Article
Biochemistry & Molecular Biology
Julia Schipke, Dagmar Juette, Christina Brandenberger, Chiara Autilio, Jesus Perez-Gil, Wolfgang Bernhard, Matthias Ochs, Christian Muehlfeld
Summary: The study found that different dietary patterns have a significant impact on lung function and pulmonary surfactant, with high-fat diets significantly affecting pulmonary surfactant function, and high-carbohydrate diets potentially leading to changes in phospholipid species. This provides new research directions for investigating pulmonary complications associated with obesity and type 2 diabetes.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Developmental Biology
Sumitra Tatapudy, Jobelle Peralta, Todd Nystul
Summary: In Drosophila ovarian epithelial follicle stem cells, the E2 ubiquitin ligase, Bendless, plays multiple roles in JNK signaling, differentiation, proliferation, and niche competition, with a relationship to hedgehog signaling pathway regulation.
Article
Biology
Xiaowen Ji, Jiajia Gao, Tian Wei, Li Jin, Guiran Xiao
Summary: This study reveals that the zinc transporter FOI is essential for fat body endoreplication and larval growth in Drosophila. Knockdown of FOI results in failure of fat body cells to reach normal size, decreased fat body size, and pupal lethality. The intracellular zinc depletion caused by FOI knockdown leads to oxidative stress, activation of the ROS-JNK signaling pathway, and inhibition of Myc expression, which is required for tissue endoreplication and larval growth in Drosophila.
Article
Multidisciplinary Sciences
Harmony R. Salzler, Vasudha Vandadi, Benjamin D. McMichael, John C. Brown, Sally A. Boerma, Mary P. Leatham-Jensen, Kirsten M. Adams, Michael P. Meers, Jeremy M. Simon, Robert J. Duronio, Daniel J. McKay, A. Gregory Matera
Summary: Polycomb complexes regulate cell type-specific gene expression programs through heritable silencing. H3K27me3 is essential for this process, while disruption of H3K36 interferes with H3K27me3. Mutants of Drosophila replication-dependent or replication-independent histone H3 genes maintain Polycomb silencing and reach later stages of development, but combined mutants display widespread Hox gene misexpression and fail to develop past the first larval stage. Chromatin profiling revealed that the H3.2K36R mutation disrupts H3K27me3 levels broadly, while H3.3K36R animals are mostly unaffected. Analysis of H3.3 distributions showed the enrichment of H3.3 at presumptive Polycomb response elements located outside of silenced domains.
Article
Biochemistry & Molecular Biology
Andrew C. Marshall, Jerry Cummins, Simon Kobelke, Tianyi Zhu, Jocelyn Widagdo, Victor Anggono, Anthony Hyman, Archa H. Fox, Charles S. Bond, Mihwa Lee
Summary: Demixing of proteins and nucleic acids into condensed liquid phases is a common mechanism in cellular organization. Proteins with low sequence complexity often form liquid-like condensates. RNA-binding proteins with prion-like regions drive condensate formation, but this study finds that the longer prion-like region of SFPQ attenuates condensation, serving a regulatory role in preventing aberrant condensate formation.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Neurosciences
Wei Yan, Hai Lin, Junwei Yu, Timothy D. Wiggin, Litao Wu, Zhiqiang Meng, Chang Liu, Leslie C. Griffith
Summary: By studying Drosophila, it was found that multiple subtypes of ring neurons work synergistically to regulate sleep amount and structure. Different subtypes of ring neurons have different effects, with some cell types promoting sleep and others promoting wakefulness. These findings contribute to a better understanding of sleep regulation mechanisms.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jonathan Mercier, Alexis A. Nagengast, Justin R. DiAngelo
Summary: The ability of animals to store lipids during periods of limited nutrients is crucial for survival. However, excessive storage of lipids caused by increased food availability in modern society leads to metabolic diseases. This study focuses on the role of SR protein kinases (SRPKs) in regulating lipid storage in Drosophila. Lowering the expression of SRPKs in the fat body of adult flies resulted in decreased triglyceride levels due to reduced fat storage per cell. This was accompanied by altered splicing of the b-oxidation gene, CPT1, leading to increased production of a more active enzyme and a lean phenotype in the flies. Additionally, decreased SRPK levels in the fat body also led to reduced eating, further contributing to the decreased triglyceride levels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Vaibhvi Vaibhvi, Sven Kuenzel, Thomas Roeder
Summary: The immune organs of the fruit fly, Drosophila, namely the fat body and hemocytes, show distinct responses to systemic infection. The fat body exhibits improved peptide synthesis and export, while hemocytes display enhanced phagocytic signatures.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Qiangqiang Jia, Sheng Li
Summary: This study elucidates the regulatory mechanism and physiological importance of fat body cell dissociation in pupal development and insect diapause. It provides insights into fat body biology and insect diapause regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Entomology
Dan-Yan Huang, Xiao-Ling Xia, Run Huang, Sheng Li, Dong-Wei Yuan, Su-Ning Liu
Summary: The study reveals a novel role of 20E-induced let-7 in controlling growth during larval-prepupal transition in insects, by modulating cell size and endoreduplication in the fat body, which is critical for larval growth.
Article
Biochemistry & Molecular Biology
Conner Nagle, Jasleen K. Bhogal, Alexis A. Nagengast, Justin R. DiAngelo
Summary: This study reveals the crucial role of Tnpo-SR proteins in regulating lipid storage in the Drosophila fat body, with decreased Tnpo-SR leading to triglyceride accumulation and increased starvation resistance. The lipid accumulation in Tnpo-SR-RNAi flies is due to increased triglyceride stored in each fat body cell, not increased food consumption. Altered splicing of CPT1 enzyme in Tnpo-SR-RNAi fat bodies suggests a decrease in lipid breakdown, contributing to excess triglyceride storage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Developmental Biology
Tadayoshi Murakawa, Tsuyoshi Nakamura, Kohei Kawaguchi, Futoshi Murayama, Ning Zhao, Timothy J. Stasevich, Hiroshi Kimura, Naonobu Fujita
Summary: The research team has developed a versatile toolkit for Drosophila, using a genetically encoded single-chain variable fragment, to analyze the function of a protein of interest (POI) in live tissues. By crossing different tagged flies, various analyses of the tagged POI in vivo can be conducted. The study reveals that downregulated lysosomal function in the fat body plays a crucial role in the metamorphosis of Drosophila.
Article
Cell Biology
Weiling Hong, Jian Zhang, Haiyang Dong, Yang Shi, Hongru Ma, Fengyan Zhou, Bingbing Xu, Ying Fu, Shixin Zhang, Shouqing Hou, Guo Li, Yandan Wu, Shuo Chen, Xiaohua Zhu, Wendong You, Feng Shi, Xiaofeng Yang, Zhefeng Gong, Jianhua Huang, Yongfeng Jin
Summary: The study provides evidence that splicing bias of Drosophila Dscam1 is crucial for the axonal wiring of mushroom bodies, with different bias types influencing development and non-repulsive signaling of MB.
Article
Biochemistry & Molecular Biology
Monika Riederer, Marlies Wallner, Natascha Schweighofer, Bianca Fuchs-Neuhold, Anna Rath, Andrea Berghold, Katharina Eberhard, Andrea Groselj-Strele, Wolfgang Staubmann, Marie Peterseil, Irmgard Waldner, Johannes A. Mayr, Michael Rothe, Sandra Holasek, Susanne Maunz, Elisabeth Pail, Moenie van der Kleyn
Summary: This study analyzed the maternal determinants of infant fat mass index (FMI) and fat-free mass index (FFMI), which are considered predictors for the later development of obesity. The researchers examined amino acids (AA) and oxylipins in maternal serum and breast milk (BM). They found associations between certain AA and oxylipins and infant FMI and FFMI. Maternal peripartum antibiotics also emerged as confounders in the study.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Neurosciences
Sizhe Lyu, Noritaka Terao, Hirofumi Nakashima, Motoyuki Itoh, Ayako Tonoki
Summary: Memory formation and sleep regulation are positively regulated by neuropeptide diuretic hormone 31 (DH31) in fruit flies. The expression of DH31 in specific neurons of the central complex and ventral lateral clock neurons in the brain is responsive to sleep regulation. Furthermore, DH31 acts on the DH31 receptor in the dFB neurons to regulate sleep and is also involved in forming intermediate-term memory through distinct neural pathways.
NEUROSCIENCE RESEARCH
(2023)
Article
Cell Biology
Xiankun Zeng, Lili Han, Shree Ram Singh, Hanhan Liu, Ralph A. Neumueller, Dong Yan, Yanhui Hu, Ying Liu, Wei Liu, Xinhua Lin, Steven X. Hou
Article
Genetics & Heredity
Di Wen, Crisalejandra Rivera-Perez, Mohamed Abdou, Qiangqiang Jia, Qianyu He, Xi Liu, Ola Zyaan, Jingjing Xu, William G. Bendena, Stephen S. Tobe, Fernando G. Noriega, Subba R. Palli, Jian Wang, Sheng Li
Article
Multidisciplinary Sciences
Shree Ram Singh, Xiankun Zeng, Jiangsha Zhao, Ying Liu, Gerald Hou, Hanhan Liu, Steven X. Hou
Article
Multidisciplinary Sciences
Ying Liu, Qinglan Ge, Brian Chan, Hanhan Liu, Shree Ram Singh, Jacob Manley, Jae Lee, Ann Marie Weideman, Gerald Hou, Steven X. Hou
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Qiangqiang Jia, Suning Liu, Di Wen, Yongxu Cheng, William G. Bendena, Jian Wang, Sheng Li
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Entomology
Panli Zhang, Shumin Liu, Hong-Sheng Song, Guozheng Zhang, Qiangqiang Jia, Sheng Li
JOURNAL OF INSECT PHYSIOLOGY
(2017)
Review
Entomology
Kang Li, Qiang-Qiang Jia, Sheng Li
Article
Multidisciplinary Sciences
Sheng Li, Shiming Zhu, Qiangqiang Jia, Dongwei Yuan, Chonghua Ren, Kang Li, Suning Liu, Yingying Cui, Haigang Zhao, Yanghui Cao, Gangqi Fang, Daqi Li, Xiaoming Zhao, Jianzhen Zhang, Qiaoyun Yue, Yongliang Fan, Xiaoqiang Yu, Qili Feng, Shuai Zhan
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Hanhan Liu, Jin Wang, Sheng Li
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2014)
Article
Entomology
Qiangqiang Jia, Xiaoxi Chen, Lixian Wu, Zifeng Ruan, Kang Li, Sheng Li
JOURNAL OF INSECT PHYSIOLOGY
(2018)
Article
Multidisciplinary Sciences
Dongwei Yuan, Shun Zhou, Suning Liu, Kang Li, Haigang Zhao, Shihui Long, Hanhan Liu, Yongfang Xie, Yunlin Su, Fengwei Yu, Sheng Li
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cardiac & Cardiovascular Systems
Ekanem N. Ekure, Adebowale Adeyemo, Hanhan Liu, Ogochukwu Sokunbi, Nnenna Kalu, Ariel F. Martinez, Babajide Owosela, Cedrik Tekendo-Ngongang, Yonit A. Addissie, Akinsanya Olusegun-Joseph, Desmond Ikebudu, Seth Berger, Maximilian Muenke, Zhe Han, Paul Kruszka
Summary: This study identified novel candidate genes and variants for CHD through exome sequencing, facilitating comparisons with previous studies in predominantly European cohorts. It represents an important first step in genomic studies of CHD in understudied populations.
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxi Chen, Liu Yang, Run Huang, Sheng Li, Qiangqiang Jia
Summary: The study showed the essential roles of matrix metalloproteinases (MMPs) in the nymph-adult transition of hemimetabolous insects, particularly highlighting the dominant role of PaMmp2 in the imaginal molting process of American cockroach. Inhibition of Mmp activity led to eclosion failure and reduced chitin content, affecting cell interactions and wing expansion.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Di Wen, Zhi Chen, Jiamin Wen, Qiangqiang Jia
Summary: In Drosophila melanogaster, different dietary sterols have differential effects on the expression of 20E biosynthetic genes and activate 20E primary response gene expression. Single sterol types are sufficient to support growth and development. These findings suggest that the enzymatic system responsible for the classical C-27 ecdysteroid biosynthetic pathway also participates in the conversion of C-28 and C-29 dietary sterols into C-28 ecdysteroids.