Article
Biochemical Research Methods
Isin T. Sakallioglu, Amith S. Maroli, Aline De Lima Leite, Robert Powers
Summary: This study introduces a rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method for mycobacterial lipidomics analysis, which achieves separation of various lipid classes specific to mycobacterium in a short runtime of 14 minutes. The method demonstrates high sensitivity and faster sample throughput compared to existing methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Felina Hildebrand, Harald Schoeny, Evelyn Rampler, Gunda Koellensperger
Summary: Lipidomics studies focus on identifying and quantifying lipids comprehensively. Reversed phase liquid chromatography (RP-LC) coupled with high resolution mass spectrometry (MS) is commonly used for lipid identification, but accurate lipid quantification remains challenging. We established a dual flow injection and chromatography setup to address the issue of solvent composition affecting ionization response. Our results confirmed that changing solvent conditions significantly influence ionization response and quantification biases. This finding was further supported by quantifying human plasma lipids and a panel of lipid species standards under different ionization conditions.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Yutaka Konya, Yoshihiro Izumi, Kenji Hamase, Takeshi Bamba
Summary: In this study, a novel method combining supercritical fluid chromatography (SFC) and tandem mass spectrometry was developed for the chiral analysis of natural amino acids. By optimizing the separation column and mobile phase, successful separation and analysis of various amino acid enantiomers were achieved, significantly reducing the analysis time. The method was validated through the analysis of a commercially available product, demonstrating its practicality in amino acid analysis.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Geochemistry & Geophysics
Eric T. Parker, Hannah L. McLain, Daniel P. Glavin, Jason P. Dworkin, Jamie E. Elsila, Jose C. Aponte, Hiroshi Naraoka, Yoshinori Takano, Shogo Tachibana, Hikaru Yabuta, Hisayoshi Yurimoto, Kanako Sakamoto, Toru Yada, Masahiro Nishimura, Aiko Nakato, Akiko Miyazaki, Kasumi Yogata, Masanao Abe, Tatsuaki Okada, Tomohiro Usui, Makoto Yoshikawa, Takanao Saiki, Satoshi Tanaka, Satoru Nakazawa, Yuichi Tsuda, Fuyuto Terui, Takaaki Noguchi, Ryuji Okazaki, Sei-ichiro Watanabe, Tomoki Nakamura
Summary: The hot water and acid extracts of Ryugu samples were analyzed for the presence of aliphatic amines and amino acids. Thirteen amino acids and four aliphatic amines were detected and quantitated in the samples. The presence of non-protein amino acids with abiotic origins and low levels of terrestrial amino acid contamination were observed in the samples. Multiple amino acid formation mechanisms were found to be active on the Ryugu parent body.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Bassam Lajin, Walter Goessler
Summary: The importance of element-selective detection with ICPMS has been enhanced by the introduction of tandem ICPMS/MS, enabling nonmetal speciation analysis. This study reports the first phosphorous speciation study in human urine using HPLC-ICPMS/MS, involving the determination of phosphoethanolamine. A simple derivatization procedure was employed for separation, and a novel chromatographic eluent, hexanediol, was used to address the challenge of eluting the hydrophobic derivative. The developed method showed fast chromatographic separation, no need for an isotopically labeled internal standard, and high accuracy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jun Young Park, Sarmila Amatya Shrestha, Sangwon Cha
Summary: The study investigated the feasibility of using reversed-phase liquid chromatography to separate ganglioside isomers, successfully achieving the isomer separation of disialogangliosides and trisialogangliosides for the first time. Crucial factors in the mobile-phase composition, such as the organic modifier acetonitrile and the ammonium salt additive ammonium bicarbonate, were identified to influence the differential retention and mass spectral response of the isomers.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Multidisciplinary Sciences
Diana Piol, Laura Tosatto, Emanuela Zuccaro, Eric N. Anderson, Antonella Falconieri, Maria J. Polanco, Caterina Marchioretti, Federica Lia, Joseph White, Elisa Bregolin, Giovanni Minervini, Sara Parodi, Xavier Salvatella, Giorgio Arrigoni, Andrea Ballabio, Albert R. La Spada, Silvio C. E. Tosatto, Fabio Sambataro, Diego L. Medina, Udai B. Pandey, Manuela Basso, Maria Plennuto
Summary: Spinal and bulbar muscular atrophy is caused by polyglutamine expansions in androgen receptor, leading to gain-of-function toxicity. This study investigates the phosphorylation of polyglutamine-expanded receptor and its role in neurodegeneration. The researchers find that certain kinases and phosphatases can modify the function and toxicity of the receptor, and targeting these enzymes can potentially be used as a therapeutic approach.
Article
Chemistry, Analytical
Yu-Ching Liu, Chao-Jung Chen
Summary: In this study, a fully automatic online 2D nano-LC-MS/MS system was developed for comprehensive proteomics analysis. The system utilized both high-pH and low-pH reverse phase LCs on a single LC instrument. Compared to conventional microflow 2D-LC, the online system showed superior coverage and identified a higher number of protein groups.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Zuzana Vankova, Ondrej Peterka, Michaela Chocholouskova, Denise Wolrab, Robert Jirasko, Michal Holcapek
Summary: The RP-UHPLC/MS method was developed to identify over 500 lipid species representing 26 polar and nonpolar lipid classes in human plasma. High-resolution mass spectrometry and chromatographic techniques were employed to accurately identify lipid species.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yumi Komori, Tomoya Niinae, Koshi Imami, Jun Yanagibayashi, Kenichi Yasunaga, Shinya Imamura, Masami Tomita, Yasushi Ishihama
Summary: We developed a bioinertized nanoflow LC/MS/MS system for sensitive analysis of phosphopeptides. Metal ion depletion from the mobile phase and prevention of indirect contact with metal components significantly improved the recovery of phosphopeptides. By inserting an online metal ion removal device, the identified phosphopeptides showed an average increase in peak areas of 9.9-fold overall and 77-fold for multiply phosphorylated peptides.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Energy & Fuels
Yueyi Xia, Chenfei Ma, Ran Ju, Chunxia Zhao, Fujian Zheng, Xiaoshan Sun, Zaifang Li, Chunyan Wang, Dejun Shi, Xiaohui Lin, Xin Lu, Guowang Xu
Summary: A method based on RPLC-HR MS using Orbitrap MS was proposed for the accurate characterization of heteroatom compounds in petroleum fractions. The technique successfully obtained detailed heteroatom class species and corresponding DBE vs carbon number distributions, providing a viable alternative to the expensive FT-ICR MS in petroleum production and processing.
Article
Biochemical Research Methods
Jeremy Molineau, Maria Hideux, Philippe Hennig, Sophie Bertin, Fabien Mauge, Eric Lesellier, Caroline West
Summary: A unified chromatography (UC) analysis method was developed for short-chain peptides, and compared to a reference reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) method for the analysis of 43 peptides. The two methods showed differences in orthogonality, success rate, and performance, with excellent complementarity observed in impurity profiling and separation of isomeric species.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Werner Schmitz, Corinna Koderer, Mohamed El-Mesery, Sebastian Gubik, Rene Sampers, Anton Straub, Alexander Christian Kubler, Axel Seher
Summary: This study establishes the murine cell line L929 as a model system for rapid analysis of methionine restriction (MetR) using liquid chromatography/mass spectrometry (LC/MS). The analysis of over 150 different metabolites defines a metabolic fingerprint and facilitates the identification of specific metabolites representing normal or MetR conditions. The study provides new insights into the effects of MetR at the cellular metabolic level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Hong Zhao, Yuying Wang, Liangjuan Zhao, Zhizheng Dong, Jiebo Mi, Huabing Zhao, Jing Wang, Jing Zeng, Hongbin Wang, Libing Wang
Summary: A novel strategy for pathogenic bacteria detection based on targeted analysis of characteristic peptides has been successfully applied to the detection of Staphylococcus aureus. The key to the success lies in the identification of reliable characteristic peptides that provide high specificity.
Article
Chemistry, Analytical
Denis K. Naplekov, Siddharth Jadeja, Alena Myslivcova Fucikova, Frantisek Svec, Hana Sklenarova, Juraj Lenco
Summary: A fast online method for acid cleavage of proteins directly in the mobile phase typically used for LC-MS analyses in proteomics is reported. The method requires only one manual step, significantly reducing sample transfer losses. The method is suitable for high-throughput proteomic workflows.
ANALYTICAL CHEMISTRY
(2023)
