Article
Infectious Diseases
Vladimir Gostev, Olga Kalinogorskaya, Julia Sopova, Ofelia Sulian, Polina Chulkova, Maria Velizhanina, Irina Tsvetkova, Irina Ageevets, Vladimir Ageevets, Sergey Sidorenko
Summary: Vancomycin and daptomycin are commonly used antibiotics for treating methicillin-resistant Staphylococcus aureus infections, but their efficacy is limited by their resistance and associated resistance to each other. The effectiveness of novel lipoglycopeptides in overcoming this associated resistance is still unknown.
Article
Biochemical Research Methods
Yu-Ching Liu, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chuan Lin, Chao-Jung Chen
Summary: The rapid identification of methicillin-sensitive Staphylococcus aureus (MSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-intermediate S. aureus (VISA) is crucial for accurate treatment, timely intervention, and outbreak prevention. Proteomics analysis of 90 S. aureus isolates led to the discovery of protein biomarkers that can discriminate among MSSA, hVISA, and VISA strains, with two VISA, two hVISA, and one MSSA protein biomarkers identified and verified for high sensitivities and specificities. Data is available via MassIVE with identifier MSV000085776.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Infectious Diseases
Reena Lamichhane-Khadka, Santosh Dulal, Jesus A. Cuaron, Richard Pfeltz, Sushim Kumar Gupta, Brian J. Wilkinson, John E. Gustafson
Summary: Comparative genomic sequencing of two laboratory-derived vancomycin-intermediate Staphylococcus aureus (VISA) strains (MM66-3 and MM66-4) revealed unique mutations compared to the parent strain MM66. Transcriptional profiling showed similar changes in both VISA strains, including reduced autolysis, growth in the presence of salt, and virulence factor activity. Mutations in the key gene apt may play a role in altered adenine metabolism in VISA strains.
Article
Microbiology
Shawna McCallin, Carmen Menzi, Swenja Lassen, Jean Daraspe, Frank Oechslin, Philippe Moreillon
Summary: In Vancomycin-intermediate Staphylococcus aureus (VISA), exposure to vancomycin may lead to cross-resistance to phage. VISA strains with intermediate levels of vancomycin resistance showed decreased sensitivity to phage. Serial passaging with vancomycin induced reduced vancomycin susceptibility and phage sensitivity. Understanding the interference between antibiotic and phage resistance mechanisms is important for designing effective therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Yanlei Xu, Bingjie Wang, Huilin Zhao, Xinyi Wang, Lulin Rao, Wenxiu Ai, Jingyi Yu, Yinjuan Guo, Xiaocui Wu, Fangyou Yu, Shuying Chen
Summary: The study found that all MRSA isolates remained sensitive to vancomycin, teicoplanin, linezolid and daptomycin, but an increase in MIC50 and MIC90 was observed for linezolid.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Jang Ho Lee, Myeong Geun Choi, Hyung Jun Park, Ho Cheol Kim, Chang-Min Choi
Summary: This study compared the efficacy and safety of vancomycin and teicoplanin in patients with methicillin-resistant Staphylococcus aureus pneumonia. The results showed that vancomycin had lower clinical failure rates, discontinuation rates due to treatment failure, and mortality rates compared to teicoplanin. However, it had higher rates of side effects. Therefore, clinicians need to consider the efficacy and potential side effects of these drugs before prescribing.
BMC INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Michelle Su, Michelle H. Davis, Jessica Peterson, Claudia Solis-Lemus, Sarah W. Satola, Timothy D. Read
Summary: VISA resistance is typically caused by accumulation of chromosomal mutations in six common genes, but the effect of these mutations on fitness and resistance contribution varies among different strain backgrounds.
Article
Microbiology
Alina Dietrich, Ursula Steffens, Mike Gajdiss, Anna-Lena Boschert, Jana Katharina Droege, Christiane Szekat, Peter Sass, Imran T. Malik, Jan Bornikoel, Laura Reinke, Boris Macek, Mirita Franz-Wachtel, Kay Nieselt, Theresa Harbig, Kirstin Scherlach, Heike Broetz-Oesterhelt, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Summary: Resistance to antibiotics is a growing problem and alternative antimicrobial therapies are needed. Cervimycin, a polyketide antibiotic, shows promising activity against multidrug-resistant staphylococci and vancomycin-resistant enterococci. This study identified the Clp system and the essential kinase WalK as mutational hotspots for cervimycin resistance in Staphylococcus aureus. Furthermore, cervimycin-resistant strains exhibited vancomycin-intermediate resistance phenotype, suggesting a role of cervimycin in perturbing the bacterial cell envelope.
MICROBIOLOGY SPECTRUM
(2022)
Article
Public, Environmental & Occupational Health
Iqra Muzammil, Muhammad Ijaz, Muhammad Hassan Saleem, Muhammad Muddassir Ali
Summary: This study aimed to investigate the molecular prevalence of VRSA in bovine milk samples, as well as risk factors, antimicrobial susceptibility, and bioinformatics analysis targeting the vanB gene. Among 248 S. aureus isolates, the estimated prevalence of VRSA was 17.74% phenotypically and 10.89% genotypically. Farm-level risk factors, including improper milking technique and lack of hygiene during milking, were significantly associated with VRSA infection. VRSA isolates showed resistance to oxytetracycline, oxacillin, and Trimethoprim+sulfamethoxazole. The study also identified conserved residues and potential breakpoints in the vanB gene using bioinformatics tools. The presence of VRSA in animals poses a zoonotic threat and requires serious attention for consumer health.
ZOONOSES AND PUBLIC HEALTH
(2023)
Article
Infectious Diseases
Deniz Gazel, Mehmet Erinmez, Ayse Buyuktas Manay, Yasemin Zer
Summary: Our investigation revealed higher rates of hVISA compared to other studies in Turkey, while both VRE agar and BHIAV screening tests failed to detect hVISA properly. The combination of meropenem and vancomycin inhibited 90% of hVISA isolates in our study.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2021)
Article
Multidisciplinary Sciences
Yifan Rao, Huagang Peng, Weilong Shang, Zhen Hu, Yi Yang, Li Tan, Ming Li, Renjie Zhou, Xiancai Rao
Summary: The WalK(S221P) mutation enhances vancomycin resistance and decreases virulence in Staphylococcus aureus.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Medicine, General & Internal
Susmita Unni, Tahseen J. Siddiqui, Satesh Bidaisee
Summary: Staphylococcus aureus infections, especially those resistant to vancomycin, are increasingly reported globally, raising concerns. Active surveillance, strict infection control measures, and appropriate antibiotic use are crucial in reducing the spread of these resistant strains.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Microbiology
Jiade Zhu, Banghui Liu, Xueqin Shu, Baolin Sun
Summary: Through comparative genome analysis, this study identified 10 mutations in 9 genes and verified the role of a novel mutation, WalK (I237T), in conferring VISA characteristics in MSSA. The mutation resulted in thickened cell walls, reduced autolysis, attenuated virulence, and altered gene expression associated with cell wall metabolism and virulence control. Further assays showed that WalK can directly bind to specific promoter regions and regulate gene expression, providing insight into the molecular mechanisms of developmental VISA resistance in MSSA with point mutations.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Microbiology
Abraham G. Moller, Robert A. Petit III, Michelle H. Davis, Timothy D. Read
Summary: This study developed a method for rapid detection of vancomycin-intermediate resistance in Staphylococcus aureus through long-read genomic sequencing. The method can identify VISA genotypes and incorporate a comprehensive database of VISA genetic determinants.
MICROBIOLOGY SPECTRUM
(2023)