Article
Chemistry, Multidisciplinary
So Hee Nam, Joomyung Jang, Dae Hee Cheon, Seung-Eun Chong, Joon Hyung Ahn, Soonsil Hyun, Jaehoon Yu, Yan Lee
Summary: The designed pH-activatable cell-penetrating peptide dimer LH2 with histidine residues is able to penetrate cells specifically in weak acidic conditions, delivering paclitaxel effectively into triple-negative breast cancer cells. Both non-covalent complexes (PTX-LH2(M)) and covalent conjugates (PTX-LH2(C)) of LH2 show strong antitumor effects in a triple-negative breast cancer grafted mouse model at extremely low dosages, indicating potential for targeted drug delivery and therapy.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Physical
Yinglan Yu, Chang Zu, Dongsheng He, Yanan Li, Qinying Chen, Qian Chen, Huimin Wang, Ruijuan Wang, Birendra Chaurasiya, Jennica L. Zaro, Yiran Wang, Jiasheng Tu, Chunmeng Sun
Summary: Artemisinin, well known as an antimalarial drug, can also be used for inflammation and cancer treatment. Studies have shown that utilizing a reversibly activatable cell-penetrating peptide can enhance the concentration of artemisinin in tumors, leading to improved antitumor effects.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Anselm F. L. Schneider, Marina Kithil, M. Cristina Cardoso, Martin Lehmann, Christian P. R. Hackenberger
Summary: The challenge of delivering proteins into cells has been addressed by conjugating arginine-rich cell-penetrating peptides to the cell surface, enabling efficient delivery of protein-CPP conjugates. The use of thiol-reactive cell-penetrating peptides allows for simple co-incubation to deliver thiol-containing proteins, greatly simplifying protein and antibody delivery with minimal genetic or chemical engineering.
Review
Food Science & Technology
Gandhi Radis-Baptista
Summary: Cell-penetrating peptides (CPPs) are short polypeptides that have the ability to interact selectively with certain cell membranes, translocate across membranes, and accumulate in the cell cytoplasm and other subcellular compartments. They can intracellularly deliver various substances and nanomaterials, with potential applications in biomedicine and pharmaceutical biotechnology. Studies on venom peptides from animals have demonstrated their cell-penetrating activities and potential for modifying cell selectivity and target applications.
Article
Medicine, Research & Experimental
Haruya Yagi, Takumi Tomono, Yuma Handa, Natsuki Saito, Masami Ukawa, Kohei Miyata, Koichi Shigeno, Shinji Sakuma
Summary: This study investigates the use of cell-penetrating peptides anchored to polymeric platforms as an absorption enhancer for delivering biologics via mucosal routes. Results show that hyaluronic acid modified with L-octaarginine significantly enhances the mucosal absorption of protein drugs in the nasal cavities. The lung mucosa and oral mucosa were found to be suitable routes for mucosal absorption of biologics.
MOLECULAR PHARMACEUTICS
(2023)
Article
Food Science & Technology
Qiang Wu, Changqing Zhong, Guirong Zeng, Xu Zhang, Liping Xiang, Chang Wan, Yougui Yu
Summary: A superior ACE inhibitory peptide, QGVP, was successfully isolated from distilled spent grains of Chinese strong-flavor Baijiu. QGVP formed hydrogen bonds with the active site of ACE, reducing its alpha-helix structure content and causing inactivation. Moreover, QGVP showed no cytotoxicity and demonstrated potential antihypertensive effects.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Chemistry, Medicinal
Xin Deng, Ruiyao Mai, Chenyu Zhang, Dianbao Yu, Yichang Ren, Gang Li, Binbin Cheng, Ling Li, Zhiqiang Yu, Jianjun Chen
Summary: A novel PDC was synthesized to intelligently deliver paclitaxel, showing higher cellular uptake and cytotoxicity in experiments, with less toxicity to normal cells and the ability to overcome paclitaxel resistance. The results demonstrated that this new PDC had better in vivo anti-tumor efficacy than paclitaxel.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jianan Zhou, Yuting Cai, Tingting Li, Haixiang Zhou, Huilei Dong, Xia Wu, Zenghui Li, Wenjie Wang, Dan Yuan, Yun Li, Junfeng Shi
Summary: A novel topical delivery system, Gel 2_1 & Eylea, is developed using an aflibercept-loaded eye-drop hydrogel facilitated by cell-penetrating peptide 1. Gel 2_1 & Eylea demonstrates superior membrane permeability, increased stability, and prolonged drug retention time on the ocular surface, and significantly reduces the density of neovascularization with no adverse effects on normal corneoscleral limbal vessels in a rabbit CoNV model, showing high efficacy and biocompatibility. This research identifies a promising treatment for corneal neovascularization and potentially benefits other ocular neovascular diseases.
Article
Chemistry, Multidisciplinary
Aline D. Araujo, Huy N. Hoang, Junxian Lim, Jeffrey Y. W. Mak, David P. Fairlie
Summary: Summary: Aromatic groups play a crucial role in protein-membrane association and cell uptake. The electrostatic and hydrophobic properties of aromatic ring substituents influence the affinity towards phospholipid membranes. The combination of fluorine and sulfur substituents induces microdipoles, enhancing the cell uptake of peptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Patrick Knoell, Nikolas Hormann, Nguyet-Minh Nguyen Le, Richard Wibel, Ronald Gust, Andreas Bernkop-Schnuerch
Summary: The aim of this study was to develop nanostructured lipid carriers (NLCs) decorated with a polycationic cell-penetrating peptide (CPP) and coated with polyphosphates (PP) for charge conversion. The results showed that the modified NLCs had improved drug loading and endosomal escape properties, as well as increased mucus diffusion ability.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Pharmacology & Pharmacy
Csaba Bato, Ildiko Szabo, Zoltan Banoczi
Summary: In this study, the preparation and investigation of efficient cyclic CPPs are described. Different oligoarginines were designed to conjugate with rigid aromatic scaffolds or form disulfide bonds. The constructs displayed efficient internalization on cancerous cell lines. By utilizing more than one endocytic pathway, short peptides that can compete with well-known CPPs may be synthesized through cyclization.
Article
Pharmacology & Pharmacy
Mo'ath Yousef, Ildiko Szabo, Jozsef Muranyi, Francoise Illien, Dora Soltesz, Csaba Bato, Gabriella Toth, Gyula Batta, Peter Nagy, Sandrine Sagan, Zoltan Banoczi
Summary: This study demonstrates the preparation and investigation of efficient cell-penetrating peptides (CPPs) and explores the possibility of using unnatural aromatic amino acids to mimic the properties of tryptophan. These highly active CPPs have been successful in delivering antitumor drugs into specific cell lines.
Article
Multidisciplinary Sciences
Coralie M. Backlund, Rebecca L. Holden, Kelly D. Moynihan, Daniel Garafola, Charlotte Farquhar, Naveen K. Mehta, Laura Maiorino, Sydney Pham, J. Bryan Iorgulescu, David A. Reardon, Catherine J. Wu, Bradley L. Pentelute, Darrell J. Irvine
Summary: Covalent conjugation of antigens with cell-penetrating peptides enhances the potency of peptide vaccines by promoting antigen uptake by dendritic cells and accumulation in draining lymph nodes. This results in robust T cell priming and expansion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Keisuke Tsuchiya, Kanako Horikoshi, Minami Fujita, Motoharu Hirano, Maho Miyamoto, Hidetomo Yokoo, Yosuke Demizu
Summary: Researchers have developed hydrophobic CPPs based on the hydrophobic CPP TP10 by introducing an aliphatic carbon side chain. These stapled peptides maintained the hydrophobicity of TP10, enhanced helicity, and improved cell-penetrating efficiency. The stapled peptide F-3 with CF showed stable α-helical structure and highest cell-membrane permeability, while peptide F-4 exhibited remarkable stability in complex formation with pDNA for efficient intracellular delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Geoffrey W. Krissansen, Yi Yang, Fiona M. McQueen, Euphemia Leung, Deborah Peek, Yih Chih Chan, Cristin Print, Nicola Dalbeth, Megan Williams, Alan G. Fraser
INFLAMMATORY BOWEL DISEASES
(2015)
Article
Cell Biology
Yih-Chih Chan, David R. Greenwood, Yi Yang, Euphemia Leung, Geoffrey W. Krissansen
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2015)
Article
Chemistry, Multidisciplinary
Stefanie Papst, Anais F. M. Noisier, Margaret A. Brimble, Yi Yang, Yih-Chih Chan, Geoffrey W. Krissansen
AUSTRALIAN JOURNAL OF CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Glenn D. Bell, Shiva Reddy, Xueying Sun, Yi Yang, Geoffrey W. Krissansen
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Kristopher Montrose, Yi Yang, Geoffrey W. Krissansen
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Stefanie Papst, Anais F. M. Noisier, Margaret A. Brimble, Yi Yang, Geoffrey W. Krissansen
BIOORGANIC & MEDICINAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Stefanie Papst, Anais Noisier, Margaret A. Brimble, Yi Yang, Geoffrey W. Krissansen
BIOORGANIC & MEDICINAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Kristopher Montrose, Yi Yang, Xueying Sun, Siouxsie Wiles, Geoffrey W. Krissansen
SCIENTIFIC REPORTS
(2013)
Article
Immunology
Geoffrey W. Krissansen, Jaison Singh, Rupinder K. Kanwar, Yih-Chih Chan, Euphemia Leung, Klaus B. Lehnert, Jagat R. Kanwar, Yi Yang
EUROPEAN JOURNAL OF IMMUNOLOGY
(2006)
Article
Cell Biology
E Leung, KB Lehnert, JR Kanwar, Y Yang, Y Mon, HP McNeil, GW Krissansen
IMMUNOLOGY AND CELL BIOLOGY
(2004)
Article
Biotechnology & Applied Microbiology
JR Kanwar, RW Berg, Y Yang, RK Kanwar, LM Ching, XY Sun, GW Krissansen
CANCER GENE THERAPY
(2003)