Article
Spectroscopy
Gong Bo, Li Bo-Yu, Yin Shou-Liang, Li Yue-Hua, Li Guang-Yue
Summary: This theoretical study investigated the sensing mechanism of a bifunctional fluorescent probe, revealing different geometries and transitions between S-0 and S-1 states. The study shed light on the mechanisms behind fluorescence quenching and strong fluorescence emission, providing insights into detecting hydroxyl radicals through changes in the fluorescence signal of the probe molecule.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Chao Wang, Wenchao Jiang, Davin Tan, Lu Huang, Jin Li, Qinglong Qiao, Priya Yadav, Xiaogang Liu, Zhaochao Xu
Summary: A quantum chemical calculation method was used to investigate factors affecting the twisted intramolecular charge transfer (TICT) process of fluorophores. Based on this research, a sensor array of hemicyanines with varied sensitivities and dynamic ranges was synthesized, enabling the observation of different stages of A beta aggregations. This approach will greatly facilitate the development of TICT-based fluorescent probes for various applications.
Article
Chemistry, Analytical
Bo Lin, Yanan Wei, Yanan Hao, E. Shuang, Yang Shu, Jianhua Wang
Summary: ENBT is a ratiometric fluorescent probe for pHmito imaging in living cells, showing accurate sensing of pH(mito) variations. The probe has low cytotoxicity and is suitable for pHmito imaging in live cells.
Article
Chemistry, Applied
Lulu Li, Dan He, Xiaodong Chen, Sanxiu Yang, Yunfei Yang, Aihua Gong, Kun Wang, Jing Qian, Lingliang Long
Summary: Visualizing minor pH fluctuations in subcellular organelles is important for studying physiological events in living organisms. In this study, a family of coumarin-indolenium based fluorescent pH probes were developed to visualize these minor pH fluctuations. The probes can be tuned by multiple approaches to accurately detect pH changes in different subcellular organelles. They have been successfully used to track pH fluctuation in mitochondria, lysosome, endoplasmic reticulum, as well as in zebrafish under high levels of exogenous CO2.
Article
Chemistry, Applied
Jian Qiang, Junqian Peng, Xin He, Shiyue Zheng, Sheng Lu, Xiaoqiang Chen
Summary: In this study, a new ratiometric pH probe, PHE-C, was developed to enhance the wavelength difference between the two peaks of the typical hemicyanine fluorophore without affecting its pKa value. The probe exhibited two emission maxima at 682 and 730 nm, with a larger wavelength difference compared to the unsubstituted hemicyanine. It demonstrated linear response to pH changes in the range of 5.5-7.5 and showed good performance in complex biological environments such as bovine serum solutions, making it a highly sensitive ratiometric probe for in vivo pH determinations.
Article
Chemistry, Applied
Kunal N. More, Seul-Ki Mun, Julie Kang, Jong-Jin Kim, Sung-Tae Yee, Dong-Jo Chang
Summary: Maintaining pH-homeostasis is crucial for cell survival, as abnormal pH in lysosomes can lead to various diseases. A series of novel pH-sensitive fluorescent sensors were developed based on a structure-pKa relationship study, showing potential for monitoring acidic lysosomes.
Article
Spectroscopy
Wenzhi Li, Yuxi Wang, Ruiling Zhang
Summary: In this study, the sensing mechanism of a triphenylamine-benzofuran-based probe (TBF-SS) towards H2Sn was investigated using the DFT method. The results showed that the inherent fluorescence quenching of the probe is mainly due to the twisted intramolecular charge transfer (TICT) effect. When the nitro fluorophenyl group is replaced by a hydroxyl group in the reaction with H2Sn, the TICT effect is eliminated, allowing the excited state to return to the ground state in a radiative way, leading to strong fluorescence emission.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Youfang Liu, Yingying Zhang, Jing Wang, Anquan Yang, Yanliang Zhao, Aying Zhou, Ranhua Xiong, Chaobo Huang
Summary: In this study, a high-performance fluorescence probe H4 based on intramolecular charge transfer (ICT) mechanism was developed. H4 exhibited outstanding sensitivity, short response time, low hemolysis effect, and excellent photo stability. It showed a good linear relationship in the pH range of 2.2-7.4 and could be used for real-time detection of H+ in food samples. The probe also demonstrated favorable behavior for detecting acid pH changes in living onion tissues and zebrafish.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Chenglu Zhang, Yiming Wang, Xiangling Li, Shiru Nie, Chang Liu, Yang Zhang, Jinghao Guo, Cui Liu
Summary: In order to detect Cu2+ and pH effectively, we synthesized a dual functional fluorescent probe NCNB based on naphthalimide. The probe is highly sensitive (LOD = 44.8 nM) and has a fast response time (within 150 s) for Cu2+. It can be used for qualitative and quantitative detection of Cu2+ in actual water samples as well as making pH fluorescent test paper with good effect. Moreover, the probe has been successfully used for imaging detection of exogenous Cu2+ in Caco-2 cells with low toxicity.
INORGANICA CHIMICA ACTA
(2023)
Review
Chemistry, Organic
Jinhua Xie, Le Wang, Xiqi Su, Joao Rodrigues
Summary: Coumarin-based derivatives have versatile properties and have been widely used in various applications, especially in biosensing. This review focuses on recent advances of coumarin-based fluorescent probes for metal ion imaging in living cells, highlighting representative examples and fluorescence response mechanisms such as PET, ICT, and FRET. Future perspectives in this field are also briefly mentioned.
CURRENT ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiangdong Ding, Bin Yang, Zhongling Liu, Meidi Shen, Zheyuan Fan, Xinghua Wang, Wei Yu
Summary: In this study, a novel near-infrared fluorescent probe was developed for highly selective detection of cysteine in living cells and mice. The probe exhibited superior sensitivity and low cytotoxicity, making it a promising tool for future clinical applications and research on cysteine-related diseases.
ANALYTICA CHIMICA ACTA
(2023)
Article
Spectroscopy
Hongbin Zhan, Hengwei Zhang, Yi Wang, Yaping Tao, Jing Tian, Xu Fei
Summary: This study investigated the fluorescence mechanism of HN-ClO and HN-ClO-F probes using DFT and TDDFT, showing distinct charge distribution and fluorescence characteristics in their excited states. The strong fluorescence of HN-ClO-F is attributed to the planar structure-induced charge coupling, while the weak fluorescence of HN-ClO is caused by inter-system crossing.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Sayed Zahid Nasim, Sehrish Sarfaraz, Faheem Jan, Muhammad Yar, Attiq Ur Rehaman
Summary: GGT is an important tumor biomarker that appears widely in tumor cells, and accurate imaging and detection of GGT activity in live cells, serum, and pathological cells are crucial for the diagnosis, management, and treatment of cancer. In this study, a fluorescence probe, HPQ, was used to detect GGT activity based on the known mechanism of excited-state intramolecular proton transfer (ESIPT). DFT and TDDFT calculations were performed to evaluate the sensing mechanism, and the results were cross-validated by frontier molecular orbital (FMO) analysis, geometric analysis, and potential energy curve (PEC) scanning.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Huihui Mei, Xin Gu, Minghui Wang, Jiajia Chen, Xindi Yang, Xuejun Liu, Kuoxi Xu
Summary: A new pH fluorescent probe based on reversible structural changes was developed, providing three reversible pH response modes within a wide pH range. It is suitable for monitoring pH and fluorescence imaging in living cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Polymer Science
Raju Lunkad, Philip Biehl, Anastasiia Murmiliuk, Pablo M. Blanco, Peter Mons, Miroslav Stepanek, Felix H. Schacher, Peter Kosovan
Summary: We synthesized three different polyzwitterions and investigated their ionization states in response to changes in solution pH. By using molecular simulations and experimental measurements, we predicted and explained the differences in ionization behavior and effectively obtained pK(a) values.
Article
Chemistry, Multidisciplinary
Yanju Luo, Chuan Li, Wencheng Zhu, Xujun Zheng, Yan Huang, Zhiyun Lu
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Biochemistry & Molecular Biology
Jialiang Shao, Jiongjiong Lu, Wencheng Zhu, Hua Yu, Xiaoqian Jing, Yi-Lin Wang, Xiang Wang, Xiong-Jun Wang
CELL DEATH AND DIFFERENTIATION
(2019)
Article
Chemistry, Multidisciplinary
Xujun Zheng, Wencheng Zhu, Chi Zhang, Yang Zhang, Cheng Zhong, Hao Li, Guohua Xie, Xiongjun Wang, Chuluo Yang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Multidisciplinary Sciences
Wenjuan Wang, Zihou Deng, Hongyu Wu, Qun Zhao, Tiantian Li, Wencheng Zhu, Xiongjun Wang, Longhai Tang, Chengshu Wang, Shu-Zhong Cui, Hui Xiao, Jiangye Chen
NATURE COMMUNICATIONS
(2019)
Article
Engineering, Biomedical
Rongrong Jin, Li Liu, Wencheng Zhu, Danyang Li, Li Yang, Jimei Duan, Zhongyuan Cai, Yu Nie, Yunjiao Zhang, Qiyong Gong, Bin Song, Longping Wen, James M. Anderson, Hua Ai
Article
Engineering, Biomedical
Li Liu, Rongrong Jin, Jimei Duan, Li Yang, Zhongyuan Cai, Wencheng Zhu, Yu Nie, Jing He, Chunchao Xia, Qiyong Gong, Bin Song, James M. Anderson, Hua Ai
ACTA BIOMATERIALIA
(2020)
Article
Biochemistry & Molecular Biology
Wencheng Zhu, Xueyi Fan, Qun Zhao, Yinxing Xu, Xiongjun Wang, Jiangye Chen
Summary: This study demonstrates the importance of histone H2B mono-ubiquitination in the morphological plasticity and pathogenicity of the human fungal pathogen Candida albicans. The dynamic regulation of H2B ubiquitination and deubiquitination by E3 ligase Bre1 and deubiquitinase Ubp8 plays a crucial role in controlling the expression of hypha-specific genes during the reversible yeast-hyphae transition. Additionally, Ubp8 contributes to the pathogenicity of C. albicans during early infection in a mouse model.
MOLECULAR MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zhouyue Lei, Wencheng Zhu, Xingcai Zhang, Xiongjun Wang, Peiyi Wu
Summary: The research developed a biomimetic hydrogel to address the key challenges encountered by traditional synthetic hydrogel-based ionic skins, providing stability, adhesion, hydration, sensitive detection, and enhanced treatment efficacy in ambient conditions.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Oncology
Changqiang Wu, Wencheng Zhu, Rongrong Jin, Hua Ai, Ye Xu
Summary: This study aimed to modulate the in vivo behaviors of dendritic cell vaccines by introducing siRNA-laden magnetic resonance imaging nanovectors to improve their efficacy in treating cancers. The use of N-alkyl-PEI2k-LAC/SPIO nanocomposites was shown to be effective for siRNA transfection and MRI labeling in dendritic cells, resulting in better tumor suppression in an orthotopic mouse model of breast cancer. Overall, the nanocomposites represent a highly efficient MR imaging platform for siRNA transfection and in vivo tracking of vaccine cells.
FRONTIERS IN ONCOLOGY
(2021)
Review
Chemistry, Inorganic & Nuclear
Haitao Yu, Yan Guo, Wencheng Zhu, Kaden Havener, Xujun Zheng
Summary: This review highlights the recent advances in the imaging and tracking of organelles in living cells using 1,8-naphthalimide (NI) fluorophore, focusing on their labeling mechanism and applications for monitoring various aspects of the cell microenvironment. It also discusses the limitations of the reported NI-based organelle fluorescent probes and offers insights into future developments in this field. The review aims to serve as a reference for beginners and a guide for chemical and biological researchers in exploring more promising research topics.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Qun Zhao, Baodi Dai, Hongyu Wu, Wencheng Zhu, Jiangye Chen
Summary: This study demonstrated the critical roles of Ino80 in hyphal development, DNA damage response, and pathogenesis in C. albicans.
MOLECULAR MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kaixin Yu, Yufeng Ding, Hua Yu, Wencheng Zhu, Haitao Yu, Yanju Luo, Xujun Zheng, Yan Huang, Zhiyun Lu, Xiongjun Wang
Summary: The observation and discovery of lysosome dynamic alterations using a lysosome-targetable fluorescent probe have led to a better understanding of lysosome biology and the development of new cancer therapeutics. By studying the positioning of lysosomes, a potential anticancer therapy combining TNF alpha and chloroquine has been revealed, with the mechanism potentially involving lysosomal dilation.
Article
Pharmacology & Pharmacy
Xumiao Li, Wencheng Zhu, Yi Shen, Qinqin Wang, Wenjun Liu, Junfeng Zhang, Huiping Zhang, Jingquan Li, Ziyi Li, Zhen Liu, Chen Li, Hui Wang
Summary: Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, the single-cell or low-input proteomic research in this field is relatively underdeveloped. In this study, a comprehensive solution of ultrasensitive proteome technology (CS-UPT) was developed for single-cell or low-input mouse oocyte/ embryo samples. The deep coverage and high-throughput routes significantly reduced the starting material and provided rich insights into minor zygotic genome activation.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Wencheng Zhu, Yufeng Ding, Juan Meng, Lei Gu, Wenjun Liu, Li Li, Hongyu Chen, Yining Wang, Ziyi Li, Chen Li, Yidi Sun, Zhen Liu
Summary: In this study, the dynamic changes of N-6-methyladenosine (m(6)A) modification during maternal-to-zygotic transition (MZT) in mice were investigated through multi-omic analysis. The results show that m(6)A modification can be inherited from the mother or gained de novo after fertilization. Interestingly, m(6)A modification on maternal mRNAs not only correlates with mRNA degradation, but also maintains the stability of a small group of mRNAs and promotes their translation after fertilization. Ythdc1 and Ythdf2 were identified as key m(6)A readers for mouse preimplantation development. This study reveals the crucial role of m(6)A-mediated RNA metabolism during MZT in mammals.
Article
Cell Biology
Wencheng Zhu, Huiying Chu, Yajuan Zhang, Tianhang Luo, Hua Yu, Hongwen Zhu, Ye Liu, Hong Gao, Yun Zhao, Quanlin Li, Xiongjun Wang, Guohui Li, Weiwei Yang
Summary: Emerging evidence suggests that certain metabolic enzymes have the ability to phosphorylate protein substrates in addition to soluble metabolites, playing a role as protein kinases to regulate crucial cellular processes such as cell cycle and apoptosis. This study identifies the metabolic enzyme fructose 1,6-biphosphatase 1 (FBP1) as a protein phosphatase that dephosphorylates the serine (S) 32/36 of I kappa B alpha, inhibiting NF-kappa B activation. The findings also highlight the important role of FBP1-mediated I kappa B alpha dephosphorylation in suppressing colorectal tumorigenesis.
