Article
Multidisciplinary Sciences
Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takahiro Kawagishi, Jeffery A. Nurdin, Moeko Yamasaki, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, Takeshi Kobayashi
Summary: Reverse genetics systems are powerful tools for investigating viral gene function and vaccine development. This study developed a plasmid-based reverse genetics system for Tarumizu tick virus (TarTV) of the genus Coltivirus, enhancing understanding of the replication machinery of the Reoviridae family.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Yuta Kanai, Takeshi Kobayashi
Summary: Reverse genetics systems allow the generation of gene-engineered recombinant viruses for studying the roles of viral genes and proteins in infectivity, replication, and pathogenicity. Rotavirus was previously refractory to reverse genetics analysis until the establishment of a complete system in 2017 using the fusogenic protein FAST for rescue.
ANNUAL REVIEW OF VIROLOGY, VOL 8
(2021)
Review
Immunology
Vicent Tur-Planells, Adolfo Garcia-Sastre, Sara Cuadrado-Castano, Estanislao Nistal-Villan
Summary: This review provides an overview of non-human-adapted RNA viruses used in cancer therapy and the diverse strategies employed to optimize their therapeutic capabilities. Through this article, we gain insights into the promising advancements in virotherapy and its potential to revolutionize cancer treatment.
Review
Virology
Xinyu Zhang, Feng Wen
Summary: Reovirales are a group of viruses that pose a serious threat to human and animal health, but the study of their biology has lagged behind due to the lack of an effective reverse genetics system. Recently, plasmid-based reverse genetics systems have been developed for some Reovirales viruses.
Article
Multidisciplinary Sciences
Fabien Aubry, Sofie Jacobs, Mailis Darmuzey, Sebastian Lequime, Leen Delang, Albin Fontaine, Natapong Jupatanakul, Elliott F. Miot, Stephanie Dabo, Caroline Manet, Xavier Montagutelli, Artem Baidaliuk, Fabiana Gambaro, Etienne Simon-Loriere, Maxime Gilsoul, Claudia M. Romero-Vivas, Van-Mai Cao-Lormeau, Richard G. Jarman, Cheikh T. Diagne, Oumar Faye, Ousmane Faye, Amadou A. Sall, Johan Neyts, Laurent Nguyen, Suzanne J. F. Kaptein, Louis Lambrechts
Summary: The study finds that recent African ZIKV strains have higher transmissibility in mosquitoes and higher lethality in both adult and fetal mice when compared to their Asian counterparts. The authors emphasize the high epidemic potential of African ZIKV strains, suggesting that they may more easily go unnoticed by public health surveillance systems due to their tendency to cause fetal loss rather than birth defects.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Sheng-Chieh Lin, Geng-Hao Bai, Pei-Chun Lin, Chung-Yung Chen, Yi-Hsiang Hsu, Yuan-Chang Lee, Shih-Yen Chen
Summary: Human noroviruses (HuNoV) are a major cause of acute gastroenteritis worldwide, and their high mutation rate and recombination potential pose challenges in studying their genetic diversity and evolution. Recent advances in technology have allowed for the detection and analysis of complete genome sequences of noroviruses, as well as the tracing of their evolution and genetic diversity. The inability to cultivate infectious viruses in cell models has hindered the understanding of HuNoV infection mechanisms and the development of antiviral drugs. However, reverse genetics has shown promise in recovering and generating infectious viral particles, providing an alternative method for studying viral infection mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Ola Diebold, Victoria Gonzalez, Luca Venditti, Colin Sharp, Rosemary A. Blake, Wenfang S. Tan, Joanne Stevens, Sarah Caddy, Paul Digard, Alexander Borodavka, Eleanor Gaunt
Summary: We explored the use of rotaviruses (RVs) to express heterologous peptides, using SARS-CoV-2 as an example. Small SARS-CoV-2 peptide insertions (<34 amino acids) into the hypervariable region of the viral protein 4 (VP4) of RV SA11 strain resulted in reduced viral titer and replication, demonstrating a limited tolerance for peptide insertions at this site. To test the RV RF strain for its tolerance for peptide insertions, we constructed a reverse genetics system. NSP3 was C-terminally tagged with SARS-CoV-2 spike peptides of up to 193 amino acids in length. With a T2A-separated 193 amino acid tag on NSP3, there was no significant effect on the viral rescue efficiency, endpoint titer, and replication kinetics. Tagged NSP3 elicited cross-reactivity with SARS-CoV-2 spike antibodies in ELISA. We highlight the potential for development of RV vaccine vectors targeting multiple enteric pathogens simultaneously.
JOURNAL OF VIROLOGY
(2022)
Review
Virology
Yuta Kanai, Takeshi Kobayashi
Summary: Rotaviruses cause acute gastroenteritis in infants and young children. Since 2006, live-attenuated vaccines have reduced the number of deaths, but RV still causes estimated 228,047 annual deaths worldwide. A helper virus-free reverse genetics system for RV was established in 2017, leading to generation of recombinant RVs and verifying the robustness of reverse genetics systems.
Article
Immunology
Ahmad Jawad Sabir, Prince Pal Singh, Ivan Trus, Nguyen Phuong Khanh Le, Uladzimir Karniychuk
Summary: The 2015 Zika virus epidemic exhibited an unusual characteristic of fetal infection. The Asian Zika virus obtained African lineage mutations during in utero infection, which resulted in higher virus loads in the fetal brain. These findings raise concerns about the persistence of Zika virus and its potential for more aggressive infection phenotypes.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Pharmacology & Pharmacy
Sook Kwan Brown, Yeu-Yang Tseng, Ammar Aziz, Mariana Baz, Ian G. Barr
Summary: This study tested 3425 influenza B viruses collected from the Asia-Pacific region against four registered neuraminidase inhibitors (NAIs). It identified 45 influenza B viruses with reduced susceptibility to one or more NAIs, including some with newly identified neuraminidase mutations. The study highlights the potential impact of these mutations on the clinical effectiveness of NAIs in treating influenza B infections in humans.
ANTIVIRAL RESEARCH
(2022)
Review
Virology
Wenhao Wang, Xiaoxue Peng, Yunyun Jin, Ji-An Pan, Deyin Guo
Summary: The ongoing COVID-19 pandemic has led to severe public health crises and economic losses. Limited knowledge about the SARS-CoV-2 virus calls for more research. Reverse genetics systems, like viral infectious clones and replicons, provide powerful platforms for various viral research projects. The development of noninfectious replicons allows for safer operations in more widely available Biosafety Level 2 (BSL2) facilities. After the outbreak, multiple reverse genetics systems for SARS-CoV-2 have been developed, offering researchers various options for their studies.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Parasitology
Serena Marchi, Filippo Dragoni, Adele Boccuto, Olubukola T. Idoko, Maurizio Zazzi, Samba Sow, Aldiouma Diallo, Simonetta Viviani, Emanuele Montomoli, Ilaria Vicenti, Claudia Maria Trombetta
Summary: Genetic and phylogenetic studies have found that Zika virus (ZIKV) has two major lineages, African and Asian, but it is described as a single serotype. This study evaluated the cross-neutralization between ZIKV strains from the African and Asian lineages. The results showed that samples with neutralizing antibodies against the African lineage strain also had neutralizing activity against the Asian lineage strain, although with lower titers. The differences in neutralizing activity were attributed to approximately 120 amino acid differences between the two strains. However, all samples still had neutralizing antibody titers considered to be protective.
Article
Biochemistry & Molecular Biology
Julien Melade, Geraldine Piorkowski, Franck Touret, Toscane Fourie, Jean-Selim Driouich, Maxime Cochin, Hawa Sophia Bouzidi, Bruno Coutard, Antoine Nougairede, Xavier de Lamballerie
Summary: This study describes a simple method using infectious subgenomic amplicons (ISA) technology to generate recombinant infectious coronaviruses without the need for reconstruction of the complete genomic cDNA. This method has been successfully applied to SARS-CoV-2 and feline enteric coronavirus, enabling research on coronavirus reverse genetics, exploration of the molecular biological properties of SARS-CoV-2 variants, and acceleration of the development of effective therapeutic reagents.
Article
Pharmacology & Pharmacy
Chenxi Li, Xuan Chen, Yanyang Zhou, Jingbo Hu, Xinjie Wang, Yanhua Li
Summary: This study established a yeast artificial chromosome-based reverse genetics system for Japanese encephalitis virus (JEV) and developed an image-based antiviral assay using recombinant reporter viruses. Two broad-spectrum antiviral drugs were identified to have inhibitory effects on multiple genotypes of JEV.
ANTIVIRAL RESEARCH
(2022)
Article
Microbiology
Tey Putita Ou, Heidi Auerswald, Saraden In, Borin Peng, Senglong Pang, Sebastien Boyer, Rithy Choeung, Myrielle Dupont-Rouzeyrol, Philippe Dussart, Veasna Duong
Summary: Studies have shown that the African ZIKV strain exhibits higher pathogenicity in mosquito and mammalian cells, as well as in Ae. aegypti mosquitoes and mice. The transmission and survival rates of Ae. aegypti from rural habitats were significantly higher than those from urban areas. The African ZIKV isolate and the Cambodian ZIKV isolate demonstrated the highest transmission efficiency in the study.
Article
Microbiology
Remi Charrel, Ramona Mogling, Suzan Pas, Anna Papa, Cecile Baronti, Marion Koopmans, Herve Zeller, Isabelle Leparc-Goffart, Chantal B. Reusken
JOURNAL OF CLINICAL MICROBIOLOGY
(2017)
Article
Infectious Diseases
Josee Castonguay-Vanier, Raphaelle Klitting, Onanong Sengvilaipaseuth, Geraldine Piorkowski, Cecile Baronti, Bountoy Sibounheuang, Manivanh Vongsouvath, Anisone Chanthongthip, Soulignasack Thongpaseuth, Mayfong Mayxay, Koukeo Phommasone, Phouvieng Douangdala, Saythong Inthalath, Phouthalavanh Souvannasing, Paul N. Newton, Xavier de Lamballerie, Audrey Dubot-Peres
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Pharmacology & Pharmacy
Franck Touret, Cecile Baronti, Olivia Goethals, Marnix Van Loock, Xavier De lamballerie, Gilles Querat
ANTIVIRAL RESEARCH
(2019)
Article
Virology
Michele Bengue, Pauline Ferraris, Cecile Baronti, Cheikh Tidiane Diagne, Loic Talignani, Sineewanlaya Wichit, Florian Liegeois, Catherine Bisbal, Antoine Nougairede, Dorothee Misse
Article
Virology
Fode Diop, Haoues Alout, Cheikh Tidiane Diagne, Michele Bengue, Cecile Baronti, Rodolphe Hamel, Loic Talignani, Florian Liegeois, Julien Pompon, Ronald E. Morales Vargas, Antoine Nougairede, Dorothee Misse
Article
Infectious Diseases
Chantal Reusken, Cecile Baronti, Ramona Mogling, Anna Papa, Katrin Leitmeyer, Remi N. Charrel
Article
Cell Biology
Bruno Coutard, Jean-Louis Romette, Kathryn Miyauchi, Remi Charrel, Christine M. A. Prat
BIOPRESERVATION AND BIOBANKING
(2020)
Article
Biochemistry & Molecular Biology
Tuniyazi Abuduaini, Vincent Roy, Julien Marlet, Catherine Gaudy-Graffin, Denys Brand, Cecile Baronti, Franck Touret, Bruno Coutard, Tamara R. McBrayer, Raymond F. Schinazi, Luigi A. Agrofoglio
Summary: Novel 1,4-disubstituted-1,2,3-triazol-(E)-2-methyl-but-2-enyl nucleosides phosphonate prodrugs with 4-substituted-1,2,3-triazoles were synthesized through olefin acyclic cross metathesis. Compound 15j, a HDP/POC prodrug, exhibited significant activity against HBV in Huh7 cell cultures, with 62% inhibition at 10 μM.
Article
Microbiology
Franck Touret, Lea Luciani, Cecile Baronti, Maxime Cochin, Jean-Selim Driouich, Magali Gilles, Laurence Thirion, Antoine Nougairede, Xavier de Lamballerie
Summary: The new variant of the novel coronavirus, 20I/501Y.V1, has demonstrated a higher replication efficiency in human cellular models, potentially explaining its rapid spread in human populations.
Article
Biochemical Research Methods
C. Baronti, B. Coutard, X. de Lamballerie, R. Charrel, F. Touret
Summary: In the context of the COVID-19 pandemic, virus collections like EVA-GLOBAL play a crucial role in providing viruses and related products for research. Freeze-drying techniques are proven to be an effective method for preserving strains without the need for a cold chain, with sucrose serving as an adjuvant to enhance long-term preservation and shipment under various climatic conditions.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Anne Weiss, Franck Touret, Cecile Baronti, Magali Gilles, Bruno Hoen, Antoine Nougairede, Xavier de Lamballerie, Morten O. A. Sommer
Summary: Niclosamide has been validated as a potent antiviral agent against SARS-CoV-2 in human airway models, demonstrating efficacy against multiple variants. This highlights its potential as a therapeutic agent for COVID-19.
Article
Multidisciplinary Sciences
Eva Lopez, Margot Barthelemy, Cecile Baronti, Shirley Masse, Alessandra Falchi, Fabien Durbesson, Renaud Vincentelli, Xavier de Lamballerie, Remi Charrel, Bruno Coutard
Summary: A rapid molecular assay has been developed to detect mutations in variants of the COVID-19 virus, showing high sensitivity and specificity. This method can be used for high-throughput identification of mutations and serve as an initial screening to select samples for full genome sequencing.
Article
Immunology
Carlo Fischer, Celia Pedroso, Alfredo Mendrone, Ana Maria Bispo de Filippis, Antonio Carlos Rosario Vallinoto, Bergmann Morais Ribeiro, Edison Luiz Durigon, Ernesto T. A. Marques, Gubio S. Campos, Isabelle F. T. Viana, Jose Eduardo Levi, Luciano Cesar Scarpelli, Mauricio Lacerda Nogueira, Michele de Souza Bastos, Nathalia C. Santiago Souza, Ricardo Khouri, Sanny M. Costa Lira, Shirley Vasconcelos Komninakis, Cecile Baronti, Remi N. Charrel, Beate M. Kuemmerer, Christian Drosten, Carlos Brites, Xavier de lamballerie, Matthias Niedrig, Eduardo Martins Netto, Jan Felix Drexler
EMERGING INFECTIOUS DISEASES
(2018)
Article
Microbiology
Cecile Baronti, Geraldine Piorkowski, Franck Touret, Remi Charrel, Xavier de Lamballerie, Antoine Nougairede
GENOME ANNOUNCEMENTS
(2017)
