Article
Oncology
Christian Schwarzenbach, Larissa Tatsch, Juliana Brandstetter Vilar, Birgit Rasenberger, Lea Beltzig, Bernd Kaina, Maja T. Tomicic, Markus Christmann
Summary: Malignant glioma treatment relies on O-6-methylguanine induction by TMZ, but most glioma cells evade apoptosis and become senescent post-TMZ exposure. Inhibiting SCAP factors, c-IAP2 and Bcl-2, enhances TMZ toxicity and reduces senescent cell protection. BV6 and venetoclax act as senolytic agents in glioblastoma cells post-TMZ exposure, increasing cell death and showing synergistic effects.
Article
Entomology
Dionei Schmidt Muraro, Thaini M. Goncalves, Douglas Amado, Marcelo F. Lima, Holly J. R. Popham, Paula G. Marcon, Celso Omoto
Summary: This study aimed to investigate the susceptibility of Brazilian populations of Helicoverpa armigera to Helicoverpa armigera nucleopolyhedrovirus (HearNPV) and cross-resistance between HearNPV and insecticides. The results showed that the Brazilian populations of Helicoverpa armigera were susceptible to HearNPV, with low interpopulation variation. No cross-resistance was detected between HearNPV and flubendiamide or indoxacarb. These findings suggest that HearNPV can be an effective tool in integrated pest management and insect resistance management programs.
Article
Virology
Jianan Huang, Jing Hu, Longyu Zhang, Yang Zhao, Hongying Chen, Xiaodong Xu
Summary: In this study, the enhancement effect of hr4a on gene expression was investigated by adding hr4a to different types of viral promoters and the reporter gene egfp. The results showed that hr4a increased gene transcription controlled by early promoters, but had no enhancer effect on late promoters.
Review
Biotechnology & Applied Microbiology
Alexandra Marisa Targovnik, Jorge Alejandro Simonin, Gregorio Juan Mc Callum, Ignacio Smith, Franco Uriel Cuccovia Warlet, Maria Victoria Nugnes, Maria Victoria Miranda, Mariano Nicolas Belaich
Summary: Baculoviruses, as insect pathogens, have been widely used in biotechnological applications with various biological engineering features, including pest control, recombinant protein expression systems, mammalian gene delivery, and antigenic protein display systems. BEVS and BacMam technologies, due to their ease of use and availability as commercial systems, have been widely introduced in academia and the pharmaceutical industry as essential tools for developing biological products.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Entomology
Yunhui Kong, Lingling Sun, Yaling Tang, Jiashuang Li, Sheng Qin, Muwang Li
Summary: This study identified the key gene NPC-1 that controls the resistance of Bombyx mori to AcMNPV, revealing the important role of BmNPC1 in regulating virus infection ability and viral gene expression, providing new insights into the mechanism of viral resistance in silkworm.
Article
Virology
Hu Xue, Tian Lingqian, Li Jingqi, Zhou Yuan, Chen Xinwen, Mu Jingfang, Bai Huimin, Zhang Yongli, Wang Yun
Summary: The study revealed that AcMNPV utilizes bidirectional nucleocytoplasmic trafficking through nuclear import and export receptors to facilitate the nuclear accumulation of viral structural proteins, aiding in viral replication.
Article
Microbiology
Yong Qi, Shan-Shan Wang, Lu-Lin Li
Summary: Late gene expression of baculovirus depends on the virus-encoded RNA polymerase. The immediate-early gene ie1 plays essential roles in viral early gene expression, late gene expression, and DNA replication. IE1 can independently activate late gene expression, showing nonspecific transcription start sites.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Qianlong Yu, Minghui Wang, Xuemeng Ding, Jiachen Han, Hancheng Ma, Jie Li, Guiling Zheng, Bin Zhang, Changyou Li
Summary: Different Spodoptera exigua cell lines showed different apoptosis responses during Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infection. The activation of SeCaspase-1 to -6 and the inhibition of iap1, iap2, and p35 in Se-1 cells contributed to severe apoptosis, while the opposite occurred in Se-3 cells. The different expression of P35 was identified as an important reason for the apoptosis differences between the two cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Josephin Koschel, Gopala Nishanth, Sissy Just, Kunjan Harit, Andrea Kroeger, Martina Deckert, Michael Naumann, Dirk Schlueter
Summary: OTUB1 protein plays a role in preventing necroptosis in hepatocytes, protecting against liver damage and death, particularly in bacterial hepatitis and TNF challenge.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Multidisciplinary Sciences
Jianxiang Qiu, Zumei Liu, Zhixin Fang, Wenjiao Wu
Summary: Coiled coils (CCs) are important protein structural motifs and play crucial roles in protein structure and function. This study identified and analyzed 24 CC motifs in a large DNA virus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and found that these motifs are involved in various biological interactions and viral replication processes.
Article
Biochemistry & Molecular Biology
Kejie Li, Zhanqi Dong, Feifan Dong, Zhigang Hu, Liang Huang, Jie Wang, Peng Chen, Cheng Lu, Minhui Pan
Summary: Apoptosis is crucial for the normal growth, development, and immunity defense of living organisms. Viral infection triggers apoptosis in infected cells, but viruses have developed countermeasures to inhibit apoptosis in host cells. The study focuses on investigating the mechanism of how IAP2 induces apoptosis as well as the potential for creating resistant material through the knockout of BmNPV iap2.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Horticulture
Ariel Morel, Brendan Leigh, Delia Munoz, Primitivo Caballero, Pilar Medina, Beatriz Dader
Summary: Research indicates that Hyposoter didymator is capable of transmitting Autographa californica nucleopolyhedrovirus (AcMNPV) to Spodoptera littoralis and Spodoptera exigua, but the transmission efficiency is influenced by the parasitoid's host preference and the pathogenicity of the virus in each host.
Article
Virology
Jianan Huang, Huanlei Liu, Xiaodong Xu
Summary: The baculovirus expression vector system (BEVS) is widely used for producing recombinant proteins. However, there is a risk of homologous recombination between viral genome and functional baculovirus-derived elements associated with foreign genes, which can lead to defective viruses and decreased recombinant protein yields. Shortening the length of homologous fragments and separating them by point mutations can effectively reduce the unfavorable recombination.
Article
Biotechnology & Applied Microbiology
Mark R. Bruder, Marc G. Aucoin
Summary: We have developed a sensitive assay using CRISPR-Cas9-mediated gene targeting to evaluate the phenotypic impact of disrupting Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genes. By disrupting 13 AcMNPV genes, we evaluated their performance in terms of green fluorescent protein production and progeny virus yield. This assay provides an efficient strategy for scrutinizing AcMNPV gene function and developing an optimized recombinant baculovirus expression vector (rBEV) genome.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jin Zhao, Tingkai Teng, Jinwen Wang
Summary: This study identified a microRNA encoded by Autographa california multiple nucleopolyhedrovirus (AcMNPV) that regulates viral genes and host genes to affect virus infection. The microRNA, called AcMNPV-miR-4, downregulates the host gene alg-2, leading to an extended cell lifespan and reduced virus virulence in the early stage of infection. However, in the late stage, it increases the production of a specific virus structure.
JOURNAL OF GENERAL VIROLOGY
(2022)
