Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Current and future prospects for CRISPR-based tools in bacteria

(2015) Michelle L. Luo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth

(2015) Nicholas R. Sandoval et al.   Biotechnology for Biofuels

Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system

(2015) Yi Wang et al.   JOURNAL OF BIOTECHNOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Harnessing Type I and Type III CRISPR-Cas systems for genome editing

(2015) Yingjun Li et al.   NUCLEIC ACIDS RESEARCH

Genomics of Clostridium tetani

(2015) Holger Brüggemann et al.   RESEARCH IN MICROBIOLOGY

Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile

(2015) Pierre Boudry et al.   mBio

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

Requirements for a successful defence reaction by the CRISPR–Cas subtype I-B system

(2015) Britta Stoll et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Technical guide for genetic advancement of underdeveloped and intractable Clostridium

(2014) Michael E. Pyne et al.   BIOTECHNOLOGY ADVANCES

Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

(2014) Michael E Pyne et al.   Biotechnology for Biofuels

Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia

(2014) Steven D Brown et al.   Biotechnology for Biofuels

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair inDrosophila

(2014) Scott J. Gratz et al.   GENETICS

A functional recT gene for recombineering of Clostridium

(2014) Hongjun Dong et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus

(2014) Ziga Zebec et al.   NUCLEIC ACIDS RESEARCH

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

(2014) Michelle L. Luo et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

CRISPR-Mediated Adaptive Immune Systems in Bacteria and Archaea

(2013) Rotem Sorek et al.   Annual Review of Biochemistry

Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum

(2013) Michael E Pyne et al.   Biotechnology for Biofuels

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

A simplified and efficient germline-specific CRISPR/Cas9 system forDrosophilagenomic engineering

(2013) Zachary L Sebo et al.   FLY

Rewriting a genome

(2013) Emmanuelle Charpentier et al.   NATURE

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Protospacer recognition motifs

(2013) Shiraz A. Shah et al.   RNA Biology

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

CRISPR-Cas systems and RNA-guided interference

(2013) Rodolphe Barrangou   Wiley Interdisciplinary Reviews-RNA

Precise Manipulation of the Clostridium difficile Chromosome Reveals a Lack of Association between thetcdCGenotype and Toxin Production

(2012) Stephen T. Cartman et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration

(2012) Mohab A. Al-Hinai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

(2012) John T. Heap et al.   NUCLEIC ACIDS RESEARCH

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

(2011) Bryan P Tracy et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

Development of an anhydrotetracycline-inducible gene expression system for solvent-producing Clostridium acetobutylicum: A useful tool for strain engineering

(2011) Hongjun Dong et al.   METABOLIC ENGINEERING

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

PHAST: A Fast Phage Search Tool

(2011) Y. Zhou et al.   NUCLEIC ACIDS RESEARCH

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression inClostridium perfringens

(2010) Andrea H. Hartman et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacers

(2010) Soley Gudbergsdottir et al.   MOLECULAR MICROBIOLOGY

A modular system for Clostridium shuttle plasmids

(2009) John T. Heap et al.   JOURNAL OF MICROBIOLOGICAL METHODS

The ClosTron: Mutagenesis in Clostridium refined and streamlined

(2009) John T. Heap et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

CRISPR-based adaptive and heritable immunity in prokaryotes

(2009) John van der Oost et al.   TRENDS IN BIOCHEMICAL SCIENCES

Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes

(2008) J. Carte et al.   GENES & DEVELOPMENT

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

(2008) S. Datta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE