Article
Cell Biology
Mark S. Filla, Kristy K. Meyer, Jennifer A. Faralli, Donna M. Peters
Summary: Studies indicate that activation of αvβ3 integrin signaling may significantly impact TM cell function and POAG pathogenesis; overexpression of CA-αvβ3 integrin leads to significant differential gene expression involving multiple signaling pathways.
Article
Ophthalmology
Avani A. Mody, J. Cameron Millar, Abbot F. Clark
Summary: Elevated intraocular pressure (IOP) in primary open-angle glaucoma (POAG) is a major risk factor for irreversible vision loss. Over-expression of ID1 and ID3 can suppress TGF beta 2-induced ocular hypertension and decreased aqueous humor (AH) outflow facility. These proteins may be potential candidates for future IOP-lowering treatments in POAG.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Cell Biology
Sze-Wan Shan, Chi-Wai Do, Thomas Chuen Lam, Hoi-Lam Li, W. Daniel Stamer, Chi-Ho To
Summary: The selective ROCK inhibitor Y39983 induces actin remodeling, decreases cell motility and transepithelial resistance in human TM cells. Proteomic analysis identified 20 significantly altered proteins after Y39983 treatment, involving pathways such as p53, integrin signaling, and cytoskeletal regulation by Rho GTPase. Downregulation of TSP1 by Y39983 and its antagonists led to reduced cell migration and increased outflow facility.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Ophthalmology
Jun Wang, Hanke Wang, Yalong Dang
Summary: Glaucoma, a leading cause of irreversible blindness, can be prevented and treated by reducing intraocular pressure. ROCK inhibitors are a new class of glaucoma drugs that have a good safety profile and exert neuroprotective effects. Netarsudil and ripasudil are commonly used ROCK inhibitors.
OPHTHALMOLOGY AND THERAPY
(2023)
Article
Ophthalmology
Clemens A. Strohmaier, Fiona S. McDonnell, Xiaowei Zhang, Daniel Wanderer, W. Daniel Stamer, Robert N. Weinreb, Alex S. Huang
Summary: This study investigates the differences in outflow facility between high-flow and low-flow segments of the conventional outflow pathway in porcine eyes. It finds that there is an uneven distribution of local outflow facility across different parts of the trabecular meshwork.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Alyssa S. Aebersold, Zhao-Hui Song
Summary: This study found that non-psychotropic cannabidiol (CBD) has a positive effect on regulating intraocular pressure. It enhances aqueous humor outflow and modifies signaling pathways in trabecular meshwork (TM) cells.
Article
Medicine, General & Internal
Juliette Buffault, Francoise Brignole-Baudouin, Elodie Reboussin, Karima Kessal, Antoine Labbe, Stephane Melik Parsadaniantz, Christophe Baudouin
Summary: Comparing the effects of latanoprost and a Rho-kinase inhibitor on TGF-beta 2-induced glaucoma-like model revealed that the ROCKi was able to reverse the TGF-beta 2-induced cytoskeletal and ECM rearrangements, while latanoprost only affected the extracellular matrix.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biology
Guorong Li, Chanyoung Lee, A. Thomas Read, Ke Wang, Jungmin Ha, Megan Kuhn, Iris Navarro, Jenny Cui, Katherine Young, Rahul Gorijavolu, Todd Sulchek, Casey Kopczynski, Sina Farsiu, John Samples, Pratap Challa, C. Ross Ethier, W. Daniel Stamer
Summary: Netarsudil reduces glucocorticoid-induced ocular hypertension by preventing or attenuating pathologies, acting directly on conventional outflow cells. These findings warrant a randomized prospective clinical study to evaluate its superiority in lowering steroid-induced ocular hypertension.
Article
Biochemistry & Molecular Biology
Shruti V. Patil, Ramesh B. Kasetti, J. Cameron Millar, Gulab S. Zode
Summary: Glaucoma is a multifactorial disease that can lead to irreversible blindness. The researchers in this study developed a new mouse model of sustained intraocular pressure (IOP) elevation using lentiviral gene transfer to express active human TGF beta 2 in the trabecular meshwork (TM). This model demonstrated TM dysfunction and increased outflow resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lu Liu, Xuejiao Yang, Jingjing Zhang, Wenlan Jiang, Tianyu Hou, Yao Zong, Haiqing Bai, Kun Yang, Xian Yang
Summary: In this study, it was found that long non-coding RNA small nucleolar RNA host gene 11 (SNHG11) regulates the Wnt/β-catenin signaling pathway in trabecular meshwork (TM) cells through the Rho/ROCK pathway and β-catenin phosphorylation, affecting cell proliferation, migration, apoptosis, and autophagy. These findings suggest that SNHG11 plays a crucial role in the pathogenesis of glaucoma and could be a potential therapeutic target.
Article
Ophthalmology
Yunsheng Qiao, Zhongmou Sun, Chen Tan, Junyi Lai, Xinghuai Sun, Junyi Chen
Summary: The study demonstrates that AAV-mediated gene therapy can effectively lower intraocular pressure by remodeling the trabecular outflow pathway in mice eyes.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ramesh B. Kasetti, Prabhavathi Maddineni, Bindu Kodati, Bhavani Nagarajan, Sam Yacoub
Summary: The study demonstrates that Astragaloside IV can reduce TGF beta 2-induced ECM deposition and ER stress by inhibiting NF-κB and activating MMPs, potentially offering therapeutic benefits in alleviating glaucomatous fibrosis and elevated intraocular pressure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Biomaterials
Maria Bikuna-Izagirre, Javier Aldazabal, Leire Extramiana, Javier Moreno-Montanes, Elena Carnero, Jacobo Paredes
Summary: The researchers developed an in vitro JCT model using a polycaprolactone nanofibrous scaffold, which could recapitulate the functional JCT morphology and study the flow of aqueous humor. The model exhibited HTM-like characteristics and allowed for investigation of the relationship between aqueous humor outflow and HTM degeneration as well as drug screening.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Cell Biology
Michael Madekurozwa, W. Daniel Stamer, Ester Reina-Torres, Joseph M. Sherwood, Darryl R. Overby
Summary: Intraocular pressure (IOP) fluctuates due to ocular pulse, which induces shear stress in Schlemm's canal (SC). The ocular pulse may modulate outflow facility by stimulating NO production. Studies showed the immediate increase in outflow facility in mice in response to the ocular pulse, with NO playing a significant role in this regulation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Medicine, Research & Experimental
Yanan Wang, Wenyan Wang, Xuejiao Yang, Wenshi Chen, Xian Yang, Xiaojing Pan, Peilong Xu, Wei Zhu, Yantao Han, Xuehong Chen
Summary: Cells expressing ITGA8 in the Schlemm's canal region exhibit more properties as Schlemm's canal endothelial cells, indicating a crucial role of ITGA8 in regulating aqueous humor outflow resistance.
Review
Biochemical Research Methods
Vijay Tejwani, Mikael R. Andersen, Jong Hyun Nam, Susan T. Sharfstein
BIOTECHNOLOGY JOURNAL
(2018)
Article
Chemistry, Multidisciplinary
Melissa Cruz-Acuna, Justin R. Halman, Kirill A. Afonin, Jon Dobson, Carlos Rinaldi
Article
Biochemistry & Molecular Biology
Enping Hong, Justin R. Halman, Ankit Shah, Edward Cedrone, Nguyen Truong, Kirill A. Afonin, Marina A. Dobrovolskaia
Article
Biochemical Research Methods
Shangzhong Li, Seong Won Cha, Kelly Heffner, Deniz Baycin Hizal, Michael A. Bowen, Raghothama Chaerkady, Robert N. Cole, Vijay Tejwani, Prashant Kaushik, Michael Henry, Paula Meleady, Susan T. Sharfstein, Michael J. Betenbaugh, Vineet Bafna, Nathan E. Lewis
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Nooshin Amini, Janet L. Paluh, Yubing Xie, Vinit Saxena, Susan T. Sharfstein
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Vandhana Muralidharan-Chari, Zachary Wurz, Francis Doyle, Matthew Henry, Andreas Diendorfer, Scott A. Tenenbaum, Nicole Borth, Edward Eveleth, Susan T. Sharfstein
Summary: The novel technology, PTSelect(TM), utilizes siRNA cloned upstream of the gene of interest to enable cell enrichment and selection. Compared to conventional methods, PTSelect(TM) demonstrates comparable stability and productivity, and allows for easy monitoring of productivity changes due to clonal drift.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Vijay Tejwani, Minal Chaudhari, Toyaj Rai, Susan T. Sharfstein
Summary: Mammalian cell line development is a multistep process involving single-cell cloning, monoclonality assurance, productivity and stability screening, which can be labor, time and resource intensive when performed manually. Introduction of automation and miniaturization has reduced manual labor, shortened timelines, and decreased resources required for developing manufacturing cell lines.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Biotechnology & Applied Microbiology
Bryan E. Thacker, Kristen J. Thorne, Colin Cartwright, Jeeyoung Park, Kimberly Glass, Annie Chea, Benjamin P. Kellman, Nathan E. Lewis, Zhenping Wang, Anna Di Nardo, Susan T. Sharfstein, Walter Jeske, Jeanine Walenga, John Hogwood, Elaine Gray, Barbara Mulloy, Jeffrey D. Esko, Charles A. Glass
Summary: Heparin, an essential anticoagulant, is currently sourced from vulnerable animal populations. This study demonstrates the feasibility of producing high anticoagulant potency heparin sulfate through genome engineering in cell culture, providing a potential alternative to animal sources.
METABOLIC ENGINEERING
(2022)
Article
Engineering, Biomedical
Pujhitha Ramesh, Nicholas Moskwa, Zachary Hanchon, Adam Koplas, Deirdre A. Nelson, Kristen L. Mills, James Castracane, Melinda Larsen, Susan T. Sharfstein, Yubing Xie
Summary: In this study, cryoelectrospinning was used to fabricate scaffolds that mimic the structure and function of soft-tissue ECM. The researchers found that these scaffolds supported cell survival and exhibited potential for use in regenerative medicine applications.
Review
Medicine, Research & Experimental
Susan T. Sharfstein
Summary: Bio-hybrid devices, which combine electronic and photonic components with cells, tissues, and organs, have the potential to advance our understanding of biology, physiology, and pathologies, as well as treat a wide range of conditions and diseases. This review discusses the devices, materials, and technologies that make bio-hybrid devices possible, and provides examples of their application at various biological scales. It also describes the outcomes of a National Science Foundation (NSF)-funded workshop that envisions the potential applications of these technologies in improving health outcomes and quality of life.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Luke A. Beardslee, Justin R. Halman, Andrea M. Unser, Yubing Xie, John Danias, Magnus Bergkvist, Susan T. Sharfstein, Karen Y. Torrejon
Summary: Glaucoma, a leading cause of irreversible blindness, is characterized by increased intraocular pressure (IOP) and optic nerve damage. Tissue-engineered replacement of damaged human trabecular meshwork (HTM) cells using polycaprolactone (PCL) scaffolds is a potential future treatment. This study demonstrated the feasibility of using gelatin-coated, micropatterned, ultrathin, porous PCL scaffolds to support proper HTM cell growth, cytoskeletal organization, HTM-marker expression, and extracellular matrix (ECM) deposition for tissue-engineered implantable ocular outflow tissue.
BIOENGINEERING-BASEL
(2023)
Article
Biophysics
Fernando Pesantez Torres, Natalya Tokranova, Eleanor Amodeo, Taylor Bertucci, Thomas R. Kiehl, Yubing Xie, Nathaniel C. Cady, Susan T. Sharfstein
Summary: The biocompatibility of materials used in electronic devices is crucial for the development of implantable devices and biomanufacturing. This study evaluated the biocompatibility of silicon chips coated with metal thin films and found that these materials are biocompatible and can support neural differentiation.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biotechnology & Applied Microbiology
Matthew Jorgensen, Pujhitha Ramesh, Miriam Toro, Emily Evans, Nicholas Moskwa, Xulang Zhang, Susan T. Sharfstein, Melinda Larsen, Yubing Xie
Summary: Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential. This study demonstrated a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial-mesenchymal cell interaction for 3D salivary gland cell organization. The findings suggest that hydrogel microtubes are promising for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future applications in regenerative medicine.
BIOENGINEERING-BASEL
(2022)
Meeting Abstract
Ophthalmology
Karen Y. Torrejon, Cula N. Dautriche, Andrea Unser, Mathew Kerr, Feryan Ahmed, Abbey Van Alstyne-Ponce, Dan Stamer, Susan T. Sharfstein, Yubing Xie, John Danias
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Article
Biotechnology & Applied Microbiology
Hussain Dahodwala, Prashant Kaushik, Vijay Tejwani, Chih-Chung Kuo, Patrice Menard, Michael Henry, Bjorn G. Voldborg, Nathan E. Lewis, Paula Meleady, Susan T. Sharfstein
CURRENT RESEARCH IN BIOTECHNOLOGY
(2019)