Article
Biochemistry & Molecular Biology
Lisa Marie Schmitz, Felix Hageneier, Katrin Rosenthal, Tobias Busche, David Brandt, Jorn Kalinowski, Stephan Luetz
Summary: Cytochrome P450 monooxygenases play a major role in drug metabolism, and microbial P450s offer advantages in drug synthesis. Actinosynnema mirum strain has been found to have the ability to catalyze reactions on a wide range of substrates, with one substrate, ritonavir, being studied for its metabolic mechanism.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Wadzani Palnam Dauda, Daji Morumda, Peter Abraham, Charles Oluwaseun Adetunji, Shakira Ghazanfar, Elkanah Glen, Shittu Emmanuel Abraham, Grace Wabba Peter, Israel Ogwuche Ogra, Ulasi Joseph Ifeanyi, Hannatu Musa, Mawuli Kwamla Azameti, Bilal Ahamad Paray, Aneela Gulnaz
Summary: This study investigates the diversity, abundance, evolutionary relationships, and cellular localization of 372 cytochrome P450 in 13 Alternaria species. It reveals the expansion of cytochrome P450s in these fungal genera and provides insights into their biology and toxigenic potentials.
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: The study identified and characterized a novel Spodoptera litura P450 gene, CYP321B1, which plays a crucial role in tannin detoxification and metabolism in insects. By conducting RNA interference and metabolome analysis, it was confirmed that silencing CYP321B1 significantly affects weight gain under tannin induction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Bridget Valeria Zinhle Nkosi, Tiara Padayachee, Dominik Gront, David R. Nelson, Khajamohiddin Syed
Summary: This study reveals the distinct characteristics of gene pools in the Bacteroidetes and Firmicutes bacterial groups, particularly in terms of bacterial families, ferredoxins, and secondary metabolites. These differences may contribute to the contrasting health effects of these two bacterial groups on the host.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Lihong Jiang, Lei Huang, Jin Cai, Zhinan Xu, Jiazhang Lian
Summary: This article discusses the strategies for improving the expression and activity of eukaryotic P450s in yeast, followed by examples of P450s involved in biosynthetic pathway engineering.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Ntokozo Minenhle Zondo, Tiara Padayachee, David R. R. Nelson, Khajamohiddin Syed
Summary: This study comprehensively analyzed P450 enzymes and their role in natural metabolite synthesis in 2666 mycobacterial species. The study revealed that mycobacterial species have distinct P450 profiles, indicating that lifestyle impacts P450 content in their genome and plays a key role in organisms' adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ntombizethu Nokuphiwa Msomi, Tiara Padayachee, Nomfundo Nzuza, Puleng Rosinah Syed, Justyna Dorota Krys, Wanping Chen, Dominik Gront, David R. Nelson, Khajamohiddin Syed
Summary: The impact of lifestyle on shaping the genome content of different bacterial species in terms of cytochrome P450 enzymes (CYPs/P450s) varies, with only a few Gammaproteobacterial species showing P450 enzymes involved in secondary metabolism. Lifestyle influences the diversity and quantity of P450 enzymes in bacteria, as observed in this comprehensive analysis of P450s in Gammaproteobacteria.
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: In this study, a P450 gene CYP6AB12 was identified and characterized from Spodoptera litura, an insect species. The gene was found to play an important role in the detoxification and metabolism of gossypol, a plant allelochemical. Silencing the gene resulted in lowered expression levels and increased sensitivity to gossypol. These findings confirm the broad detoxification and metabolism abilities of P450s and provide a molecular basis for further research on detoxification metabolism and pest control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Review
Entomology
Ralf Nauen, Chris Bass, Rene Feyereisen, John Vontas
Summary: Insect cytochrome P450 monooxygenases (P450s) play important physiological roles and are particularly involved in the detoxification of xenobiotics. Recent advances in insect genomics and functional approaches have shed light on the evolution of insect P450s, their regulation mechanisms, and the genetic alterations leading to insecticide resistance. Additionally, studies on the sensitivity of beneficial insects to insecticides have provided valuable knowledge for managing P450-mediated resistance and safeguarding their health.
ANNUAL REVIEW OF ENTOMOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Xueke Gao, Xiangzhen Zhu, Chuanpeng Wang, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Junyu Luo, Jinjie Cui
Summary: This study characterized AgoCYP6CY19, a CYP gene in Aphis gossypii, and found that its expression is related to developmental stages and host specialization. Silencing AgoCYP6CY19 increased mortality and reduced fecundity in aphids. Additionally, AgoCYP6CY19 was induced by certain plant substances. These findings highlight the importance of AgoCYP6CY19 in detoxification and metabolism processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Praveen Khatri, Owen Wally, Istvan Rajcan, Sangeeta Dhaubhadel
Summary: Cytochrome P450 monooxygenases (P450) play important roles in the catalytic conversion of biological compounds in plants. In this study, 346 P450 enzymes encoded by 317 genes were identified in soybean, and their composition, structure, expression, and distribution were compared. Analysis of transcriptomic datasets and co-expression analysis identified some P450 enzymes that may be involved in partial resistance against Phytophthora sojae. This study provides new insights into the functions of P450 enzymes in soybean metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jing Shen, Qian Yang, Lubo Hao, Lingling Zhang, Xuefeng Li, Mingqi Zheng
Summary: The newly discovered gene CYP77B34 was found to mediate Descurainia sophia L. resistance to tribenuron-methyl, and may have contributed to cross-resistance to other herbicides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Yifeng Zhang, Lin Ma, Ping Su, Luqi Huang, Wei Gao
Summary: Terpenoids, as the largest family of natural products, play significant roles in medicine, agriculture, cosmetics, and food. Traditional extraction methods have low yields, waste resources, and are not suitable for endangered species. Modern biotech approaches are promising in discovering and characterizing plant terpenoid-related P450 enzymes, and metabolic engineering strategies can be employed to increase terpenoid production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Entomology
Weixing Zhang, Hongfang Wang, Zhenguo Liu, Ying Wang, Baohua Xu
Summary: The gene AccCYP4AV1, isolated from Apis cerana cerana, shows high expression in the midgut and responds to stressors like low temperature and ultraviolet radiation. However, it is downregulated by high temperature and chemical stressors. The protein encoded by AccCYP4AV1 is believed to be involved in defense against abiotic stress damage.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Matthew T. J. Halma, Jack A. Tuszynski, Gijs J. L. Wuite
Summary: The time and cost of developing new therapeutic drugs is a significant burden, involving computational screening, compound assays, and expensive clinical trials. This review highlights the value of dynamic conformational information obtained through optical tweezers for targeting "undruggable" proteins. Optical tweezers provide insights into the relationship between biological mechanisms and structural conformations, aiding in drug discovery. Developing workflows and tools for optical tweezers will improve efficiency, allowing for greater adoption in the biopharmaceutical industry. As a complementary tool, optical tweezers increase the number of potential drug candidates, enhance understanding of a target's complex structural dynamics, and elucidate compound-target interactions.
DRUG DISCOVERY TODAY
(2023)
Article
Biochemistry & Molecular Biology
Young-Ho Choi, Sung-Hun Park, Sung-Su Kim, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin
Summary: Post-translational modifications of chromatin structure by histone acetyltransferase (HATs) are crucial for gene expression regulation and various biological processes. The function of GNAT family HATs, especially Elp3, in the opportunistic human pathogenic fungus Aspergillus fumigatus remains largely unknown. In this study, we investigated the roles of Elp3 and GcnE in A. fumigatus by generating and characterizing individual null Delta elp3 and Delta gcnE mutants. The results showed that Elp3 and GcnE are associated with diverse biological processes and can be potential targets for controlling the pathogenic fungus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ntokozo Minenhle Zondo, Tiara Padayachee, David R. R. Nelson, Khajamohiddin Syed
Summary: This study comprehensively analyzed P450 enzymes and their role in natural metabolite synthesis in 2666 mycobacterial species. The study revealed that mycobacterial species have distinct P450 profiles, indicating that lifestyle impacts P450 content in their genome and plays a key role in organisms' adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Phelelani Erick Ngcobo, Bridget Valeria Zinhle Nkosi, Wanping Chen, David R. Nelson, Khajamohiddin Syed
Summary: In this study, a genome-wide analysis revealed the presence of 1204 P450s and 353 ferredoxins in archaea. The shared genes between bacteria and archaea, as well as their presence on plasmids and chromosomes, indicate a plasmid-mediated lateral transfer of these genes. Phylogenetic analysis suggests that archaeal P450s could have diverged from CYP109, CYP147, and CYP197. Based on these findings, it is proposed that all archaeal P450s have a bacterial origin and that the original archaea did not have P450s.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Oluwakemi Ebenezer, Pietro Comoglio, Gane Ka-Shu Wong, Jack A. Tuszynski
Summary: In the past two decades, the introduction of synthetic siRNAs into the cytoplasm has been found to effectively silence targeted genes, impacting gene expression and regulation. Extensive investments have been made in developing RNA therapeutics, including for diseases related to PCSK9. PCSK9 plays a crucial role in LDL-C uptake, and loss-of-function modifications of PCSK9 have shown significant clinical importance in reducing cholesterol levels and lowering the risk of CVD. Monoclonal antibodies and siRNA drugs targeting PCSK9 offer a promising option for managing lipid disorders and improving CVD outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Aarat P. Kalra, Somnath Biswas, Imani Mulrain, Michelle Wang, Jack A. Tuszynski, Gregory D. Scholes
Summary: Microtubules and actin filaments are protein polymers that have various energy conversion roles in cells. While their mechanochemical roles are well studied, their capabilities for photonic energy conversion are not well understood. This Perspective discusses the photophysical properties of protein polymers, the challenges and opportunities of integrating protein biochemistry with photophysics, and the potential of microtubules and actin filaments to serve as targets for photobiomodulation. It also presents the broad challenges and questions in the field of protein biophotonics, emphasizing the importance of understanding how protein polymers interact with light for biohybrid device fabrication and light-based therapeutics.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Oncology
Paola Vottero, Qian Wang, Marek Michalak, Maral Aminpour, Jack Adam Tuszynski
Summary: This study aims to identify the binding sites and modes of gatastatin and its derivatives through molecular docking simulations, and compare the computational binding free energy predictions with experimental results. The computational simulations did not show a strong preference for gamma tubulin, suggesting that further derivatization may be needed to increase its specificity.
Review
Cell Biology
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Summary: The fungus Aspergillus nidulans plays an important role in understanding the mechanisms of fungal growth and development. This review summarizes the regulators involved in the formation, maturation, dormancy, and germination of A. nidulans conidia.
Article
Microbiology
Heungyun Moon, Mi-Kyung Lee, Ilhan Bok, Jin Woo Bok, Nancy P. Keller, Jae-Hyuk Yu
Summary: This study reveals the gene regulatory networks governed by VeA and LaeA in Aspergillus nidulans, shedding light on the understanding of fungal biology and genetics. The findings demonstrate the vital roles of these global regulators in developmental and metabolic processes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Edward A. Rietman, Hava T. Siegelmann, Giannoula Lakka Klement, Jack A. Tuszynski
Summary: The identification of a target protein for inhibition interaction is crucial for successful cancer therapy. This paper proposes a modified topological-thermodynamic analysis method that focuses on selecting the most stable proteins and their subnetwork interaction partners with high expression levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Chemistry, Physical
Aarat P. Kalra, Somnath Biswas, Imani Mulrain, Michelle Wang, Jack A. Tuszynski, Gregory D. Scholes
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Review
Nutrition & Dietetics
Matthew T. J. Halma, Jack A. Tuszynski, Paul E. Marik
Summary: Cancer can be targeted metabolically through dietary and lifestyle changes, depriving cancer cells of glucose, and utilizing nutritional supplements and repurposed drugs, which can contribute to the prevention and treatment of cancer.
Review
Biochemistry & Molecular Biology
Namita Chutani, Sandhya Ragula, Khajamohiddin Syed, Suresh B. Pakala
Summary: MORC2, a recently discovered chromatin remodeler, plays a crucial role in cancer development by promoting cancer cell growth and migration, regulating gene transcription, and participating in DNA repair.
Article
Cell Biology
Jae-Yoon Kwon, Young-Ho Choi, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin, Eduardo A. Espeso
Summary: This study investigated the biological and regulatory functions of the MYST family HAT SasC in the pathogenic fungus Aspergillus fumigatus. The results showed that the deletion of sasC significantly reduced colony growth, fungal virulence, and gene expression in A. fumigatus.
Article
Microbiology
Jiao Pan, Xinyu Yang, Cuiting Hu, Tongtong Fu, Xiuyan Zhang, Zijun Liu, Yu Wang, Fengyu Zhang, Xiaoyuan He, Jae-Hyuk Yu
Summary: The study demonstrates the crucial role of the choC gene in the biosynthesis of phosphatidylcholines (PCs) and lipid metabolism in Aspergillus fumigatus, which is essential for fungal growth, development, cell viability, and pathogenicity.
MICROBIOLOGY SPECTRUM
(2023)