Article
Cell Biology
Sayaka Katsunuma, Hideru Togashi, Shuhei Kuno, Takeshi Fujita, Ken-Ichi Nibu
Summary: The study showed that the checkerboard-like cellular pattern in the cochlear auditory epithelia plays a crucial role in the survival and function of sensory hair cells, and its disruption can result in hearing loss.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huaping Tao, Zhiwei Bao, Zhengwei Fu, Yuanxiang Jin
Summary: Exposure to chlorothalonil (CTL) can induce structural and functional damage to the human IEB, leading to barrier dysfunction through the activation of the mitogen-activated protein kinase pathway.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Food Science & Technology
Xiaoling Chen, Diaoyun Luo, Gang Jia, Hua Zhao, Guangmang Liu, Zhiqing Huang
Summary: L-theanine, a bioactive component in tea leaves, was found to protect against lipopolysaccharide-induced intestinal tight junction damage in IPEC-J2 cells. It reversed the increase of reactive oxygen species production and lactate dehydrogenase release and decreased the mRNA expression of tight junction proteins related genes. This protective effect was mediated through the inhibition of the p38 MAPK-mediated NLRP3 inflammasome pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Cell Biology
Ashwinkumar Subramenium Ganapathy, Kushal Saha, Eric Suchanec, Vikash Singh, Aayush Verma, Gregory Yochum, Walter Koltun, Meghali Nighot, Thomas Ma, Prashant Nighot
Summary: This study revealed that increased clathrin-mediated endocytosis under starvation conditions promotes degradation of CLDN2 and enhances the TJ barrier. The association of CLDN2 with clathrin and AP2 as well as LC3 and lysosomes increased upon starvation, indicating the role of clathrin-mediated endocytosis in autophagy-induced CLDN2 degradation.
Article
Agriculture, Multidisciplinary
Yanting Qiu, Xiaorong Lin, Zhongzheng Chen, Bin Li, Yuanyuan Zhang
Summary: The study reveals the adverse effects of HMF on gastric epithelial cells, inducing cell death through apoptosis and increasing reactive oxygen species levels. It also highlights the destructive effect of HMF on cell tight junction integrity and the impact on gene expressions. These findings provide additional insights into the health risks of human gastric epithelium exposure to 5-HMF.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jie Song, Yao Hu, Lifang Wang, Changjin Ao
Summary: The study aimed to explore the potential of Artemisia annua ethanol extract (AAE) as a preventive agent for mastitis. The results showed that AAE pretreatment could alleviate inflammatory injury and tight junction abnormalities, which was associated with the inhibition of NF-kappa B signaling and the downregulation of CD36 levels.
Review
Immunology
Siti Muhamad Nur Husna, Hern-Tze Tina Tan, Norasnieda Md Shukri, Noor Suryani Mohd Ashari, Kah Keng Wong
Summary: Allergic rhinitis is a common disorder affecting up to 40% of the global population, characterized by impaired nasal epithelial barrier and crucial involvement of TJ molecules in its pathogenesis. Various regulatory factors have been identified, offering hope for the development of novel therapeutic approaches.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Takahiro Tsuzuno, Naoki Takahashi, Miki Yamada-Hara, Mai Yokoji-Takeuchi, Benso Sulijaya, Yukari Aoki-Nonaka, Aoi Matsugishi, Kyoko Katakura, Koichi Tabeta, Kazuhisa Yamazaki
Summary: This study found that ingestion of periodontal pathogens, especially Porphyromonas gingivalis, exacerbates experimental colitis by directly interacting with the intestinal epithelial barrier, leading to gastrointestinal inflammation.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Geriatrics & Gerontology
Qiongyan Ma, Xu You, Kaili Zhu, Xueyi Zhao, Ding Yuan, Ting Wang, Yaoyan Dun, Jie Wu, Dongming Ren, Changcheng Zhang, Haixia Zhao
Summary: Impaired tight junction function, reduced autophagy, and the activated p38MAPK/MMP9 pathway in Sertoli cells may be closely associated with age-related testicular dysfunction.
EXPERIMENTAL GERONTOLOGY
(2022)
Article
Cell Biology
Geetha Samak, Rupa Rao, Radhakrishna Rao
Summary: The study found that Lactobacillus casei and epidermal growth factor (EGF) can ameliorate osmotic stress-induced disruption of apical junctional complexes and barrier dysfunction in the intestinal epithelium through distinct signaling mechanisms.
Article
Cell Biology
Matthew Herrera, Patricia Molina, Flavia M. Souza-Smith
Summary: Chronic alcohol consumption increases susceptibility to inflammation, bacterial, and viral infections, while alcohol-induced lymphatic leakage may be attributed to disruption of junctional protein complexes in lymphatic endothelial cells. Phosphorylated p38 may play a key role in regulating alcohol-induced lymphatic endothelial cell permeability.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhenling Zhang, Qiuping Zhang, Fang Li, Yi Xin, Zhijun Duan
Summary: The study revealed that HO-1 protects the intestinal mucosal barrier integrity by inhibiting MAPK signaling, thus preventing tight junction dysregulation and epithelial cell damage.
BIOMOLECULES & THERAPEUTICS
(2021)
Article
Cell Biology
Soyoung Hwang, Peter Chang-Whan Lee, Dong Min Shin, Jeong Hee Hong
Summary: Spinophilin plays a crucial role in modulating the junctional network and cellular migration in lung cancer cells, affecting invasive ability and transporter activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Shen Zhuang, Ke Ming, Ning Ma, Jinrui Sun, Dehai Wang, Mingxing Ding, Yi Ding
Summary: A specific polysaccharide fraction (POPZ) from Portulaca oleracea L. was found to inhibit LPS-induced barrier injury and inflammatory responses in porcine intestinal epithelial monolayer, possibly through inactivating TLR4/NF-κB pathway and activating EGF/EGFR pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Review
Biochemical Research Methods
Paleerath Peerapen, Visith Thongboonkerd
Summary: The main problems of kidney stone disease include increasing prevalence and high recurrence rates globally. Despite efforts in the past, the pathogenic mechanisms remain unclear, with proteomics becoming essential to unravel these sophisticated disease mechanisms at various levels. This review provides an overview of kidney stone disease and updates on proteomics for better understanding of the disease mechanisms.
EXPERT REVIEW OF PROTEOMICS
(2021)
Review
Biochemical Research Methods
Rui Vitorino, Manisha Choudhury, Sofia Guedes, Rita Ferreira, Visith Thongboonkerd, Lakshya Sharma, Francisco Amado, Sanjeeva Srivastava
Summary: With the availability of genomic data and advancements in high-throughput sequencing technologies, peptidomics and proteogenomics have become important tools for studying peptides and proteins associated with gene mutations or genome remodeling. This article provides an overview of techniques for analyzing peptidomics and proteogenomics, as well as bioinformatics workflows for understanding differentially expressed peptides/proteins in disease pathogenesis. Additionally, it highlights the role of these technologies in deciphering disease pathways, cancer research, and biomarker discovery using biofluids, while discussing the challenges and future requirements for their clinical use.
EXPERT REVIEW OF PROTEOMICS
(2021)
Review
Biochemical Research Methods
Luis Perpetuo, Julie Klein, Rita Ferreira, Sofia Guedes, Francisco Amado, Adelino Leite-Moreira, Artur M. S. Silva, Visith Thongboonkerd, Rui Vitorino
Summary: Peptidomics is an emerging field that utilizes advanced techniques for analyzing peptides in biological samples. Computational methods, particularly artificial intelligence, are used to predict therapeutic peptides efficiently, which have become essential tools in protein science for discovering novel therapeutic peptides.
EXPERT REVIEW OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Sunisa Yoodee, Paleerath Peerapen, Sirikanya Plumworasawat, Visith Thongboonkerd
Summary: ARID1A is a newly discovered tumor suppressor gene commonly observed in human cells and frequently lost or defective in many cancers. Down-regulation of ARID1A in human endothelial cells directly induces angiogenesis by regulating angiopoietin-2 secretion and endothelial cell activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Thiago Seraphim, Nardin Nano, Yiu Wing Sunny Cheung, Siripat Aluksanasuwan, Carolina Colleti, Yu-Qian Mao, Vaibhav Bhandari, Gavin Young, Larissa Holl, Sadhna Phanse, Yuliya Gordiyenko, Daniel R. Southworth, Carol Robinson, Visith Thongboonkerd, Lisandra M. Gava, Julio C. Borges, Mohan Babu, Leandro R. S. Barbosa, Carlos H. Ramos, Philipp Kukura, Walid A. Houry
Summary: The R2TP complex, formed by RUVBL1 and RUVBL2 ATPases, associates with PIH1D1 and RPAP3 proteins and plays a role in promoting the formation of macromolecular complexes. RPAP3 is identified as the central subunit of R2TP, linking PIH1D1 and RUVBL1/2. The study provides insights into the structure and function of the R2TP complex.
Article
Oncology
Paleerath Peerapen, Kanyarat Sueksakit, Wanida Boonmark, Sunisa Yoodee, Visith Thongboonkerd
Summary: Loss of ARID1A is associated with higher grade of colorectal cancer. In this study, knockdown of ARID1A resulted in decreased cell death and senescence, enhanced cell proliferation, chemoresistance and invasion. Additionally, ARID1A knockdown led to the formation of polyploid giant cancer cells, increased cell aggregation and secretion of vascular endothelial growth factor (VEGF).
Article
Medicine, Research & Experimental
Paleerath Peerapen, Wanida Boonmark, Visith Thongboonkerd
Summary: This study investigated the protective effects and mechanisms of trigonelline against kidney stone formation. The results showed that trigonelline can reduce stone formation by inhibiting crystallization, crystal growth, and crystal-cell adhesion. Additionally, trigonelline downregulated crystal receptors on the apical membranes of renal tubular cells.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Chadanat Noonin, Paleerath Peerapen, Sunisa Yoodee, Chompunoot Kapincharanon, Rattiyaporn Kanlaya, Visith Thongboonkerd
Summary: This study clarifies the inhibitory roles of native human urinary THP in calcium oxalate monohydrate (COM) kidney stone formation. THP reduces COM crystal size, inhibits crystal growth and aggregation, and prevents crystal-cell adhesion. These inhibitory effects are predominantly mediated via the high affinity of THP with calcium ions.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Rattiyaporn Kanlaya, Chompunoot Kapincharanon, Kedsarin Fong-ngern, Visith Thongboonkerd
Summary: This study investigated the potential of epigallocatechin-3-gallate (EGCG) to reverse epithelial-mesenchymal transition (EMT) and restore normal cell morphology. The results demonstrated that EGCG effectively reversed EMT and inhibited tumor and fibrosis.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Toxicology
Chanettee Chanthick, Visith Thongboonkerd
Summary: This study found that hyaluronic acid promotes the formation of calcium oxalate kidney stones by enhancing crystal growth, adhesion to cells, and invasion through the extracellular matrix. These findings provide an explanation for the higher levels of hyaluronic acid in the urine of stone patients.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Rattiyaporn Kanlaya, Visith Thongboonkerd
Summary: This study demonstrates that persistent infection by E. coli may contribute to COM metabolic stone formation by translocation of ezrin to apical membranes, thereby promoting COM crystal-cell adhesion.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sunisa Yoodee, Paleerath Peerapen, Sirikanya Plumworasawat, Visith Thongboonkerd
Summary: This study found that heat-shock protein 90 (HSP90) and its four functional domains play important roles in the formation of kidney stones, promoting the crystallization, growth, aggregation, adhesion onto renal cells, and invasion through the extracellular matrix (ECM) of calcium oxalate monohydrate (COM) crystals.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Visith Thongboonkerd, Rattiyaporn Kanlaya
Summary: Exosomes are nanoscale vesicles derived from late endosomes and released from cells. They selectively encapsulate biomolecules and play roles in cell-cell communication. In kidney diseases, exosomes have divergent roles in pathogenesis, diagnostics, prognostics, and therapeutics.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sakdithep Chaiyarit, Visith Thongboonkerd
Summary: The research reveals that oxidative modification can reverse the modulatory activity of the urinary protein uromodulin (UMOD), promoting calcium oxalate stone formation. Oxidized forms of UMOD facilitate CaOx crystallization and crystal growth, while having no effect on crystal aggregation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Medicine, Research & Experimental
Sudarat Hadpech, Visith Thongboonkerd
Summary: CyPA is an immunophilin widely expressed in various cell types and tissues, with higher expression in proximal tubular epithelial cells in the kidney. Changes in CyPA expression and secretion play important roles in kidney diseases. Urinary CyPA shows promise as a diagnostic/prognostic marker in several kidney diseases. Understanding the theranostic roles of CyPA in kidney diseases may offer insights for designing new therapeutic interventions and prevention strategies.
