Article
Neurosciences
Nevin Varghese, Nicolas E. Amelinez-Robles, Barclay Morrison III
Summary: In this study, the effects of blast-induced traumatic brain injury (bTBI) on the hippocampal network of rat organotypic hippocampal slice cultures (OHSCs) were investigated. The results showed reduced connectivity and clustering coefficients in the hippocampal network following bTBI, along with decreased expression of alpha 1 and alpha 5 GABAA receptor subunits. Treatment with the histone deacetylase inhibitor suberanilohydroxamic acid (SAHA) restored the alpha 1 subunit and attenuated deficits in network measures. These findings suggest that GABAA receptors may play a role in neuronal network changes and could be targeted for therapeutic intervention in bTBI-induced neurological symptoms.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Virology
Lise Lamoureux, Babu Sajesh, Jessy A. Slota, Sarah J. Medina, Matthew Mayor, Kathy L. Frost, Bryce Warner, Kathy Manguiat, Heidi Wood, Darwyn Kobasa, Stephanie A. Booth
Summary: Neurological syndromes associated with COVID-19 suggest an effect of the virus on neuronal function, but the direct infection of SARS-CoV-2 in the brain remains under debate. In this study, using brain slice culture, researchers found that while some glial cells were infected, there was no evidence of viral infection or replication in neurons. These findings support previous clinical studies on neurological involvement in COVID-19 patients.
Review
Biochemistry & Molecular Biology
Lin He, Chuxia Deng
Summary: Organotypic tissue slice culture has numerous advantages in anticancer drug development, including the preservation of cell repertoire, quick assessment of therapeutic efficacy, and the ability to select personalized treatment regimens. It is expected to become a potent platform for individualized drug screening.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Susanna Kemppainen, Nadine Huber, Roosa-Maria Willman, Ana Zamora, Petra Makinen, Henna Martiskainen, Mari Takalo, Annakaisa Haapasalo, Tomas Sobrino, Manuel Antonio Gonzalez Gomez, Yolanda Pineiro, Jose Rivas, Uwe Himmelreich, Mikko Hiltunen
Summary: Organotypic slice culture models, especially those derived from adult tissue, provide a valuable tool for studying neurodegenerative diseases such as tauopathies. In this study, hippocampal slice cultures from adult transgenic mice were used to investigate the effects of a novel antibody on hyperphosphorylated TAU. The adult-originating slices retained key cellular components and exhibited tau pathology, making them a suitable model for studying TAU pathology in the adult brain without the interference of the blood-brain barrier.
Article
Multidisciplinary Sciences
James M. McCarthy, Jasmeet Virdee, Jessica Brown, Daniel Ursu, Zeshan Ahmed, Annalisa Cavallini, Hugh N. Nuthall
Summary: Intracellular tau inclusions are a hallmark of several neurodegenerative diseases, and recent studies have shown that tau aggregates can propagate to neighboring cells, leading to further tau aggregation. The ex vivo system using organotypic hippocampal slice cultures (OHCs) provides a model for studying this phenomenon and assessing potential therapeutic approaches.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ingrid Zahn, Fabian Garreis, Martin Schicht, Vera Roetzer, Jens Waschke, Yuqiuhe Liu, Valerian L. Altersberger, Friedrich Paulsen, Jana Dietrich
Summary: A new 3D organotypic slice culture model of mouse meibomian glands (MGs) was established, which allows for the study of meibomian gland dysfunction (MGD) and dry eye disease (DED). The effects of melanocortins on exocrine secretion were investigated, potentially leading to new insights in the development of treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Sanjiban Chakrabarty, William F. Quiros-Solano, Maayke M. P. Kuijten, Ben Haspels, Sandeep Mallya, Calvin Shun Yu Lo, Amr Othman, Cinzia Silvestri, Anja van de Stolpe, Nikolas Gaio, Hanny Odijk, Marieke van de Ven, Corrina M. A. de Ridder, Wytske M. van Weerden, Jos Jonkers, Ronald Dekker, Nitika Taneja, Roland Kanaar, Dik C. van Gent
Summary: This study developed a microfluidic platform for evaluating cancer treatment response and predicting drug efficacy in breast and prostate tumors. By culturing tumor slices under controlled growth conditions, the platform allows reliable assessment of chemotherapeutic sensitivity and enables detailed mechanistic studies.
Article
Chemistry, Multidisciplinary
Eunhee Kim, Sungwoong Jeon, Yoon-Sil Yang, Chaewon Jin, Jin-young Kim, Yong-Seok Oh, Jong-Cheol Rah, Hongsoo Choi
Summary: The magnetic neurospheroid (Mag-Neurobot) can establish both structural and functional connections with an organotypic hippocampal slice (OHS), providing a new research direction and test platform for functional restoration in cell therapy and regenerative medicine.
ADVANCED MATERIALS
(2023)
Article
Immunology
Marina da Silva Oliveira, Larissa Marcely Gomes Cassiano, Jeanne Pioline, Ketyllen Reis Andrade de Carvalho, Anna Christina de Matos Salim, Pedro Augusto Alves, Gabriel da Rocha Fernandes, Alexandre de Magalhaes Vieira Machado, Roney Santos Coimbra
Summary: This study evaluated how two Brazilian Zika virus (ZIKV) isolates with subtle genetic differences impact hippocampal infection and host response, and found that these differences can also lead to long-term effects on neuronal population.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Multidisciplinary Sciences
Simone Nardin Weis, Jaques Miranda F. Souza, Juliana Bender Hoppe, Marina Firmino, Manfred Auer, Nassim N. Ataii, Leonardo Assis da Silva, Mariana Maier Gaelzer, Caroline Peres Klein, Alan R. Mol, Consuelo M. R. de Lima, Diogo Onofre Souza, Christianne G. Salbego, Carlos Andre O. Ricart, Wagner Fontes, Marcelo Valle de Sousa
Summary: Research has shown that there are fundamental sex-related differences in the proteome of organotypic hippocampal slice culture, with females exhibiting a greater prevalence of astrocytic metabolism and males showing significant neuronal metabolism. These differences manifest in preferences for glucose use, mitochondrial respiration subunits, and lipid metabolism between the sexes.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Ari Ogaki, Yuji Ikegaya, Ryuta Koyama
Summary: Researchers have developed a method to efficiently transplant human induced pluripotent stem cell-derived microglia (hiPSC-MG) into mouse hippocampal slice cultures, achieving a high replacement rate. The transplanted microglia changed their morphology and phagocytosed cell debris when neuronal death was induced. This method provides a useful ex vivo tool for evaluating the properties of hiPSC-MG.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Davide Maselli, Rolando S. Matos, Robert D. Johnson, Davide Martella, Valeria Caprettini, Ciro Chiappini, Patrizia Camelliti, Paola Campagnolo
Summary: This paper introduces a novel model, 3D organotypic heart slices, for studying epicardial physiology and activation, derived from porcine hearts and retaining a healthy epicardial layer. These slices provide an ideal platform that mimics the real environment in the lab, suitable for studying epicardial physiology or performing pharmacological interventions/gene therapy approaches.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Kyung Hee Lee, Un Jeng Kim, Myeounghoon Cha, Bae Hwan Lee
Summary: Increased oxidative damage in the brain with age causes abnormal brain function and disease. Ascorbic acid is an endogenous antioxidant that protects neurons, but its levels decrease with aging. Studies show that chronic ascorbate supplementation can reduce neuronal death and preserve the antioxidant system in the brain, protecting hippocampal neurons from age-related oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Vanessa Etschmaier, Muammer Uecal, Birgit Lohberger, Markus Absenger-Novak, Dagmar Kolb, Annelie Weinberg, Ute Schaefer
Summary: An ex vivo rat femur slice model was used to investigate the mechanisms of growth impairment following trauma-related bone fractures. The study showed disrupted endochondral ossification, altered expression patterns of specific genes, and increased chondrocyte metabolic activity in the injured area. The presence of stem cells, fibroblasts, and chondrocytes was also observed at the injury site. These findings suggest that the ex vivo organotypic GP injury model is valuable for studying GP regeneration post-trauma and other tissue engineering and disease studies.
Article
Multidisciplinary Sciences
Amy McCaughey-Chapman, Bronwen Connor
Summary: Organotypic brain slice cultures provide a 3-dimensional system for studying neurological diseases. By treating rat sagittal organotypic slices with AMPA or quinolinic acid (QA), researchers have developed ex vivo excitotoxic lesion models that mimic the in vivo process. This slice culture platform allows for long-term cellular studies and provides a valuable tool in understanding excitotoxicity and developing therapeutic strategies.
Review
Pharmacology & Pharmacy
Martin Madill, Denise Fitzgerald, Kara E. O'Connell, Kumlesh K. Dev, Sanbing Shen, Una FitzGerald
DRUG DISCOVERY TODAY
(2016)
Article
Neurosciences
Min Liu, Zhenlong Guan, Qin Shen, Pierce Lalor, Una Fitzgerald, Timothy O'Brien, Peter Dockery, Sanbing Shen
JOURNAL OF NEUROSCIENCE
(2016)
Editorial Material
Cell Biology
Michelle C. Naughton, Jill M. McMahon, Una F. FitzGerald
NEURAL REGENERATION RESEARCH
(2016)
Article
Neurosciences
Min Liu, Ping Xu, Zhenlong Guan, Xiaohong Qian, Peter Dockery, Una Fitzgerald, Timothy O'Brien, Sanbing Shen
Article
Neurosciences
Enrico Bagnoli, Una FitzGerald
EUROPEAN JOURNAL OF NEUROSCIENCE
(2019)
Article
Neurosciences
Michelle Naughton, Jill McMahon, Sinead Healy, Una FitzGerald
JOURNAL OF COMPARATIVE NEUROLOGY
(2019)
Article
Neurosciences
Enrico Bagnoli, Tara Diviney, Una FitzGerald
Summary: DOPAL has a direct toxic effect on astrocytes, leading to decreased mitochondrial function, increased oxidative stress, and impaired cell viability.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Engineering, Biomedical
Naledi Shologu, Mehmet Gurdal, Eva Szegezdi, Una FitzGerald, Dimitrios I. Zeugolis
Summary: This study evaluated the potential of macromolecular crowding in improving cell-derived matrices for in vitro tumor models. NP40 was the most effective decellularisation protocol in removing cellular matter while preserving the deposited matrix. Macromolecular crowding derived matrices produced by mammary fibroblasts showed increased adhesion molecules, metalloproteinases, and proinflammatory cytokines, as well as enhanced resistance to doxorubicin and reduced cell death caused by reactive oxygen species.
Article
Chemistry, Multidisciplinary
Alexandre Trotier, Enrico Bagnoli, Tomasz Walski, Judith Evers, Eugenia Pugliese, Madeleine Lowery, Michelle Kilcoyne, Una Fitzgerald, Manus Biggs
Summary: The development of bioelectronic neural implant technologies, particularly in brain-machine interfaces and electronic medicine, has made significant progress in the past 5 years. However, invasive neurosurgery and mechanical shear caused by micromotion can lead to chronic inflammation, void formation and glial scar tissue deposition, hindering signal propagation and reducing the functionality of neural implants. The mechanisms behind these processes are still poorly understood.
Article
Biochemistry & Molecular Biology
Enrico Bagnoli, Alexandre Trotier, Jill McMahon, Leo R. Quinlan, Manus Biggs, Abhay Pandit, Una Fitzgerald
Summary: Parkinson's disease is a progressive neurodegenerative disorder with unknown etiology and no cure. The olfactory bulb, involved in processing olfactory information, is affected early in the disease. The study demonstrates that dopamine metabolite DOPAL can replicate Parkinson's pathology, shedding light on the disease mechanism.
Review
Cell Biology
Robert F. Hillary, Una FitzGerald
JOURNAL OF BIOMEDICAL SCIENCE
(2018)
Article
Sport Sciences
Mark Ganly, Jill Mary McMahon
BMJ OPEN SPORT & EXERCISE MEDICINE
(2018)