Article
Biotechnology & Applied Microbiology
Lu Shi, Tiantian Zhao, Lei Huang, Xiaomin Pan, Tianzhen Wu, Xin Feng, Taoli Chen, Jiamin Wu, Jianlou Niu
Summary: Our study found that similar to FGF19(WT), chronic administration of FGF19(Delta KLB) protected mice from cholestatic liver injury in two models. The therapeutic benefits of FGF19(Delta KLB) on cholestatic liver damage are attributed to reduced bile acid production, liver inflammation, and fibrosis. Importantly, FGF19(Delta KLB) did not induce tumorigenesis effects during prolonged treatment.
Article
Toxicology
Miao Yan, Lin Guo, Jiating Ma, Yan Yang, Tingli Tang, Bikui Zhang, Wei Zhou, Wei Zou, Zhenyan Hou, Hongmei Gu, Hui Gong
Summary: This study evaluated the hepatoprotective effects of liquiritin (LQ) in cholestatic mice. The results showed that LQ can attenuate cholestatic liver injury by regulating the expression of Sirt1/FXR/Nrf2 and bile acid transporters. Additionally, LQ had a global regulatory effect on hepatic miRNA expression.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jing Zeng, Jiangao Fan, Huiping Zhou
Summary: Chronic cholestatic liver diseases, such as primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), gradually progress to liver fibrosis, cirrhosis, and failure. Disruption of bile acid metabolism and intrahepatic circulation plays a crucial role in accelerating the progression of cholestatic liver diseases. Bile acids not only act as detergents for nutrition absorption but also function as key signaling molecules that regulate hepatic metabolism and immune responses. This review focuses on the role of bile acid-mediated signaling in cholestatic liver disease.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Pin-Ho Pan, Ya-Yu Wang, Shih-Yi Lin, Su-Lan Liao, Yu-Fang Chen, Wei-Chi Huang, Chun-Jung Chen, Wen-Ying Chen
Summary: 18-β-Glycyrrhetinic acid has promising hepatoprotective effects against cholestatic liver injury, improving liver histology and reducing oxidative stress, inflammation, apoptosis, impaired autophagy, and fibrosis.
Article
Biochemistry & Molecular Biology
Imran Sohail, Naila Malkani, Nimra Tahir, Ateeb Khalil, Rukset Attar, Sidra Mumtaz
Summary: This study evaluated the anti-cholestatic effect of silymarin against ANIT-induced cholestasis. The results showed that silymarin could alleviate hepatic injury by modulating the expression of genes involved in bile acid homeostasis.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Peng Wang, Peiqiang Yuan, Sen Lin, Heju Zhong, Xiaoling Zhang, Yong Zhuo, Jian Li, Lianqiang Che, Bin Feng, Yan Lin, Shengyu Xu, De Wu, Douglas G. Burrin, Zhengfeng Fang
Summary: This study investigated the intercorrelation between reproductive hormones and bile acids in pregnant sows and their fetuses. The newly identified PM4S and PM5S were found to be correlated with maternal and fetal bile acid homeostasis. The study confirmed the role of PM5S and PM4S as antagonists in bile acid homeostasis and FGF19 using pig hepatocytes. The pregnant sow is a promising animal model to study the pathogenesis of cholestasis during pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Tingting Yang, Xue Wang, Yi Zhou, Qiongna Yu, Cai Heng, Hao Yang, Zihang Yuan, Yingying Miao, Yuanyuan Chai, Ziteng Wu, Lixin Sun, Xin Huang, Bing Liu, Zhenzhou Jiang, Luyong Zhang
Summary: This study demonstrated that the selective agonist SEW2871 of S1PR1 could alleviate TJs damage in ANIT-induced cholestatic liver injury. The protective effect was mediated through the AMPK signaling pathway, suggesting S1PR1 as a potential therapeutic target for intrahepatic cholestatic liver injury.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Shizhang Wei, Tingting He, Xu Zhao, Manyi Jing, Haotian Li, Lisheng Chen, Ruimao Zheng, Yanling Zhao
Summary: In this study, the effect of FXR on cholestasis was investigated using an ANIT-induced cholestasis mouse model and FXR-/- mice. The results showed that FXR-/- mice developed spontaneous cholestasis and exhibited significant liver and ileal tissue damage. 16s rRNA gene sequencing analysis revealed gut microbiota dysbiosis in FXR-/- mice and ANIT-induced cholestasis mice, and untargeted metabolomics identified differential biomarkers associated with the pathogenesis of cholestasis caused by FXR knockout, including Lactobacillus_johnsonii_FI9785.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Nazmul Hasan, Jianglei Chen, David Matye, Huaiwen Wang, Wenyi Luo, Lijie Gu, Yung Dai Clayton, Yanhong Du, Tiangang Li
Summary: This study investigates the enhanced therapeutic efficacy of combining the apical sodium-dependent bile acid transporter inhibitor GSK2330672 and fibroblast growth factor-15 (FGF15) over-expression in Cyp2c70 KO mice. The combined treatment was more effective in alleviating hepatobiliary injury in female Cyp2c70 KO mice, but ineffective in male mice. It reduced bile acid pool and improved gut barrier function.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Rulin Wang, Tengteng Yuan, Jing Sun, Menghuan Yang, Yunna Chen, Lei Wang, Yanyan Wang, Weidong Chen, Daiyin Peng
Summary: Paeoniflorin alleviates estrogen-induced cholestasis by regulating bile acid transporters and synthesis enzymes, thereby maintaining bile acid homeostasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Peng Cao, Jun Gan, Sanlan Wu, Yixin Hu, Bin Xia, Xiaoyue Li, Hongan Zeng, Bingyu Cheng, Huifan Yu, Fei Li, Luqin Si, Jiangeng Huang
Summary: Tectorigenin (TG), a natural isoflavone, has protective effects on alpha-naphthylisothiocyanate-induced cholestatic liver injury by restoring abnormal markers and maintaining bile acid homeostasis through FXR-mediated bile acid efflux and Nrf2-mediated antioxidative pathways.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Plant Sciences
Tingting Qin, Muhammad Hasnat, Ziwei Wang, Hozeifa Mohamed Hassan, Yang Zhou, Ziqiao Yuan, Wenzhou Zhang
Summary: This study aimed to investigate the effects of geniposide (GE) on bile duct ligation (BDL)-induced liver fibrosis and the underlying mechanisms. The results showed that GE treatment protected mice from BDL-induced liver fibrosis by inhibiting NLRP3 inflammasome activation. Furthermore, the protective effect of GE was mediated through the activation of SIRT1 and subsequent deacetylation of FXR, which restored bile acid homeostasis and contributed to the observed hepaprotective effect.
Article
Nutrition & Dietetics
Ruixia Bao, Wei Wang, Beibei Chen, Jujie Pan, Qian Chen, Mengyang Liu, Dan Wang, Yuzheng Wu, Haiyang Yu, Lifeng Han, Yi Zhang, Tao Wang
Summary: This study investigates the association between uric acid and cholesterol metabolism and the effect of dioscin in preventing hyperuricemia-induced atherosclerosis. The results show that dioscin can ameliorate atherosclerosis in a hyperuricemic state and regulate the farnesoid X receptor (FXR) -small heterodimer partner (SHP) -7 alpha-hydroxylase (CYP7A1) signaling pathway in the liver. Tigogenin, a metabolite of dioscin, increases the conversion rate of cholesterols into bile acids. Clinical study also demonstrates that treatment with a dioscin-enriched preparation decreases serum cholesterol levels in individuals with hyperuricemia.
Article
Plant Sciences
Mengge Shi, Jie Tang, Tong Zhang, Han Han
Summary: Swertiamarin can alleviate ANIT-induced cholestasis by activating the farnesoid X receptor and bile acid excretion pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
YueHua Zhou, YunZhong Zhou, YiFei Li, Wei Sun, ZhaoLong Wang, Long Chen, Ye He, XiaoLong Niu, Jialiang Chen, Guangtao Yao
Summary: This study found that Da-Chai-Hu decoction (DCHD) treatment can improve the condition of cholestatic liver injury by altering the metabolism of bile acids, including reducing serum bile acid levels, relieving inflammation, and improving pathological changes in liver tissue.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Applied
Xiu-Qin Zheng, Lei-Xin Song, Zhu-Zhen Han, Ying-Bo Yang, Yi Zhang, Li-Hua Gu, Li Yang, Gui-Xin Chou, Zheng-Tao Wang
Summary: In this study, five new triterpenoids were isolated from the fruit spikes of Prunella vulgaris L., along with 15 known triterpenoids. Their structures were elucidated, and their anti-thyroid cancer activities were evaluated. Three of the compounds showed apparent inhibitory activity against thyroid cancer.
NATURAL PRODUCT RESEARCH
(2023)
Article
Plant Sciences
Hao Qiu, Lei-Xin Song, Ying-Bo Yang, Si-Yu Zhang, Zhu-Zhen Han, Zheng-Tao Wang, Li Yang
Summary: Two undescribed stilbenoid diglycosides, dendrosonside A and dendrosonside B, were isolated from the stems of Dendrobium 'Sonia'. Their structures were elucidated and they showed inhibition of nitric oxide production.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2023)
Article
Chemistry, Analytical
Yuangui Yang, Lvye Liu, Zhengtao Wang, Yan Jin, Yanhai Zhang
Summary: An automated on-line solid-phase extraction (SPE) high-performance liquid chromatography (HPLC) method was developed for the determination of six protoberberine alkaloids in compound Chinese medicines containing Coptidis Rhizoma and Phellodendri Cortex. The method effectively removed interferences from samples and successfully quantified the target alkaloids using ion pair reverse phase high-performance liquid chromatography.
ANALYTICAL LETTERS
(2023)
Article
Agriculture, Multidisciplinary
Maoqi Hou, Feng Nie, Jianing Zhao, Zhengcai Ju, Li Yang, Qingzhong Wang, Shujuan Zhao, Zhengtao Wang
Summary: In this study, six UGTs were characterized, two of which were novel. PnUGT71A3 catalyzes the glycosylation of protopanaxatriol (PPT) and F1 to form Rh1 and Rg1, respectively, and also the glycosylation of protopanaxadiol (PPD)-type Rg3 to generate Rd. PnUGT94M1 regioselectively catalyzes the rhamnosylation of the C6 glucose of PPT-type ginsenosides Rg1 and Rh1, generating ginsenosides Re and Rg2, respectively, and is UDP-beta-L-rhamnose (UDP-Rha)-dependent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Yuqi Mei, Linnan Li, Linghong Fan, Wenxiang Fan, Longchan Liu, Fangli Zhang, Zhizhi Hu, Kang Wang, Li Yang, Zhengtao Wang
Summary: This article systematically summarizes the history, stereochemistry, ethnopharmacology, and quality assessment of borneol, providing valuable information and strategies for its rational application and development.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Jieyu Zhang, Yan Chen, Wenxiang Fan, Linnan Li, Yueming Ma, Zhengtao Wang, Rong Shi, Li Yang
Summary: The combined efficacy of Plantago asiatica L. seed and Coptis chinensis Franch. rhizoma is better than either herb alone, likely due to herb-herb interactions affecting potency. The active components in Plantago, geniposidic acid (GPA), acteoside (ACT), and plantagoamidinic acid A (PLA), and the active component in Coptis, berberine (BBR), were found to have competitive interactions driven by transporters rOCT2 and rMATE1. These interactions affect the transport and excretion of compounds and lead to changes in efficacy after co-administration.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Yuangui Yang, Yan Jin, Yanhai Zhang, Zhengtao Wang
Summary: Considering the increasing demand for Panax notoginseng-based medicines and health products, it is crucial to analyze the chemical differences between its root and rhizome. This study successfully identified five characteristic markers and found that their content in the rhizome was significantly higher than in the root.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Yaru Wang, Yunxia Shang, Fengyu Tang, Kun Qiu, Xiaohui Wei, Zhengtao Wang
Summary: In this study, self-double-emulsifying drug delivery system enteric-coated capsules (PNS-SDE-ECC) were used to enhance the oral bioavailability and anti-inflammatory effects of Panax notoginseng saponins (PNS). PNS-SDE-ECC significantly promoted the absorption of PNS in the intestinal tract and exhibited sustained release and stability. Compared to gastric capsules, PNS-SDE-ECC increased the relative bioavailability of NGR1, GRg1, GRe, GRb1, and GRd by 4.83, 10.78, 9.25, 3.58, and 4.63 times, respectively. Furthermore, PNS-SDE-ECC reduced OXZ-induced inflammatory damage in the colon by regulating cytokine expression.
Article
Cell Biology
Mengjuan Wei, Xinnan Gu, Han Li, Zhiyong Zheng, Zhimiao Qiu, Yuchen Sheng, Bin Lu, Zhengtao Wang, Lili Ji
Summary: This study investigates the role of EGR1 and CGA in liver repair and regeneration after APAP-induced hepatotoxicity. The results show that EGR1 deficiency aggravates liver injury and delays regeneration, while CGA promotes liver regeneration and repair by inducing EGR1 transcriptional activation.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Cell Biology
Duo Zhang, Albert M. Li, Guanghui Hu, Menggui Huang, Fan Yang, Lin Zhang, Kathryn E. Wellen, Xiaowei Xu, Crystal S. Conn, Wei Zou, Mark Kahn, Seth D. Rhoades, Aalim M. Weljie, Serge Y. Fuchs, Nduka Amankulor, Daniel Yoshor, Jiangbin Ye, Constantinos Koumenis, Yanqing Gong, Yi Fan
Summary: Phosphoglycerate dehydrogenase (PHGDH)-mediated endothelial cell metabolism contributes to the formation of a hypoxic and immune-hostile vascular microenvironment, leading to glioblastoma resistance to CAR-T cell immunotherapy. Reprogramming endothelial metabolism by targeting PHGDH may improve T cell-based immunotherapy.
Article
Biochemistry & Molecular Biology
Deqing Ruan, Jingyi Yang, Qianfei Luo, Yanhong Shi, Lili Ding, Zhengtao Wang, Rui Wang, Li Yang
Summary: In this study, we found that goitrin, an alkaloid from Radix Isatidis, showed protective effects in LPS-induced septic shock by inhibiting caspase-11 non-canonical inflammasome pathway. This finding provides a new therapeutic strategy for the clinical treatment of septic shock.
Article
Cell Biology
Fan Yang, Duo Zhang, Haowen Jiang, Jiangbin Ye, Lin Zhang, Stephen J. Bagley, Jeffery Winkler, Yanqing Gong, Yi Fan
Summary: A compound called toosendanin (TSN) has been found to reprogram macrophages in order to enhance the immune response against glioblastoma (GBM) in mouse models. This compound reverses macrophage-mediated tumor immunosuppression, leading to increased T cell infiltration and activation, and reduced exhaustion.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Letter
Multidisciplinary Sciences
Shi-Jun He, Fan Yang, Jian-Ping Zuo, Ze-Min Lin
Article
Biochemistry & Molecular Biology
Yan Chen, Yingying Tang, Pei Liu, Jinyuan Wang, Weiqian Wang, Changhong Wang, Lili Ding, Aizhen Xiong, Li Yang, Zhengtao Wang
Summary: This study investigates the toxicity and toxicokinetic characteristics of a toxic PA N-oxide, senecionine N-oxide, using a UDP-glucuronosyltransferase 1A4 humanized mouse model (hUGT1A4 mouse model) and wild-type mice. Results show that senecionine N-oxide exposure caused less toxicity in hUGT1A4 mice, and N-glucuronidation occurred exclusively in hUGT1A4 mice. There were also differences in toxicokinetic characteristics between hUGT1A4 mice and wild-type mice, with enhanced clearance of senecionine N-oxide and senecionine in hUGT1A4 mice, leading to detoxification.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Plant Sciences
Wei Li, Tongxi Zhuang, Zixuan Wang, Xunjiang Wang, Longchan Liu, Yixuan Luo, Rufeng Wang, Linnan Li, Wendong Huang, Zhengtao Wang, Li Yang, Lili Ding
Summary: Red ginseng extracts (RGS) show potential in treating obesity and insulin resistance by improving glucose and lipid metabolism. This study demonstrated that RGS activates intestinal TGR5 to promote lipolysis and energy consumption by increasing circulating bile acid levels, and facilitates the transport of bile acids by promoting ASBT translocation.