Article
Multidisciplinary Sciences
Joseph B. Lin, Xiaolei Shen, Charles W. Pfeifer, Fion Shiau, Andrea Santeford, Philip A. Ruzycki, Brian S. Clark, Qin Liu, Andrew J. W. Huang, Rajendra S. Apte
Summary: The study used single-cell RNA sequencing to analyze the transcriptional states of the mouse corneal epithelium in various conditions. They identified transcriptional changes in dry eye disease and proposed a classification system for markers of limbal stem cells. This study provides a framework and atlas for future research on ocular surface stem cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Ophthalmology
Xue Yang, Xin Zuo, Hao Zeng, Kai Liao, Dalian He, Bowen Wang, Jin Yuan
Summary: This study aimed to investigate the effect of gamma interferon (IFN-gamma) on corneal epithelial pyroptosis in an experimental dry eye (DE) model and explore the underlying molecular mechanisms. The results demonstrated that IFN-gamma activated the JAK2/STAT1 signaling pathway, inducing corneal epithelial pyroptosis. Additionally, oxidative stress might be downstream of JAK2/STAT1, contributing to pyroptosis.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Donghui Yu, Wenting Cai, Tianyi Shen, Yan Wu, Chengda Ren, Tingting Li, Chengyu Hu, Meijiang Zhu, Jing Yu
Summary: Dry eye disease (DED) is a common disease that affects vision and quality of life. PM2.5 has been identified as a potential risk factor for DED. This study conducted experiments on animals and cells to investigate the pathogenic effects of PM2.5 exposure on the ocular surface and the mechanisms of DED.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Oncology
Yu Han, Yu Zhang, Kelan Yuan, Yaying Wu, Xiuming Jin, Xiaodan Huang
Summary: This study investigates the mechanisms involving hyperosmolar microenvironment, glycolysis mediating metabolic reprogramming, and pyroptosis in the development of dry eye disease (DED). It is found that the hyperosmolar environment induces metabolic reprogramming in corneal epithelial cells, leading to inflammation in macrophages. Inhibition of glycolysis suppresses pyroptosis.
FRONTIERS OF MEDICINE
(2023)
Article
Ophthalmology
Xin Zuo, Hao Zeng, Bowen Wang, Xue Yang, Dalian He, Li Wang, Hong Ouyang, Jin Yuan
Summary: The study reveals that excessive oxidative stress-induced ferroptosis is involved in the pathogenesis of dry eye disease (DED). The expression of AKR1C1 is regulated by NRF2 and plays a protective role in reducing ferroptosis-induced cell damage and inflammation in corneal epithelial cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Xuan Liu, Zedu Cui, Xi Chen, Yan Li, Jin Qiu, Yuke Huang, Xiao Wang, Shuilian Chen, Qian Luo, Pei Chen, Jing Zhuang, Keming Yu
Summary: This study reveals a novel mechanism by which UCNV induces bilateral ferroptosis through the VIP/Hif1a/TfR1 pathway in lacrimal glands, providing a new therapeutic target for DES-induced by corneal refractive surgeries.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Biology
Qian Yu, Soma Biswas, Gang Ma, Peiquan Zhao, Baojie Li, Jing Li
Summary: This study elucidates the major mechanisms governing corneal maintenance, regeneration, and aging, including the role of the Rela gene, the synthesis of Retinoic acid, and the impact of angiogenesis. Interventions targeting these pathways could effectively improve corneal regeneration and aging processes.
Article
Biochemistry & Molecular Biology
Adrian Guerrero-Moreno, Hong Liang, Nathan Moreau, Jade Luzu, Ghislaine Rabut, Stephane Melik Parsadaniantz, Antoine Labbe, Christophe Baudouin, Annabelle Reaux-Le Goazigo
Summary: The study compared corneal nerve abnormalities in patients with neuropathic corneal pain secondary to MGD or AIDE. MGD-related NCP was associated with more nerve abnormalities, while AIDE-related NCP showed increased nerve tortuosity and neuromas. IVCM can be useful in detecting corneal nerve changes in NCP patients.
Article
Immunology
Jing Zhang, Yiqin Dai, Yujing Yang, Jianjiang Xu
Summary: This study found increased pyroptosis in dry eye patients and demonstrated that calcitriol could effectively alleviate hyperosmotic stress-induced corneal epithelial cell damage by inhibiting the NLRP3-ASC-caspase-1-GSDMD pyroptosis pathway. Thus, calcitriol shows promise as a therapeutic agent for dry eye due to its multiple therapeutic targets.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Rafael Martinez-Carrasco, M. Elizabeth Fini
Summary: Epitheliopathy at the ocular surface is a defining sign of dry eye disease, and the hyperosmolarity of the tear film contributes to the pathology. The unfolded protein response (UPR) and cell death are key processes in this disease, and dynasore has shown therapeutic potential in protecting corneal epithelial cells. Dynasore can inhibit cell death caused by hyperosmotic stress, protect against endoplasmic reticulum stress, and maintain UPR activity, which suggests its potential as a treatment for dry eye epitheliopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Darine Fakih, Adrian Guerrero-Moreno, Christophe Baudouin, Annabelle Reaux-Le Goazigo, Stephane Melik Parsadaniantz
Summary: The study evaluated the effectiveness of TRPV1 blockade in alleviating ocular pain, neuroinflammation, and anxiety-like behaviors associated with severe DED. Results showed that TRPV1 antagonist reduced corneal responsiveness to various stimuli and inhibited the upregulation of genes involved in neuropathic and inflammatory pain in the TG, leading to reduced ocular pain sensation and anxiety-like behaviors in severe DED.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Ophthalmology
Yun Zhang, Jin-Miao Li, Rong Lu, Zhao Liu, Xin Chen, Cintia S. de Paiva, Stephen C. Pflugfelder, De-Quan Li
Summary: This study demonstrates that the anti-inflammatory cytokine IL-37 plays a novel role in preventing corneal epithelial barrier disruption in dry eye disease by suppressing TNF-alpha and CTSS expression.
Article
Pharmacology & Pharmacy
Anita Kirti Ghosh, Rubina Thapa, Harsh Nilesh Hariani, Michael Volyanyuk, Sean David Ogle, Karoline Anne Orloff, Samatha Ankireddy, Karen Lai, Agne Ziniauskaite, Evan Benjamin Stubbs Jr, Giedrius Kalesnykas, Jenni Johanna Hakkarainen, Kelly Ann Langert, Simon Kaja
Summary: This study demonstrated the pharmacological efficacy of xanthohumol in preclinical models for dry eye disease by promoting the transcription of phase II antioxidant enzymes. Xanthohumol-encapsulating PLGA nanoparticles showed cytoprotective effects against oxidative stress in vitro and reduced ocular surface damage in vivo, suggesting their potential as future therapeutic options for dry eye disease.
Article
Ophthalmology
Dong Won Paik, Dong Hui Lim, Tae-Young Chung
Summary: This study found that taking pregabalin during LASEK surgery can reduce postoperative pain. However, after 6 months, there were no significant differences in corneal sensitivity and nerve fiber density between the pregabalin group and the control group.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ana Lopez-Machado, Natalia Diaz-Garrido, Amanda Cano, Marta Espina, Josefa Badia, Laura Baldoma, Ana Cristina Calpena, Eliana B. Souto, Maria Luisa Garcia, Elena Sanchez-Lopez
Summary: The study developed lactoferrin-loaded liposomes coated with hyaluronic acid, which showed potential in reversing dry eye symptoms and possessing anti-inflammatory efficacy without causing ocular irritation. This innovative nanotechnological tool could offer a promising approach in the treatment of Dry Eye Disease.