Review
Pharmacology & Pharmacy
P. S. Russell, R. Velivolu, V. E. Maldonado Zimbron, J. Hong, I. Kavianinia, A. J. R. Hickey, J. A. Windsor, A. R. J. Phillips
Summary: The lymphatic system is increasingly important in various conditions, and fluorescent lymphatic imaging offers advantages over other methods. Successful delivery of fluorescent tracers to lymphatic vessels is a unique challenge due to the one-way drainage system. This review discusses the characteristics of fluorescent tracers and techniques used for high-quality lymphatic imaging, as well as potential areas of future research for improving tracer design.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xinxian Meng, Huizhu Li, Yuzhou Chen, Liman Sai, Sijia Feng, Ke Li, Wenjing Xi, Yunxia Li, Nguyen T. K. Thanh, Yueming Wang, Yan Wo, Xing Yang, Yuefeng Hao, Yixin Zhang, Jun Chen, Shaoqing Feng
Summary: This study proposed the use of PbS quantum dots for shortwave infrared (SWIR) luminescence imaging of lymphatic vessels in rat models, allowing comprehensive investigation of the lymphatic system and its function. The imaging technique demonstrated the normal lymphatic morphology in both the tail and hind limb, and easily identified the dominant lymphatic vessel and node in rats. Furthermore, it identified the lymphatic pump as segment contracting sections in rats, providing a direct feature for precise evaluation of lymphatic function. The SWIR luminescence imaging with PbS quantum dots also revealed the dynamic process of morphological and functional recovery in the lymphatic system of rat models. Overall, this imaging technique can improve lymphography and enhance our understanding of the lymphatic system and its function, facilitating the diagnosis of lymphatic dysfunction in the future.
Review
Pharmacology & Pharmacy
David Olmeda, Daniela Cerezo-Wallis, Elena Castellano-Sanz, Susana Garcia-Silva, Hector Peinado, Maria S. Soengas
Summary: Imaging of the lymphatic vasculature is crucial in understanding various diseases, particularly in cancer. Activation of lymphatic endothelial cells plays a key role in therapeutic strategies. Current research focuses on visualizing the lymphatic system through imaging technologies and targeting it with drugs.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Biochemical Research Methods
Fabrice C. Bernard, Jarred Kaiser, Sarvgna K. Raval, Zhanna Nepiyushchikh, Thanh N. Doan, Nick J. Willett, J. Brandon Dixon
Summary: The study utilized multichromatic NIR imaging with multiple tracers to assess microvascular clearance kinetics and pathways in different tissue spaces. The results demonstrated that NIR imaging enables simultaneous imaging of lymphatic and venous-mediated fluid clearance, measuring temporal changes in clearance rates and pathways.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Pathology
Georg Brunner, Meike S. Roux, Thomas Falk, Martina Bresch, Volker Boehm, Norbert Bloedorn-Schlicht, Thomas Meiners
Summary: Spinal cord injury (SCI) is associated with impaired venous vascular function and lymphatic abnormalities in human skin. The loss of neuronal control in chronic SCI affects the lymphatic system, leading to dysfunction and impaired development. These findings have important implications for understanding the pathogenesis of pressure ulcers and wound healing disorders in individuals with SCI.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Surgery
Yu Hashimoto, Shiko Kuribayashi, Keigo Sato, Yuki Itoi, Ko Nakata, Kengo Kasuga, Hirohito Tanaka, Hiroko Hosaka, Hayato Ikota, Marietta Iacucci, Toshio Uraoka
Summary: This study aims to validate the correlation between red dichromatic imaging (RDI) scores and the activity index of ulcerative colitis (UC). The results showed positive correlations between RDI scores and UCEIS, MES, and Nancy index. RDI scores had a higher correlation to histology compared to white-light imaging (WLI) scores.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Cardiac & Cardiovascular Systems
Abhay Srinivasan, Chris Smith, Ganesh Krishnamurthy, Fernando Escobar, David Biko, Yoav Dori
Summary: This study investigated the contribution of thoracic duct obstruction to lymphatic flow disorders. Pleural effusions, ascites, and protein-losing enteropathy were common symptoms associated with duct obstruction. Balloon dilation and other interventions were effective in alleviating the obstruction and improving symptoms.
CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS
(2023)
Article
Cell Biology
Shang Wang, Irina Larina
Summary: Developmental biologists have used imaging techniques to study dynamic cellular events, but some processes like mammalian fertilization and embryogenesis are difficult to directly image. This study used intravital window and optical coherence tomography to observe the transport of oocytes and embryos through the mouse oviduct, revealing novel location-dependent movement patterns and questioning established views in the field. The in vivo imaging approach offers potential for investigating reproductive physiology with live functional analysis.
Article
Gastroenterology & Hepatology
Jia-Yi Liu, Zhu-Yuan Ding, Zi-Yi Zhou, Sheng-Zhen Dai, Jie Zhang, Hao Li, Qiu Du, Ye-Yu Cai, Quan-Liang Shang, Yong-Heng Luo, En-Hua Xiao
Summary: Bile duct ligation in mice is a classical method for studying cholestatic fibrosis. This study aimed to evaluate the correlation between pathological changes and multiparameter MRI characteristics of liver fibrosis in a BDL mouse model. Results show that MRI parameters correlate with hepatic fibrosis rate and can be used for detecting cholestatic fibrosis.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jielei Gu, Xuke Chen, Yangshuo Jin, Mingke Liu, Qiong Xu, Xiaolin Liu, Zhenyu Luo, Sisi Ling, Ningning Liu, Shiming Liu
Summary: The study investigated the response of neonatal mouse heart under pressure overload induced by transverse aortic constriction with different-gauge needles. Results showed that mild pressure overload stimulated cardiomyocyte proliferation, adaptive hypertrophy, and angiogenesis, while moderate pressure overload led to pathological myocardial hypertrophy, fibrosis, and heart failure. Severe pressure overload caused poor survival outcomes in the mice. These findings provide new insights into cardiomyocyte proliferation and hypertrophy in neonatal mice under pressure overload.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Ryo Yano, Masashi Hirooka, Yohei Koizumi, Yoshiko Nakamura, Yusuke Imai, Makoto Morita, Yuki Okazaki, Takao Watanabe, Osamu Yoshida, Yoshio Tokumoto, Masanori Abe, Yoichi Hiasa
Summary: This study aimed to clarify the chronological changes in lymphatic vessels with liver disease progression. The maximum diameter of the cisterna chyli (CC) or terminal thoracic duct (tTD) was measured to evaluate the changes in lymphatic vessels, and these measurements were used to re-allocate unclassified patients according to the Baveno-VII criteria to diagnose clinically significant portal hypertension.
HEPATOLOGY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Da Guo, Aysen Gurkan Ozer, Marylka Yoe Uusisaari
Summary: The inferior olive (IO) is considered crucial for motor control as the only source of climbing fibers forming one of the cerebellar input pathways. A new surgical method has been developed to obtain high-level calcium imaging data from the IO network activity in living animals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Peripheral Vascular Disease
Jing Wu, Shi Fang, Ko-Ting Lu, Kelsey Wackman, Michal L. Schwartzman, Sergey Dikalov, Justin L. Grobe, Curt D. Sigmund
Summary: Interference with PPAR gamma in vascular smooth muscle leads to impaired vasodilation in systemic and renal vessels, resulting in salt-sensitive hypertension. Activation of the PGE2/EP3 signaling pathway may play a critical role in this process.
Article
Endocrinology & Metabolism
Shiuhwei Chen, ZhiJiang Huang, Harrison Kidd, Min Kim, Eul Hyun Suh, Shangkui Xie, Ebrahim H. Ghazvini Zadeh, Yan Xu, A. Dean Sherry, Philipp E. Scherer, Wen-hong Li
Summary: Appropriate insulin secretion is crucial for maintaining euglycemia and understanding the dynamics of insulin secretion at the cellular level in intact pancreas of living animals remains challenging. Through ZIMIR imaging, insulin/Zn2+ release of individual islet beta-cells can be tracked with high spatiotemporal resolution, revealing synchronized rhythmic secretion activity. Additionally, the use of chemogenetic approach and fluorescent granule zinc indicator show promise for selective and efficient islet cell labeling in living animals.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Flavia Neto de Jesus, Pierre-Yves von der Weid
Summary: In a mouse model of arthritis overexpressing TNF-alpha, the contractile activity of popliteal lymphatic vessels was altered, which may be related to the response of eNOS and iNOS.
Article
Pathology
Jun Asai, Satoshi Hirakawa, Jun-ichi Sakabe, Tsunao Kishida, Makoto Wada, Naomi Nakamura, Hideya Takenaka, Osam Mazda, Tetsumei Urano, Katsue Suzuki-Inoue, Yoshiki Tokura, Norito Katoh
AMERICAN JOURNAL OF PATHOLOGY
(2016)
Article
Dermatology
Takahiro Suzuki, Kazuki Tatsuno, Taisuke Ito, Jun-ichi Sakabe, Atsuko Funakoshi, Yoshiki Tokura
JOURNAL OF DERMATOLOGICAL SCIENCE
(2016)
Letter
Dermatology
Aiko Shiohama, Takashi Sasaki, Showbu Sato, Jun-ichi Sakabe, Taisuke Ito, Hideka Isoda, Yukari Zenke, Toshiaki Nakano, Tatsuo Maeda, Akira Ishiko, Kenji Kabashima, Yoshiki Tokura, Yoshihiko Mitsuhashi, Masayuki Amagai, Akiharu Kubo
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2016)
Article
Dermatology
T. Suzuki, J. Sakabe, K. Kamiya, A. Funakoshi, Y. Tokura
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2018)
Article
Dermatology
Tsuyoshi Yatagai, Takatoshi Shimauchi, Hayato Yamaguchi, Jun-ichi Sakabe, Masahiro Aoshima, Shigeki Ikeya, Kazuki Tatsuno, Toshiharu Fujiyama, Taisuke Ito, Toshiyuki Ojima, Yoshiki Tokura
JOURNAL OF DERMATOLOGICAL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Shigeki Ikeya, Jun-ichi Sakabe, Takahiro Yamada, Takafumi Naito, Yoshiki Tokura
SCIENTIFIC REPORTS
(2018)
Article
Dermatology
Tatsuki Fukuie, Ryuhei Yasuoka, Toshiharu Fujiyama, Jun-ichi Sakabe, Tomohide Taguchi, Yoshiki Tokura
PEDIATRIC DERMATOLOGY
(2019)
Article
Dermatology
Masahiro Aoshima, Pawit Phadungsaksawasdi, Shinsuke Nakazawa, Manami Iwasaki, Jun-ichi Sakabe, Takatsune Umayahara, Tsuyoshi Yatagai, Shigeki Ikeya, Takatoshi Shimauchi, Yoshiki Tokura
JOURNAL OF DERMATOLOGICAL SCIENCE
(2019)
Article
Allergy
Taisuke Ito, Takahiro Suzuki, Jun-ichi Sakabe, Atsuko Funakoshi, Toshiharu Fujiyama, Yoshiki Tokura
ALLERGOLOGY INTERNATIONAL
(2020)
Article
Oncology
Masaki Sano, Satoshi Hirakawa, Minoru Suzuki, Jun-ichi Sakabe, Mikako Ogawa, Seiji Yamamoto, Takanori Hiraide, Takeshi Sasaki, Naoto Yamamoto, Kazunori Inuzuka, Hiroki Tanaka, Takaaki Saito, Ryota Sugisawa, Kazuto Katahashi, Tatsuro Yata, Takafumi Kayama, Tetsumei Urano, Yoshiki Tokura, Kohji Sato, Mitsutoshi Setou, Hiroya Takeuchi, Hiroyuki Konno, Naoki Unno
Article
Allergy
Takatsune Umayahara, Takatoshi Shimauchi, Manami Iwasaki, Jun-ichi Sakabe, Masahiro Aoshima, Shinsuke Nakazawa, Tsuyoshi Yatagai, Hayato Yamaguchi, Pawit Phadungsaksawasdi, Kazuo Kurihara, Yoshiki Tokura
CLINICAL AND EXPERIMENTAL ALLERGY
(2020)
Article
Multidisciplinary Sciences
Shinsuke Nakazawa, Takatoshi Shimauchi, Atsuko Funakoshi, Masahiro Aoshima, Pawit Phadungsaksawasdi, Jun-ichi Sakabe, Sanki Asakawa, Noriyasu Hirasawa, Taisuke Ito, Yoshiki Tokura
SCIENTIFIC REPORTS
(2020)
Letter
Dermatology
Hui Mei Cheng, Tetsuya Honda, Ryota Asahina, Toshiya Miyake, Zachary Chow, Michio Tomura, Jun-Ichi Sakabe, Yoshiki Tokura, Kenji Kabashima
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Stephanie Claudinot, Jun-Ichi Sakabe, Hideo Oshima, Christele Gonneau, Thimios Mitsiadis, Daniel Littman, Paola Bonfanti, Geert Martens, Michael Nicolas, Ariane Rochat, Yann Barrandon
NATURE COMMUNICATIONS
(2020)
Letter
Dermatology
Yurika Masuda, Taisuke Ito, Yutaka Shimomura, Masaaki Ogai, Jun-Ichi Sakabe, Yoshiki Tokura
EUROPEAN JOURNAL OF DERMATOLOGY
(2018)