Article
Chemistry, Medicinal
Shamsunnahar Khushi, Angela A. Salim, Ahmed H. Elbanna, Laizuman Nahar, Robert J. Capon
Summary: Thorectandra choanoides (CMB-01889) was identified as a potential source of new chemistry from marine sponge extracts in southern Australia, leading to the discovery of a new alkaloid. Detailed spectroscopic analysis and biosynthetic origins shed light on pharmaceutical potential, with insights into the specificity of IDO substrates and the reactive transformation of Michael acceptors.
Article
Engineering, Marine
Ying Zhou, Huixian Liang, Zhaocong Wang, Xiaojian Liao, Shihai Xu, Bingxin Zhao
Summary: A new cadinene sesquiterpene, Clionaterpene (1), along with six known compounds 2-7, were isolated from the marine sponge Cliona sp. The structure and absolute configuration of compound 1 were determined using spectroscopic data and quantum chemical calculation. The cytotoxic and antibacterial activities of these compounds were evaluated, but none of them showed significant inhibition effects.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Sophia A. Kolesnikova, Ekaterina G. Lyakhova, Anastasia B. Kozhushnaya, Anatoly I. Kalinovsky, Dmitrii V. Berdyshev, Roman S. Popov, Valentin A. Stonik
Summary: Two new isomalabaricane triterpenoids and four new isomalabaricane-derived nor-terpenoids were isolated in the study, along with the correction in stereochemistry of two known isomalabaricanes.
Article
Ecology
Florian U. U. Moeller, Craig W. W. Herbold, Arno Schintlmeister, Maria Mooshammer, Cherie Motti, Bettina Glasl, Katharina Kitzinger, Faris Behnam, Margarete Watzka, Thomas Schweder, Mads Albertsen, Andreas Richter, Nicole S. S. Webster, Michael Wagner
Summary: Marine sponges play a critical role in marine ecosystems as filter-feeders and reef-builders, and they harbor dense communities of microbes. These microbes contribute to the processing of dissolved organic matter (DOM). A study using metaproteogenomics and laboratory incubations found that a dominant gammaproteobacterial symbiont in a marine sponge expressed a pathway for the import and dissimilation of taurine, a common metabolite in marine sponges. The symbiont also imported and used DMSP as a carbon and sulfur source. These findings highlight the importance of biogenic sulfur compounds in the interaction between marine sponges and their microbial symbionts.
Article
Biochemistry & Molecular Biology
Mei-Hong Zhou, Xiang-Chao Luo, Hui-Min Zhao, Jing-Rong Lu, Ying Dai, Yong Yu, Liang Zhang, Hou-Wen Lin, Fan Yang
Summary: This study isolated three new spiro-sesquiterpenoids from a marine sponge collected from the South China Sea. The structures of these compounds were determined through various experimental techniques, revealing their unique spiro skeleton. This research expands the chemical diversity of spiro-metabolites from marine sources.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Xin-Lian Liu, Ya-Fang Ding, Shu-Ping Wang, Li Liu, Jie Wang, Fan Yang
Summary: Three new polyacetylenes, pellynols P (1), Q (2), and R (3) were isolated from the marine sponge Petrosia sp., along with the known compound pellynol H (4). These compounds exhibited potent cytotoxicity against human hepatocellular carcinoma HepG2, human melanoma A375, and human colorectal carcinoma HT29 cell lines with IC50 values ranging from 1.4 to 4.4 μM.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Medicinal
Kuei-Hung Lai, Zheng-Hao Huang, Mohamed El-Shazly, Bo-Rong Peng, Wen-Chi Wei, Jui-Hsin Su
Summary: Marine sponges of the genera Geodia, Jaspis, Rhabdastrella, and Stelletta are chemically characterized by a variety of isomalabaricane triterpenes. Two new isomalabaricane-type triterpenes rhabdastin H and rhabdastin I were identified, showing anti-proliferative activities against leukemic cells. These compounds exhibit unique structural features with the only reported tetrahydrofuran functionality among marine-derived isomalabaricanes.
Article
Chemistry, Applied
Murtihapsari, Supriatno Salam, Dikdik Kurnia, Darwati, Kadarusman, Fajar Fauzi Abdullah, Tati Herlina, Muhammad Hafiz Husna, Khalijah Awang, Yoshihito Shiono, Mohamad Nurul Azmi, Unang Supratman
Summary: A new antimalarial sterol, kaimanol, and a known sterol, saringosterol, were isolated from an Indonesian marine sponge. Both compounds showed antiplasmodial activity, with IC50 values of 359 and 0.250 nM, respectively.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Medicinal
Yang Liu, Lijian Ding, Jiaxin He, Ziming Zhang, Yueting Deng, Shan He, Xiaojun Yan
Summary: A new chromone named aspergilluone A was isolated from marine-derived fungus Aspergillus sp. LS57, showing inhibitory effects on Mycobacterium tuberculosis and moderate antibacterial activity against Staphylococcus aureus. It also exhibited weak activity against gram-positive Bacillus subtilis and gram-negative pathogen Escherichia coli.
Article
Chemistry, Organic
Wei-Hua Jiao, Jia-Xin Li, Hong-Yan Liu, Xiang-Chao Luo, Tian-Yong Hu, Guo-Hua Shi, Dong-Dong Xie, Hai-Feng Chen, Bao-Hui Cheng, Hou-Wen Lin
Summary: An unusual secomeroterpenoid, dysambiol (1), was isolated from a Dysidea sp. marine sponge collected from the South China Sea. Dysambiol features a rare lactone bridge in its unprecedented secomeroterpenoid scaffold. The structure of 1 was determined through extensive spectroscopic analysis, Mosher's method, and electronic circular dichroism calculation. Dysambiol exhibited potent anti-inflammatory activity in LPS-induced Raw 264.7 macrophages by regulating the NF-κB/MAKP signaling pathway.
Article
Chemistry, Organic
Wei-Hua Jiao, Jia-Xin Li, Hong-Yan Liu, Xiang-Chao Luo, Tian-Yong Hu, Guo-Hua Shi, Dong-Dong Xie, Hai-Feng Chen, Bao-Hui Cheng, Hou-Wen Lin
Summary: An unusual secomeroterpenoid, dysambiol (1), was isolated from a Dysidea sp. marine sponge collected from the South China Sea. Dysambiol features an unprecedented secomeroterpene scaffold with a rare lactone bridge. Dysambiol displayed potent anti-inflammatory activity in LPS-induced Raw 264.7 macrophages by regulating the NF-κB/MPAK signaling pathway.
Article
Biochemistry & Molecular Biology
Chi-Jen Tai, Chih-Hua Chao, Atallah F. Ahmed, Chia-Hung Yen, Tsong-Long Hwang, Fang-Rong Chang, Yusheng M. Huang, Jyh-Horng Sheu
Summary: Chemical investigation of the Red Sea sponge Spongia sp. resulted in the discovery of four new compounds, secodinorspongins A-D (1-4), as well as a known compound sponginolide (5). The structures of these compounds and their absolute configurations were elucidated using spectroscopic methods and a comparison of calculated and experimental CD spectra. A possible biosynthetic pathway for compounds 1-4 was proposed. The cytotoxic, antibacterial, and anti-inflammatory activities of compounds 1-5 were also evaluated, with compound 1 showing inhibitory activity against Staphylococcus aureus growth, and compounds 4 and 5 suppressing superoxide-anion generation and elastase release in human neutrophils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Jabal Rahmat Haedar, Agustinus Robert Uria, Subehan Lallo, Dya Fita Dibwe, Toshiyuki Wakimoto
Summary: This study reported three new members of the Theonellapeptolide family from marine sponge Theonella swinhoei collected in Indonesia, which showed selective cytotoxic activity against human pancreatic cancer cells in a nutrient-deprived medium.
Article
Plant Sciences
Qihao Wu, Kurt Throckmorton, Mitasree Maity, Marc G. Chevrette, Doug R. Braun, Scott R. Rajski, Cameron R. Currie, Michael G. Thomas, Tim S. Bugni
Summary: Chemical investigation of a marine sponge-associated Bacillus sp. led to the discovery of bacillibactins E and F, the first bacterial siderophores containing nicotinic and benzoic acid moieties. The structures and absolute configurations of these new compounds were determined through extensive spectroscopic analyses and Marfey's method, with a proposed biosynthetic pathway that differentiates itself by a promiscuous DhbE enzyme.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Dai-Chun Li, Hui-Xian Liang, Xiao-Jian Liao, Xi-Wen Xing, Shi-Hai Xu, Bing-Xin Zhao
Summary: Two new pairs of enantiomeric butenolides, (+)- and (-)-suberiteslide A, (+)- and (-)-subertieslide B, were isolated from the marine sponge Suberties sp. The structures and absolute configurations of these compounds were determined using spectroscopic analyses and ECD (Electronic Circular Dichroism). This is the first discovery of butenolides from marine sponges of the genus Suberites. Additionally, the anti-inflammatory, antibacterial, and cytotoxic activities of these compounds were evaluated, and only (-)-subertieslide B exhibited weak anti-inflammatory activity with an IC50 value of 40.8 μM.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Chemistry, Medicinal
Amila Agampodi Dewa, Ahmed H. Elbanna, Zeinab G. Khalil, Robert J. Capon
Summary: This report presents a structure-activity relationship (SAR) analysis method for a newly discovered P-gp inhibitory pharmacophore, and successfully isolated structurally diverse pharmacophores using an innovative precursor-directed biosynthesis strategy. The study provides key structure requirements for designing P-gp inhibitors, and identifies non-essential sites that allow for the design of affinity and other conjugated probes.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Plant Sciences
Taizong Wu, Angela A. Salim, Hui Cui, Zeinab G. Khalil, Paul Bernhardt, Robert J. Capon
Summary: Chemical investigations into a pasture plant-derived Streptomyces sp. from an Australian sheep station yielded rare compounds with potential anticancer properties that can reverse drug resistance in cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Sarani Kankanamge, Zeinab G. Khalil, Thulasi Sritharan, Robert J. Capon
Summary: This study used GNPS and MATRIX technologies to discover a new series of indole diterpenes from an Australian marine-derived fungus, with potential application in the treatment of glaucoma, cancer, neurodegenerative diseases, as well as insecticides and antivirals.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Immunology
Jieru Yang, Jennifer C. Boer, Mattaka Khongkow, Sarunya Phunpee, Zeinab G. Khalil, Sahra Bashiri, Cyril Deceneux, Georgia Goodchild, Waleed M. Hussein, Robert J. Capon, Uracha Ruktanonchai, Magdalena Plebanski, Istvan Toth, Mariusz Skwarczynski
Summary: Intranasal vaccine administration has advantages in mass immunization. A new liposomal system using lipidated chitosan derivative (OTMC) showed promising results in stimulating high systemic antibody titers against group A Streptococcus (GAS) in mice. OTMC was more effective than the traditional trimethyl chitosan formulation and could enhance the immune responses of the vaccine without the need for a liposome delivery system. Importantly, OTMC also stimulated cytokine production by dendritic cells, indicating its potential for mucosal vaccine development.
Review
Biotechnology & Applied Microbiology
Mark S. Butler, Ian R. Henderson, Robert J. Capon, Mark A. T. Blaskovich
Summary: The global need for new antibacterial drugs to combat drug-resistant bacterial infections is receiving significant attention, leading to numerous funding, policy, and legislative initiatives to support antibacterial research and development. This review assesses the impact of these programs by analyzing the clinical development of various types of antibacterial drugs and the number of drug approvals in recent years. The findings show an increasing number of early-stage candidates and a presence of novel antibacterial compounds, particularly targeting Gram-negative bacteria.
JOURNAL OF ANTIBIOTICS
(2023)
Article
Plant Sciences
Kaumadi Samarasekera, Waleed M. Hussein, Taizong Wu, Angela A. Salim, Robert J. Capon
Summary: Chemical analysis of a fungus derived from a Queensland mud dauber wasp nest revealed the presence of duclauxin (1) and a group of new compounds called glyclauxins A-E (2-6). The structures and absolute configuration of these compounds were determined through spectroscopic analysis, synthetic conversions, and chemical interconversion. Despite their potential as artifacts, the detection of glyclauxins B (3) and A (2) in a fresh culture extract confirmed their natural product status.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Medicinal
Shamsunnahar Khushi, Angela A. A. Salim, Robert J. J. Capon
Summary: In reviewing recent case studies, we found that the application of mass spectrometry molecular networking has provided valuable lessons and benefits in the field of marine sponge natural products. Molecular networking played a crucial role in discovering new natural products and even new classes of natural products that were not detected by other dereplication and prioritization strategies. These case studies include the discovery of rare trachycladindoles, unprecedented dysidealactams, novel cacolides, and a new class of indole alkaloids called thorectandrins, which provided unexpected insights into the properties of aplysinopsins.
Article
Chemistry, Medicinal
Angela A. Salim, Waleed M. Hussein, Pradeep Dewapriya, Huy N. Hoang, Yahao Zhou, Kaumadi Samarasekera, Zeinab G. Khalil, David P. Fairlie, Robert J. Capon
Summary: The application of a miniaturized 24-well plate system allowed for the optimization of cultivation conditions for the marine-derived fungus Talaromyces sp. CMB-TU011, leading to the discovery of a rare cycloheptapeptide talarolide A and three new analogues. Detailed spectroscopic analysis, supported by Marfey's analysis methodology, helped confirm the structures of these compounds. The investigation of hydroxamates' influence on cyclic peptide conformation through ROESY correlations and solution structure determination offers insights into the potential use of hydroxamates as molecular bridges for accessing new cyclic peptide conformations.
Article
Chemistry, Medicinal
Shengbin Jin, Jiahui Zhang, Ummey J. Nahar, Wenbin Huang, Nedaa A. Alharbi, Ahmed O. Shalash, Prashamsa Koirala, Jieru Yang, Jolynn J. E. Kiong, Zeinab G. Khalil, Robert J. Capon, Rachel J. Stephenson, Mariusz Skwarczynski, Istvan Toth, Waleed M. Hussein
Summary: Untreated group A Streptococcus (GAS) infections can be life-threatening, but there are no commercially available vaccines to treat or prevent them. Peptide-based subunit vaccines, combined with immunestimulants and/or vaccine delivery platforms, show promise in improving immunogenicity. This study investigated the use of polyelectrolyte complexes (PECs) and polymer-coated liposomes as self-adjuvanting vehicles for GAS peptide antigens delivery. The results showed that both PECs and liposomes induced strong immune responses, with liposomes having more pronounced effects.
ACS INFECTIOUS DISEASES
(2023)
Review
Biochemistry & Molecular Biology
Angela A. Salim, Mark S. Butler, Mark A. T. Blaskovich, Ian R. Henderson, Robert J. Capon
Summary: This review summarizes all natural products (500 compounds, including 17 semi-synthetic derivatives) reported in the primary literature up to December 2022 that have been shown to inhibit the egg hatching, motility, larval development, and/or survival of helminths (nematodes, flukes, and tapeworms). These parasites infect and harm the health, welfare, productivity, and lives of commercial livestock, companion animals, and other valuable, endangered, and exotic animals. The review focuses on chemical structures, source organisms, and anthelmintic properties, including the target species, animal hosts, and potency measures used in bioassays.
NATURAL PRODUCT REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Mariusz Skwarczynski, Guangzu Zhao, Victoria Ozberk, Ashwini Kumar Giddam, Zeinab G. Khalil, Manisha Pandey, Waleed M. Hussein, Reshma J. Nevagi, Michael R. Batzloff, Robert J. Capon, Michael F. Good, Istvan Toth
Summary: Peptide-based vaccines are composed of minimal microbial components that are required to stimulate immune responses. Peptides themselves are poor immunogens and usually need to be co-administered with an adjuvant to produce desired immune responses. Recent research has shown that poly(hydrophobic amino acids) can self-assemble into nanoparticles and induce strong humoral immune responses when conjugated with a peptide antigen.
AUSTRALIAN JOURNAL OF CHEMISTRY
(2022)