期刊
RSC ADVANCES
卷 4, 期 94, 页码 52250-52255出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra08090d
关键词
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资金
- NSFC [21275041, 21235002]
- Foundation for Innovative Research Groups of NSFC [21221003]
- Hunan Provincial Natural Science Foundation of China [12JJ2010]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110161110009]
- PCSIRT [IRT1238]
A simple, label-free electrochemical biosensor based on a dopamine (DA) DNA aptamer immobilized on a Au electrode through the Au-S bond was developed. Methylene blue (MB) was used as the intercalating probe, and DA was sensitively and selectively detected. The developed electrochemical DA aptasensor showed a good linear response to DA from 5-150 nmol L-1 as well as having a low detection limit of 1.0 nM (S/N = 3). The biosensor also exhibited satisfactory selectivity and could be successfully used to detect DA in human blood, suggesting that the aptasensor is a promising application for the analysis of DA in real clinical samples.
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