期刊
SCIENCE IN CHINA SERIES B-CHEMISTRY
卷 52, 期 9, 页码 1362-1371出版社
SCIENCE PRESS
DOI: 10.1007/s11426-009-0213-x
关键词
acetohydroxyacid synthase; subunit interaction; surface plasmon resonance
资金
- National Key Project for Basic Research of China [2010CB126102]
- National Natural Science Foundation of China [20572053, 20421202, 20432010]
- Ministry of Education of China [104189]
Homologous and heterologous interactions between acetohydroxyacid synthase (AHAS) I and III from E. coli have been studied by surface plasmon resonance (SPR). The catalytic and regulatory subunits association for AHAS I (K (D) = 1.13 x 10(-7) M) was stronger than that for AHAS III (K (D) = 5.29 x 10(-7) M). A strong heterologous association between regulatory and catalytic subunits and heterologous activation of catalytic subunits were observed. SPR results combined with enzyme kinetics indicate that the reconstituted heterologous enzymes had similar kinetic properties as homologous enzymes, implying that the regulatory subunit of AHAS I could be replaced by the regulatory subunit of AHAS III and vice versa. This work may be useful to further understandings of the mechanism of regulation of AHAS.
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