Article
Cell Biology
Fumihiko Urabe, Nobuyoshi Kosaka, Yusuke Yamamoto, Kagenori Ito, Kurataka Otsuka, Carolina Soekmadji, Shin Egawa, Takahiro Kimura, Takahiro Ochiya
Summary: Bone metastases are a major concern for prostate cancer patients, leading to complications and decreased survival. Recent studies have found that extracellular vesicles (EVs) are involved in tumor progression. This study demonstrates that EVs from metastatic prostate cancer cells promote osteoclast formation, and the transmembrane protein CDCP1 is identified as an inducer of this process. Furthermore, CDCP1 expression on EVs may serve as a potential biomarker for detecting bone metastasis in prostate cancer.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Review
Biotechnology & Applied Microbiology
Kecheng Lou, Shangzhi Feng, Hui Luo, Junrong Zou, Guoxi Zhang, Xiaofeng Zou
Summary: Macrophages and their extracellular vesicles play significant roles in tumor microenvironment and cancer development, and show potential for clinical applications in immunomodulation, cancer therapy, infectious defense, and tissue repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, Research & Experimental
Chi Zhang, Xiaofan Yang, Tao Jiang, Chengqi Yan, Xiang Xu, Zhenbing Chen
Summary: Extracellular vesicles (EVs) released from cells have intercellular or inter-tissue communication abilities due to the bioactive molecules enclosed in their lipid bilayer membrane. Tissue-derived extracellular vesicles (Ti-EVs) directly isolated from the interstitial space of tissue can better reflect the physiological or pathological state of the tissue microenvironment. This review summarizes the isolation procedures of Ti-EVs and discusses their potential roles in understanding pathogenesis, guiding disease diagnosis and therapy, and exploring cell-free treatments.
Article
Nanoscience & Nanotechnology
Lihuan Shang, Qi Xie, Conglian Yang, Li Kong, Zhiping Zhang
Summary: In this study, it was found that cationic polymer nanoparticles could be excreted in an extracellular vesicle-coated form. They may pass through early endosomes, multivesicular bodies, and autophagic MVBs within cells, allowing for efficient intercellular transportation. This finding provided a proof-of-concept for utilizing EV-mediated transportation of nanoparticles, expanding their clinical application.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Lihuan Shang, Qi Xie, Conglian Yang, Li Kong, Zhiping Zhang
Summary: The interaction between nanoparticles and cells plays a crucial role in the therapeutic effects of nanomedicine. This study discovered that cationic polymer nanoparticles (cNPs) can be excreted in the form of extracellular vesicles (EVs). It was deduced that cNPs may pass through different compartments within cells. Furthermore, a high level of autophagy promotes the exocytosis process. These findings demonstrate the potential of using EV-coated nanoparticles as efficient biomimetic drug delivery systems.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Natalia Gebara, Yolanda Correia, Keqing Wang, Benedetta Bussolati
Summary: Angiogenesis, regulated by extracellular vesicles (EVs) from gestational tissues, plays a crucial role in healthy pregnancy and is dysregulated in pregnancy-related diseases like preeclampsia. The use of EVs from placental stem cells shows promise in clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Han Liu, Hao Zhang, Yafei Han, Yan Hu, Zhen Geng, Jiacan Su
Summary: This review provides an overview of the applications of bacterial extracellular vesicles (BEVs) in cancer therapy. They have low toxicity, strong drug loading capacity, and the ability to stimulate immune responses, showing great potential in the treatment of bone and soft tissue tumors.
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Jun-Jie Bai, Xuan Zhang, Xing Wei, Yu Wang, Cheng Du, Ze-Jun Wang, Ming-Li Chen, Jian-Hua Wang
Summary: Exosomes are crucial biomarkers for intercellular communication and disease diagnosis and treatment. This study presents a microfluidic chip called DEIC for isolating high-purity exosomes from small-volume biological samples. The chip utilizes elastoinertial separation to remove protein contaminants and achieves a 70.6% recovery and a 91.4% removal rate for proteins. The technique enables individual-vesicle-level biomarker analysis and shows potential for integration with downstream exosome analyses.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maxim L. Bychkov, Artem Kirichenko, Irina N. Mikhaylova, Alexander S. Paramonov, Evgeny Yastremsky, Mikhail P. Kirpichnikov, Mikhail A. Shulepko, Ekaterina N. Lyukmanova
Summary: Metastatic melanoma cells release extracellular vesicles (EVs) that can influence the morphology and homeostasis of normal keratinocytes, promoting tumor growth and migration. Additionally, EVs can inhibit the secretion of certain factors by keratinocytes, resulting in worse survival prognosis.
Review
Cell Biology
Su-Ran Li, Qi-Wen Man, Xin Gao, Hao Lin, Jing Wang, Fu-Chuan Su, Han-Qi Wang, Lin-Lin Bu, Bing Liu, Gang Chen
Summary: Extracellular vesicles (EVs) are lipid-bilayer membrane structures secreted by most cell types, acting as messengers through the transfer of lipids, proteins, and nucleic acids to influence cellular processes. Tissue-derived EVs (Ti-EVs) offer advantages such as tissue specificity and accurate reflection of the tissue microenvironment, playing important roles in intercellular communication and disease development insights. Current research on Ti-EVs is limited compared to body fluid or cell culture-derived EVs, highlighting the need for further exploration in basic and clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Chemistry, Multidisciplinary
Jing Cui, Yan-Jing Zhang, Xuan Li, Jia-Jiao Luo, Lei-Lei Zhao, Xin-Yue Xie, Wei Ding, Jing-Cong Luo, Ting-Wu Qin
Summary: Extracellular vesicles derived from tendon-derived stem cells were bound to collagen using a collagen binding domain, resulting in the fabrication of a bio-functionalized scaffold. The scaffold promoted stem cell proliferation, migration, and tenogenic differentiation in vitro, and also facilitated stem cell recruitment, collagen deposition, and biomechanical improvement in injured tendons, leading to functional tendon regeneration.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Oncology
Eshwari Dathathri, Khrystany T. Isebia, Fikri Abali, Martijn P. Lolkema, John W. M. Martens, Leon W. M. M. Terstappen, Ruchi Bansal
Summary: Prostate cancer is a common male malignancy with diverse clinical presentations and challenging treatment options. Liquid biopsy provides opportunities for early diagnosis and personalized treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Alberto Carretero-Gonzalez, Marta Hergueta-Redondo, Sara Sanchez-Redondo, Pilar Ximenez-Embun, Luis Manso Sanchez, Eva Ciruelos Gil, Daniel Castellano, Guillermo de Velasco, Hector Peinado
Summary: Brain metastases occur in nearly 40% of advanced cancer patients and are associated with poor prognosis. Liquid biopsy using extracellular vesicles (EVs) as biomarkers allows for noninvasive monitoring of brain metastases. This study found that decreased STAT3 activation and increased PD-L1 levels in plasma circulating EVs are associated with brain metastases in melanoma patients.
Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Endocrinology & Metabolism
Sofia Halin Bergstrom, Helena Jaremo, Maria Nilsson, Hanibal Hani Adamo, Anders Bergh
Article
Biochemistry & Molecular Biology
Vikte Lionikaite, Petra Henning, Christina Drevinge, Furqan A. Shah, Anders Palmquist, Pernilla Wikstrom, Sara H. Windahl, Ulf H. Lerner
Article
Pathology
Peter Hammarsten, Andreas Josefsson, Elin Thysell, Marie Lundholm, Christina Hagglof, Diego Iglesias-Gato, Amilcar Flores-Morales, Par Stattin, Lars Egevad, Torvald Granfors, Pernilla Wikstrom, Anders Bergh
Article
Oncology
Elin Thysell, Linda Vidman, Erik Bovinder Ylitalo, Emma Jernberg, Sead Crnalic, Diego Iglesias-Gato, Amilcar Flores-Morales, Paer Stattin, Lars Egevad, Anders Widmark, Patrik Ryden, Anders Bergh, Pernilla Wikstrom
MOLECULAR ONCOLOGY
(2019)
Article
Oncology
Gjendine Voss, Benedikta S. Haflidadottir, Helena Jaremo, Margareta Persson, Tina Catela Ivkovic, Pernilla Wikstrom, Yvonne Ceder
Article
Endocrinology & Metabolism
Erik Bovinder Ylitalo, Elin Thysell, Camilla Thellenberg-Karlsson, Marie Lundholm, Anders Widmark, Anders Bergh, Andreas Josefsson, Maria Brattsand, Pernilla Wikstrom
Article
Oncology
Ilona Dudka, Elin Thysell, Kristina Lundquist, Henrik Antti, Diego Iglesias-Gato, Amilcar Flores-Morales, Anders Bergh, Pernilla Wikstrom, Gerhard Grobner
Article
Oncology
Sofia Halin Bergstrom, Stina Rudolfsson, Marie Lundholm, Andreas Josefsson, Pernilla Wikstrom, Anders Bergh
Summary: The presence of high-grade tumors can increase the size and proliferation rate of low-grade tumors in experimental models and patient samples, as well as alter the surrounding microenvironment by increasing blood vessel density and macrophage density.
JOURNAL OF PATHOLOGY
(2021)
Article
Oncology
Anna-Maja Aberg, Sofia Halin Bergstrom, Elin Thysell, Lee-Ann Tjon-Kon-Fat, Jonas A. Nilsson, Anders Widmark, Camilla Thellenberg-Karlsson, Anders Bergh, Pernilla Wikstrom, Marie Lundholm
Summary: This study found that high levels of monocytes, S100A9, and S100A12 in prostate cancer patients were associated with poor outcomes, particularly in those with metastases at diagnosis.
Article
Oncology
Erik Bovinder Ylitalo, Elin Thysell, Mattias Landfors, Maria Brattsand, Emma Jernberg, Sead Crnalic, Anders Widmark, Magnus Hultdin, Anders Bergh, Sofie Degerman, Pernilla Wikstrom
Summary: Integrated epigenome and transcriptome analysis identified pronounced hypermethylation in malignant compared to non-malignant areas of localized prostate tumors. Metastases showed an overall hypomethylation in relation to primary PC, including CpGs in the AR promoter accompanied with induction of AR mRNA levels. We identified a Methylation Classifier for Androgen receptor activity (MCA) signature, which separated metastases into two clusters (MCA positive/negative) related to tumor characteristics and patient prognosis.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Ake Lundwall, Erik Bovinder Ylitalo, Pernilla Wikstroem, Maria Brattsand
Summary: KLK4 and its splice variant KLK4T2, as well as the pseudogene KLKP1, show differential expression patterns in prostate tissue, with potential implications for prostate cancer and benign prostatic hyperplasia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Elin Thysell, Linda Kohn, Julius Semenas, Helena Jaremo, Eva Freyhult, Marie Lundholm, Camilla Thellenberg Karlsson, Jan-Erik Damber, Anders Widmark, Sead Crnalic, Andreas Josefsson, Karin Welen, Rolf J. A. Nilsson, Anders Bergh, Pernilla Wikstrom
Summary: This study classified prostate cancer bone metastases into three subtypes (MetA-C) based on differential gene expression, with most metastases being heterogeneous for these subtypes. MetA was found to be the most common subtype, while MetB was significantly enriched in castration-resistant samples and liver metastases, and was consistently associated with poor prognosis. The MetB subtype demonstrated specific genetic aberrations and provided independent prognostic value.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Pernilla Wikstrom, Sofia Halin Bergstrom, Andreas Josefsson, Julius Semenas, Annika Nordstrand, Elin Thysell, Sead Crnalic, Anders Widmark, Camilla Thellenberg Karlsson, Anders Bergh
Summary: This study found that the subtype of bone metastases and patient outcome after androgen deprivation therapy (ADT) can be predicted by analyzing the characteristics of the primary tumor. High cell proliferation, low PSA expression, and low AR expression in stromal cells in the primary tumor are associated with poor prognosis and the development of metastases enriched for the aggressive subtype.
Article
Urology & Nephrology
Johan Wanman, Kasim Abul-Kasim, Julius Semenas, Elin Thysell, Anders Bergh, Pernilla Wikstrom, Sead Crnalic
Summary: This study explores the presence and prognostic value of myeloma-like prostate cancer bone metastases and its relation to clinical, molecular, and morphological prognostic markers. The myeloma-like metastatic pattern is associated with poor survival and neurological outcomes after surgery for metastatic spinal cord compression.
EUROPEAN UROLOGY OPEN SCIENCE
(2023)
