期刊
RSC ADVANCES
卷 4, 期 102, 页码 58852-58857出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra12478b
关键词
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资金
- National Basic Research Program of China [2010CB732403]
- National Nature Sciences Funding of China [21175024, 21222506, 21375021]
- program for New Century Excellent Talents in University [NCET-12-0619]
- Natural Sciences Funding of Fujian Province [2012J06005, 2014J06005]
A signal on fluorescence biosensor for ultrasensitive detection of MMP-2 based on fluorescence resonance energy transfer (FRET) between semiconducting polymer dots (Pdots) and a metal-organic framework (MOF) has been developed. Carboxyl-rich PFO has been modified firmly with a polypeptide chain (COOH-GHHYYGPLGVRGC-NH2) through a carboxy ammonia reaction first. The polypeptide chain comprises the specific MMP-2 substrate domain (PLGVR) and the pi-rich motif (HHYY) can be absorbed by the MOF (H(2)dtoaCu used in this study) through pi-staking interactions. Hence, the fluorescence from Pdots can be quenched by the MOF with the assistance of the polypeptide chain linker through the FRET process. Upon the cleavage of the substrate by the protease (MMP-2) at the amide bond between Gly and Val, the Pdots has been separated from the MOF surface and therefore the FRET process can be inhibited, and the fluorescence of the system recovered. Under optimal conditions, the fluorescence recovery was found to be proportional to the concentration of MMP-2 within the range of 0.1 to 2.5 pg mL(-1).
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