期刊
RSC ADVANCES
卷 4, 期 40, 页码 20677-20685出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra00668b
关键词
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资金
- National Natural Science Foundation of China [21275158, 21105117]
- Innovation Projects of the Chinese Academy of Sciences [KZCX2-EW-206]
- 100 Talents Program of the Chinese Academy of Sciences
Molecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g(-1), excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R-2 = 0.9970) and a favorable detectability up to 1.5 ng mL(-1). Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research.
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