Article
Multidisciplinary Sciences
Natalie Y. Chen, Paul H. Kim, Yiping Tu, Ye Yang, Patrick J. Heizer, Stephen G. Young, Loren G. Fong
Summary: The expression level of LAP2I3 is negatively correlated with the frequency of nuclear membrane ruptures in cells deficient in nuclear lamins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Rhiannon M. Sears, Kyle J. Roux
Summary: Mutations in LMNA and BANF1 genes can cause progeria, leading to accelerated aging. The proteins produced by these genes are involved in nuclear envelope rupture and repair processes. A-type lamins are recruited to the rupture sites in a BAF-dependent manner. Progeria-associated mutations inhibit the recruitment of A-type lamins to nuclear ruptures.
Article
Multidisciplinary Sciences
Veer I. P. Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aize, Maud Bongaerts, Fanny Delille, Lorena Kolar-Znika, Vittore F. Scolari, Sebastian Hoffmann, Edward J. Banigan, Leonid A. Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon
Summary: This study introduces a method to manipulate a genomic locus using controlled magnetic forces inside the nucleus of a living human cell. The results show that chromatin exhibits viscoelastic displacements in response to near-piconewton forces, challenging the view that interphase chromatin is a gel-like material.
Article
Cell Biology
Jinmei Cheng, Edward S. Allgeyer, Jennifer H. Richens, Edo Dzafic, Amandine Palandri, Bohdan Lewkow, George Sirinakis, Daniel St Johnston
Summary: The line scanning microscope provides optical sectioning for single-molecule localization microscopy (SMLM) in tissues, achieving high-resolution imaging of nuclear pores and F-actin. The study reveals nonrandom distribution of nuclear pores in most Drosophila tissues, in contrast to what is observed in cultured cells. Lamin Dm(0) shows complementary localization to the nuclear pores, while ectopic expression of tissue-specific Lamin C leads to more random distribution of nuclear pores.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Allon Wagner, Chao Wang, Johannes Fessler, David DeTomaso, Julian Avila-Pacheco, James Kaminski, Sarah Zaghouani, Elena Christian, Pratiksha Thakore, Brandon Schellhaass, Elliot Akama-Garren, Kerry Pierce, Vasundhara Singh, Noga Ron-Harel, Vivian Paraskevi Douglas, Lloyd Bod, Alexandra Schnell, Daniel Puleston, Raymond A. Sobel, Marcia Haigis, Erika L. Pearce, Manoocher Soleimani, Clary Clish, Aviv Regev, Vijay K. Kuchroo, Nir Yosef
Summary: The study utilized the Compass algorithm in combination with single-cell RNA sequencing and flux balance analysis to reveal the link between metabolic states and functional variability of Th17 cells, validating the importance of metabolic switch for Th17 pathogenicity.
Article
Oncology
Julien L. P. Morival, Halida P. Widyastuti, Cecilia H. H. Nguyen, Michael Zaragoza, Timothy L. Downing
Summary: The study found family-specific DMRs in fibroblasts caused by LMNA mutations associated with dilated cardiomyopathy (DCM), linked to genes related to laminopathy phenotypes. Inter-family epimutation hotspots near differentially expressed genes found outside LADs redistributed in LMNA-related DCM were identified. Comparison of DMRs in fibroblasts and iPSCs revealed persistent epimutations across both cell types.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Hsiang-Hao Chuang, Ming-Shyan Huang, Yen-Yi Zhen, Cheng-Hao Chuang, Ying-Ray Lee, Michael Hsiao, Chih-Jen Yang
Summary: FAK inhibition not only elicits DNA-damage signaling but also downregulates EZH2 expression. Active FAK signaling corresponds with the activation of the EZH2-mediated signaling cascade in lung-cancer-cells-derived tumor tissues. Ectopic EZH2 expression attenuates FAK-inhibition-induced cellular senescence in lung cancer cells.
Article
Biochemistry & Molecular Biology
Ana de Lope-Planelles, Raquel Gonzalez-Novo, Elena Madrazo, Gracia Peralta-Carrero, Maria Pilar Cruz Rodriguez, Hector Zamora-Carreras, Veronica Torrano, Horacio Lopez-Menendez, Pedro Roda-Navarro, Francisco Monroy, Javier Redondo-Munoz
Summary: Nuclear deformability is crucial for cell migration and can lead to changes in DNA damage and cell behavior. This study found that persistent migration can cause permanent modifications in migratory-altered cells, resulting in changes in lamin B1 localization, cell morphology, and transcription. Migratory-altered cells also showed functional differences in DNA repair, cell response to chemotherapy, and in vivo cell migration experiments. These observations suggest that mechanical compression during persistent cell migration plays a role in stable nuclear and genomic alterations, which may be important for understanding genetic instability and cellular heterogeneity in aging diseases and cancer.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Food Science & Technology
Jinsook Ahn, Tae-Gyun Woo, So-mi Kang, Inseong Jo, Jae-Sung Woo, Bum-Joon Park, Nam-Chul Ha
Summary: Nuclear lamin A/C is crucial for maintaining nuclear envelope structure, with Hutchinson-Gilford progeria syndrome (HGPS) being caused by gene mutation. Natural compounds like morin have been found to disrupt progerin-mediated nuclear deformation, offering potential benefits for HGPS patients and aging individuals.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Chemistry, Multidisciplinary
Kai Wang, Kun Man, Jiafeng Liu, Brian Meckes, Yong Yang
Summary: Regulating cell behavior using nanotopography is widely used, but the complex effects of physical and biochemical cues hinder our understanding, limiting its biomedical applications. To analyze the individual roles, substrates with different physical nanotopography but identical biochemical nanopatterns and vice versa were fabricated. The results showed physical topographies dominantly control cell spreading, YAP localization, proliferation, and differentiation, while biochemical nanopatterns affect hMSC elongation, YAP intracellular localization, and LAMAC expression. Physical nanogratings induce nanoscale curvature of nuclei, attenuating osteogenic differentiation of hMSCs. Fine-tuning cell behavior is possible by altering the presentation of biochemical cues on substrate surfaces.
Article
Biochemistry & Molecular Biology
Rebecca Hetz, Carlo Magaway, Jaylene Everett, Ling Li, Belinda B. Willard, Hudson H. Freeze, Ping He
Summary: NGLY1 deficiency is a rare genetic disorder characterized by a variety of clinical features. Recent studies have identified several possible molecular mechanisms of NGLY1 deficiency. The studies also revealed abnormal accumulation of endoplasmic reticulum-associated degradation substrates and elevated levels of endogenous proteins in NGLY1-deficient cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Laura Gil, Erika Chi-Ahumada, Sandra A. Nino, Gabriela Capdeville, Areli M. Mendez-Torres, Carmen Guerrero, Ana B. Rebolledo, Isabel M. Olazabal, Maria E. Jimenez-Capdeville
Summary: This study found nuclear alterations in granular cells in autopsied human brains, which may be associated with early Alzheimer's disease. Senile and early AD samples showed different chromatin features, indicating changes in chromatin during disease progression. In the intermediate and late stages of AD, there was a gradual disappearance of phosphorylated Tau forms in the nucleus, increased chromatin disorganization, and increased nuclear autophagy, supporting a model of biphasic neurogenesis in Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Yi Zhou, Liang Hu, Wenqing Tang, Dongping Li, Lijie Ma, Hongchun Liu, Shuncai Zhang, Xiaojie Zhang, Ling Dong, Xizhong Shen, She Chen, Ruyi Xue, Si Zhang
Summary: This study revealed that upregulated NOD2 expression in hepatocellular carcinoma (HCC) patients is associated with poor prognosis, and NOD2 plays a role in translating gut dysbiosis signaling into hepatocarcinogenesis through activation of inflammatory response and nuclear autophagy pathways. Hepatic NOD2 may be a promising therapeutic target against HCC.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Article
Endocrinology & Metabolism
Hao Zhang, Renkai Wang, Guangchao Wang, Bo Zhang, Chao Wang, Di Li, Chen Ding, Qiang Wei, Zhenyu Fan, Hao Tang, Fang Ji
Summary: B cells in the bone marrow microenvironment play a crucial role in fracture healing by inhibiting excessive bone regeneration through the production of osteoblast inhibitors. Their numbers fluctuate during different stages of fracture healing, impacting the overall bone regrowth process.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Immunology
David E. Phelan, Masahiko Shigemura, Sarah Aldhafiri, Catarina Mota, Thomas J. Hall, Jacob I. Sznajder, Evelyn P. Murphy, Daniel Crean, Eoin P. Cummins
Summary: The NR4A family members NR4A2 and NR4A3 have distinct and common roles in transcriptional regulation in monocytes, with NR4A2 associated with antigen presentation and MHC genes, and NR4A3 linked to viral response pathways. Functional studies suggest preferential roles for NR4A2 in antigen processing and common roles for both NR4A2 and NR4A3 in cell migration. This study provides new insights into the mechanisms of action of NR4A2 and NR4A3 in monocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Correction
Computer Science, Interdisciplinary Applications
Michiel Van Dyck, Rajiv K. Mishra, Francisco Pestana, Peter Verstraelen, Hilde Lavreysen, Juan D. Pita-Almenar, Nachiket D. Kashikar, Winnok H. De Vos
Article
Biochemistry & Molecular Biology
Gaelle Houthaeve, Gerardo Garcia-Diaz Barriga, Stephan Stremersch, Herlinde De Keersmaecker, Juan Fraire, Jo Vandesompele, Pieter Mestdagh, Stefaan De Smedt, Kevin Braeckmans, Winnok H. De Vos
Summary: The study investigates the effects of VNB photoporation on cell homeostasis, finding that A-type lamins are upregulated after photoporation and contribute to the recovery of the nucleus. Selective depletion of A-type lamins reduces cell viability, while stimulation of LMNA transcription increases the survival rate of successfully transfected cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Physics, Multidisciplinary
Marine Luciano, Shi-Lei Xue, Winnok H. De Vos, Lorena Redondo-Morata, Mathieu Surin, Frank Lafont, Edouard Hannezo, Sylvain Gabriele
Summary: Experiments on cell monolayers on corrugated hydrogels reveal the effects of local curvature on the shape of cells and nuclei. A vertex model lends support to the idea that the modulation of tissue thickness may enable curvature sensing.
Article
Chemistry, Multidisciplinary
Fengzhen Cui, Qingfei Pan, Siyi Wang, Faming Zhao, Runxin Wang, Tingting Zhang, Yaying Song, Jun He, Haolin Zhang, Qiang Weng, Yang Jin, Wei Xia, Yuanyuan Li, Guo-Yuan Yang, Winnok H. De Vos, Jean-Pierre Timmermans, Shunqing Xu, Yaohui Tang, Xia Sheng
Summary: The study reveals the neurodevelopmental toxicity of benzophenones during pregnancy, which impairs hippocampal development and causes cognitive dysfunction in offspring mice by activating specific signaling pathways that lead to inflammation and cell apoptosis.
Review
Nanoscience & Nanotechnology
Gaelle Houthaeve, Stefaan C. De Smedt, Kevin Braeckmans, Winnok H. De Vos
Summary: Delivery of nanomaterials into cells by disrupting the plasma membrane (PM) can have various effects on cell viability and physiology, including permanent phenotypic alterations. Understanding this cellular response is crucial for optimizing nano-delivery conditions.
Review
Oncology
Sofia Zanotti, Bieke Decaesteker, Suzanne Vanhauwaert, Bram De Wilde, Winnok H. De Vos, Frank Speleman
Summary: Neuroblastoma, a common tumor in children under five, has poor treatment outcomes for high-risk cases and causes long-term side effects. Cellular senescence has been considered a barrier against tumorigenesis, but recent research suggests a more complex and reversible nature for this process, which may impact treatment strategies. Neuroblastoma cells in a senescent state may escape treatment and promote relapse, but senescence could also provide opportunities for new therapies.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Auchi Inalegwu, Bart Cuypers, Jurgen Claesen, Ann Janssen, Amelie Coolkens, Sarah Baatout, Kris Laukens, Winnok H. De Vos, Roel Quintens
Summary: Radiotherapy is the standard treatment for breast cancer, but surviving radioresistant cells can lead to relapse. Understanding the molecular mechanisms of radiation resistance can improve the treatment of radioresistant tumors. Emulating radiation therapy at the cellular level, researchers exposed breast cancer cells to daily radiation doses and found that radioresistant cells showed altered gene expression and similarities with resistance profiles seen in both radiation and endocrine therapy, suggesting potential combination therapies.
MOLECULAR ONCOLOGY
(2022)
Article
Clinical Neurology
Elise Van Breedam, Aleksandra Nijak, Tamariche Buyle-Huybrecht, Julia Di Stefano, Marlies Boeren, Jonas Govaerts, Alessandra Quarta, Tine Swartenbroekx, Eva Z. Jacobs, Bjorn Menten, Rik Gijsbers, Peter Delputte, Maaike Alaerts, Behrouz Hassannia, Bart Loeys, Zwi Berneman, Jean-Pierre Timmermans, Philippe G. Jorens, Tom Vanden Berghe, Erik Fransen, An Wouters, Winnok H. De Vos, Peter Ponsaerts
Summary: The study presents a method using human iPSC-derived neurospheroid model for monitoring neural viability under ischemic conditions. The results show that 1-week-old neurospheroids can partially recover neural cells post-OGD, while 4-week-old neurospheroids cannot, highlighting the importance of more mature neurospheroids for better recapitulation of in vivo conditions.
Article
Biology
Aleksandra Nijak, Eline Simons, Bert Vandendriessche, Dieter Van de Sande, Erik Fransen, Ewa Sieliwonczyk, Ilse Van Gucht, Emeline Van Craenenbroeck, Johan Saenen, Hein Heidbuchel, Peter Ponsaerts, Alain J. Labro, Dirk Snyders, Winnok De Vos, Dorien Schepers, Maaike Alaerts, Bart L. Loeys
Summary: Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) are a valuable tool for cardiovascular research. This study evaluated two protocols for differentiating iPSCs into cardiomyocytes and found that both protocols resulted in a high percentage of differentiated cells with typical cardiac characteristics. One protocol showed better accessibility for experiments and expressed more cardiac-specific markers.
Article
Geriatrics & Gerontology
Nadine Saul, Ineke Dhondt, Mikko Kuokkanen, Markus Perola, Clara Verschuuren, Brecht Wouters, Henrik von Chrzanowski, Winnok H. De Vos, Liesbet Temmerman, Walter Luyten, Aleksandra Zecic, Tim Loier, Christian Schmitz-Linneweber, Bart P. Braeckman
Summary: In this study, a GWAS was conducted to identify potential genetic factors associated with healthy ageing. Candidate genes were then verified using a model organism, C. elegans, and several genes were found to potentially play a role in maintaining health in the elderly.
Article
Biochemical Research Methods
Freya Molenberghs, Marlies Verschuuren, Michael Barbier, Johannes J. Bogers, Nathalie Cools, Peter Delputte, Mario Schelhaas, Winnok H. De Vos
Summary: In this study, high-content microscopy was used to investigate the early phase of HPV infection. Differences in infection between cell lines were measured using a fluorescent signal, which directly correlated with host cell proliferation rate. Nuclear organization and promyelocytic leukemia protein body content were altered by HPV infection, positioning these events at the early stage of HPV infection, upstream of viral replication.
Article
Clinical Neurology
Monica van den Berg, Mohit H. Adhikari, Marlies Verschuuren, Isabel Pintelon, Tamara Vasilkovska, Johan Van Audekerke, Stephan Missault, Loran Heymans, Peter Ponsaerts, Winnok H. De Vos, Annemie Van der Linden, Georgios A. Keliris, Marleen Verhoye
Summary: This study investigates how whole-brain networks are influenced in pre- and early-plaque stages of AD, revealing decreased activity of the basal forebrain and the default mode-like network in pre-plaque stage, and activation of compensatory mechanisms in the early-plaque stage. The findings suggest that modulating astrogliosis in the basal forebrain could be a promising therapeutic strategy to restore brain network function in AD.
ALZHEIMERS RESEARCH & THERAPY
(2022)
Article
Pathology
Cedric Peleman, Winnok H. De Vos, Isabel Pintelon, Ann Driessen, Annelies Van Eyck, Christophe Van Steenkiste, Luisa Vonghia, Joris De Man, Benedicte Y. De Winter, Tom Vanden Berghe, Sven M. Francque, Wilhelmus J. Kwanten
Summary: A new image analysis method was used to quantify the differences between liver lobule zones in NAFLD. The results showed that this method accurately measured the distribution of hypoxia markers in the steatotic liver and overcame the impact of steatosis on pathologists' interpretation.
Article
Biochemistry & Molecular Biology
Ilia Goemaere, Deep Punj, Aranit Harizaj, Jessica Woolston, Sofie Thys, Karen Sterck, Stefaan C. De Smedt, Winnok H. De Vos, Kevin Braeckmans
Summary: Photoporation is a gentle and efficient method for transfecting cells, and response surface methodology (RSM) proves to be a valuable approach for optimizing the photoporation procedure. By varying PDNP size, concentration, and laser fluence, the RSM designs successfully identified an optimal delivery yield and revealed a strong dependence on PDNP size.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laurie Freire Boullosa, Jinthe Van Loenhout, Tal Flieswasser, Christophe Hermans, Celine Merlin, Ho Wa Lau, Elly Marcq, Marlies Verschuuren, Winnok H. De Vos, Filip Lardon, Evelien L. J. Smits, Christophe Deben
Summary: The study aims to enhance the efficacy of auranofin (AF) as a cancer treatment by combining it with the PARP inhibitor olaparib (referred to as 'aurola'). High concentrations of AF and olaparib synergistically induced cytotoxicity in NSCLC and PDAC cell lines with low levels of mutant p53 protein. The combination strategy of oxidative stress induction with PARP inhibition could be a promising treatment for mutant p53 cancers.