期刊
SCIENTIFIC REPORTS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep42398
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [3306, 23115003, 23115001]
- JST-SENTAN program
- JSPS Core-to-Core Program, A. Advanced Research Networks
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Wellcome Trust [WT WT098519MA]
- Royal Society International Joint project grant
- [16J00111]
- Grants-in-Aid for Scientific Research [16H02617, 26310209, 16K14555, 25253016, 16J00111, 15H05901, 15H05897] Funding Source: KAKEN
We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluorescence from environment severely interferes fluorescence imaging. Through imaging of moving cardiomyocyte aggregates, we demonstrated the advantages of LOTUS-V in long-term imaging are attributable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with the ratiometric aspect of LOTUS-V design. Collectively LOTUS-V extends the scope of excitable cell control and simultaneous voltage phenotyping, which should enable applications in bioscience, medicine and pharmacology previously not possible.
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