Article
Immunology
Priyasha Mishra, Sanjay Ch, Seok Jong Hong, Swati Biswas, Sanhita Roy
Summary: This study investigated the role of host defense peptide S100A12 against Pseudomonas and observed its antimicrobial activity through immunohistochemistry and colony forming unit experiments. In a mouse model of Pseudomonas keratitis, S100A12 reduced corneal opacity and bacterial load. Further transcriptome analysis revealed that S100A12 inhibits multiple important pathways in Pseudomonas.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Sophie A. Howard, R. Christopher D. Furniss, Dora Bonini, Himani Amin, Patricia Paracuellos, David Zlotkin, Tiago R. D. Costa, Asaf Levy, Despoina A. I. Mavridou, Alain Filloux
Summary: The T6SS is a bacterial nanoscale weapon that delivers toxins into prey and plays a key role in the arms race between organisms. By using Hcp as bait, researchers identified uncharacterized toxins and investigated the role of Hcp in the T6SS mechanism.
Article
Multidisciplinary Sciences
Patricia Bernal, R. Christopher D. Furniss, Selina Fecht, Rhoda C. Y. Leung, Livia Spiga, Despoina A. Mavridou, Alain Filloux
Summary: The T6SS is a nanomachine primarily involved in interbacterial competition, with TssA being a key component for its assembly. Researchers have found unexpected diversity in TssA proteins, with a short form and a long form existing. The discovery of structural components that interact with short TssA proteins sheds light on the mechanisms for T6SS assembly and functioning.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Environmental
Sebastien R. David, Agathe Jaouen, Dris Ihiawakrim, Valerie A. Geoffroy
Summary: This study investigated the role of siderophore-producing Pseudomonas in the biodeterioration of asbestos cement, finding that various pyoverdines were efficient in extracting iron from the waste. The research suggests a potential bioremediation process for avoiding disposal of such waste in landfills.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Filippo Dell'Anno, Giovanni Andrea Vitale, Carmine Buonocore, Laura Vitale, Fortunato Palma Esposito, Daniela Coppola, Gerardo Della Sala, Pietro Tedesco, Donatella de Pascale
Summary: This review article summarizes the biosynthesis, pathogenic mechanisms, and therapeutic applications of PVDs, highlighting their importance in microbial survival. It also evaluates the potential of PVDs as therapeutic targets and provides a review of the latest methods and techniques for drug discovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Zhen Tao, Haoda Ye, Chaozheng Zhang, Suming Zhou, Kequan Wang, Mingming Zhang, Jianjun Xie, Hetron Mweemba Munang'andu, Cheng Xu, Pengcheng Wang, Xiaojun Yan
Summary: In this study, a novel two-component system PvgAS has been identified in P. plecoglossicida, which regulates the expression of T6SS-1 and plays a crucial role in bacterial pathogenicity. This finding provides important insights into the understanding of the pathogenic mechanisms of P. plecoglossicida and potential therapeutic interventions against this bacterium.
Article
Biochemistry & Molecular Biology
Jiuxin Qu, Zhao Cai, Xiangke Duan, Han Zhang, Hang Cheng, Shuhong Han, Kaiwei Yu, Zhaofang Jiang, Yingdan Zhang, Yang Liu, Fang Bai, Yingxia Liu, Lei Liu, Liang Yang
Summary: Our study suggests that SARS-CoV-2 infection can lead to genetic adaptation of bacterial pathogens, promoting chronic infections. The adapted bacteria exhibit characteristics such as excessive biofilm formation and reduced virulence, enabling them to evade immune clearance. This highlights the importance of understanding the lung microenvironment post-virus infections and superinfection mechanism in COVID-19 patients.
CELL AND BIOSCIENCE
(2022)
Article
Microbiology
Chih-Feng Wu, Alexandra J. Weisberg, Edward W. Davis, Lin Chou, Surtaz Khan, Erh-Min Lai, Chih-Horng Kuo, Jeff H. Chang
Summary: The T6SS is used by many Gram-negative bacteria for interbacterial competition, with diverse strains of agrobacteria showing different patterns of T6SS expression and enrichment in specific sets of T6SS loci. Diversification is driven by the reshuffling of entire loci and fragments, but constrained by the maintenance of specific gene subtypes.
Article
Biotechnology & Applied Microbiology
Jun Li, Linlin Xie, Shulan Qian, Yuhang Tang, Mingjie Shen, Shanshan Li, Jie Wang, Lie Xiong, Jie Lu, Weihong Zhong
Summary: The T6SS in strain JY-Q mediated resistance to oxidative stress and promoted bacterial fitness via TseN, a dual-functional effector with antimicrobial and anti-ROS activities. TseN showed potential antagonism against ROS to fine tune the heavy traffic of nicotine metabolism in strain JY-Q. This study revealed a working model between T6SS and nicotine metabolism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
Summary: YbeY is a highly conserved RNase in bacteria that plays essential roles in rRNA processing, small RNA regulation, and bacterial responses to environmental stresses. Mutation of the ybeY gene in Pseudomonas aeruginosa leads to dysregulation of secretion system gene expression and biofilm formation, indicating the pleiotropic functions of YbeY in bacterial regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Cell Biology
Wenbo He, Ke Wu, Zhenlin Ouyang, Yixin Bai, Wen Luo, Di Wu, Hao An, Yucheng Guo, Min Jiao, Qian Qin, Jiaxin Zhang, Yi Wu, Junjun She, Peter M. Hwang, Fang Zheng, Li Zhu, Yurong Wen
Summary: The Type VI secretion system is commonly used by Gram-negative bacteria to inject toxic effectors into nearby cells. The core components of the system, Hcp, VgrG, or PAAR, allow for the loading of various effectors onto the delivery tube. Through cryo-EM and crystal structure analyses, this study provides insights into the assembly, loading, and firing of the T6SS nanomachine, contributing to bacterial interspecies competition and host interactions.
Article
Microbiology
Derek C. K. Chan, Lori L. L. Burrows
Summary: Iron is essential for bacterial growth but its poor solubility hampers this process. Bacteria produce siderophores to bind iron and overcome this limitation. Pseudomonas aeruginosa, an opportunistic pathogen, has multiple transporters that recognize its own siderophores as well as those produced by other bacteria or fungi, giving it a competitive advantage.
Article
Infectious Diseases
Kei Ikarashi, Ryo Kutsuna, Junko Tomida, Yoshiaki Kawamura, Yuji Morita
Summary: The study found that MexXY overexpression and deficiency in multidrug-resistant Pseudomonas aeruginosa can impact pyoverdine production, with MexXY deficiency leading to increased pyoverdine production. MexXY-deficient mutants may be more susceptible to iron deficiency, while high-risk clones are usually MexXY overproducers but defective pyoverdine producers.
Review
Microbiology
Xiaobing Yang, Hai Liu, Yanxiong Zhang, Xihui Shen
Summary: The type VI secretion system (T6SS) in gram-negative bacteria has multiple functions, including the newly discovered function of metal ion transport. The review provides a summary of identified T6SS functions and discusses the features and regulation of metal ion transport mediated by T6SS.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Youzhou Liu, Chen Dai, Yaqiu Zhou, Junqing Qiao, Bao Tang, Wenjie Yu, Rongshen Zhang, Yongfeng Liu, Shi-En Lu
Summary: The study showed that PVDs are crucial for the broad-spectrum antibacterial activities of strain YL-1 against both Gram-positive and Gram-negative bacteria under low-iron conditions. The findings also emphasize the impact of exogenous iron on PVD production and the significance of this bacterial product in bacterial interactions.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Ewelina P. Dutkiewicz, Chun-Han Su, Han-Jung Lee, Cheng-Chih Hsu, Yu-Liang Yang
Summary: Imaging mass spectrometry (IMS) is a powerful technique for analyzing various molecular species with high spatial resolution and low detection limits. Functionalized TiO2 nanowire substrate-based surface-assisted laser desorption/ionization (SALDI) is an effective complementary technique to MALDI-MS for detecting low-molecular-weight species in plant tissues.
Article
Multidisciplinary Sciences
Yi-Jen Sun, Fan Bai, An-Chi Luo, Xiang-Yu Zhuang, Tsai-Shun Lin, Yu-Cheng Sung, Yu-Ling Shih, Chien-Jung Lo
Summary: The article presents a method for high-resolution analysis of active and inert zones of cell wall growth during bacterial elongation, and formulates a mathematical model to predict the partitioning of cell wall-anchored proteins following cell division.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Yang-Ming Chen, Aniket Limaye, Hui-Wen Chang, Je-Ruei Liu
Summary: This study screened 60 LAB strains for their antiviral activity against PEDV and identified two strains with prophylactic effects on Vero cells.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Biochemistry & Molecular Biology
Pei-Yu Hsieh, Fan-Chun Meng, Chih-Wei Guo, Kung-Hsiang Hu, Yu-Ling Shih, Wei-Chieh Cheng
Summary: Fluorescent probes play a crucial role in imaging bacterial PGN, especially for labeling key PGN-synthesizing enzymes like TGases. This study successfully synthesized the first imaging probe for labeling TGase, providing a new method for monitoring bacterial growth and division cycles, as well as studying cell wall growth when conventional probes are not applicable.
Article
Microbiology
Po-Wen Tu, Jie-Siang Chiu, Chih Lin, Chih-Cheng Chien, Feng-Chia Hsieh, Ming-Che Shih, Yu-Liang Yang
Summary: This study demonstrated that Photorhabdus akhurstii sp. nov. 0813-124 has the potential to control the devastating fungus Colletotrichum gloeosporioides in strawberries. The bioactive ingredients, glidobactin A and cepafungin I, were identified and their expression in different phases was analyzed. These findings suggest that P. akhurstii sp. nov. 0813-124 could be used as a biocontrol agent or as part of a natural product repertoire for combating C. gloeosporioides.
Article
Biology
Ching-Chih Lin, Sin Yong Hoo, Li-Ting Ma, Chih Lin, Kai-Fa Huang, Ying-Ning Ho, Chi-Hui Sun, Han-Jung Lee, Pi-Yu Chen, Lin-Jie Shu, Bo-Wei Wang, Wei-Chen Hsu, Tzu-Ping Ko, Yu-Liang Yang
Summary: In a multi-omics analysis, bacterial polyynes were found to act as antifungal agents by inhibiting the Candida albicans polyyne resistance gene ERG10, providing a potential target for developing antifungal agents.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chun-Han Su, Yu-Chieh Cheng, Yu-Chia Chang, Ting-Hsuan Kung, Yu-Li Chen, Kuei-Hung Lai, Hsi-Lung Hsieh, Chun-Yu Chen, Tsong-Long Hwang, Yu-Liang Yang
Summary: This study examined the antiproliferative activity of different extracts of Tetradium ruticarpum (TR) fruit and highlighted potentially antiproliferative compounds using an untargeted LC-MS/MS-based multi-informative molecular networking strategy. The EtOAc fraction F2-3 exhibited the most potent antiproliferative activity against human cell lines. Through computational tool-aided structure prediction and integration of various data, 11 indole alkaloids and 47 types of quinolone alkaloids were successfully annotated and visualized into three targeted bioactive molecular families. Several previously unreported quinolone alkaloids in TR fruit were discovered. Four indole alkaloids and five types of quinolone alkaloids were identified as potentially antiproliferative compounds in the EtOAc fraction F2-3. Evodiamine was confirmed as a major antiproliferative compound, and its structural analogues found in the molecular network showed promise as antitumor agents. This study exemplifies the application of an LC-MS/MS-based multi-informative molecular networking strategy in the discovery and annotation of bioactive compounds from complex mixtures of potential functional food ingredients.
Article
Entomology
Yi-Hsuan Li, Zih-Ting Chang, Ming-Ren Yen, Yu-Feng Huang, Tzu-Han Chen, Ju-Chun Chang, Ming-Cheng Wu, Yu-Liang Yang, Yue-Wen Chen, Yu-Shin Nai
Summary: This study investigated the gene expression profile of Nosema ceranae, a fungal parasite of honey bees, at different time points post-infection. The results revealed that the gene expression of N. ceranae during infection is closely related to gene transcription, protein synthesis, and structural proteins. The findings have important implications for the control of nosemosis in apiculture.
Article
Plant Sciences
Ru-Ying Feng, Yi-Hsuan Chen, Chih Lin, Chia-Hsin Tsai, Yu-Liang Yang, Ying-Lien Chen
Summary: This study aimed to determine whether surfactin is the main compound active against Streptomyces scabies in potato common scab. The results showed that the srf gene cluster of Bacillus amyloliquefaciens Ba01 is responsible for the secretion of surfactin and inhibition of S. scabies. Knocking out the srf gene cluster significantly decreased the swarming ability, biofilm formation, enzyme activity, and inhibitory effect of Ba01 on S. scabies.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Integrative & Complementary Medicine
Yi-Hsiang Wang, Aniket Limaye, Je-Ruei Liu, Tai-Na Wu
Summary: Influenza, an infectious disease caused by influenza viruses, can lead to mild symptoms or severe complications and death. Annual influenza vaccines are the primary intervention, but their effectiveness is limited, especially in vulnerable populations. Therefore, alternative methods to curb influenza incidence, particularly for at-risk individuals, are needed.
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
(2023)
Article
Multidisciplinary Sciences
Hong Thuy Vy Nguyen, Xiaorui Chen, Claudia Parada, An-Chi Luo, Orion Shih, U-Ser Jeng, Chia-Ying Huang, Yu-Ling Shih, Che Ma
Summary: The FtsB-FtsL-FtsQ complex plays a crucial role in regulating cell wall synthesis during bacterial cell division by mediating the synthesis of the cell-wall peptidoglycan. The full-length structure of the heterotrimeric FtsBLQ complex is reported, showing a tilted V-shaped conformation. This structure may facilitate interactions with other divisome proteins in an allosteric manner, leading to a proposed mechanism for the regulation of peptidoglycan synthases by the FtsBLQ complex.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Wachiraporn Toopaang, Kullyanee Panyawicha, Chettida Srisuksam, Wei-Chen Hsu, Ching-Chih Lin, Morakot Tanticharoen, Yu-Liang Yang, Alongkorn Amnuaykanjanasin
Summary: This study investigated the metabolite profiles of pks14 and pks15 gene overexpression strains in B. bassiana and compared them to their knockout strains using LC-MS/MS with GNPS. The results showed that pks14 and pks15 clusters interacted with biosynthetic clusters encoding insect-virulent metabolites and upregulated certain secondary metabolites. This suggests that pks14 and pks15 may be directly or indirectly associated with key pathways in insect pathogenesis of B. bassiana.
Article
Biotechnology & Applied Microbiology
Hieu Tran Nguyen Minh, Tien-Fen Kuo, Wen-Yu Lin, Tzu-Chia Peng, Greta Yang, Chih-Yu Lin, Ting-Hsiang Chang, Yu-Liang Yang, Cheng-Hsun Ho, Bor-Rung Ou, Chu-Wen Yang, Yu-Chuan Liang, Wen-Chin Yang
Summary: This study investigates the function and mechanism of a phytogenic formulation, EUBIO-BPSG, in laying hens and finds that it improves health and egg production while increasing beneficial bacteria and decreasing pathogens, as well as reducing antibiotic resistance and resistance genes.
BIOENGINEERING-BASEL
(2023)
Review
Biochemistry & Molecular Biology
Wachiraporn Toopaang, Warapon Bunnak, Chettida Srisuksam, Wilawan Wattananukit, Morakot Tanticharoen, Yu-Liang Yang, Alongkorn Amnuaykanjanasin
Summary: This review discusses the role and function of polyketides in entomopathogenic microorganisms, and their involvement in insect pathogenesis and host-fungus interaction. Understanding the mechanisms of polyketides can facilitate the discovery and development of novel bio-insecticides.
NATURAL PRODUCT REPORTS
(2022)