integrated Structural Cell Biology GRAL [ANR-10-LABX-49-01]
- Agence Nationale de la Recherche [ANR-11-BSV8-005-01 PILIPATH, ANR-12-BSV3-0003-01 PVL-Suscept]
- Commissariat a l'Energie Atomique (Fonds de soutien technologique a la valorisation des recherches de DSV)
- University of Grenoble Alpes
- Grenoble Alpes University
Unusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 angstrom. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.
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