Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nadia Nascimento da Rosa, Julia Maurer Appel, Ana Carolina Irioda, Bassam Felipe Mogharbel, Nathalia Barth de Oliveira, Maiara Carolina Perussolo, Priscila Elias Ferreira Stricker, Livia Rosa-Fernandes, Claudio Romero Farias Marinho, Katherine Athayde Teixeira de Carvalho
Summary: A 3D lung model was developed using Wharton's jelly mesenchymal stem cells (WJ-MSC) for pulmonary differentiation and bioprinting. The differentiation of WJ-MSC into lung cells and bioprinting in a 3D structure were successfully achieved, providing a promising alternative for in vitro drug testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jianmei Chen, Xiaotong Wu, Yu Zhang, Yurui Xu, Haixiong Ge, Xinghai Ning
Summary: CellMatrix is a biomimetic 3D dynamic culture system with rational structure and function that improves cell supply. It can be directly implanted into defected tissues and improve tissue remodeling.
Article
Engineering, Biomedical
Yan Xie, Linawati Sutrisno, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Interconnected scaffolds promote the chondrogenic differentiation of stem cells. In this study, collagen scaffolds with interconnected pore structures were fabricated using PLGA sponge templates and were used to culture human bone marrow-derived mesenchymal stem cells (hMSCs). The results showed that the interconnected scaffolds can enhance the chondrogenic differentiation of stem cells and improve the production of cartilaginous matrices and the mechanical properties of the implants after subcutaneous implantation.
BIOMEDICAL MATERIALS
(2022)
Review
Engineering, Biomedical
Woochan Kim, Yonghyun Gwon, Sunho Park, Hyoseong Kim, Jangho Kim
Summary: Three-dimensional stem cell culture systems have the potential to mimic complex interactions between cells and the extracellular matrix, and have unique properties that can be applied in therapeutics.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Environmental
Wenting Yu, Xiaoxia Su, Moxiao Li, Wanting Wan, Ang Li, Hong Zhou, Feng Xu
Summary: This study investigated the effects of matrix strain on the characteristics and bioactivity of MSC-derived exosomes by culturing PDLSCs in three-dimensional magnetically stretched collagen hydrogels. The results showed that altering the miRNA content in exosomes significantly enhanced their bioactivity, leading to improved repair of bone defects in vivo. This research suggests a novel strategy for promoting tissue regeneration through engineered mechanical microenvironments.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Hidekazu Sekine, Teruo Okano
Summary: One of the key challenges in regenerative medicine is to introduce vascular networks into bioengineered tissues. Co-culturing transplant tissue with vascular cells before transplantation has been explored as a way to promote capillary angiogenesis and construct a continuous circulatory structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Irfan Khan, Marium Naz Siddiqui, Fatima Jameel, Rida-e-Maria Qazi, Asmat Salim, Shazmeen Aslam, Midhat Batool Zaidi
Summary: Hypoxic wounds are difficult to heal and cause a burden on healthcare. Traditional skin substitutes lack proper vascularization. Transplanting genetically modified mesenchymal stem cells on a three-dimensional collagen scaffold can enhance skin regeneration, with improved proliferation, reduced inflammation, and enhanced vasculogenesis.
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Lauren E. E. Jansen, Hyuna Kim, Christopher L. Hall, Thomas P. McCarthy, Michael J. Lee, Shelly R. Peyton
Summary: Three-dimensional hydrogels made from synthetic polymers can mimic the extracellular matrix (ECM) of real tissues, but they have limitations in terms of the diversity and specificity of bioactive peptides. To overcome these limitations, researchers have developed a bone marrow hydrogel that incorporates the complex and specific protein signature of bone marrow. This tissue-centric approach allows for better replication of the elasticity and structure of bone marrow, and provides a wide range of bone marrow-specific and cell-instructive peptides.
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Moyassar Basil Hadi Al-Shaibani
Summary: The secretome of MSCs, known as MSC-CM, contains growth factors and cytokines that control cellular responses. Comparing MSC-CM generated from traditional 2DCC and 3DCC using a polystyrene scaffold, 3DCC showed higher concentrations of biomolecules secreted by MSCs, making it a novel approach for therapeutic applications.
BIOTECHNOLOGY LETTERS
(2022)
Article
Chemistry, Physical
Lingjiao Zou, Weiqing Zhou, Xiangming Na, Naoki Asakawa, Hua Yue, Guanghui Ma
Summary: 3D cell carriers with porous structures have the potential to investigate drug treatments for tumors. The fabrication of gigantic highly-porous microcarriers allows for high-efficiency 3D cell culture and the construction of artificial tumor tissue models.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)